FastQCFastQC Report
Wed 25 May 2016
SRR2049512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4345242
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42600.044.117051
GTATCAA67250.034.342691
ATCAACG69450.031.4073143
TCAACGC70000.031.0869884
CAACGCA75200.029.1408635
TATCAAC79500.027.8233932
AACGCAG83250.026.5075456
GTACATG102300.024.077951
GTGGTAT25900.023.330941
TACCTGG30300.023.1510892
ACGCAGA94650.023.0981967
CGCAGAG94000.022.6602528
TACATGG105650.022.5844672
GTACCTG32350.022.0034391
GTCTCGC14000.021.2153421
TGGTATC26350.021.180652
ACATGGG105750.021.062133
TCGCTCT15200.019.2007884
GTACGAC1901.05072315E-418.8641783
GCAGAGT111450.018.3412979
Produced by FastQC (version 0.11.5)