FastQCFastQC Report
Wed 25 May 2016
SRR2049512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4345242
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182970.42108126543930113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169800.39077225158000406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106580.24527977958419808No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75600.1739834052971043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51000.11736975754169734No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46670.10740483498962773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91600.057.967741
GTATCAA162950.040.0677381
GTGGTAT35850.033.0890351
ATCAACG200850.032.3961143
TCAACGC203000.032.076184
TATCAAC207450.031.3881132
CAACGCA210150.031.1533155
TGGTATC37900.030.0412982
AACGCAG220000.029.8646246
TATAACG4850.029.101952
ATAACGC4800.028.4249253
ACGCAGA247450.026.513717
CGCAGAG246400.026.5122558
GTACCTG33500.024.8714581
GCAGAGT266300.024.0192559
TACCTGG33500.024.0156232
TAACGCA6850.021.9786994
GTACATG101900.021.8042221
CAGAGTA261700.021.7656410-11
GAGTACT220300.021.14130212-13
Produced by FastQC (version 0.11.5)