##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049512_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4345242
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704227520584585	31.0	31.0	34.0	30.0	34.0
2	31.96767913041437	33.0	31.0	34.0	30.0	34.0
3	31.957384882130846	33.0	31.0	34.0	30.0	34.0
4	35.61709129203851	37.0	35.0	37.0	33.0	37.0
5	35.74869869158035	37.0	35.0	37.0	35.0	37.0
6	35.83214720837182	37.0	35.0	37.0	35.0	37.0
7	35.816807671471466	37.0	35.0	37.0	35.0	37.0
8	35.800784628336004	37.0	35.0	37.0	35.0	37.0
9	37.53381698878912	39.0	37.0	39.0	35.0	39.0
10-11	37.49251951905095	39.0	37.0	39.0	34.5	39.0
12-13	37.47703165899621	39.0	37.0	39.0	34.5	39.0
14-15	38.74419399425855	40.0	38.0	41.0	34.5	41.0
16-17	38.748794773685795	40.0	38.0	41.0	34.5	41.0
18-19	38.82909110700854	40.0	38.0	41.0	35.0	41.0
20-21	38.85280037337391	40.0	38.0	41.0	35.0	41.0
22-23	38.80900891135637	40.0	38.0	41.0	35.0	41.0
24-25	38.76356863438216	40.0	38.0	41.0	35.0	41.0
26-27	38.65859991227186	40.0	38.0	41.0	34.5	41.0
28-29	38.56269213544378	40.0	38.0	41.0	34.0	41.0
30-31	38.4219162016753	40.0	38.0	41.0	34.0	41.0
32-33	38.23585659901106	40.0	38.0	41.0	34.0	41.0
34-35	38.108008368693845	40.0	38.0	41.0	34.0	41.0
36-37	37.94847444630241	40.0	37.5	41.0	33.0	41.0
38-39	37.84254674423197	40.0	37.0	41.0	33.0	41.0
40-41	37.71045110951243	40.0	37.0	41.0	33.0	41.0
42-43	37.55889430324019	40.0	37.0	41.0	33.0	41.0
44-45	37.359829671166764	40.0	37.0	41.0	32.5	41.0
46-47	37.09282417411964	40.0	37.0	41.0	31.5	41.0
48-49	36.83751814053164	40.0	37.0	41.0	31.0	41.0
50-51	36.628673040534906	40.0	36.0	41.0	30.0	41.0
52-53	36.52573400975136	40.0	36.0	41.0	30.0	41.0
54-55	36.39347819983329	39.5	36.0	41.0	30.0	41.0
56-57	36.2304658750882	39.0	35.0	41.0	30.0	41.0
58-59	36.06583028056895	39.0	35.0	41.0	30.0	41.0
60-61	35.87379770792973	39.0	35.0	41.0	29.0	41.0
62-63	35.66270773871743	39.0	35.0	41.0	29.0	41.0
64-65	35.38058823881386	38.0	35.0	40.0	29.0	41.0
66-67	35.12961832735668	37.5	35.0	40.0	28.0	41.0
68-69	34.81295587219308	37.0	34.5	40.0	28.0	41.0
70-71	34.51398759838923	36.5	34.0	39.5	28.0	41.0
72-73	34.19113239262623	36.0	34.0	39.0	28.0	41.0
74-75	33.85223343141763	36.0	34.0	39.0	27.0	40.5
76-77	33.56389218368045	35.0	34.0	38.5	27.0	40.0
78-79	33.23862560474193	35.0	34.0	37.0	26.5	39.0
80-81	32.93021723531163	35.0	34.0	37.0	26.0	39.0
82-83	32.63990509619487	35.0	33.0	36.0	26.0	39.0
84-85	32.36262790426862	35.0	33.0	36.0	26.0	37.0
86-87	32.08970685637301	35.0	33.0	36.0	26.0	37.0
88-89	31.878775796606956	35.0	33.0	35.0	25.5	37.0
90-91	31.726747900347092	35.0	33.0	35.0	25.0	36.0
92-93	31.565503026068512	35.0	33.0	35.0	25.0	36.0
94-95	31.379581735608742	35.0	33.0	35.0	24.5	36.0
96-97	31.206834279885907	35.0	33.0	35.0	24.0	36.0
98-99	31.029110346443304	35.0	33.0	35.0	23.0	35.0
100	30.91706422795324	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43849951242277996
1101	2	0.44687567547396867
1101	3	0.5392377058898674
1101	4	0.3880393506194011
1101	5	0.3162479360250998
1101	6	0.31484917262101675
1101	7	0.33062443457278334
1101	8	0.3434401270066232
1101	9	0.3812108821905369
1101	10-11	0.4043494351188599
1101	12-13	0.40322890241382225
1101	14-15	0.4940586685308048
1101	16-17	0.5094996569353256
1101	18-19	0.47930893492528526
1101	20-21	0.4701886057451574
1101	22-23	0.4869577404248915
1101	24-25	0.5333348277637668
1101	26-27	0.4893983211809214
1101	28-29	0.4260226333904882
1101	30-31	0.4926531813161006
1101	32-33	0.5553779373609444
1101	34-35	0.4864049409045563
1101	36-37	0.53651001733828
1101	38-39	0.47413492767718424
1101	40-41	0.5218973559103759
1101	42-43	0.4190324071967737
1101	44-45	0.4673633824133887
1101	46-47	0.533508596444662
1101	48-49	0.5750118461734957
1101	50-51	0.6327694092034264
1101	52-53	0.6791652674223769
1101	54-55	0.5923550080041764
1101	56-57	0.5956016811774987
1101	58-59	0.5745252313188587
1101	60-61	0.5739807467822686
1101	62-63	0.5783728160953174
1101	64-65	0.6099125199785931
1101	66-67	0.638695661742581
1101	68-69	0.623182650602466
1101	70-71	0.6466148334385053
1101	72-73	0.6930416432126734
1101	74-75	0.6518266204587491
1101	76-77	0.6501511522426142
1101	78-79	0.6057709198481263
1101	80-81	0.5835514003962814
1101	82-83	0.5580381003254828
1101	84-85	0.6039790798405278
1101	86-87	0.6152304482462725
1101	88-89	0.5772448050233407
1101	90-91	0.5883846071223218
1101	92-93	0.6155147924917515
1101	94-95	0.5457426462994803
1101	96-97	0.5825462799551566
1101	98-99	0.6719215335217648
1101	100	0.6714646195542571
1102	1	0.3517597220377908
1102	2	0.4028324879618381
1102	3	0.41576544222771616
1102	4	0.3818890018135832
1102	5	0.3107286108092566
1102	6	0.29593307545739833
1102	7	0.2933338483043144
1102	8	0.35074096669802657
1102	9	0.3953902531560658
1102	10-11	0.35866239658209054
1102	12-13	0.3536179878463841
1102	14-15	0.4503206446708816
1102	16-17	0.4539359081134009
1102	18-19	0.4559973634757384
1102	20-21	0.4648678251589331
1102	22-23	0.46751579421628975
1102	24-25	0.45207952937258966
1102	26-27	0.4320838891921426
1102	28-29	0.4177616896967038
1102	30-31	0.5019559485562937
1102	32-33	0.5136434157671914
1102	34-35	0.5553409765949908
1102	36-37	0.6091234325095627
1102	38-39	0.5998687104561711
1102	40-41	0.5896629977083521
1102	42-43	0.600574801430227
1102	44-45	0.5633220656181379
1102	46-47	0.5615472681883062
1102	48-49	0.602942691216569
1102	50-51	0.6477457884427409
1102	52-53	0.6116960868910937
1102	54-55	0.5966290396121892
1102	56-57	0.631641941846496
1102	58-59	0.5627479792361996
1102	60-61	0.6009401806417713
1102	62-63	0.5454928000876649
1102	64-65	0.6165939629406765
1102	66-67	0.6246374256154894
1102	68-69	0.5359368312995159
1102	70-71	0.5543659594206289
1102	72-73	0.558155780622748
1102	74-75	0.5942402359625021
1102	76-77	0.5493611177172326
1102	78-79	0.5201976635220191
1102	80-81	0.5006803117337739
1102	82-83	0.55052798898231
1102	84-85	0.46389575005918715
1102	86-87	0.5106667988801874
1102	88-89	0.5112936520460636
1102	90-91	0.5198270956020252
1102	92-93	0.5392871759745077
1102	94-95	0.5773768951000875
1102	96-97	0.5809585977970606
1102	98-99	0.5796365475639824
1102	100	0.6897189802189985
1103	1	0.3674478995998882
1103	2	0.36911082826845387
1103	3	0.4238187813677925
1103	4	0.3864692827150833
1103	5	0.2488628815178373
1103	6	0.28491601294670943
1103	7	0.313074307348586
1103	8	0.33458970087852435
1103	9	0.33764856017756273
1103	10-11	0.3026954950531575
1103	12-13	0.32668825469762197
1103	14-15	0.41434752162481914
1103	16-17	0.38589625631097846
1103	18-19	0.3771585059536662
1103	20-21	0.4042642343066376
1103	22-23	0.3718063598040402
1103	24-25	0.42022328789476404
1103	26-27	0.40561371877441843
1103	28-29	0.4598831233416263
1103	30-31	0.4766218271641307
1103	32-33	0.50061421092893
1103	34-35	0.5031922201207237
1103	36-37	0.5397742550459697
1103	38-39	0.5328448250150402
1103	40-41	0.535887521891901
1103	42-43	0.4779382261680212
1103	44-45	0.5789293634577106
1103	46-47	0.6184694836705518
1103	48-49	0.6819755563807348
1103	50-51	0.6672514889078016
1103	52-53	0.5908218600442083
1103	54-55	0.576671345928581
1103	56-57	0.5919666013480551
1103	58-59	0.6149049273746741
1103	60-61	0.5678473089450051
1103	62-63	0.5398567277314257
1103	64-65	0.5689533177693491
1103	66-67	0.556678860919483
1103	68-69	0.6127247657039874
1103	70-71	0.626184145952088
1103	72-73	0.6094570627737355
1103	74-75	0.6114143191960295
1103	76-77	0.5888658849547213
1103	78-79	0.5359376870600912
1103	80-81	0.4972333838670835
1103	82-83	0.555843445823129
1103	84-85	0.5890917537713563
1103	86-87	0.5910200773604757
1103	88-89	0.590008390301648
1103	90-91	0.5676373808067368
1103	92-93	0.566197622420205
1103	94-95	0.6152201024995456
1103	96-97	0.5687141336511452
1103	98-99	0.6061517202888496
1103	100	0.6158413678298871
1104	1	0.26053317508236873
1104	2	0.2407224128134331
1104	3	0.31310712903810156
1104	4	0.23224468234241868
1104	5	0.18050645436244395
1104	6	0.2241372423296184
1104	7	0.20921433438459758
1104	8	0.22036950007082368
1104	9	0.2789921146001646
1104	10-11	0.21057761207877945
1104	12-13	0.2671486474093072
1104	14-15	0.3339373055821966
1104	16-17	0.3269196451433132
1104	18-19	0.3252289422500141
1104	20-21	0.3435940843469396
1104	22-23	0.3262223207813335
1104	24-25	0.33847224145717547
1104	26-27	0.35195496938879955
1104	28-29	0.3423169657067646
1104	30-31	0.3452914195904455
1104	32-33	0.3967470284815846
1104	34-35	0.36627284971422824
1104	36-37	0.40077159984220145
1104	38-39	0.43429555997848013
1104	40-41	0.4716369177583388
1104	42-43	0.4976783812569181
1104	44-45	0.4872370180131824
1104	46-47	0.3792951181546016
1104	48-49	0.4015492391586122
1104	50-51	0.44382195636736554
1104	52-53	0.4154321301713182
1104	54-55	0.34801048765783804
1104	56-57	0.41706808428315156
1104	58-59	0.44538096889302636
1104	60-61	0.3625743706998179
1104	62-63	0.4090292149989594
1104	64-65	0.5010288699506873
1104	66-67	0.46887862404345526
1104	68-69	0.48301492315487593
1104	70-71	0.49732782612676374
1104	72-73	0.5279267177579001
1104	74-75	0.5233251355727759
1104	76-77	0.4332770567434281
1104	78-79	0.40565373377015135
1104	80-81	0.42096906578306914
1104	82-83	0.4226076430036585
1104	84-85	0.4803142789921466
1104	86-87	0.5061467127144041
1104	88-89	0.41100654238201173
1104	90-91	0.4631566468492849
1104	92-93	0.44261574059062525
1104	94-95	0.4231174439958849
1104	96-97	0.449170986595508
1104	98-99	0.5714695437983401
1104	100	0.632433860261095
1105	1	0.2412479758514543
1105	2	0.2508594600597789
1105	3	0.3069911915184136
1105	4	0.232286786151775
1105	5	0.17789305741796113
1105	6	0.23340075526856907
1105	7	0.2439766675243007
1105	8	0.21762615605392455
1105	9	0.27291501534102025
1105	10-11	0.23705637993757023
1105	12-13	0.19511731320577042
1105	14-15	0.31869261343788935
1105	16-17	0.29711497024459277
1105	18-19	0.2576879553672029
1105	20-21	0.26390263255664337
1105	22-23	0.29080144618289694
1105	24-25	0.2999665008618351
1105	26-27	0.2838248339595282
1105	28-29	0.34015174356649425
1105	30-31	0.3144997325630854
1105	32-33	0.35469852663482015
1105	34-35	0.3082845401177323
1105	36-37	0.4246295144200616
1105	38-39	0.4574349993917295
1105	40-41	0.37659569934811543
1105	42-43	0.46516668612924406
1105	44-45	0.5235175592217303
1105	46-47	0.5256093824680335
1105	48-49	0.5997044500841184
1105	50-51	0.6393737520425802
1105	52-53	0.623933797103362
1105	54-55	0.576841306235572
1105	56-57	0.6086952671991241
1105	58-59	0.6526309400799946
1105	60-61	0.6652652525865221
1105	62-63	0.5668885114699904
1105	64-65	0.6140894737907203
1105	66-67	0.5966536710967532
1105	68-69	0.6547417536397546
1105	70-71	0.5951896296684751
1105	72-73	0.5999857982411854
1105	74-75	0.6738450893169272
1105	76-77	0.5846337638301833
1105	78-79	0.5220347149874627
1105	80-81	0.44316261721239414
1105	82-83	0.4546319005452091
1105	84-85	0.5542856213195009
1105	86-87	0.5713730241729493
1105	88-89	0.4697229060862895
1105	90-91	0.4713770781864852
1105	92-93	0.4391169771668828
1105	94-95	0.456557959172347
1105	96-97	0.481807055592288
1105	98-99	0.5554402491259687
1105	100	0.5377703678820396
1106	1	0.20500769720632306
1106	2	0.2030555831652876
1106	3	0.28329151041725
1106	4	0.27615351512654485
1106	5	0.22548684350725523
1106	6	0.23319676428808833
1106	7	0.16659249324344927
1106	8	0.22315766973891016
1106	9	0.23326987874411031
1106	10-11	0.24067704326719053
1106	12-13	0.17747074501210847
1106	14-15	0.3046996155230062
1106	16-17	0.28968836943548837
1106	18-19	0.2875624248269233
1106	20-21	0.2789344627262267
1106	22-23	0.2748662961804982
1106	24-25	0.2828715173448373
1106	26-27	0.2888936277827554
1106	28-29	0.2717672185778284
1106	30-31	0.28882146094777994
1106	32-33	0.30382916677354643
1106	34-35	0.30897112354683287
1106	36-37	0.34970251005938735
1106	38-39	0.3038310752653288
1106	40-41	0.32023868033117964
1106	42-43	0.33709354627721666
1106	44-45	0.3561839890867802
1106	46-47	0.2756507649631317
1106	48-49	0.2525173553612916
1106	50-51	0.36182697569721256
1106	52-53	0.29484269923893436
1106	54-55	0.2648418530366996
1106	56-57	0.3222700033431778
1106	58-59	0.3025889026563533
1106	60-61	0.312389814474642
1106	62-63	0.34301547335019933
1106	64-65	0.3092958398503143
1106	66-67	0.32721369022579694
1106	68-69	0.3392774394764402
1106	70-71	0.32377378715234073
1106	72-73	0.3333796033105685
1106	74-75	0.3214685205543759
1106	76-77	0.31884751375971376
1106	78-79	0.2754049965643617
1106	80-81	0.18365523777286796
1106	82-83	0.20805939205714452
1106	84-85	0.20740804454485584
1106	86-87	0.2949948458271834
1106	88-89	0.3181523745835264
1106	90-91	0.29828870867734025
1106	92-93	0.2927698860962451
1106	94-95	0.2834373248008504
1106	96-97	0.2967692578678687
1106	98-99	0.3591321584083502
1106	100	0.41886006368570605
1107	1	0.13740305791067797
1107	2	0.16347433322556526
1107	3	0.20774607535015122
1107	4	0.1762011684968101
1107	5	0.15981989129773666
1107	6	0.12658022066481323
1107	7	0.12793762230588612
1107	8	0.14358306150077027
1107	9	0.16823047089541632
1107	10-11	0.15456962548445574
1107	12-13	0.1298782150814688
1107	14-15	0.1996787994071525
1107	16-17	0.1236546246437058
1107	18-19	0.15413073435033908
1107	20-21	0.22672694603077304
1107	22-23	0.18024013089521418
1107	24-25	0.20680200002966131
1107	26-27	0.18694581461751625
1107	28-29	0.25666957005047664
1107	30-31	0.27788470847988833
1107	32-33	0.28516775686273377
1107	34-35	0.21523282461910753
1107	36-37	0.3110181623780548
1107	38-39	0.26931260282314895
1107	40-41	0.31246595021864465
1107	42-43	0.3039476083024937
1107	44-45	0.3060576646773896
1107	46-47	0.3002374091888953
1107	48-49	0.27065488219120226
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2216	80-81	-1.375409679661331
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2216	84-85	-1.4742264934452791
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2216	90-91	-1.433027945315704
2216	92-93	-1.4204672502113596
2216	94-95	-1.4651200214275093
2216	96-97	-1.6630979209794639
2216	98-99	-1.8123293370568057
2216	100	-1.7940559873209736
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	219.0
3	24.0
4	59.0
5	111.0
6	386.0
7	988.0
8	2217.0
9	3419.0
10	4955.0
11	6806.0
12	10274.0
13	14357.0
14	15417.0
15	18379.0
16	22599.0
17	31990.0
18	32257.0
19	17709.0
20	13110.0
21	12116.0
22	12682.0
23	13701.0
24	15879.0
25	19567.0
26	24350.0
27	31625.0
28	40384.0
29	53055.0
30	68638.0
31	88181.0
32	113601.0
33	147433.0
34	202011.0
35	293601.0
36	461249.0
37	862869.0
38	1209761.0
39	477799.0
40	1464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8688867734488	20.326024933025135	14.171429860956636	22.633658432569426
2	16.303510327172102	21.21875543698892	38.96138207452944	23.516352161309538
3	18.04238245933885	25.790422211334192	31.046540341490843	25.120654987836115
4	12.697381351150275	18.03225657058449	38.16944572512539	31.10091635313984
5	12.007172088232059	37.01510041706001	35.305711296522254	15.672016198185668
6	29.09468841092356	38.44638815717101	17.942141840909624	14.516781590995812
7	25.365376657962894	33.03153196070552	23.132244418147483	18.470846963184098
8	24.525101248676137	34.83702403686607	21.5306995559741	19.107175158483695
9	24.120935036529612	16.614793836568825	22.861005209836414	36.40326591706515
10-11	23.027819854452293	26.86391229763498	29.266678357615067	20.841589490297665
12-13	24.37376891178188	24.533426339298554	29.523701907443105	21.569102841476457
14-15	21.662322258112066	26.581658713712166	27.736450951333996	24.019568076841775
16-17	20.066040050243462	29.68611414508099	28.236275447949733	22.011570356725816
18-19	19.768346619129616	27.527028414067615	31.848490832041115	20.856134134761653
20-21	21.132079640213362	27.091149353706882	31.531834130297	20.244936875782752
22-23	20.68323237232817	27.166864354160253	31.637674035186077	20.512229238325506
24-25	20.519662656303147	26.985424517207555	31.810081003543644	20.684831822945647
26-27	20.3204792736515	26.81716001088087	31.90895927085304	20.95340144461459
28-29	20.479187136893795	26.919934222177012	31.864375067530894	20.7365035733983
30-31	20.91483503237975	27.5009858508114	31.281797068809798	20.302382047999053
32-33	20.20512786287343	27.15948321393451	31.895929362721194	20.73945956047087
34-35	20.371937857546254	26.656478511438486	31.893114353584913	21.078469277430347
36-37	21.134104843872908	27.299779851156735	30.975927232591417	20.590188072378936
38-39	20.928868863920584	27.313634085282246	31.171129248957826	20.586367801839344
40-41	20.57537877061853	27.096845238999347	31.361509899793845	20.96626609058828
42-43	20.566725627709573	27.223282385653093	31.056106886566965	21.153885100070376
44-45	20.970477593653012	27.44750464991363	30.7740512496197	20.80796650681366
46-47	20.921803664790133	27.913727244650588	30.127712104412137	21.036756986147147
48-49	20.911597098619595	27.543701823741923	30.20372628267885	21.340974794959635
50-51	21.034639727775804	27.466053674340806	29.986247026057466	21.513059571825917
52-53	21.014088513367035	27.66249842931648	29.789710216369997	21.53370284094649
54-55	21.15793550738946	28.253351596988153	29.527975657052014	21.060737238570372
56-57	20.79367501280711	27.906316840350893	29.564590418669432	21.735417728172564
58-59	20.601749089174273	27.37475757916779	30.13619752956861	21.887295802089323
60-61	21.14287305517161	27.866744821116985	29.53076031208388	21.45962181162752
62-63	20.932643107104276	27.795736117804253	29.648003494396857	21.623617280694607
64-65	20.840507847434043	27.516096917041676	29.95057582523597	21.692819410288312
66-67	20.80494020816332	27.563079340575275	29.64532240091576	21.986658050345643
68-69	21.422194994477866	27.876681638601863	29.324196287386588	21.37692707953368
70-71	21.392272283108742	27.483912288429508	29.456989967417236	21.666825461044517
72-73	21.027252337154064	27.686202517604315	29.7932082954183	21.493336849823326
74-75	21.09820350627192	27.68440745072426	29.48207487638203	21.735314166621787
76-77	21.172779329666795	27.867538793006236	29.135672995888378	21.824008881438594
78-79	21.291389154857086	28.05824908684466	29.209383473001637	21.44097828529662
80-81	21.10645530067183	27.705315774605587	29.40362731295427	21.784601611768313
82-83	20.914189819191158	27.76866879950622	29.511674252964376	21.80546712833825
84-85	21.039944380543133	27.76803915639221	29.265343564294007	21.926672898770654
86-87	21.236043930349563	27.902358487743605	29.131438479145693	21.730159102761135
88-89	20.92743104263873	27.75675541014483	29.231046988319054	22.084766558897385
90-91	20.723902727088515	27.610429208891524	29.2671506984024	22.39851736561756
92-93	20.99009520464851	27.876722424890716	28.984192624605644	22.148989745855125
94-95	20.847524698700422	27.651394285465734	29.2005799017932	22.300501114040642
96-97	20.7184778201076	27.686363613349958	29.278944647962067	22.31621391858037
98-99	20.707309444976698	27.67559958124302	29.18326049119025	22.433830482590032
100	20.949618843430766	27.723076243281085	28.93448304748508	22.39282186580306
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1466.0
1	1219.5
2	3658.0
3	5717.0
4	3175.0
5	1313.0
6	2384.0
7	5395.5
8	8578.0
9	9608.5
10	7193.5
11	4240.5
12	3654.0
13	3957.5
14	4419.0
15	4903.5
16	5402.5
17	6043.5
18	6710.0
19	7397.0
20	8539.5
21	10162.0
22	12572.5
23	16296.5
24	20830.5
25	26324.0
26	34025.0
27	43594.0
28	56296.5
29	70007.0
30	81927.0
31	93908.5
32	104836.5
33	114657.0
34	124393.0
35	132369.5
36	137735.0
37	142975.0
38	147357.0
39	149298.0
40	150725.5
41	149837.0
42	145974.5
43	143082.0
44	140897.5
45	138816.5
46	137192.0
47	135363.0
48	133915.0
49	133621.0
50	131859.0
51	126482.5
52	118438.0
53	111029.5
54	106113.5
55	100362.5
56	93461.0
57	86080.0
58	78302.0
59	70825.5
60	61632.5
61	50908.0
62	41390.0
63	33014.5
64	25676.5
65	19774.0
66	15132.0
67	11423.5
68	8436.0
69	6099.0
70	4379.0
71	3211.5
72	2367.5
73	1745.5
74	1304.5
75	933.5
76	600.5
77	394.5
78	268.5
79	159.0
80	95.5
81	46.5
82	26.5
83	15.0
84	6.0
85	6.5
86	5.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059099124973937
2	1.380820676961145E-4
3	2.3013677949352416E-5
4	0.11743879857554539
5	0.0014728753887585547
6	0.0014728753887585547
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.3013677949352416E-5
14-15	3.452051692402863E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.9561626256949557E-4
30-31	2.4164361846820042E-4
32-33	2.3013677949352416E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.0712310154417175E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.3013677949352416E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.4958890667079072E-4
80-81	3.3369833026561006E-4
82-83	4.0273936411366727E-4
84-85	0.0
86-87	0.0
88-89	5.7534194873381045E-5
90-91	8.054787282273347E-5
92-93	5.408214318097819E-4
94-95	5.408214318097819E-4
96-97	0.0
98-99	2.3013677949352416E-5
100	0.0010586291856702111
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4345242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.39516942351229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23357882947107	53.27122059837461
2	12.608466554459902	16.74282546108107
3	3.391884522673721	6.756142426437324
4	1.3696930758378567	3.6376401531386464
5	0.6718042506432484	2.2302278520447096
6	0.4126571041924279	1.6439063008003327
7	0.2521047931447351	1.1716978317326947
8	0.19603026667559764	1.0412370214450082
9	0.13597517290323122	0.8125285178071272
>10	0.6842617184110313	7.7572121366327975
>50	0.02965824144337856	1.3310170835057078
>100	0.012673518230380121	1.5225985204217276
>500	6.925441534691013E-4	0.3071569553012946
>1k	3.4627183993543456E-4	0.42744453489118456
>5k	6.925436798696775E-5	0.2910632428849622
>10k+	1.0388155198045164E-4	1.0560813635008484
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18297	0.42108126543930113	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16980	0.39077225158000406	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10658	0.24527977958419808	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7560	0.1739834052971043	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5100	0.11736975754169734	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4667	0.10740483498962773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.3013677949352416E-5	0.0	0.0	0.0
6	0.0	2.3013677949352416E-5	0.0	0.0	0.0
7	0.0	2.3013677949352416E-5	0.0	0.0	0.0
8	0.0	2.3013677949352416E-5	0.0	2.3013677949352416E-5	0.0
9	0.0	2.3013677949352416E-5	0.0	2.3013677949352416E-5	0.0
10-11	0.0	2.3013677949352416E-5	0.0	2.3013677949352416E-5	0.0
12-13	0.0	8.054787282273345E-5	0.0	4.602735589870483E-5	0.0
14-15	0.0	1.8410942359481933E-4	0.0	4.602735589870483E-5	0.0
16-17	0.0	2.646572964175528E-4	0.0	5.7534194873381045E-5	0.0
18-19	0.0	2.76164135392229E-4	0.0	6.904103384805726E-5	0.0
20-21	0.0	2.76164135392229E-4	0.0	1.0356155077208588E-4	0.0
22-23	0.0	2.76164135392229E-4	0.0	2.76164135392229E-4	0.0
24-25	0.0	2.9917781334158145E-4	0.0	8.515060841260394E-4	0.0
26-27	0.0	3.2219149129093387E-4	0.0	0.0017145190072267553	0.0
28-29	0.0	3.2219149129093387E-4	0.0	0.004257530420630197	0.0
30-31	0.0	3.2219149129093387E-4	0.0	0.011288209034157361	0.0
32-33	0.0	3.2219149129093387E-4	0.0	0.027110112624337147	0.0
34-35	0.0	3.2219149129093387E-4	0.0	0.05267830882606769	0.0
36-37	0.0	3.336983302656101E-4	0.0	0.09203169811946033	0.0
38-39	0.0	3.567120082149625E-4	0.0	0.14580315664812224	0.0
40-41	0.0	3.912325251389911E-4	0.0	0.20804364866214586	0.0
42-43	0.0	4.142462030883435E-4	0.0	0.27435756167320485	0.0
44-45	0.0	4.257530420630197E-4	0.0	0.3510276297614724	0.0
46-47	0.0	4.3725988103769593E-4	0.0	0.4398374129680234	0.0
48-49	0.0	4.3725988103769593E-4	0.0	0.5372773254055816	0.0
50-51	0.0	4.3725988103769593E-4	0.0	0.6342454574451779	0.0
52-53	0.0	5.293145928351056E-4	0.0	0.7320766024078751	0.0
54-55	0.0	5.293145928351056E-4	0.0	0.8441532140212213	0.0
56-57	0.0	5.408214318097819E-4	0.0	0.9749629594853404	0.0
58-59	0.0	5.52328270784458E-4	0.0	1.114609957282011	0.0
60-61	0.0	5.52328270784458E-4	0.0	1.2537506541637957	0.0
62-63	0.0	5.52328270784458E-4	0.0	1.3984031269144503	0.0
64-65	0.0	5.638351097591342E-4	0.0	1.5583136681455256	0.0
66-67	0.0	5.983556266831629E-4	0.0	1.7410767915803078	0.0
68-69	0.0	6.213693046325153E-4	0.0	1.9376711354626508	0.0
70-71	0.0	6.213693046325153E-4	0.0	2.1522276549844634	0.0
72-73	0.0	6.904103384805726E-4	0.0	2.382387448155937	0.0
74-75	0.0	7.13424016429925E-4	0.0	2.6104645034729943	0.0
76-77	0.0	7.13424016429925E-4	0.0	2.852315245042739	0.0
78-79	0.0	7.13424016429925E-4	0.0	3.121437194982466	0.0
80-81	0.0	7.13424016429925E-4	0.0	3.4139410417187355	0.0
82-83	2.3013677949352416E-5	7.13424016429925E-4	0.0	3.7126240609844054	0.0
84-85	2.3013677949352416E-5	7.364376943792773E-4	0.0	4.014908720849149	0.0
86-87	2.3013677949352416E-5	7.479445333539536E-4	0.0	4.339021854248854	0.0
88	2.3013677949352416E-5	7.594513723286298E-4	0.0	4.598201895314461	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9160	0.0	57.96774	1
GTATCAA	16295	0.0	40.067738	1
GTGGTAT	3585	0.0	33.089035	1
ATCAACG	20085	0.0	32.396114	3
TCAACGC	20300	0.0	32.07618	4
TATCAAC	20745	0.0	31.388113	2
CAACGCA	21015	0.0	31.153315	5
TGGTATC	3790	0.0	30.041298	2
AACGCAG	22000	0.0	29.864624	6
TATAACG	485	0.0	29.10195	2
ATAACGC	480	0.0	28.424925	3
ACGCAGA	24745	0.0	26.51371	7
CGCAGAG	24640	0.0	26.512255	8
GTACCTG	3350	0.0	24.871458	1
GCAGAGT	26630	0.0	24.019255	9
TACCTGG	3350	0.0	24.015623	2
TAACGCA	685	0.0	21.978699	4
GTACATG	10190	0.0	21.804222	1
CAGAGTA	26170	0.0	21.76564	10-11
GAGTACT	22030	0.0	21.141302	12-13
>>END_MODULE