FastQCFastQC Report
Wed 25 May 2016
SRR2049511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6067761
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54950.041.263921
GTATCAA87650.035.8551031
TCAACGC88900.033.5601844
ATCAACG89550.033.316593
CAACGCA94800.031.5255055
TATCAAC102450.029.8208452
AACGCAG101750.029.4219156
ACGCAGA119250.024.931887
CGCAGAG125100.023.432588
GTACATG135300.022.3575481
GTGGTAT35750.021.4758191
TACATGG137200.020.999642
TCTGTCG20200.019.9315538
ACATGGG139750.019.5911313
TGGTATC36150.019.2525442
GCAGAGT146100.019.0116869
GTCTCGC19450.017.8946721
AGAGTAC128250.016.89587210-11
GAGTACA107200.016.4724731
TCGCTCT21300.016.3375474
Produced by FastQC (version 0.11.5)