##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049511_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6067761 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.988423571726045 31.0 31.0 34.0 28.0 34.0 2 31.071274395942755 31.0 31.0 34.0 28.0 34.0 3 31.23077952477034 31.0 31.0 34.0 28.0 34.0 4 34.80081367740094 37.0 35.0 37.0 32.0 37.0 5 34.8167531648 37.0 35.0 37.0 32.0 37.0 6 34.83191427612261 37.0 35.0 37.0 32.0 37.0 7 30.813583461840373 37.0 33.0 37.0 2.0 37.0 8 32.6059683299985 37.0 33.0 37.0 17.0 37.0 9 35.42328974394344 39.0 34.0 39.0 29.0 39.0 10-11 35.98912473645551 39.0 36.0 39.0 31.5 39.0 12-13 36.163132743692444 39.0 37.0 39.0 32.0 39.0 14-15 37.37171462422465 40.0 38.0 41.0 32.0 41.0 16-17 37.386892875312654 40.0 38.0 41.0 32.0 41.0 18-19 37.38648086172148 40.0 38.0 41.0 31.5 41.0 20-21 37.461915441297045 40.0 38.0 41.0 32.0 41.0 22-23 37.412133239921616 40.0 38.0 41.0 31.5 41.0 24-25 37.386186024795634 40.0 38.0 41.0 31.0 41.0 26-27 37.4091077417189 40.0 38.0 41.0 31.0 41.0 28-29 37.383483957261994 40.0 38.0 41.0 31.0 41.0 30-31 37.29788805129273 40.0 37.0 41.0 31.0 41.0 32-33 37.23457697163748 40.0 37.0 41.0 31.0 41.0 34-35 37.165880627137426 40.0 37.0 41.0 31.0 41.0 36-37 36.9779520815009 40.0 37.0 41.0 30.0 41.0 38-39 36.84982393011195 40.0 37.0 41.0 30.0 41.0 40-41 36.76625026925088 40.0 37.0 41.0 30.0 41.0 42-43 36.64210769013479 40.0 36.5 41.0 30.0 41.0 44-45 36.51859804629747 40.0 36.0 41.0 30.0 41.0 46-47 36.35620049965712 40.0 36.0 41.0 30.0 41.0 48-49 36.271030945352 40.0 36.0 41.0 29.5 41.0 50-51 36.1348354030424 40.0 35.5 41.0 29.0 41.0 52-53 36.00985841400147 39.0 35.0 41.0 29.0 41.0 54-55 35.882758566133376 39.0 35.0 41.0 29.0 41.0 56-57 35.68512495795402 39.0 35.0 41.0 28.0 41.0 58-59 35.505149593070655 39.0 35.0 41.0 28.0 41.0 60-61 35.27717621046709 39.0 35.0 40.5 28.0 41.0 62-63 35.028147614911006 38.0 34.0 40.0 28.0 41.0 64-65 34.65763672959433 37.5 34.0 40.0 26.5 41.0 66-67 31.15973280753807 36.0 31.0 40.0 6.5 41.0 68-69 31.51052348634035 36.0 30.5 39.0 11.0 41.0 70-71 31.653449188259064 35.5 30.0 39.0 15.5 41.0 72-73 32.88135359978747 35.0 32.0 39.0 24.0 41.0 74-75 32.957173824084364 35.0 33.0 38.5 25.0 40.0 76-77 32.641844001436446 35.0 33.0 37.0 24.5 39.5 78-79 29.24942528224167 35.0 29.0 36.5 6.5 39.0 80-81 30.999493635296446 35.0 30.0 36.0 18.5 39.0 82-83 31.372312455945448 35.0 32.0 36.0 20.0 37.5 84-85 29.50089769521245 35.0 30.0 35.5 10.5 37.0 86-87 29.081072243946323 34.5 28.0 35.0 10.0 37.0 88-89 30.25894675152828 34.0 30.5 35.0 9.0 36.0 90-91 30.34622342574139 35.0 31.5 35.0 4.0 36.0 92-93 30.126028035712018 35.0 32.0 35.0 2.0 36.0 94-95 29.84892541087231 35.0 31.0 35.0 2.0 36.0 96-97 29.58260707697617 35.0 31.0 35.0 2.0 35.0 98-99 29.325678120809307 35.0 31.0 35.0 2.0 35.0 100 29.081614453832312 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.26277380297547026 1101 2 0.29182985183388155 1101 3 0.30837892566580294 1101 4 0.2369913505139536 1101 5 0.2766944737270336 1101 6 0.2659022886078972 1101 7 -12.101692186379111 1101 8 -6.526870349592908 1101 9 -2.212976797864279 1101 10-11 -0.1815625014245299 1101 12-13 0.315723471966983 1101 14-15 0.529420919349235 1101 16-17 0.5758804946728802 1101 18-19 0.5716666554656982 1101 20-21 0.532153209945939 1101 22-23 0.5293714879552525 1101 24-25 0.5580381555301912 1101 26-27 0.5393126364629808 1101 28-29 0.5185822409574854 1101 30-31 0.587166109386196 1101 32-33 0.5664008018089959 1101 34-35 0.5969853387133952 1101 36-37 0.6435838606341377 1101 38-39 0.6634683685678908 1101 40-41 0.6065245332980638 1101 42-43 0.542917424462722 1101 44-45 0.5243955573579981 1101 46-47 0.5225900348439367 1101 48-49 0.46603803325958637 1101 50-51 0.5339608608322237 1101 52-53 0.5374906272646811 1101 54-55 0.4476873854261143 1101 56-57 0.48650655185424796 1101 58-59 0.401748601383737 1101 60-61 0.37594880672416053 1101 62-63 0.33537031185657895 1101 64-65 0.27625382758227346 1101 66-67 -10.235446896878788 1101 68-69 -8.101735359256402 1101 70-71 -6.63611163871019 1101 72-73 -1.5080200657468765 1101 74-75 -0.2473796610680239 1101 76-77 0.011374718841572928 1101 78-79 3.1353875972048897 1101 80-81 1.0193549509336108 1101 82-83 0.34932207197624976 1101 84-85 -5.337867525765702 1101 86-87 -5.8517397257334025 1101 88-89 -1.3735921960328739 1101 90-91 -0.05628071391828726 1101 92-93 0.2988585638407244 1101 94-95 0.3703415799610852 1101 96-97 0.5073338857777117 1101 98-99 0.5184819587117531 1101 100 0.5581750373813534 1102 1 0.3196609513256483 1102 2 0.32691673682050393 1102 3 0.35262170280008576 1102 4 0.32735305814418325 1102 5 0.3616222457065916 1102 6 0.3664363450567123 1102 7 -12.35152499129779 1102 8 -6.631301290206839 1102 9 -2.1841905349681525 1102 10-11 -0.10561773849686773 1102 12-13 0.42788641218711376 1102 14-15 0.5950377832423825 1102 16-17 0.6310063535948984 1102 18-19 0.6315563750531012 1102 20-21 0.6178268475808721 1102 22-23 0.6477180639389388 1102 24-25 0.6949460739056619 1102 26-27 0.6292670072676998 1102 28-29 0.6612278094862702 1102 30-31 0.6373671877714742 1102 32-33 0.6818225356782364 1102 34-35 0.6676257593344275 1102 36-37 0.79291333428651 1102 38-39 0.7391135839587051 1102 40-41 0.7067522010284861 1102 42-43 0.7285876648532152 1102 44-45 0.6866254972945498 1102 46-47 0.7188454083550511 1102 48-49 0.6577294189711225 1102 50-51 0.739110711289797 1102 52-53 0.7488363526213675 1102 54-55 0.6853525511455629 1102 56-57 0.7320975695229421 1102 58-59 0.6886232986419927 1102 60-61 0.6569734730959382 1102 62-63 0.6419756912523766 1102 64-65 0.6406138341282954 1102 66-67 -10.237930465096465 1102 68-69 -6.547245227919017 1102 70-71 -6.116342725191981 1102 72-73 -0.7637933225876452 1102 74-75 0.2082144204878631 1102 76-77 0.37932032518008896 1102 78-79 3.538477294108155 1102 80-81 1.4199468666577175 1102 82-83 0.7378558710777874 1102 84-85 -5.246371059253661 1102 86-87 -5.766349102190336 1102 88-89 -1.0676650982103766 1102 90-91 0.267884443325741 1102 92-93 0.6486441157916367 1102 94-95 0.6568882400877634 1102 96-97 0.6860140799387899 1102 98-99 0.6791688266399944 1102 100 0.7798641976926071 1103 1 0.35594014060738033 1103 2 0.2942624740540971 1103 3 0.3573878801858612 1103 4 0.34084138270168296 1103 5 0.32453867153355986 1103 6 0.3468539953749641 1103 7 -12.14825753315256 1103 8 -6.559070091121342 1103 9 -2.1950852819461346 1103 10-11 -0.1468934061207321 1103 12-13 0.3570275620045251 1103 14-15 0.5400490628825452 1103 16-17 0.49754194402261476 1103 18-19 0.5938415638861727 1103 20-21 0.5816616942217436 1103 22-23 0.5356663677440849 1103 24-25 0.6088440955830237 1103 26-27 0.5660018066041346 1103 28-29 0.5915818119262681 1103 30-31 0.5602296610027224 1103 32-33 0.6341335193802564 1103 34-35 0.5850490932781724 1103 36-37 0.6555712102394438 1103 38-39 0.6632529663611777 1103 40-41 0.634266187518854 1103 42-43 0.6366391130087763 1103 44-45 0.5678003834258121 1103 46-47 0.6251233554139262 1103 48-49 0.6330926543219633 1103 50-51 0.6445782751219156 1103 52-53 0.6002352786085936 1103 54-55 0.5959240837614814 1103 56-57 0.6598785224706702 1103 58-59 0.6424739564358077 1103 60-61 0.5827981716069601 1103 62-63 0.49681256185861855 1103 64-65 0.4164878331476203 1103 66-67 -10.209603231309245 1103 68-69 -8.101354536910097 1103 70-71 -6.579095946308254 1103 72-73 -1.328569441809151 1103 74-75 -0.1500512080975085 1103 76-77 0.19925446237594002 1103 78-79 3.2948097294249195 1103 80-81 1.1361693311400458 1103 82-83 0.4496791847028483 1103 84-85 -5.348037394143027 1103 86-87 -5.786549381747896 1103 88-89 -1.2782258162597628 1103 90-91 0.03940829815872604 1103 92-93 0.457865178568337 1103 94-95 0.5323882347851807 1103 96-97 0.551464802498419 1103 98-99 0.537567357534158 1103 100 0.6327797973974718 1104 1 0.13380006740347739 1104 2 0.18582759540645455 1104 3 0.17985776648553653 1104 4 0.1599224698678796 1104 5 0.21245881051753202 1104 6 0.22078750335102626 1104 7 -12.295662080557108 1104 8 -6.7226780760059235 1104 9 -2.457743076518824 1104 10-11 -0.3401316291461782 1104 12-13 0.22396897149912576 1104 14-15 0.4264375790583017 1104 16-17 0.4160309497456609 1104 18-19 0.42542786142991673 1104 20-21 0.41969699395917104 1104 22-23 0.46044646699227343 1104 24-25 0.4809554166305148 1104 26-27 0.4675519890053721 1104 28-29 0.4199250487801436 1104 30-31 0.333627983337216 1104 32-33 0.3235627662563161 1104 34-35 0.37362764143119165 1104 36-37 0.39782714398474184 1104 38-39 0.3997222624049357 1104 40-41 0.39560077438535046 1104 42-43 0.3501371094855017 1104 44-45 0.3449942946622713 1104 46-47 0.37995082726268237 1104 48-49 0.29016279437082204 1104 50-51 0.29992632834230903 1104 52-53 0.274470608163071 1104 54-55 0.285696835236358 1104 56-57 0.25607502292248796 1104 58-59 0.21988541044300547 1104 60-61 0.170528150826307 1104 62-63 0.11129938911140158 1104 64-65 0.04724746816257408 1104 66-67 -10.490294865192368 1104 68-69 -8.413076593313004 1104 70-71 -6.915501957182347 1104 72-73 -1.8182481782964004 1104 74-75 -0.5725782944543738 1104 76-77 -0.24682870285616332 1104 78-79 2.8577498732161217 1104 80-81 0.7505283710309989 1104 82-83 0.09526245794569732 1104 84-85 -5.628956915488079 1104 86-87 -6.157391956845789 1104 88-89 -1.573954067307163 1104 90-91 -0.29200092261219623 1104 92-93 0.04115180866347856 1104 94-95 0.08549666236169173 1104 96-97 0.23278807105572596 1104 98-99 0.22277962146982588 1104 100 0.28688861746373817 1105 1 0.08152043962598654 1105 2 0.05827121949901937 1105 3 0.09279836601992386 1105 4 0.09865468010114142 1105 5 0.05852690483535383 1105 6 0.14055334672708142 1105 7 -12.194122626595316 1105 8 -6.71091658205583 1105 9 -2.5110984529749416 1105 10-11 -0.4459832979910914 1105 12-13 0.05918146122555612 1105 14-15 0.2359978079364211 1105 16-17 0.293591692609219 1105 18-19 0.22803486129964057 1105 20-21 0.2646326221581816 1105 22-23 0.2746872187735292 1105 24-25 0.27173840120395454 1105 26-27 0.2696358830141037 1105 28-29 0.28389109252623257 1105 30-31 0.33072596799537735 1105 32-33 0.2871586417401204 1105 34-35 0.35683530180909884 1105 36-37 0.41366714429793205 1105 38-39 0.3450678022191269 1105 40-41 0.37458186957356787 1105 42-43 0.31901593586015053 1105 44-45 0.20342172265278435 1105 46-47 0.22301315284703804 1105 48-49 0.15342716395996803 1105 50-51 0.2387120454769871 1105 52-53 0.19039490163913086 1105 54-55 0.20209436212655163 1105 56-57 0.1923103753380886 1105 58-59 0.18087076880278374 1105 60-61 0.13737703769884746 1105 62-63 0.033156733291143325 1105 64-65 -0.08307820003750521 1105 66-67 -10.375239834750037 1105 68-69 -8.39361200694579 1105 70-71 -6.8538167599716715 1105 72-73 -1.8580292534658085 1105 74-75 -0.6147058246098425 1105 76-77 -0.3674219189042205 1105 78-79 2.77959427549591 1105 80-81 0.6530766436009259 1105 82-83 0.0068485280576062735 1105 84-85 -5.522570345948502 1105 86-87 -6.00991651943108 1105 88-89 -1.6456810692236488 1105 90-91 -0.3709451658026168 1105 92-93 -0.02932978474424175 1105 94-95 0.061473335372507876 1105 96-97 0.13182819103287713 1105 98-99 0.13343889943374165 1105 100 0.20270451317656324 1106 1 0.24726452622313033 1106 2 0.28184963992862677 1106 3 0.25763540037466015 1106 4 0.2646792873122976 1106 5 0.228274807462995 1106 6 0.2785700406728182 1106 7 -12.247914886678512 1106 8 -6.694570146931419 1106 9 -2.4598250599814833 1106 10-11 -0.27843653983669014 1106 12-13 0.2766758426554716 1106 14-15 0.4596797845367604 1106 16-17 0.4926699671100465 1106 18-19 0.4453071764067644 1106 20-21 0.5191525814823947 1106 22-23 0.48161363419634995 1106 24-25 0.555022070683755 1106 26-27 0.5158176271169239 1106 28-29 0.5153477177985906 1106 30-31 0.5504085073884752 1106 32-33 0.5164510366190953 1106 34-35 0.5440988033647471 1106 36-37 0.5719154532803756 1106 38-39 0.5367699069623058 1106 40-41 0.5443091737367567 1106 42-43 0.5681095254452586 1106 44-45 0.4802535739683904 1106 46-47 0.5888852798351252 1106 48-49 0.5618807197851865 1106 50-51 0.5395543403220415 1106 52-53 0.5695233101871295 1106 54-55 0.5714442885047362 1106 56-57 0.5747264823631539 1106 58-59 0.5273167862858301 1106 60-61 0.4718251814424903 1106 62-63 0.3980840661794147 1106 64-65 0.2734269941826213 1106 66-67 -10.314049774722012 1106 68-69 -8.266628142081696 1106 70-71 -6.66528419146 1106 72-73 -1.493458050872512 1106 74-75 -0.25375643670454906 1106 76-77 0.062072056602779924 1106 78-79 3.151998329120154 1106 80-81 1.0810763126818586 1106 82-83 0.3885666506562089 1106 84-85 -5.379999333911993 1106 86-87 -5.877461482867783 1106 88-89 -1.340734935227914 1106 90-91 -0.10335234362727519 1106 92-93 0.2158853671167087 1106 94-95 0.34363696784657094 1106 96-97 0.4816074707483615 1106 98-99 0.4683623434470938 1106 100 0.5323946455691448 1107 1 0.10217774980443295 1107 2 0.15046440452266907 1107 3 0.15424845686845856 1107 4 0.073943170742929 1107 5 0.09448099884948391 1107 6 0.06523145938683683 1107 7 -12.214944482205262 1107 8 -6.749692979951703 1107 9 -2.515491779306707 1107 10-11 -0.4895060498676784 1107 12-13 0.11410102877227501 1107 14-15 0.24683494663682382 1107 16-17 0.2881541898234943 1107 18-19 0.30785344401419934 1107 20-21 0.2563130889194696 1107 22-23 0.2725820657332605 1107 24-25 0.36152481784899493 1107 26-27 0.2876501341184081 1107 28-29 0.28405490417245005 1107 30-31 0.28928302989639576 1107 32-33 0.2979253370536128 1107 34-35 0.2749116015600137 1107 36-37 0.3834940829332041 1107 38-39 0.37834491734276554 1107 40-41 0.40923912829236286 1107 42-43 0.397785732292256 1107 44-45 0.30076402101173016 1107 46-47 0.35895577022268554 1107 48-49 0.3527223702993254 1107 50-51 0.38403736219824225 1107 52-53 0.32274094147453525 1107 54-55 0.35507949177848275 1107 56-57 0.33202811086440676 1107 58-59 0.34515510884730816 1107 60-61 0.2605019588909059 1107 62-63 0.16239312129606986 1107 64-65 0.012099556014440793 1107 66-67 -10.320893519200734 1107 68-69 -8.307017071025246 1107 70-71 -6.836746594642033 1107 72-73 -1.8041701062488116 1107 74-75 -0.5420853736968922 1107 76-77 -0.3030692575324636 1107 78-79 2.8239601821653793 1107 80-81 0.742658002765836 1107 82-83 0.10260292690508166 1107 84-85 -5.537121302140552 1107 86-87 -5.980514163398034 1107 88-89 -1.514125375311437 1107 90-91 -0.2790006156365443 1107 92-93 0.09520255436842362 1107 94-95 0.16176343856662712 1107 96-97 0.17151626388099572 1107 98-99 0.20706619251570402 1107 100 0.2704572278211259 1108 1 -0.08852747036196362 1108 2 -0.056346852829349814 1108 3 -0.04596779432270637 1108 4 -0.12231117879328224 1108 5 -0.12582070002818568 1108 6 -0.10461254696243572 1108 7 -12.406802394418847 1108 8 -6.927656323883724 1108 9 -2.8591319564826705 1108 10-11 -0.7214505438891408 1108 12-13 -0.1512022108838309 1108 14-15 -0.05148993124179668 1108 16-17 0.005004465921054191 1108 18-19 -0.028777174009654516 1108 20-21 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2216 2 -0.67596227920723 2216 3 -0.8291926516218986 2216 4 -0.890395272736292 2216 5 -0.6601454340543143 2216 6 -0.952390225383688 2216 7 3.2106225399526203 2216 8 1.3011363453080165 2216 9 0.08126370958572693 2216 10-11 -0.676817712377229 2216 12-13 -1.0195587723532356 2216 14-15 -1.2606544528720676 2216 16-17 -1.3826503971904955 2216 18-19 -1.8493880995369736 2216 20-21 -1.4613682676679005 2216 22-23 -1.2780484108643009 2216 24-25 -1.282654850171241 2216 26-27 -1.6382621989968982 2216 28-29 -1.8435869108236034 2216 30-31 -1.802126961866847 2216 32-33 -2.6671488615415058 2216 34-35 -1.7403835065132327 2216 36-37 -1.430314868678714 2216 38-39 -1.6769976204086632 2216 40-41 -1.6432584573934648 2216 42-43 -1.2783156566351934 2216 44-45 -1.3952231273254156 2216 46-47 -1.7097119523499629 2216 48-49 -1.756916796537915 2216 50-51 -2.1915401953444302 2216 52-53 -1.962409589696108 2216 54-55 -1.5638941551240606 2216 56-57 -1.6158916618037296 2216 58-59 -1.9114218015063642 2216 60-61 -2.077334632663529 2216 62-63 -1.927896137970606 2216 64-65 -1.5715573503015605 2216 66-67 1.8471456207761143 2216 68-69 0.9708142732833984 2216 70-71 0.637925156558449 2216 72-73 -1.2268194402166372 2216 74-75 -1.9515722348129785 2216 76-77 -1.5530406430399495 2216 78-79 1.0548299572763469 2216 80-81 -0.47018101547834945 2216 82-83 -1.33541700617209 2216 84-85 0.4972897289561615 2216 86-87 0.53172731881331 2216 88-89 -0.7026814507848229 2216 90-91 -1.0531478467243431 2216 92-93 -1.0908300005086637 2216 94-95 -1.1460286245759477 2216 96-97 -1.1900415267401243 2216 98-99 -1.170809596266082 2216 100 -2.261899403240463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78501.0 3 15960.0 4 9232.0 5 8482.0 6 8019.0 7 9824.0 8 11660.0 9 13089.0 10 14711.0 11 16486.0 12 18574.0 13 20893.0 14 23823.0 15 24576.0 16 25740.0 17 26225.0 18 24561.0 19 23550.0 20 22238.0 21 22665.0 22 24240.0 23 27753.0 24 33471.0 25 41274.0 26 51420.0 27 62724.0 28 79406.0 29 101729.0 30 125788.0 31 154520.0 32 205016.0 33 285985.0 34 428747.0 35 591266.0 36 653410.0 37 1100180.0 38 1248610.0 39 432419.0 40 994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69250714656129 20.5407221298945 13.781959247375033 22.984811476169174 2 16.299887929329554 20.65256444063551 39.420479266925966 23.62706836310897 3 18.409224215973122 25.14006586700916 30.404729582850514 26.045980334167208 4 13.004909335186227 17.4723417271736 38.46551796881283 31.057230968827337 5 12.895676511121446 37.1552163722142 34.998729326002206 14.950377790662145 6 30.340521557751142 37.838350982510796 18.08728646121411 13.733840998523943 7 26.789473487969754 32.23621266298532 22.61839156745551 18.355922281589418 8 24.4207911980698 35.790753095046604 21.24878043956913 18.53967526731446 9 24.15039089377449 17.652096053222927 22.281398360944014 35.91611469205857 10-11 23.337059584251918 27.18673329420852 28.973281907444935 20.50292521409462 12-13 24.109988841023895 24.700511440710997 28.865647476886448 22.323852241378656 14-15 21.734376156213138 27.309010687797358 26.07343136949527 24.883181786494227 16-17 21.052367889553697 30.344716200169913 26.571988440516204 22.030927469760183 18-19 20.878731050876922 29.149631964739548 28.696969112659517 21.274667871724017 20-21 22.284546144780588 28.749846936950878 27.912668280771115 21.052938637497423 22-23 21.863214607761794 28.890881569897164 27.89399812336034 21.3519056989807 24-25 21.599655952170828 28.73464363543653 28.113145853964916 21.55255455842773 26-27 21.419158666721604 28.45231758065181 28.2433027069342 21.88522104569239 28-29 21.486821910091713 28.57523557701103 28.286381088510243 21.65156142438702 30-31 21.97798512317683 29.201927136049616 27.625991500488524 21.194096240285027 32-33 21.240952180046673 28.901497422442553 28.264870396983397 21.592680000527377 34-35 21.258837076423106 28.2883103959798 28.438077385736644 22.014775141860447 36-37 22.041028656543542 28.901526906391634 27.622174842474788 21.43526959459003 38-39 21.822290568890427 28.898642810302782 27.858629520388007 21.420437100418788 40-41 21.444499874006244 28.597377187400756 28.19346378342852 21.764659155164484 42-43 21.337142647133593 28.520543703157646 28.191138755581857 21.9511748941269 44-45 21.661274772362077 28.69679864859297 28.066326068147173 21.57560051089778 46-47 21.415604037284357 29.134839974174696 27.79780781573885 21.651748172802098 48-49 21.198506687234534 28.537699719869952 28.30250419535847 21.961289397537048 50-51 21.26066233237784 28.28055887044581 28.31204506324349 22.14673373393285 52-53 21.225113400794378 28.38204319114413 28.225354099630202 22.16748930843129 54-55 21.380565200599 28.887116857933666 27.974915534854457 21.757402406612876 56-57 21.004767892782567 28.602626708439978 28.051459621238468 22.34114577753899 58-59 20.82161081747254 28.067926125365645 28.487036110155696 22.623426947006127 60-61 21.343744342462386 28.53696376019861 27.915614198146095 22.203677699192912 62-63 21.218600960969553 28.505261204471864 27.94480275798328 22.331335076575304 64-65 21.152741471007015 28.116755268326116 28.240764355458875 22.489738905207997 66-67 21.08130709989398 28.246011973701247 27.8702155056282 22.802465420776578 68-69 21.839268188801096 28.36506579553424 27.665342496806065 22.130323518858592 70-71 21.623581753658485 28.12602568819738 27.83454997859523 22.415842579548904 72-73 21.284035059875468 28.238904279182957 28.21951465115892 22.257546009782654 74-75 21.390569548831596 28.230579457267563 27.97978659285772 22.399064401043123 76-77 21.45439151702052 28.388650716992043 27.627467563723584 22.529490202263855 78-79 21.733106139793033 28.515150461701428 27.651647723851987 22.100095674653552 80-81 21.460364649460683 28.220353432215727 27.84716495505891 22.472116963264675 82-83 21.443141563330432 28.228215458865304 27.913008931840483 22.415634045963788 84-85 21.682759542509256 28.134646726377433 27.688727353141886 22.49386637797143 86-87 21.868675331832883 28.283883080906968 27.629034767463175 22.218406819796975 88-89 21.679268637676078 28.087374398149002 27.714560309365982 22.518796654808945 90-91 21.75611460488328 27.914563433218635 27.646703751972073 22.682618209926012 92-93 22.065992985423918 28.132321528680183 27.322829461219545 22.478856024676354 94-95 22.114488523780416 27.830904799627326 27.478666395604172 22.575940280988082 96-97 22.0741320631886 27.785218086377533 27.579558470881988 22.56109137955188 98-99 22.196183221644812 27.829218950572603 27.386945088208787 22.587652739573798 100 22.518822904659075 27.894999161082396 27.152581806003358 22.433596128255175 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3483.0 1 2594.5 2 2131.5 3 2500.5 4 2271.5 5 1941.5 6 1728.5 7 1719.5 8 1762.0 9 1676.0 10 1514.5 11 1350.5 12 1335.5 13 1411.5 14 1523.0 15 1698.5 16 1977.5 17 2459.0 18 3205.0 19 4343.0 20 6063.5 21 8389.0 22 11885.5 23 17038.5 24 23931.5 25 32517.5 26 43497.0 27 57470.5 28 74133.5 29 92175.0 30 110142.5 31 126129.5 32 140256.5 33 154807.0 34 167943.0 35 179717.0 36 190497.0 37 199485.5 38 206754.5 39 210866.0 40 213115.5 41 213566.0 42 211211.5 43 208806.0 44 206425.0 45 203558.0 46 200704.0 47 197192.0 48 193531.0 49 190852.5 50 187025.5 51 180124.5 52 172783.5 53 166486.5 54 160857.0 55 154108.5 56 143833.0 57 130394.0 58 115989.5 59 102357.0 60 87814.0 61 72047.0 62 58508.5 63 47135.0 64 37215.5 65 29013.5 66 22331.0 67 16939.5 68 12755.0 69 9543.5 70 6913.5 71 5270.5 72 4133.5 73 3028.5 74 2171.5 75 1521.0 76 1056.5 77 766.0 78 549.5 79 370.5 80 249.0 81 175.5 82 136.5 83 103.5 84 84.5 85 74.0 86 61.0 87 50.5 88 37.0 89 24.0 90 18.0 91 16.5 92 14.5 93 10.5 94 11.5 95 11.0 96 12.5 97 13.0 98 13.5 99 16.0 100 23.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.019892016181916196 2 0.002653367527165292 3 0.0013184434917591513 4 0.002257834479637547 5 0.00189526251940378 6 0.004037733193512401 7 11.59869348842184 8 1.6480543646989393E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.636886983518303E-4 18-19 0.0 20-21 0.0 22-23 8.240271823494697E-6 24-25 0.0 26-27 1.8128598011688332E-4 28-29 0.0 30-31 8.240271823494697E-6 32-33 1.6480543646989393E-5 34-35 3.2961087293978786E-5 36-37 3.2961087293978786E-5 38-39 3.2961087293978786E-5 40-41 0.0 42-43 2.4720815470484088E-5 44-45 1.8128598011688332E-4 46-47 0.0011289172398187735 48-49 7.169036486440386E-4 50-51 3.6257196023376664E-4 52-53 2.0600679558736742E-4 54-55 3.543316884102719E-4 56-57 0.0014997294718760346 58-59 3.6257196023376664E-4 60-61 0.0013431643072296354 62-63 0.0022907955669315256 64-65 9.970728906428582E-4 66-67 5.799519789919215 68-69 5.450239388136744 70-71 0.0031065824774575007 72-73 0.001384365666347109 74-75 0.003362030903985836 76-77 0.006007158159327633 78-79 5.42278280242086 80-81 0.005232572607919131 82-83 0.005117208802390206 84-85 5.799989485413153 86-87 0.004103655368100359 88-89 0.0020683082276971685 90-91 0.003600998786867182 92-93 0.004161337270864821 94-95 0.004606311949333535 96-97 0.0034773947095147616 98-99 0.006040119246621612 100 0.006905347788088555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6067761.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.95993171613148 #Duplication Level Percentage of deduplicated Percentage of total 1 93.50604700080589 85.05303650238685 2 4.862107926512291 8.84514009984039 3 0.9342413462075592 2.54935587172279 4 0.33111953371917346 1.2047444070789326 5 0.15148853672765056 0.6889693478261886 6 0.07661455767191451 0.4181312960579377 7 0.04760897783330977 0.3031356560954872 8 0.028054227370657873 0.20414484847870937 9 0.018240965935481104 0.14932773143348774 >10 0.044242682971083934 0.5581386881884524 >50 1.744170952138065E-4 0.00954620633244006 >100 4.120251698845656E-5 0.005148315405755994 >500 1.862463278268475E-5 0.01118102915259679 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.6480543646989393E-5 0.0 0.0 0.0 6 1.6480543646989393E-5 1.6480543646989393E-5 0.0 0.0 0.0 7 1.6480543646989393E-5 3.2961087293978786E-5 0.0 0.0 0.0 8 1.6480543646989393E-5 3.2961087293978786E-5 0.0 0.0 0.0 9 1.6480543646989393E-5 3.2961087293978786E-5 0.0 0.0 0.0 10-11 1.6480543646989393E-5 3.2961087293978786E-5 0.0 0.0 0.0 12-13 1.6480543646989393E-5 4.1201359117473484E-5 0.0 4.1201359117473484E-5 0.0 14-15 1.6480543646989393E-5 5.7681902764462874E-5 0.0 6.592217458795757E-5 0.0 16-17 1.6480543646989393E-5 6.592217458795757E-5 0.0 9.064299005844166E-5 0.0 18-19 2.4720815470484088E-5 6.592217458795757E-5 0.0 1.6480543646989394E-4 0.0 20-21 3.2961087293978786E-5 6.592217458795757E-5 0.0 2.7192897017532495E-4 0.0 22-23 3.2961087293978786E-5 6.592217458795757E-5 0.0 7.498647359380174E-4 0.0 24-25 3.2961087293978786E-5 6.592217458795757E-5 0.0 0.00189526251940378 0.0 26-27 3.2961087293978786E-5 6.592217458795757E-5 0.0 0.003716362592396108 0.0 28-29 3.2961087293978786E-5 6.592217458795757E-5 0.0 0.010390982769426812 0.0 30-31 3.2961087293978786E-5 8.240271823494696E-5 0.0 0.02956609530269897 0.0 32-33 3.2961087293978786E-5 8.240271823494696E-5 0.0 0.0758187410479747 0.0 34-35 3.2961087293978786E-5 8.240271823494696E-5 0.0 0.15787536786633488 0.0 36-37 3.2961087293978786E-5 9.064299005844166E-5 0.0 0.29122768678594957 0.0 38-39 3.2961087293978786E-5 1.1536380552892575E-4 0.0 0.4751835149736452 0.0 40-41 3.2961087293978786E-5 1.4832489282290453E-4 0.0 0.6873457936131631 0.0 42-43 3.2961087293978786E-5 1.8128598011688332E-4 0.0 0.9185266196213068 0.0 44-45 3.2961087293978786E-5 1.89526251940378E-4 0.0 1.189812848594399 0.0 46-47 3.2961087293978786E-5 2.224873392343568E-4 0.0 1.5129880692400377 0.0 48-49 3.2961087293978786E-5 2.307276110578515E-4 0.0 1.8642131751728521 0.0 50-51 3.2961087293978786E-5 2.9664978564580906E-4 0.0 2.2276256431326154 0.0 52-53 3.2961087293978786E-5 3.048900574693038E-4 0.0 2.5880139313331556 0.0 54-55 3.2961087293978786E-5 3.213706011162931E-4 0.0 2.9786440171259216 0.0 56-57 3.2961087293978786E-5 3.296108729397878E-4 0.0 3.389083716382369 0.0 58-59 3.2961087293978786E-5 3.296108729397878E-4 0.0 3.8253896288927662 0.0 60-61 3.2961087293978786E-5 3.296108729397878E-4 0.0 4.263335355496039 0.0 62-63 3.2961087293978786E-5 3.296108729397878E-4 0.0 4.6942521302338704 0.0 64-65 3.2961087293978786E-5 3.4609141658677726E-4 0.0 5.166329062730058 0.0 66-67 3.2961087293978786E-5 3.4609141658677726E-4 0.0 5.684559757709639 0.0 68-69 4.1201359117473484E-5 3.4609141658677726E-4 0.0 6.16918662419301 0.0 70-71 4.9441630940968176E-5 3.54331688410272E-4 0.0 6.731346208263641 0.0 72-73 4.9441630940968176E-5 3.79052503880756E-4 0.0 7.349317153394803 0.0 74-75 4.9441630940968176E-5 4.0377331935124007E-4 0.0 7.888189729292239 0.0 76-77 4.9441630940968176E-5 4.120135911747348E-4 0.0 8.426897499753203 0.0 78-79 4.9441630940968176E-5 4.202538629982295E-4 0.0 9.029005921624137 0.0 80-81 6.592217458795757E-5 4.2849413482172417E-4 0.0 9.726874542355905 0.0 82-83 8.240271823494696E-5 4.779357657626924E-4 0.0 10.433395448502338 0.0 84-85 8.240271823494696E-5 4.779357657626924E-4 0.0 11.11543450706117 0.0 86-87 8.240271823494696E-5 4.944163094096818E-4 0.0 11.89145386576696 0.0 88 9.888326188193635E-5 5.603384839976393E-4 0.0 12.52137320504219 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5495 0.0 41.26392 1 GTATCAA 8765 0.0 35.855103 1 TCAACGC 8890 0.0 33.560184 4 ATCAACG 8955 0.0 33.31659 3 CAACGCA 9480 0.0 31.525505 5 TATCAAC 10245 0.0 29.820845 2 AACGCAG 10175 0.0 29.421915 6 ACGCAGA 11925 0.0 24.93188 7 CGCAGAG 12510 0.0 23.43258 8 GTACATG 13530 0.0 22.357548 1 GTGGTAT 3575 0.0 21.475819 1 TACATGG 13720 0.0 20.99964 2 TCTGTCG 2020 0.0 19.931553 8 ACATGGG 13975 0.0 19.591131 3 TGGTATC 3615 0.0 19.252544 2 GCAGAGT 14610 0.0 19.011686 9 GTCTCGC 1945 0.0 17.894672 1 AGAGTAC 12825 0.0 16.895872 10-11 GAGTACA 10720 0.0 16.472473 1 TCGCTCT 2130 0.0 16.337547 4 >>END_MODULE