FastQCFastQC Report
Wed 25 May 2016
SRR2049511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6067761
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193300.31856890869630494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191630.3158166579072577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105510.17388621601938506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111550.058.0790141
GTATCAA200500.039.2168271
GTGGTAT45400.033.8089831
ATCAACG248500.031.2802963
TCAACGC250600.031.0932774
TGGTATC47300.030.7386342
TATCAAC256600.030.4212442
CAACGCA258350.030.180075
AACGCAG269150.029.125976
ACGCAGA304750.025.6306087
CGCAGAG307400.025.3179238
ATAACGC5850.024.9340633
TATAACG6300.024.6468052
GCAGAGT332200.023.1024559
GTACATG138100.021.7860031
GAGTACT257100.021.30501212-13
CAGAGTA329900.020.75624810-11
TACATGG139450.020.413812
AGAGTAC312550.020.29200610-11
TAACGCA7150.019.0844294
Produced by FastQC (version 0.11.5)