##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049511_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6067761 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.761444295515265 31.0 31.0 34.0 30.0 34.0 2 31.974308974925016 33.0 31.0 34.0 30.0 34.0 3 31.985770039393444 33.0 31.0 34.0 30.0 34.0 4 35.62212354771389 37.0 35.0 37.0 33.0 37.0 5 35.74571823115644 37.0 35.0 37.0 35.0 37.0 6 35.799960150045465 37.0 35.0 37.0 35.0 37.0 7 35.7905532205372 37.0 35.0 37.0 35.0 37.0 8 35.77708383701995 37.0 35.0 37.0 33.0 37.0 9 37.526195412113296 39.0 37.0 39.0 35.0 39.0 10-11 37.48312927948217 39.0 37.0 39.0 34.5 39.0 12-13 37.46383509502105 39.0 37.0 39.0 34.5 39.0 14-15 38.73170078716021 40.0 38.0 41.0 34.5 41.0 16-17 38.734801519044666 40.0 38.0 41.0 34.5 41.0 18-19 38.809287066514315 40.0 38.0 41.0 35.0 41.0 20-21 38.8352379073599 40.0 38.0 41.0 35.0 41.0 22-23 38.797526632970545 40.0 38.0 41.0 35.0 41.0 24-25 38.753851379446225 40.0 38.0 41.0 34.0 41.0 26-27 38.65306980614431 40.0 38.0 41.0 34.0 41.0 28-29 38.56111323435448 40.0 38.0 41.0 34.0 41.0 30-31 38.43363853652113 40.0 38.0 41.0 34.0 41.0 32-33 38.26625455419223 40.0 38.0 41.0 34.0 41.0 34-35 38.14927713863483 40.0 38.0 41.0 34.0 41.0 36-37 37.99819546287337 40.0 37.5 41.0 33.0 41.0 38-39 37.88935185482751 40.0 37.0 41.0 33.0 41.0 40-41 37.75676324430049 40.0 37.0 41.0 33.0 41.0 42-43 37.607110843686826 40.0 37.0 41.0 33.0 41.0 44-45 37.41814245814889 40.0 37.0 41.0 32.5 41.0 46-47 37.167843212677624 40.0 37.0 41.0 31.5 41.0 48-49 36.931078119260135 40.0 37.0 41.0 31.0 41.0 50-51 36.71931335133338 40.0 36.0 41.0 31.0 41.0 52-53 36.60824973165555 40.0 36.0 41.0 31.0 41.0 54-55 36.47316753247203 39.5 36.0 41.0 30.0 41.0 56-57 36.30350148926433 39.0 35.0 41.0 30.0 41.0 58-59 36.13257657973015 39.0 35.0 41.0 30.0 41.0 60-61 35.941975631538554 39.0 35.0 41.0 29.5 41.0 62-63 35.73302384520419 39.0 35.0 41.0 29.0 41.0 64-65 35.44748919741565 38.0 35.0 40.0 29.0 41.0 66-67 35.19378218753178 37.5 35.0 40.0 29.0 41.0 68-69 34.88141268253644 37.0 34.0 40.0 28.0 41.0 70-71 34.58129077595508 36.5 34.0 39.5 28.0 41.0 72-73 34.25908362244327 36.0 34.0 39.0 28.0 41.0 74-75 33.92863619710796 35.5 34.0 39.0 28.0 40.5 76-77 33.63782629210346 35.0 34.0 38.0 27.5 40.0 78-79 33.31022085411736 35.0 34.0 37.0 27.0 39.0 80-81 33.00029277685789 35.0 34.0 37.0 26.5 39.0 82-83 32.70673020905075 35.0 33.0 36.0 26.0 39.0 84-85 32.430473612919165 35.0 33.0 36.0 26.0 37.0 86-87 32.15018282691095 35.0 33.0 35.5 26.0 37.0 88-89 31.938283577748038 35.0 33.0 35.0 25.5 37.0 90-91 31.784651702662646 35.0 33.0 35.0 25.0 36.0 92-93 31.6200091763667 35.0 33.0 35.0 25.0 36.0 94-95 31.431865065219277 35.0 33.0 35.0 25.0 36.0 96-97 31.252205549954915 35.0 33.0 35.0 24.0 35.5 98-99 31.064295297721845 35.0 33.0 35.0 23.5 35.0 100 30.944998987270594 35.0 33.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.364425949698969 1101 2 0.3812637452988348 1101 3 0.472604538241125 1101 4 0.35295174867309953 1101 5 0.299100113227567 1101 6 0.2933142382097813 1101 7 0.27401629693412843 1101 8 0.32496896831180067 1101 9 0.3511894310521839 1101 10-11 0.34777147110440865 1101 12-13 0.2916162661205206 1101 14-15 0.4494665853344202 1101 16-17 0.45303616635689536 1101 18-19 0.4206146978507661 1101 20-21 0.3604704911382015 1101 22-23 0.40776120873750443 1101 24-25 0.4428707311880373 1101 26-27 0.4215814366651358 1101 28-29 0.4340061474395611 1101 30-31 0.46552414460302316 1101 32-33 0.5060283283046232 1101 34-35 0.48149492094211865 1101 36-37 0.49677453376980907 1101 38-39 0.4785741769085021 1101 40-41 0.5111937621371467 1101 42-43 0.447219344310156 1101 44-45 0.45786737347917494 1101 46-47 0.5667367974424877 1101 48-49 0.6043079413110277 1101 50-51 0.6439519819037116 1101 52-53 0.5978843428497385 1101 54-55 0.5428201173738927 1101 56-57 0.5130166998803816 1101 58-59 0.5694306877203132 1101 60-61 0.5899123335762013 1101 62-63 0.6100345643005483 1101 64-65 0.5805368331509726 1101 66-67 0.5737878988091865 1101 68-69 0.5671181830788754 1101 70-71 0.5460144438526342 1101 72-73 0.5362647035256956 1101 74-75 0.5495665770200588 1101 76-77 0.5171909076034353 1101 78-79 0.49335519205887834 1101 80-81 0.5151550372046501 1101 82-83 0.5024172353147449 1101 84-85 0.5198516737463663 1101 86-87 0.5972127923327051 1101 88-89 0.5462949308724276 1101 90-91 0.4343680098875815 1101 92-93 0.49527766727673495 1101 94-95 0.4550732292558486 1101 96-97 0.4803488603513344 1101 98-99 0.5663383497715628 1101 100 0.5893434161225457 1102 1 0.30791825699901665 1102 2 0.3871808225943987 1102 3 0.4510281449386362 1102 4 0.4216611096139218 1102 5 0.3152174841888069 1102 6 0.2607556614947484 1102 7 0.2877366650318649 1102 8 0.28971966362911417 1102 9 0.343843883919952 1102 10-11 0.34094316540515734 1102 12-13 0.3496831397950686 1102 14-15 0.3948149398555145 1102 16-17 0.4747990755957403 1102 18-19 0.4426830973281142 1102 20-21 0.4222037782731647 1102 22-23 0.4142604727055996 1102 24-25 0.42428169156173823 1102 26-27 0.42711404946193454 1102 28-29 0.4013318932068799 1102 30-31 0.4835422533167133 1102 32-33 0.5229105886411958 1102 34-35 0.5012948780018505 1102 36-37 0.5487380688996524 1102 38-39 0.554769254919627 1102 40-41 0.5913923887335173 1102 42-43 0.6146239341642712 1102 44-45 0.6681425918784143 1102 46-47 0.6548393304398274 1102 48-49 0.6837610842828283 1102 50-51 0.7658901074561868 1102 52-53 0.7151061069022404 1102 54-55 0.7121172808403244 1102 56-57 0.6378074637221403 1102 58-59 0.647049460182842 1102 60-61 0.7188938906279887 1102 62-63 0.7095153476267342 1102 64-65 0.7400294302850625 1102 66-67 0.7421149233043991 1102 68-69 0.6930642576456876 1102 70-71 0.6799079197368556 1102 72-73 0.6864213092786358 1102 74-75 0.6752654847203559 1102 76-77 0.6361493130215052 1102 78-79 0.6138175184007508 1102 80-81 0.6152862350781376 1102 82-83 0.5890646341390564 1102 84-85 0.5669473226332045 1102 86-87 0.6605382565857525 1102 88-89 0.6396496518947004 1102 90-91 0.6637538036555206 1102 92-93 0.730568362876479 1102 94-95 0.6888012207078695 1102 96-97 0.7197299458016353 1102 98-99 0.7563419728386727 1102 100 0.8120878446062747 1103 1 0.3341663380213973 1103 2 0.3443777139705553 1103 3 0.4297454697999079 1103 4 0.3479424179022246 1103 5 0.2460851819162002 1103 6 0.2779683444346901 1103 7 0.27973129192830015 1103 8 0.3097897968698433 1103 9 0.37704875065051624 1103 10-11 0.33249312515619067 1103 12-13 0.29724436047525415 1103 14-15 0.42527687180453455 1103 16-17 0.4018309931575388 1103 18-19 0.40828955369863706 1103 20-21 0.44404521051896495 1103 22-23 0.36750055669040194 1103 24-25 0.3904940270980859 1103 26-27 0.39044023995758437 1103 28-29 0.4555188765892666 1103 30-31 0.4309926939275499 1103 32-33 0.4167576204142023 1103 34-35 0.4061151687495439 1103 36-37 0.4206078480089275 1103 38-39 0.4728629568903031 1103 40-41 0.5343228944371035 1103 42-43 0.4607804433005498 1103 44-45 0.4406627715826019 1103 46-47 0.46636268000762016 1103 48-49 0.4686161045822601 1103 50-51 0.5203715564216651 1103 52-53 0.4919214392961493 1103 54-55 0.4507999247850165 1103 56-57 0.5048495082034492 1103 58-59 0.5079223652489802 1103 60-61 0.5123209511051883 1103 62-63 0.4648954796400986 1103 64-65 0.5261237823456142 1103 66-67 0.5606746851326534 1103 68-69 0.5704487023811851 1103 70-71 0.5635501076783456 1103 72-73 0.5620638590116229 1103 74-75 0.49396230311000977 1103 76-77 0.49412748759156955 1103 78-79 0.47064826138309 1103 80-81 0.4657625589793355 1103 82-83 0.5069177339579056 1103 84-85 0.5129157961115496 1103 86-87 0.5675665900070115 1103 88-89 0.5666429517945204 1103 90-91 0.4444488026851836 1103 92-93 0.46884839557280245 1103 94-95 0.5330035491563159 1103 96-97 0.5759477440112839 1103 98-99 0.601932064831388 1103 100 0.6151308380134424 1104 1 0.2894523230946149 1104 2 0.2698150053652917 1104 3 0.37618735241199985 1104 4 0.2720160355928627 1104 5 0.19502804575267874 1104 6 0.20367155524428426 1104 7 0.16314723066339099 1104 8 0.2148097104003952 1104 9 0.2190116862304734 1104 10-11 0.2489183649856841 1104 12-13 0.25710513310198024 1104 14-15 0.3156061068146201 1104 16-17 0.28879972480712013 1104 18-19 0.2980017583044585 1104 20-21 0.3211329365428597 1104 22-23 0.3217249938935609 1104 24-25 0.2850591088972081 1104 26-27 0.3177124970170766 1104 28-29 0.3110957300384527 1104 30-31 0.3065274992495972 1104 32-33 0.2894500697023972 1104 34-35 0.2411106505535443 1104 36-37 0.33545430540857524 1104 38-39 0.3303414079857774 1104 40-41 0.3288003506166888 1104 42-43 0.32696273501476014 1104 44-45 0.3707807314877911 1104 46-47 0.39956183661103495 1104 48-49 0.3365095299225018 1104 50-51 0.4051399114027845 1104 52-53 0.3496107395386332 1104 54-55 0.3150085102701894 1104 56-57 0.34350244685638387 1104 58-59 0.27221462103060645 1104 60-61 0.29152173243362967 1104 62-63 0.29556079541180225 1104 64-65 0.27718426532098306 1104 66-67 0.32475365772226894 1104 68-69 0.28236396110496287 1104 70-71 0.27193174159189226 1104 72-73 0.3199585317999194 1104 74-75 0.3032643102843622 1104 76-77 0.24597477560907066 1104 78-79 0.24943464655245862 1104 80-81 0.2659715825926128 1104 82-83 0.2589539647175414 1104 84-85 0.25915139341098126 1104 86-87 0.28057443067442733 1104 88-89 0.2891329189653362 1104 90-91 0.2700284857116735 1104 92-93 0.28820939791253153 1104 94-95 0.2965199530982545 1104 96-97 0.23816254239629586 1104 98-99 0.3027617699164118 1104 100 0.2847116650410797 1105 1 0.22154283255874319 1105 2 0.21391173287622323 1105 3 0.3215970468950111 1105 4 0.25582808768607634 1105 5 0.14862584843251625 1105 6 0.18732850402778922 1105 7 0.1807206476118708 1105 8 0.22589787644552217 1105 9 0.2749253072522677 1105 10-11 0.23618419000244018 1105 12-13 0.23199953661174533 1105 14-15 0.29234786679855773 1105 16-17 0.2806705970127581 1105 18-19 0.30516481939893936 1105 20-21 0.30817207258650114 1105 22-23 0.3352878494537137 1105 24-25 0.33965242546317853 1105 26-27 0.32368575114697506 1105 28-29 0.36155615433801813 1105 30-31 0.30868023718396387 1105 32-33 0.29361035441023375 1105 34-35 0.27839577807744575 1105 36-37 0.32205724456392204 1105 38-39 0.28250039057389387 1105 40-41 0.32421718496387797 1105 42-43 0.24955284882206286 1105 44-45 0.28400764250550736 1105 46-47 0.26273518898467074 1105 48-49 0.26680073501498924 1105 50-51 0.30006117197326887 1105 52-53 0.26060370781040376 1105 54-55 0.20919101028543707 1105 56-57 0.2527875082494262 1105 58-59 0.2875476554534231 1105 60-61 0.2719188310691223 1105 62-63 0.23999321436249232 1105 64-65 0.2698713936302113 1105 66-67 0.2927853507146665 1105 68-69 0.2513103958153735 1105 70-71 0.23968825712473318 1105 72-73 0.2572707799604288 1105 74-75 0.2749251401915487 1105 76-77 0.2623720444093607 1105 78-79 0.23148595190951937 1105 80-81 0.19485225150481256 1105 82-83 0.20872246118308624 1105 84-85 0.23643481804018762 1105 86-87 0.2848644273824661 1105 88-89 0.20099513281547132 1105 90-91 0.17839210377665538 1105 92-93 0.24827319449718388 1105 94-95 0.2987893364517582 1105 96-97 0.3047775748719239 1105 98-99 0.34511600774716555 1105 100 0.34463605317504076 1106 1 0.17746318634654656 1106 2 0.23267796571387223 1106 3 0.3262281143604646 1106 4 0.27841561760363476 1106 5 0.20963111012250835 1106 6 0.18409482103482588 1106 7 0.18443780067902793 1106 8 0.21700587804560456 1106 9 0.22250948527060643 1106 10-11 0.2473689402573953 1106 12-13 0.18868003612102768 1106 14-15 0.2854923211304694 1106 16-17 0.33822477013448804 1106 18-19 0.2910576065148973 1106 20-21 0.291527565564742 1106 22-23 0.23030214289255468 1106 24-25 0.2998325045094532 1106 26-27 0.33724398556129387 1106 28-29 0.337904954266925 1106 30-31 0.29552277306823527 1106 32-33 0.3326749163561118 1106 34-35 0.3286033414976899 1106 36-37 0.375076198462331 1106 38-39 0.38231605051270634 1106 40-41 0.37901942962785284 1106 42-43 0.40957709454636415 1106 44-45 0.38674515323782543 1106 46-47 0.4301116049161635 1106 48-49 0.38993571021429574 1106 50-51 0.4334951094706341 1106 52-53 0.46929352334413466 1106 54-55 0.4476236648475833 1106 56-57 0.48472375862924366 1106 58-59 0.5263568888152932 1106 60-61 0.475713470113007 1106 62-63 0.504489260752969 1106 64-65 0.47492578413353215 1106 66-67 0.4953125063769619 1106 68-69 0.5034119970394642 1106 70-71 0.5222996392723331 1106 72-73 0.48223121699242455 1106 74-75 0.49125459867819643 1106 76-77 0.41793789826405714 1106 78-79 0.4657426427034608 1106 80-81 0.3414760684067488 1106 82-83 0.3687102726985714 1106 84-85 0.3811639601094683 1106 86-87 0.4524963926386576 1106 88-89 0.48812857372309537 1106 90-91 0.4161036861168661 1106 92-93 0.45772674878682196 1106 94-95 0.42971071716359077 1106 96-97 0.4476427658519633 1106 98-99 0.4513235979442065 1106 100 0.520464761671235 1107 1 0.2157416563198744 1107 2 0.2030736651089704 1107 3 0.2614620294566059 1107 4 0.20886410179662818 1107 5 0.15985882286831554 1107 6 0.16423151165032834 1107 7 0.13070954733422013 1107 8 0.18471506522842418 1107 9 0.19351078889389584 1107 10-11 0.14981371544859456 1107 12-13 0.13766754502452017 1107 14-15 0.15063747816716955 1107 16-17 0.1575286271481815 1107 18-19 0.18766927400817224 1107 20-21 0.2397146281916207 1107 22-23 0.22576983418478136 1107 24-25 0.27205942746955714 1107 26-27 0.2399623359805645 1107 28-29 0.24387019758462714 1107 30-31 0.2597673530955973 1107 32-33 0.243718577087364 1107 34-35 0.201427749977924 1107 36-37 0.2953223696360894 1107 38-39 0.277661875529013 1107 40-41 0.25583723776280465 1107 42-43 0.2402842175818023 1107 44-45 0.24227391240106755 1107 46-47 0.19313512847342906 1107 48-49 0.2117427660962008 1107 50-51 0.22328754371442727 1107 52-53 0.20787856656797032 1107 54-55 0.21273908106806516 1107 56-57 0.18093700521239953 1107 58-59 0.19682233687166928 1107 60-61 0.16434651256493993 1107 62-63 0.15759236431722456 1107 64-65 0.1456974257802841 1107 66-67 0.15206983881812164 1107 68-69 0.21420547794144795 1107 70-71 0.22420900337677807 1107 72-73 0.2099250955374714 1107 74-75 0.22699719123100692 1107 76-77 0.17901038538616376 1107 78-79 0.15425133488353282 1107 80-81 0.20270155856651684 1107 82-83 0.17904971710947137 1107 84-85 0.18908294378334034 1107 86-87 0.24176164330879857 1107 88-89 0.21295925918929726 1107 90-91 0.2087939227025437 1107 92-93 0.2172292601923651 1107 94-95 0.1594684189862896 1107 96-97 0.1595552603005892 1107 98-99 0.22608047192259662 1107 100 0.23259759790288825 1108 1 0.043978725641800054 1108 2 0.08966272503983319 1108 3 0.17439544116932737 1108 4 0.14291376908337128 1108 5 0.12079981828347996 1108 6 0.13705730834799823 1108 7 0.09310596839056018 1108 8 0.1548154914502149 1108 9 0.11697212281040237 1108 10-11 0.11765974785374311 1108 12-13 0.10244603892036963 1108 14-15 0.12765017083789587 1108 16-17 0.13442797545764051 1108 18-19 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-0.7069808175724361 2215 24-25 -0.6639847057398285 2215 26-27 -0.6792260846363547 2215 28-29 -0.6494798970348796 2215 30-31 -0.600062369391452 2215 32-33 -0.6566900434149829 2215 34-35 -0.6805371040653512 2215 36-37 -0.7471739593546332 2215 38-39 -0.7113199390903304 2215 40-41 -0.7255749704657646 2215 42-43 -0.6736176905300013 2215 44-45 -0.7538077088532233 2215 46-47 -0.7127164689504184 2215 48-49 -0.7414925137638235 2215 50-51 -0.7431086384969063 2215 52-53 -0.8983981758542541 2215 54-55 -0.7763942284236478 2215 56-57 -0.6630735306274147 2215 58-59 -0.6766605726225734 2215 60-61 -0.8405135083884474 2215 62-63 -0.7657264282726146 2215 64-65 -0.7168897991175953 2215 66-67 -0.6365680609976891 2215 68-69 -0.5930569770925587 2215 70-71 -0.720096163414425 2215 72-73 -0.5816878995457273 2215 74-75 -0.7078769598941506 2215 76-77 -0.679939844681364 2215 78-79 -0.6230748817375655 2215 80-81 -0.7092208500830495 2215 82-83 -0.7030361997007688 2215 84-85 -0.7005130058655276 2215 86-87 -0.6601726373679924 2215 88-89 -0.6171478593826976 2215 90-91 -0.5913034305630305 2215 92-93 -0.7410632370055339 2215 94-95 -0.8114144992681638 2215 96-97 -0.9094235197464791 2215 98-99 -0.8942082555357196 2215 100 -0.9477745325284346 2216 1 -0.7123252329350436 2216 2 -1.1691122303029609 2216 3 -0.7297211125502656 2216 4 -1.1346060365752706 2216 5 -0.6292975865950652 2216 6 -0.8634177932617888 2216 7 -0.607279392986861 2216 8 -0.5736268395390169 2216 9 -0.6440421866582042 2216 10-11 -0.8721705441054084 2216 12-13 -0.6946674233919268 2216 14-15 -0.9509630178891086 2216 16-17 -0.9159962414177869 2216 18-19 -0.993622131722077 2216 20-21 -0.8723665956434417 2216 22-23 -0.8336674815670477 2216 24-25 -0.9114500587889225 2216 26-27 -1.0764522455085341 2216 28-29 -1.3137775555374063 2216 30-31 -1.3611751086374966 2216 32-33 -1.12372663191681 2216 34-35 -1.1361101381283234 2216 36-37 -1.1128470087891174 2216 38-39 -1.2541007169461622 2216 40-41 -1.0814848848019238 2216 42-43 -1.639644787484002 2216 44-45 -1.3599694573552128 2216 46-47 -1.2752512164626069 2216 48-49 -1.2063714882686654 2216 50-51 -1.3836994072591224 2216 52-53 -1.274383784099129 2216 54-55 -1.483540399984058 2216 56-57 -1.6187064704788412 2216 58-59 -1.3903509128500957 2216 60-61 -1.5706258640650574 2216 62-63 -1.560382530258984 2216 64-65 -1.568973931553927 2216 66-67 -1.4278850258285445 2216 68-69 -1.6668660083570614 2216 70-71 -1.443960575209978 2216 72-73 -1.2946130402955944 2216 74-75 -1.407049987061498 2216 76-77 -1.4843685632577603 2216 78-79 -1.512310946458804 2216 80-81 -1.3487643449896218 2216 82-83 -1.4485676157615508 2216 84-85 -1.442987574637229 2216 86-87 -1.6610116057242337 2216 88-89 -1.4817247190839176 2216 90-91 -1.3403744598651492 2216 92-93 -1.4188447259732442 2216 94-95 -1.5532432491622181 2216 96-97 -1.6353254466441776 2216 98-99 -1.6376643741865315 2216 100 -1.667914944800934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 365.0 3 34.0 4 72.0 5 138.0 6 420.0 7 1128.0 8 2425.0 9 4177.0 10 5999.0 11 8768.0 12 12689.0 13 18008.0 14 20370.0 15 24740.0 16 30255.0 17 41076.0 18 42159.0 19 24104.0 20 17643.0 21 16844.0 22 17716.0 23 19169.0 24 22274.0 25 27498.0 26 35030.0 27 44844.0 28 58144.0 29 75474.0 30 97901.0 31 125592.0 32 162040.0 33 209760.0 34 284093.0 35 409363.0 36 658716.0 37 1212323.0 38 1668923.0 39 665283.0 40 2204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12596750170513 20.507375242246464 14.35202286516387 23.01463439088454 2 16.3165945064636 20.970115935909675 38.882541120273764 23.830748437352963 3 18.006667372034283 25.26855883177938 31.009987543998953 25.714786252187384 4 12.865631682020826 17.836894300258407 37.84343493593757 31.454039081783193 5 12.408030718971807 36.86794115218559 35.210627638273856 15.513400490568754 6 29.95162195177424 38.10642313602614 17.602364548386948 14.339590363812679 7 25.94462768062223 32.7368365365742 22.684100444958197 18.634435337845375 8 24.667945886464544 35.14222132348324 21.067375593732184 19.122457196320024 9 24.19707974462392 16.677843229384433 22.45132756634783 36.673749459643815 10-11 23.37140503721224 26.828775886195917 28.808319905810396 20.991499170781445 12-13 24.571674649569967 24.37108988672754 29.112197765270505 21.94503769843199 14-15 21.881247930146213 26.57370484113617 27.120875506024916 24.4241717226927 16-17 20.44310084065605 29.679374319456553 27.663828552245217 22.21369628764218 18-19 20.178835323276576 27.632045823822 31.073043252692383 21.11607560020904 20-21 21.532201087023697 27.171332226170414 30.712893932374723 20.583572754431163 22-23 21.074635273208685 27.298191210893112 30.752504919030265 20.874668596867938 24-25 20.91171685898637 27.138807873283078 30.964650717126137 20.984824550604415 26-27 20.760441948850655 26.861728733218072 31.075177483094667 21.302651834836606 28-29 20.825605322826206 26.93325240278363 31.13146149300054 21.109680781389624 30-31 21.346437486542573 27.501392403488246 30.49181940804867 20.660350701920507 32-33 20.67232218542574 27.255475887685414 31.06023218945441 21.01196973743443 34-35 20.680082818027934 26.696676088593467 31.149628339019948 21.47361275435865 36-37 21.484168542564547 27.30916725296201 30.277568612211326 20.92909559226212 38-39 21.332028403887364 27.296856286857707 30.43939931055294 20.931715998701993 40-41 20.981709727855137 27.092069051500218 30.631875579806128 21.294345640838525 42-43 20.96072999579252 27.06268424217763 30.434339783653314 21.54224597837654 44-45 21.296133779824224 27.303267218336387 30.16194111798405 21.238657883855346 46-47 21.21612898068991 27.756820019773354 29.609628658742494 21.41742234079424 48-49 21.111320963366882 27.338906393973 29.7631449228142 21.786627719845917 50-51 21.24621421311749 27.19428962347067 29.576832376884983 21.98266378652686 52-53 21.124859729972883 27.41117357786505 29.417152389489303 22.046814302672765 54-55 21.26148343680643 27.88405805699994 29.172729446660806 21.681729059532834 56-57 20.844360877101124 27.55125819886446 29.255824347728925 22.348556576305494 58-59 20.61271866446574 27.009189906495724 29.757369460544147 22.620721968494387 60-61 21.100880538966514 27.44011341250916 29.176379887078614 22.282626161445712 62-63 20.88985541783864 27.424184967074343 29.246587003014785 22.439372612072226 64-65 20.75474792102062 27.099468815597717 29.538465671274793 22.607317592106874 66-67 20.721399540950937 27.16751698031613 29.136950186403187 22.97413329232974 68-69 21.256217903111214 27.326751993033344 28.918187450033052 22.49884265382239 70-71 21.165204100820713 26.982226228093033 29.013428511769 22.83914115931725 72-73 20.794260024414278 27.09505202990032 29.263520761612067 22.84716718407333 74-75 20.827896813997782 27.027036826269196 29.037259377882553 23.10780698185047 76-77 20.856342232332487 27.162869467007685 28.688053138546493 23.29273516211334 78-79 20.99309901102607 27.249483684530034 28.705557996052413 23.051859308391485 80-81 20.699625931720075 26.95701902313752 28.836812043358794 23.506543001783612 82-83 20.506456890680514 26.915422004147345 28.9407633893495 23.637357715822645 84-85 20.609257681705 26.840394669467038 28.704747929260893 23.84559971956707 86-87 20.809278943900132 26.988174135774496 28.574360931740948 23.62818598858442 88-89 20.511757516966938 26.764711141589153 28.657741951246724 24.065789390197185 90-91 20.308896681261295 26.560671430975237 28.639907629788077 24.490524257975395 92-93 20.445363112324014 26.69652190780627 28.49167636690073 24.36643861296898 94-95 20.319734226671702 26.439258061544297 28.65597776564437 24.585029946139635 96-97 20.272512403876192 26.359412175216868 28.707420142901157 24.66065527800578 98-99 20.248388223033558 26.357191645450023 28.630782086864187 24.763638044652232 100 20.386852646437646 26.349901156701446 28.433578869041487 24.829667327819426 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1272.0 1 999.0 2 2236.5 3 3422.5 4 1978.5 5 976.5 6 1931.5 7 4361.5 8 7029.5 9 8015.0 10 6208.5 11 4002.5 12 3669.0 13 4019.0 14 4505.5 15 5127.5 16 5704.5 17 6481.0 18 7465.0 19 8461.0 20 10045.5 21 12116.0 22 14928.0 23 19323.0 24 25184.5 25 32429.0 26 42424.0 27 54935.5 28 70230.0 29 87725.0 30 104644.0 31 120077.0 32 134026.5 33 147228.0 34 160066.0 35 171642.5 36 181207.5 37 190393.0 38 198554.0 39 204065.5 40 207157.5 41 208768.0 42 207320.5 43 205625.5 44 204611.0 45 202813.5 46 200737.5 47 197045.5 48 194299.5 49 192107.5 50 187926.0 51 181272.5 52 172297.0 53 165369.5 54 161420.0 55 155642.0 56 147466.0 57 136209.5 58 123199.5 59 109740.5 60 93641.5 61 76801.5 62 62399.0 63 49872.5 64 38972.5 65 29986.0 66 23057.0 67 17433.5 68 12688.5 69 9344.0 70 6798.0 71 5034.5 72 3785.5 73 2744.0 74 1937.5 75 1298.0 76 857.5 77 594.5 78 416.0 79 261.5 80 151.5 81 78.5 82 42.5 83 27.0 84 15.5 85 4.5 86 4.5 87 3.5 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06079672551374387 2 1.977665237638727E-4 3 4.9441630940968176E-5 4 0.11846214773455976 5 0.001812859801168833 6 0.002191912305049589 7 0.0 8 0.0 9 6.592217458795757E-5 10-11 0.0 12-13 3.2961087293978786E-5 14-15 2.4720815470484088E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.1536380552892575E-4 30-31 2.5544842652833557E-4 32-33 8.240271823494697E-6 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.8016924199881967E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.2360407735242045E-4 80-81 3.048900574693038E-4 82-83 3.048900574693038E-4 84-85 0.0 86-87 2.4720815470484088E-5 88-89 7.416244641145226E-5 90-91 6.592217458795757E-5 92-93 6.262606585855969E-4 94-95 5.603384839976393E-4 96-97 8.240271823494697E-6 98-99 2.4720815470484088E-5 100 0.0012525213171711938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6067761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.1735260158344 #Duplication Level Percentage of deduplicated Percentage of total 1 78.46280717491089 51.13697804689452 2 13.489113677064408 17.58266202325409 3 3.844850286674987 7.5174735055680175 4 1.5564356946867122 4.057536089585509 5 0.7661324051044147 2.4965775117823172 6 0.4500554737872856 1.7599021277666598 7 0.3139448812101095 1.4322626418159576 8 0.20822983893675215 1.0856858260173936 9 0.14200598238808324 0.8329527528816476 >10 0.7234701444344376 8.012253363926556 >50 0.03207472455974845 1.4040590957528223 >100 0.009969600814761238 1.1185698221618667 >500 4.803465666930259E-4 0.21676688426550847 >1k 3.286467758486428E-4 0.4473454982056586 >5k 2.5280521212576014E-5 0.09091571060904169 >10k+ 7.584156363772805E-5 0.8080590995124757 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19330 0.31856890869630494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19163 0.3158166579072577 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10551 0.17388621601938506 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.6480543646989393E-5 0.0 0.0 0.0 6 0.0 1.6480543646989393E-5 0.0 0.0 0.0 7 0.0 1.6480543646989393E-5 0.0 0.0 0.0 8 1.6480543646989393E-5 1.6480543646989393E-5 0.0 0.0 0.0 9 1.6480543646989393E-5 1.6480543646989393E-5 0.0 1.6480543646989393E-5 0.0 10-11 1.6480543646989393E-5 1.6480543646989393E-5 0.0 3.2961087293978786E-5 0.0 12-13 1.6480543646989393E-5 1.6480543646989393E-5 0.0 8.240271823494696E-5 0.0 14-15 1.6480543646989393E-5 4.1201359117473484E-5 0.0 9.888326188193635E-5 0.0 16-17 1.6480543646989393E-5 7.416244641145226E-5 0.0 1.0712353370543104E-4 0.0 18-19 1.6480543646989393E-5 8.240271823494696E-5 0.0 2.472081547048409E-4 0.0 20-21 1.6480543646989393E-5 8.240271823494696E-5 0.0 3.708122320572613E-4 0.0 22-23 1.6480543646989393E-5 8.240271823494696E-5 0.0 8.569882696434483E-4 0.0 24-25 1.6480543646989393E-5 8.240271823494696E-5 0.0 0.00203534714040319 1.6480543646989393E-5 26-27 1.6480543646989393E-5 8.240271823494696E-5 0.0 0.003881168028866002 1.6480543646989393E-5 28-29 1.6480543646989393E-5 9.888326188193635E-5 0.0 0.010539307662249716 1.6480543646989393E-5 30-31 1.6480543646989393E-5 1.0712353370543104E-4 0.0 0.02976386182646284 1.6480543646989393E-5 32-33 1.6480543646989393E-5 1.1536380552892575E-4 0.0 0.07611539083362051 1.6480543646989393E-5 34-35 1.6480543646989393E-5 1.1536380552892575E-4 0.0 0.15856755069950845 1.6480543646989393E-5 36-37 1.6480543646989393E-5 1.4832489282290453E-4 0.0 0.29316415066447077 1.6480543646989393E-5 38-39 1.6480543646989393E-5 1.8128598011688332E-4 0.0 0.4791223649052756 1.6480543646989393E-5 40-41 1.6480543646989393E-5 1.977665237638727E-4 0.0 0.692940938181316 1.6480543646989393E-5 42-43 1.6480543646989393E-5 2.472081547048409E-4 0.0 0.925176518982867 1.6480543646989393E-5 44-45 1.6480543646989393E-5 2.472081547048409E-4 0.0 1.1977399900886012 1.6480543646989393E-5 46-47 3.2961087293978786E-5 2.472081547048409E-4 0.0 1.522719830263585 1.6480543646989393E-5 48-49 3.2961087293978786E-5 2.472081547048409E-4 0.0 1.8747360022914548 1.6480543646989393E-5 50-51 3.2961087293978786E-5 3.1313032929279844E-4 0.0 2.238815932268921 1.6480543646989393E-5 52-53 4.9441630940968176E-5 3.1313032929279844E-4 0.0 2.6003743390683978 1.6480543646989393E-5 54-55 4.9441630940968176E-5 3.213706011162931E-4 0.0 3.0063066096373934 1.6480543646989393E-5 56-57 4.9441630940968176E-5 3.296108729397878E-4 0.0 3.476224590915826 1.6480543646989393E-5 58-59 4.9441630940968176E-5 3.296108729397878E-4 0.0 3.976862305552246 1.6480543646989393E-5 60-61 4.9441630940968176E-5 3.296108729397878E-4 0.0 4.480005062823008 1.6480543646989393E-5 62-63 8.240271823494696E-5 3.296108729397878E-4 0.0 4.97442137223269 1.6480543646989393E-5 64-65 8.240271823494696E-5 3.296108729397878E-4 0.0 5.51551882152247 1.6480543646989393E-5 66-67 8.240271823494696E-5 3.296108729397878E-4 0.0 6.126378412069955 1.6480543646989393E-5 68-69 8.240271823494696E-5 3.4609141658677726E-4 0.0 6.765378530894674 1.6480543646989393E-5 70-71 8.240271823494696E-5 3.79052503880756E-4 0.0 7.431958839512631 1.6480543646989393E-5 72-73 8.240271823494696E-5 4.449746784687136E-4 0.0 8.138932960609358 1.6480543646989393E-5 74-75 8.240271823494696E-5 4.8617603758618704E-4 0.0 8.82726758684134 1.6480543646989393E-5 76-77 8.240271823494696E-5 5.273773967036606E-4 0.0 9.540809204581393 1.6480543646989393E-5 78-79 8.240271823494696E-5 5.273773967036606E-4 0.0 10.309395838102391 1.6480543646989393E-5 80-81 9.888326188193635E-5 5.768190276446287E-4 0.0 11.109575673794666 1.6480543646989393E-5 82-83 9.888326188193635E-5 5.850592994681234E-4 0.0 11.919503751054137 1.6480543646989393E-5 84-85 9.888326188193635E-5 5.932995712916181E-4 0.0 12.704068271640889 1.6480543646989393E-5 86-87 9.888326188193635E-5 5.932995712916181E-4 0.0 13.511153455121255 1.6480543646989393E-5 88 9.888326188193635E-5 5.932995712916181E-4 0.0 14.146783302770165 1.6480543646989393E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11155 0.0 58.079014 1 GTATCAA 20050 0.0 39.216827 1 GTGGTAT 4540 0.0 33.808983 1 ATCAACG 24850 0.0 31.280296 3 TCAACGC 25060 0.0 31.093277 4 TGGTATC 4730 0.0 30.738634 2 TATCAAC 25660 0.0 30.421244 2 CAACGCA 25835 0.0 30.18007 5 AACGCAG 26915 0.0 29.12597 6 ACGCAGA 30475 0.0 25.630608 7 CGCAGAG 30740 0.0 25.317923 8 ATAACGC 585 0.0 24.934063 3 TATAACG 630 0.0 24.646805 2 GCAGAGT 33220 0.0 23.102455 9 GTACATG 13810 0.0 21.786003 1 GAGTACT 25710 0.0 21.305012 12-13 CAGAGTA 32990 0.0 20.756248 10-11 TACATGG 13945 0.0 20.41381 2 AGAGTAC 31255 0.0 20.292006 10-11 TAACGCA 715 0.0 19.084429 4 >>END_MODULE