FastQCFastQC Report
Wed 25 May 2016
SRR2049510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4106572
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.044.1767161
GTATCAA62000.036.7926451
ATCAACG62300.034.5503163
TCAACGC62200.034.275814
CAACGCA65400.032.755435
TATCAAC71950.030.415422
AACGCAG73650.029.4329916
ACGCAGA84350.025.4559767
ATAACGC2451.1459633E-1025.1018623
CGCAGAG84550.024.435698
GTCTCGC11900.023.2615971
GTACATG88450.022.7765221
TCTGTCG13950.022.6853718
TACCTGG26150.021.558462
GTGGTAT23600.021.2866231
TACATGG88950.021.0302122
TATAACG3201.4370016E-1020.8204522
TCGCTCT13250.020.499574
GCAGAGT95700.020.2659729
ACATGGG90450.019.8312573
Produced by FastQC (version 0.11.5)