FastQCFastQC Report
Wed 25 May 2016
SRR2049510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4106572
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130410.3175641386538456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128370.31259649167237297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72060.17547482425731242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76250.059.5392231
GTATCAA138050.039.9813271
GTGGTAT30050.034.3214531
ATCAACG169650.032.5408863
ATAACGC3950.032.1700633
TCAACGC173200.032.0641254
TATCAAC174400.031.7085692
CAACGCA178350.031.1744565
TGGTATC32450.030.0220872
TATAACG4100.029.8452152
AACGCAG188950.029.5741086
ACGCAGA214150.025.8738987
CGCAGAG213800.025.696448
GCAGAGT232600.023.15489
GCGGTAT1903.5749872E-722.3077071
GAGTACT176950.021.76529312-13
GTACATG92000.021.6530231
CAGAGTA226700.021.2697310-11
GTCTCGC14400.020.2766021
AGAGTAC216300.019.94583710-11
Produced by FastQC (version 0.11.5)