##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049510_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4106572
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.694214541958598	31.0	31.0	34.0	30.0	34.0
2	31.746086029905236	31.0	31.0	34.0	30.0	34.0
3	31.798342510492937	33.0	31.0	34.0	30.0	34.0
4	35.53027683430365	37.0	35.0	37.0	33.0	37.0
5	35.68868949576435	37.0	35.0	37.0	33.0	37.0
6	35.779672437254234	37.0	35.0	37.0	35.0	37.0
7	35.755662630534665	37.0	35.0	37.0	33.0	37.0
8	35.74783590790567	37.0	35.0	37.0	33.0	37.0
9	37.47633281481489	39.0	37.0	39.0	34.0	39.0
10-11	37.445354665643265	39.0	37.0	39.0	34.0	39.0
12-13	37.4452784463538	39.0	37.0	39.0	34.0	39.0
14-15	38.70457025957417	40.0	38.0	41.0	34.0	41.0
16-17	38.709117117634854	40.0	38.0	41.0	34.0	41.0
18-19	38.78054384532891	40.0	38.0	41.0	35.0	41.0
20-21	38.801974980592085	40.0	38.0	41.0	35.0	41.0
22-23	38.768371040371385	40.0	38.0	41.0	34.5	41.0
24-25	38.72704399192319	40.0	38.0	41.0	34.0	41.0
26-27	38.62660413600443	40.0	38.0	41.0	34.0	41.0
28-29	38.52991375775221	40.0	38.0	41.0	34.0	41.0
30-31	38.39236131741998	40.0	38.0	41.0	34.0	41.0
32-33	38.21835158375404	40.0	38.0	41.0	34.0	41.0
34-35	38.09723316673859	40.0	38.0	41.0	33.5	41.0
36-37	37.93300969762615	40.0	37.0	41.0	33.0	41.0
38-39	37.8186880687834	40.0	37.0	41.0	33.0	41.0
40-41	37.6801182348684	40.0	37.0	41.0	33.0	41.0
42-43	37.52787288278399	40.0	37.0	41.0	33.0	41.0
44-45	37.33872314913753	40.0	37.0	41.0	32.5	41.0
46-47	37.08337988473111	40.0	37.0	41.0	31.5	41.0
48-49	36.83331888494832	40.0	36.5	41.0	31.0	41.0
50-51	36.61313061112773	40.0	36.0	41.0	30.5	41.0
52-53	36.49727047279337	39.5	36.0	41.0	30.0	41.0
54-55	36.35708286619594	39.0	35.5	41.0	30.0	41.0
56-57	36.18556024343418	39.0	35.0	41.0	30.0	41.0
58-59	36.00874719839321	39.0	35.0	41.0	30.0	41.0
60-61	35.805673564228265	39.0	35.0	41.0	29.0	41.0
62-63	35.579343915070766	38.0	35.0	40.5	29.0	41.0
64-65	35.28872414753717	38.0	35.0	40.0	29.0	41.0
66-67	35.02555002079593	37.0	34.5	40.0	28.0	41.0
68-69	34.70049934592648	37.0	34.0	40.0	28.0	41.0
70-71	34.39666441986162	36.0	34.0	39.0	28.0	41.0
72-73	34.07860802148361	36.0	34.0	39.0	28.0	41.0
74-75	33.73922690263315	35.0	34.0	39.0	27.0	40.5
76-77	33.455968749604295	35.0	34.0	37.5	27.0	40.0
78-79	33.134038317117046	35.0	34.0	37.0	26.5	39.0
80-81	32.83496673137596	35.0	33.0	36.5	26.0	39.0
82-83	32.55684802312001	35.0	33.0	36.0	26.0	38.5
84-85	32.29039512761496	35.0	33.0	36.0	26.0	37.0
86-87	32.03060594091617	35.0	33.0	35.5	25.5	37.0
88-89	31.817481344537487	35.0	33.0	35.0	25.0	36.5
90-91	31.66568916848408	35.0	33.0	35.0	25.0	36.0
92-93	31.505992345927453	35.0	33.0	35.0	25.0	36.0
94-95	31.314011297013664	35.0	33.0	35.0	24.5	36.0
96-97	31.138258868954445	35.0	33.0	35.0	24.0	35.5
98-99	30.950613674860683	35.0	33.0	35.0	23.0	35.0
100	30.83125950305997	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3615172861778859
1101	2	0.4254355991008083
1101	3	0.4636386781026083
1101	4	0.39912265572586136
1101	5	0.26777140130101174
1101	6	0.24447075823437814
1101	7	0.27008354041120697
1101	8	0.2966705368651219
1101	9	0.28250328291358784
1101	10-11	0.3441138018495238
1101	12-13	0.3086780514808609
1101	14-15	0.453326479023076
1101	16-17	0.48088196183287835
1101	18-19	0.4872300453436722
1101	20-21	0.4706199166192704
1101	22-23	0.4708393682903278
1101	24-25	0.5110165412190781
1101	26-27	0.4785705596705938
1101	28-29	0.48581220245095125
1101	30-31	0.47911431753582434
1101	32-33	0.515197307210002
1101	34-35	0.5766581666032238
1101	36-37	0.5843898447962914
1101	38-39	0.5855314412317156
1101	40-41	0.6439374806100986
1101	42-43	0.5222496200688909
1101	44-45	0.5464715625964587
1101	46-47	0.6387132565227276
1101	48-49	0.6803932636496342
1101	50-51	0.7291119732674787
1101	52-53	0.7029327854148235
1101	54-55	0.6482987597792942
1101	56-57	0.6965691130119964
1101	58-59	0.6524524509558489
1101	60-61	0.7261418699793438
1101	62-63	0.6100518036030991
1101	64-65	0.595507807225168
1101	66-67	0.602649181462418
1101	68-69	0.6061433639948746
1101	70-71	0.5694029036676511
1101	72-73	0.5626655256681019
1101	74-75	0.5810098061039071
1101	76-77	0.568915753566813
1101	78-79	0.6014479456034962
1101	80-81	0.5732884815425479
1101	82-83	0.5521638933266431
1101	84-85	0.5899731078351067
1101	86-87	0.6927995260143298
1101	88-89	0.6572950924653895
1101	90-91	0.6382938097329003
1101	92-93	0.661978575123289
1101	94-95	0.6897313817684001
1101	96-97	0.7307818252984966
1101	98-99	0.8245313091542528
1101	100	0.8071651788960921
1102	1	0.3029907058171446
1102	2	0.41508357209819735
1102	3	0.4863217547621339
1102	4	0.3956693033522214
1102	5	0.3225662518538215
1102	6	0.29443174524298854
1102	7	0.3248021048401881
1102	8	0.3560563398836436
1102	9	0.42054489766331216
1102	10-11	0.3356506594233224
1102	12-13	0.3762336968547757
1102	14-15	0.5039417868375793
1102	16-17	0.45965601705600534
1102	18-19	0.4953592017663553
1102	20-21	0.4600637907766085
1102	22-23	0.4817485516674509
1102	24-25	0.5090613412490583
1102	26-27	0.44877614408253663
1102	28-29	0.47311765610579215
1102	30-31	0.5722890515716159
1102	32-33	0.533409147084086
1102	34-35	0.6446307758697145
1102	36-37	0.637671887979586
1102	38-39	0.6818695065353211
1102	40-41	0.6533424220772304
1102	42-43	0.6833465074612093
1102	44-45	0.7448880232682882
1102	46-47	0.7845706523285969
1102	48-49	0.8506449543241246
1102	50-51	0.8358990292521256
1102	52-53	0.8349426437233873
1102	54-55	0.8024599670808144
1102	56-57	0.7868417622952393
1102	58-59	0.7672584499895692
1102	60-61	0.724872910535467
1102	62-63	0.7040597962475061
1102	64-65	0.78508089936944
1102	66-67	0.83796089057941
1102	68-69	0.7693366476039216
1102	70-71	0.7618913971773082
1102	72-73	0.7507037945351982
1102	74-75	0.7282612304461438
1102	76-77	0.6887237132877431
1102	78-79	0.6312952261370128
1102	80-81	0.6441746184801573
1102	82-83	0.6675641211143315
1102	84-85	0.6988525449773562
1102	86-87	0.7636979757999889
1102	88-89	0.7225657676778852
1102	90-91	0.7345579979481727
1102	92-93	0.7820109766506214
1102	94-95	0.6990841274665982
1102	96-97	0.7338461853582956
1102	98-99	0.7966953055835262
1102	100	0.8769669821929895
1103	1	0.3172508599315904
1103	2	0.35752485015555635
1103	3	0.4259703197825466
1103	4	0.3434528568348867
1103	5	0.2746072965942119
1103	6	0.3012582851000545
1103	7	0.30496974836208324
1103	8	0.3350718644159585
1103	9	0.39968991621920225
1103	10-11	0.3371095762539511
1103	12-13	0.3083479041657995
1103	14-15	0.429376519546544
1103	16-17	0.4157693564154812
1103	18-19	0.4224272826540627
1103	20-21	0.4482208584356826
1103	22-23	0.4324141235178729
1103	24-25	0.45425082200753053
1103	26-27	0.49294488897646005
1103	28-29	0.49482278496450505
1103	30-31	0.4812160005355963
1103	32-33	0.4812933534453947
1103	34-35	0.5130240252044516
1103	36-37	0.6075986736527526
1103	38-39	0.5426695254780896
1103	40-41	0.563648725646523
1103	42-43	0.6750237474891492
1103	44-45	0.6106688134871945
1103	46-47	0.5461219125792383
1103	48-49	0.5411832861336663
1103	50-51	0.5859957227811208
1103	52-53	0.5580066446694829
1103	54-55	0.47640562203099535
1103	56-57	0.4848346437468791
1103	58-59	0.4771196502004216
1103	60-61	0.4848190814950044
1103	62-63	0.48828573433518585
1103	64-65	0.4638047388724118
1103	66-67	0.5148424421062074
1103	68-69	0.45317282265479264
1103	70-71	0.4837959685699502
1103	72-73	0.5277222671009127
1103	74-75	0.4272622451265349
1103	76-77	0.44732621554496177
1103	78-79	0.3939703594286499
1103	80-81	0.36656220465100375
1103	82-83	0.39384489955869384
1103	84-85	0.4240712472888646
1103	86-87	0.464555990157681
1103	88-89	0.5022176865003516
1103	90-91	0.46250447219907187
1103	92-93	0.4732251409781263
1103	94-95	0.5155968021729009
1103	96-97	0.48676093952538224
1103	98-99	0.5557802403616492
1103	100	0.5439711855731346
1104	1	0.23909025789788174
1104	2	0.2960271573851685
1104	3	0.3726305301290189
1104	4	0.27149024263773214
1104	5	0.1881621111429297
1104	6	0.23678427644984623
1104	7	0.2134999090996459
1104	8	0.23104906633697198
1104	9	0.28964848634166174
1104	10-11	0.2714069228112592
1104	12-13	0.28826993413111524
1104	14-15	0.35459514436476525
1104	16-17	0.3824567627553961
1104	18-19	0.36453069370262625
1104	20-21	0.34425838016629484
1104	22-23	0.335620765861222
1104	24-25	0.34933143017936175
1104	26-27	0.32089331517880737
1104	28-29	0.3717625180295272
1104	30-31	0.461979325912381
1104	32-33	0.45346206923304067
1104	34-35	0.4003933678110059
1104	36-37	0.4681514298205869
1104	38-39	0.44861703384486873
1104	40-41	0.39727566593268904
1104	42-43	0.42649949468727044
1104	44-45	0.477225820654148
1104	46-47	0.5262904669837383
1104	48-49	0.5262829383619376
1104	50-51	0.5649213510287865
1104	52-53	0.5614883890960058
1104	54-55	0.4939252391442608
1104	56-57	0.5117867974971801
1104	58-59	0.5648344601293758
1104	60-61	0.5725914519098936
1104	62-63	0.5355685102466055
1104	64-65	0.586840693928643
1104	66-67	0.5572178230327012
1104	68-69	0.40254507527887995
1104	70-71	0.49334698211303163
1104	72-73	0.5138141447731854
1104	74-75	0.5291387368548115
1104	76-77	0.4644611928833342
1104	78-79	0.44809354841430604
1104	80-81	0.4851950497655082
1104	82-83	0.4323697313213657
1104	84-85	0.48147483707945327
1104	86-87	0.51080030330969
1104	88-89	0.4777690472210452
1104	90-91	0.469126774410185
1104	92-93	0.5007641481819256
1104	94-95	0.4882928968026867
1104	96-97	0.5111029719621527
1104	98-99	0.5802849983181275
1104	100	0.636865755619425
1105	1	0.26165540516061014
1105	2	0.27880417892689024
1105	3	0.3784457400491341
1105	4	0.2660488433240431
1105	5	0.20772262859155433
1105	6	0.23602795248877584
1105	7	0.22707574079588255
1105	8	0.25929713215717953
1105	9	0.26858630129783023
1105	10-11	0.21760033594807737
1105	12-13	0.22372705688939476
1105	14-15	0.2964835620905433
1105	16-17	0.27886574512315576
1105	18-19	0.2776846147270291
1105	20-21	0.30611470225255744
1105	22-23	0.31165355311748044
1105	24-25	0.3157039271742761
1105	26-27	0.27119244757474803
1105	28-29	0.29021649662632854
1105	30-31	0.2699371236730741
1105	32-33	0.3057258698138128
1105	34-35	0.26769557723979176
1105	36-37	0.3383725721668114
1105	38-39	0.3118931313848847
1105	40-41	0.28975034680915
1105	42-43	0.27952437816993836
1105	44-45	0.31828872876928926
1105	46-47	0.2539409824525549
1105	48-49	0.3372374903092137
1105	50-51	0.3989766288715799
1105	52-53	0.3044349914783737
1105	54-55	0.2465897185681598
1105	56-57	0.23291196598407282
1105	58-59	0.27240193363910237
1105	60-61	0.3096689496669569
1105	62-63	0.3077364477782325
1105	64-65	0.27446077327373786
1105	66-67	0.27761182880988855
1105	68-69	0.3062467688528656
1105	70-71	0.2955683382160146
1105	72-73	0.2285016770338899
1105	74-75	0.3008845071873196
1105	76-77	0.22073473555539636
1105	78-79	0.24737761911015355
1105	80-81	0.20866269599242315
1105	82-83	0.22292154117892693
1105	84-85	0.2912620699895996
1105	86-87	0.33981371709765185
1105	88-89	0.31825050147280365
1105	90-91	0.31886861205948946
1105	92-93	0.33073349772605454
1105	94-95	0.336610276775847
1105	96-97	0.378483673946441
1105	98-99	0.43449042278184535
1105	100	0.4077761220597864
1106	1	0.22188215938898992
1106	2	0.20289227169842405
1106	3	0.3259588502001556
1106	4	0.26293063905031744
1106	5	0.2089888851177335
1106	6	0.2340627573211762
1106	7	0.22125595118045283
1106	8	0.21068095454236868
1106	9	0.20676415876754106
1106	10-11	0.19914481272103046
1106	12-13	0.18453792815301284
1106	14-15	0.26199749063038524
1106	16-17	0.2754673122230997
1106	18-19	0.21467666363037097
1106	20-21	0.2133365889094634
1106	22-23	0.24263756793870073
1106	24-25	0.2617161095892797
1106	26-27	0.2833735468340919
1106	28-29	0.32086500774229165
1106	30-31	0.2756443707279175
1106	32-33	0.25247578163589424
1106	34-35	0.2850700340163428
1106	36-37	0.2995645844362542
1106	38-39	0.30875526153219823
1106	40-41	0.3350304950815044
1106	42-43	0.34246353498322435
1106	44-45	0.3339953470894912
1106	46-47	0.35381538589474815
1106	48-49	0.3788681582368625
1106	50-51	0.4059892756479826
1106	52-53	0.4473210939899346
1106	54-55	0.38760850739593167
1106	56-57	0.3811661606732031
1106	58-59	0.40104362528677484
1106	60-61	0.4085848382236037
1106	62-63	0.32394325786719946
1106	64-65	0.35539731815979536
1106	66-67	0.36033615573723665
1106	68-69	0.35633027590272803
1106	70-71	0.29105559299424044
1106	72-73	0.3513871338941925
1106	74-75	0.4082372580517344
1106	76-77	0.43056723839198696
1106	78-79	0.4306676756389436
1106	80-81	0.35240560216933403
1106	82-83	0.3036428219856404
1106	84-85	0.31184793695592816
1106	86-87	0.3940450636263968
1106	88-89	0.3837964165349774
1106	90-91	0.31367236461847625
1106	92-93	0.33535939638662526
1106	94-95	0.3034395905462901
1106	96-97	0.335762048040543
1106	98-99	0.3468989003051455
1106	100	0.40698193340988453
1107	1	0.1750766729845452
1107	2	0.22087379095276916
1107	3	0.28804976561808715
1107	4	0.249968151965966
1107	5	0.21322093304346623
1107	6	0.1959850332581965
1107	7	0.16985436971688017
1107	8	0.22099205423239
1107	9	0.2064429407086621
1107	10-11	0.19905580855055405
1107	12-13	0.1965220385663642
1107	14-15	0.20088575492859917
1107	16-17	0.18318805594996945
1107	18-19	0.210028370636671
1107	20-21	0.26210886672026135
1107	22-23	0.25296472853167273
1107	24-25	0.22977766567662172
1107	26-27	0.21937887905387043
1107	28-29	0.2481432468248883
1107	30-31	0.24363097593486316
1107	32-33	0.28787668854154447
1107	34-35	0.3007287451824254
1107	36-37	0.3347881522052347
1107	38-39	0.26532189554170316
1107	40-41	0.2903437094570833
1107	42-43	0.2551832347514065
1107	44-45	0.22718431967353325
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2216	90-91	-1.6064189714895676
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2216	94-95	-1.969365509604927
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2216	98-99	-2.0321185676375855
2216	100	-2.0965168219467394
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	209.0
3	22.0
4	22.0
5	96.0
6	267.0
7	783.0
8	1686.0
9	2774.0
10	4278.0
11	6535.0
12	9385.0
13	12792.0
14	14272.0
15	17204.0
16	21070.0
17	28522.0
18	28174.0
19	16104.0
20	11958.0
21	11902.0
22	12259.0
23	13419.0
24	15753.0
25	19205.0
26	24157.0
27	30529.0
28	40304.0
29	52281.0
30	67968.0
31	87706.0
32	112809.0
33	147068.0
34	199246.0
35	288192.0
36	466748.0
37	846385.0
38	1086306.0
39	406847.0
40	1335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.309207645752984	20.604592334608533	14.06658614726863	23.019613872369852
2	16.97595628069326	20.856453855184423	38.24757503906554	23.920014825056782
3	17.999347874418827	25.187047782687795	30.90459168975771	25.909012653135672
4	13.289587144120338	17.940715917210873	37.30827825443343	31.461418684235355
5	12.495753682151147	36.38057625242329	34.71248866735387	16.41118139807169
6	29.889903730600558	37.86789567848217	17.636808209040456	14.605392381876817
7	26.048173513090724	32.52362310949376	22.702268461383362	18.725934916032156
8	24.818656533965555	34.573995049885895	21.274800490530787	19.332547925617767
9	24.333215148790767	16.624157569866057	22.62811902482168	36.4145082565215
10-11	23.453259799170695	26.451940937599534	28.773317501799557	21.321481761430213
12-13	24.769719648061564	24.08208990932945	28.93912329034653	22.209067152262456
14-15	22.21585843761133	26.360557262981693	26.984715471862046	24.438868827544926
16-17	20.73057528274191	29.28078455704661	27.515126972082797	22.47351318812869
18-19	20.376326045178313	27.136830913959383	30.934670084927284	21.552172955935024
20-21	21.859314776412052	26.903619369147798	30.421626602431417	20.81543925200873
22-23	21.397871509375705	27.060550746462013	30.463206294690558	21.078371449471724
24-25	21.1838243673799	26.770028627283292	30.756187886149323	21.289959119187486
26-27	21.00489167120411	26.491621235424585	30.898812927181112	21.604674166190193
28-29	21.11186532304656	26.604566952813997	30.880826870616396	21.402740853523046
30-31	21.724347099995786	27.274292394881073	30.187641100355698	20.813719404767443
32-33	20.92543367071124	26.941522028592217	30.850147032610163	21.282897268086376
34-35	20.962581442624163	26.203862978659576	30.902830876945544	21.930724701770725
36-37	21.907919792956264	26.987351007117372	29.935953880755044	21.168775319171317
38-39	21.671031215329965	26.919605938968072	30.20003058512063	21.20933226058133
40-41	21.221702675613628	26.646884554806295	30.470718643189503	21.660694126390574
42-43	21.18498104988784	26.789594825075515	30.166888587366785	21.858535537669862
44-45	21.6469478679541	27.00281402590774	29.90186218578415	21.448375920354007
46-47	21.419824125815886	27.626765097507118	29.295991888124696	21.6574188885523
48-49	21.32677814975605	27.010862101041937	29.609416320960648	22.052943428241363
50-51	21.403338356176395	26.807200750406906	29.44200174744288	22.34745914597382
52-53	21.29957784741142	27.06310762358483	29.301945759139254	22.3353687698645
54-55	21.50013441868303	27.659809690418186	28.944482161764117	21.895573729134664
56-57	20.887871928216526	27.348503812912572	29.004495720518232	22.75912853835267
58-59	20.737684209501	26.6828196541272	29.55600997670195	23.023486159669844
60-61	21.411483836153366	27.15206259624816	28.87610396213679	22.560349605461685
62-63	21.14179417772293	27.11194397663063	29.00361907693327	22.742642768713175
64-65	20.989903501022265	26.770004276072594	29.315752895602465	22.924339327302675
66-67	20.912405772990223	26.887547570090092	28.880061033874483	23.319985623045206
68-69	21.556130027672715	27.16763519548665	28.50673262273254	22.7695021541081
70-71	21.535894171586424	26.7054370409188	28.656200353969197	23.102468433525576
72-73	21.063395454895225	26.96560537596808	28.952725046583865	23.018274122552825
74-75	21.102880943034727	26.92954123293102	28.689975483201074	23.27760234083318
76-77	21.21367895169012	27.208253014923393	28.170832022426488	23.40723601095999
78-79	21.32653884942567	27.394369481171193	28.165585532341918	23.113506137061215
80-81	21.078740657928282	27.134117461415975	28.265405788839654	23.521736091816088
82-83	20.834756868338253	27.148981836626803	28.372414290205356	23.64384700482959
84-85	21.0067910656382	27.046767961209493	28.113399692005885	23.833041281146418
86-87	21.216235828812938	27.284033982601546	27.918017753006648	23.581712435578872
88-89	20.885778263341216	27.166008026894467	27.989067273791424	23.959146435972897
90-91	20.665940431774118	26.9599788728716	27.907899259442527	24.46618143591176
92-93	20.934206931281842	27.1589088824612	27.596162415969445	24.310721770287508
94-95	20.869110058563823	26.89004663408697	27.808361066497376	24.43248224085183
96-97	20.701244249461595	26.902158296506183	27.904551533493144	24.492045920539077
98-99	20.694577057111637	26.933955360810756	27.822287743229086	24.54917983884852
100	20.883170722085502	27.045757943096373	27.49219412437094	24.578877210447185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	599.0
1	562.5
2	1754.5
3	2732.0
4	1624.0
5	788.0
6	1355.5
7	2938.0
8	4661.5
9	5420.0
10	4469.5
11	3069.0
12	2775.0
13	3078.5
14	3518.5
15	3895.5
16	4307.5
17	4873.5
18	5476.5
19	6046.5
20	7000.0
21	8450.5
22	10508.5
23	13692.0
24	17495.0
25	21929.0
26	28127.5
27	35887.5
28	46170.0
29	57274.5
30	66337.5
31	75217.0
32	84219.0
33	92831.5
34	101364.5
35	109065.0
36	115548.5
37	121171.5
38	126176.5
39	129950.0
40	132842.0
41	133915.0
42	133340.0
43	133026.0
44	133075.5
45	133236.5
46	133074.0
47	132460.0
48	132117.0
49	132853.5
50	131736.0
51	128028.5
52	123495.0
53	121020.5
54	119519.5
55	116056.0
56	110046.0
57	100297.0
58	90339.0
59	81016.0
60	69961.0
61	57803.0
62	46710.0
63	37263.0
64	28668.0
65	21560.0
66	16425.0
67	12301.5
68	8943.0
69	6559.5
70	4734.0
71	3407.0
72	2612.0
73	1932.0
74	1341.0
75	936.5
76	624.5
77	425.0
78	300.5
79	216.5
80	136.0
81	60.0
82	33.0
83	26.5
84	15.0
85	6.0
86	3.0
87	2.5
88	1.5
89	1.5
90	1.5
91	1.5
92	1.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05807763750398143
2	2.6786331762842586E-4
3	2.435121069349326E-5
4	0.11515687536952962
5	0.0017776383806250079
6	0.0018263408020119944
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.652681604023989E-5
14-15	2.435121069349326E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.0958044812071968E-4
30-31	2.1916089624143936E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.0958044812071968E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.3393165881421293E-4
80-81	3.6526816040239886E-4
82-83	2.6786331762842586E-4
84-85	0.0
86-87	0.0
88-89	6.087802673373315E-5
90-91	8.52292374272264E-5
92-93	7.305363208047977E-4
94-95	6.453070833775714E-4
96-97	0.0
98-99	2.435121069349326E-5
100	0.001217560534674663
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4106572.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.8133287391175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72485614574063	53.12771494937597
2	12.025895385225875	15.82928412740213
3	3.285798973411929	6.487481040234422
4	1.3636558547885909	3.5898692423289527
5	0.7163700993261193	2.357335042291207
6	0.43962121545109156	1.7359761343903855
7	0.27817098267288837	1.281515082983391
8	0.20308442526416173	1.0692529633364012
9	0.1501779582581884	0.8895340202594056
>10	0.7592880464045763	8.622622619563789
>50	0.035622009834293006	1.596965919099461
>100	0.016496809542136837	1.9108237136039257
>500	5.55054325126868E-4	0.26379509644810395
>1k	2.960289123679393E-4	0.4321251354336504
>5k	3.700361404598867E-5	0.17548995299451825
>10k+	7.400722809197734E-5	0.6302149602542525
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13041	0.3175641386538456	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12837	0.31259649167237297	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7206	0.17547482425731242	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.435121069349326E-5	0.0
8	0.0	0.0	0.0	2.435121069349326E-5	0.0
9	0.0	0.0	0.0	7.305363208047977E-5	0.0
10-11	0.0	0.0	0.0	8.52292374272264E-5	0.0
12-13	0.0	0.0	0.0	1.0958044812071967E-4	0.0
14-15	0.0	0.0	0.0	1.217560534674663E-4	0.0
16-17	0.0	0.0	0.0	1.217560534674663E-4	0.0
18-19	0.0	0.0	0.0	1.217560534674663E-4	0.0
20-21	0.0	2.435121069349326E-5	0.0	2.8003892297517245E-4	0.0
22-23	0.0	4.870242138698652E-5	0.0	8.035899528852776E-4	0.0
24-25	0.0	4.870242138698652E-5	0.0	0.0020089748822131937	0.0
26-27	0.0	4.870242138698652E-5	0.0	0.004504973978296253	0.0
28-29	0.0	4.870242138698652E-5	0.0	0.012504346691108788	0.0
30-31	0.0	4.870242138698652E-5	0.0	0.036405059986772424	0.0
32-33	0.0	4.870242138698652E-5	0.0	0.09464098036026156	0.0
34-35	0.0	4.870242138698652E-5	0.0	0.19080391138886643	0.0
36-37	0.0	4.870242138698652E-5	0.0	0.3398941988597789	0.0
38-39	0.0	4.870242138698652E-5	0.0	0.5510070199670187	0.0
40-41	0.0	4.870242138698652E-5	0.0	0.7994989494887708	0.0
42-43	0.0	4.870242138698652E-5	0.0	1.0563189930676975	0.0
44-45	0.0	4.870242138698652E-5	0.0	1.3602951561545735	0.0
46-47	0.0	4.870242138698652E-5	0.0	1.7226168200630598	0.0
48-49	0.0	4.870242138698652E-5	0.0	2.108120836551752	0.0
50-51	0.0	7.305363208047977E-5	0.0	2.507115423764639	0.0
52-53	0.0	7.305363208047977E-5	0.0	2.8987194185320506	0.0
54-55	0.0	7.305363208047977E-5	0.0	3.342422828578191	0.0
56-57	0.0	7.305363208047977E-5	0.0	3.852398058526674	0.0
58-59	0.0	7.305363208047977E-5	0.0	4.395758798335936	0.0
60-61	0.0	7.305363208047977E-5	0.0	4.9470458572259295	0.0
62-63	0.0	7.305363208047977E-5	0.0	5.483868296963989	0.0
64-65	0.0	7.305363208047977E-5	0.0	6.074165995384959	0.0
66-67	0.0	7.305363208047977E-5	0.0	6.747403917427967	0.0
68-69	0.0	7.305363208047977E-5	0.0	7.446697634913012	0.0
70-71	0.0	7.305363208047977E-5	0.0	8.168504046684193	0.0
72-73	0.0	9.740484277397304E-5	0.0	8.939000704237014	0.0
74-75	0.0	9.740484277397304E-5	0.0	9.688944939964525	0.0
76-77	0.0	9.740484277397304E-5	0.0	10.465115429608929	0.0
78-79	0.0	9.740484277397304E-5	0.0	11.303113643204114	0.0
80-81	0.0	9.740484277397304E-5	0.0	12.178490965213808	0.0
82-83	0.0	9.740484277397304E-5	0.0	13.058348909991107	0.0
84-85	0.0	9.740484277397304E-5	0.0	13.896140138295396	0.0
86-87	0.0	9.740484277397304E-5	0.0	14.761947434502549	0.0
88	0.0	1.217560534674663E-4	0.0	15.448091498213108	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7625	0.0	59.539223	1
GTATCAA	13805	0.0	39.981327	1
GTGGTAT	3005	0.0	34.321453	1
ATCAACG	16965	0.0	32.540886	3
ATAACGC	395	0.0	32.170063	3
TCAACGC	17320	0.0	32.064125	4
TATCAAC	17440	0.0	31.708569	2
CAACGCA	17835	0.0	31.174456	5
TGGTATC	3245	0.0	30.022087	2
TATAACG	410	0.0	29.845215	2
AACGCAG	18895	0.0	29.574108	6
ACGCAGA	21415	0.0	25.873898	7
CGCAGAG	21380	0.0	25.69644	8
GCAGAGT	23260	0.0	23.1548	9
GCGGTAT	190	3.5749872E-7	22.307707	1
GAGTACT	17695	0.0	21.765293	12-13
GTACATG	9200	0.0	21.653023	1
CAGAGTA	22670	0.0	21.26973	10-11
GTCTCGC	1440	0.0	20.276602	1
AGAGTAC	21630	0.0	19.945837	10-11
>>END_MODULE