FastQCFastQC Report
Wed 25 May 2016
SRR2049509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864589
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35800.043.772651
GTATCAA58350.033.701931
TCAACGC60000.031.1470554
ATCAACG59850.031.139573
ATAACGC3500.030.7203833
GTCTCGC15350.030.3596971
GTACCTG30250.029.6262781
TACCTGG29800.029.208422
TCGCTCT15500.029.0687484
CAACGCA64400.029.018575
TATCAAC69950.027.3753222
TCTGTCG16350.027.1119678
CTCGCTC15450.026.8430543
CGCTCTG15250.026.5232225
TATAACG4250.026.503862
AACGCAG73050.025.9328676
TCTCGCT16800.025.2955552
GTACATG75600.025.0636521
GTGGTAT21250.023.1353861
CGCAGAG79550.023.085468
Produced by FastQC (version 0.11.5)