##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049509_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3864589 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.43726072811365 31.0 31.0 34.0 27.0 34.0 2 30.645025641795286 31.0 31.0 34.0 27.0 34.0 3 30.734551850144996 31.0 31.0 34.0 27.0 34.0 4 34.27180924025815 37.0 35.0 37.0 30.0 37.0 5 34.275309224344426 37.0 35.0 37.0 30.0 37.0 6 34.30588815524756 37.0 35.0 37.0 30.0 37.0 7 30.39144007292884 37.0 32.0 37.0 2.0 37.0 8 32.12255015992645 37.0 32.0 37.0 17.0 37.0 9 34.80446277728369 39.0 33.0 39.0 27.0 39.0 10-11 35.32026846839341 39.0 35.0 39.0 29.0 39.0 12-13 35.45081042253135 39.0 35.0 39.0 29.5 39.0 14-15 36.593873371786756 40.0 37.0 41.0 29.0 41.0 16-17 36.60325198876258 40.0 37.0 41.0 29.0 41.0 18-19 36.61170942110532 40.0 37.0 41.0 29.0 41.0 20-21 36.6869716288071 40.0 37.0 41.0 30.0 41.0 22-23 36.64916786752744 40.0 37.0 41.0 30.0 41.0 24-25 36.631230772534934 40.0 37.0 41.0 30.0 41.0 26-27 36.660205910641466 40.0 37.0 41.0 30.0 41.0 28-29 36.637297264987296 40.0 37.0 41.0 30.0 41.0 30-31 36.55203735765951 40.0 36.5 41.0 30.0 41.0 32-33 36.48312860694889 40.0 36.0 41.0 30.0 41.0 34-35 36.421481818635826 40.0 36.0 41.0 30.0 41.0 36-37 36.23069400135435 40.0 36.0 41.0 29.0 41.0 38-39 36.0873363247683 40.0 36.0 41.0 28.5 41.0 40-41 35.99214922984048 40.0 35.5 41.0 28.5 41.0 42-43 35.86333320309093 40.0 35.0 41.0 28.0 41.0 44-45 35.732382020442536 40.0 35.0 41.0 27.5 41.0 46-47 35.558766275016566 39.0 35.0 41.0 27.0 41.0 48-49 35.452264264065334 39.0 35.0 41.0 27.0 41.0 50-51 35.312434258856506 39.0 35.0 41.0 26.0 41.0 52-53 35.15810736924418 39.0 35.0 41.0 26.0 41.0 54-55 35.00930603487201 39.0 34.0 41.0 26.0 41.0 56-57 34.803627759640165 39.0 34.0 40.5 26.0 41.0 58-59 34.61091321742105 38.0 34.0 40.0 25.0 41.0 60-61 34.350032176772224 38.0 34.0 40.0 24.5 41.0 62-63 34.05293111376139 37.5 33.5 40.0 24.0 41.0 64-65 33.64524028298999 37.0 33.0 40.0 22.5 41.0 66-67 30.250990855689956 35.5 29.0 39.0 6.5 41.0 68-69 30.549823150663627 35.0 28.5 39.0 7.0 41.0 70-71 30.658071401641934 35.0 28.0 39.0 11.0 40.5 72-73 31.81063018603013 35.0 30.5 38.0 17.5 40.0 74-75 31.894524100751724 35.0 32.0 37.0 17.5 40.0 76-77 31.6125001908353 35.0 32.0 37.0 14.5 39.0 78-79 28.396379666764048 35.0 27.0 36.0 4.5 39.0 80-81 30.084413116116615 35.0 28.5 36.0 7.0 38.5 82-83 30.483502644136287 35.0 31.0 35.5 3.5 37.0 84-85 28.720433660604016 34.5 28.5 35.0 2.0 37.0 86-87 28.34558021564518 34.0 25.5 35.0 2.0 36.5 88-89 29.53328439324337 34.0 30.0 35.0 2.0 36.0 90-91 29.678163965171976 34.0 30.5 35.0 2.0 36.0 92-93 29.51821552046026 34.0 31.0 35.0 2.0 36.0 94-95 29.29011726732131 34.0 30.5 35.0 2.0 35.5 96-97 29.080321477911365 34.0 30.0 35.0 2.0 35.0 98-99 28.87323322091948 34.0 30.0 35.0 2.0 35.0 100 28.652850794741692 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42571798026603247 1101 2 0.4052632779516152 1101 3 0.37460630630611647 1101 4 0.4909286037242566 1101 5 0.3882955634762766 1101 6 0.41905844832051997 1101 7 -11.958351149322418 1101 8 -6.380146667707955 1101 9 -2.1196210456148563 1101 10-11 -0.07281530251732704 1101 12-13 0.40624873091546476 1101 14-15 0.6117198710311769 1101 16-17 0.7109601029597385 1101 18-19 0.6949817781507832 1101 20-21 0.6655079778621129 1101 22-23 0.6259624739335408 1101 24-25 0.6721906226384888 1101 26-27 0.6192739222404171 1101 28-29 0.555976636256446 1101 30-31 0.6159658425298389 1101 32-33 0.6435610296060119 1101 34-35 0.6517454383927017 1101 36-37 0.6995341397800843 1101 38-39 0.6626977015173594 1101 40-41 0.6406991623314724 1101 42-43 0.6469152192113299 1101 44-45 0.5884719389683823 1101 46-47 0.557445294308188 1101 48-49 0.4797859467524148 1101 50-51 0.5423059971973032 1101 52-53 0.641781910457226 1101 54-55 0.5485024717510214 1101 56-57 0.5003897931491608 1101 58-59 0.42756230534759965 1101 60-61 0.5017734450815752 1101 62-63 0.47060833565516447 1101 64-65 0.32611043144338225 1101 66-67 -9.983658025462447 1101 68-69 -7.96403841153144 1101 70-71 -6.549830966662718 1101 72-73 -1.6317697293166553 1101 74-75 -0.29608337242310157 1101 76-77 0.04469866863212246 1101 78-79 3.0947934576500984 1101 80-81 1.093821814590246 1101 82-83 0.3795920442953147 1101 84-85 -5.258982352256563 1101 86-87 -5.708439496908568 1101 88-89 -1.2521305549812922 1101 90-91 0.011830901294917595 1101 92-93 0.32261500003556165 1101 94-95 0.4045923189471736 1101 96-97 0.5321250808214799 1101 98-99 0.522040929561669 1101 100 0.5283778776703585 1102 1 0.20616448738508097 1102 2 0.2769286282902961 1102 3 0.20611800442492978 1102 4 0.3092353810137709 1102 5 0.2562482122801484 1102 6 0.3417851096528963 1102 7 -11.894689170410548 1102 8 -6.4831381789179225 1102 9 -2.2510668632203448 1102 10-11 -0.24187966923135207 1102 12-13 0.28794457677535945 1102 14-15 0.4624814399491086 1102 16-17 0.4716895301726822 1102 18-19 0.5308657456738644 1102 20-21 0.4291565292999806 1102 22-23 0.3415529883069439 1102 24-25 0.40816761745241337 1102 26-27 0.3757456894591513 1102 28-29 0.3732296602734806 1102 30-31 0.3597298768765782 1102 32-33 0.4029922465828548 1102 34-35 0.34146476870232334 1102 36-37 0.5165181168804907 1102 38-39 0.5180171003007956 1102 40-41 0.47482537103627465 1102 42-43 0.4470204237519866 1102 44-45 0.43638531813133596 1102 46-47 0.3642662587569845 1102 48-49 0.2154504518466993 1102 50-51 0.2881645715729775 1102 52-53 0.20940777984387893 1102 54-55 0.16946033448714815 1102 56-57 0.20967755270498856 1102 58-59 0.19316017846755784 1102 60-61 0.07093770371256625 1102 62-63 0.07213189465407055 1102 64-65 -0.02746800530017879 1102 66-67 -10.008904694361167 1102 68-69 -6.563110635755201 1102 70-71 -6.121497597071777 1102 72-73 -1.2266536196375775 1102 74-75 -0.3519272385911485 1102 76-77 -0.22554632423662468 1102 78-79 2.7947233555575224 1102 80-81 0.8532681038983547 1102 82-83 0.2305821522032545 1102 84-85 -5.360183903914098 1102 86-87 -5.9161582417020675 1102 88-89 -1.5805189642822732 1102 90-91 -0.3259329125878878 1102 92-93 0.03610847061543154 1102 94-95 -0.01409072939252809 1102 96-97 0.06984381367649917 1102 98-99 0.10205192755493897 1102 100 0.17927267339500474 1103 1 0.14742983984401192 1103 2 0.16357979858195293 1103 3 0.13131025936971596 1103 4 0.1617557112757666 1103 5 0.1841024490000791 1103 6 0.2225935201083118 1103 7 -12.061838146405346 1103 8 -6.563259411279738 1103 9 -2.447334209340198 1103 10-11 -0.4223996337772036 1103 12-13 0.0776169370159181 1103 14-15 0.2421217174870236 1103 16-17 0.2982900144926006 1103 18-19 0.3802935522912989 1103 20-21 0.31953670265959744 1103 22-23 0.26291614443725564 1103 24-25 0.3075899637246451 1103 26-27 0.3122680019534343 1103 28-29 0.31251579633004667 1103 30-31 0.26247179556568767 1103 32-33 0.2647934811288053 1103 34-35 0.2598832114626717 1103 36-37 0.31900070091901256 1103 38-39 0.34364043680028544 1103 40-41 0.34678240319897213 1103 42-43 0.34121810856562007 1103 44-45 0.30448026718504195 1103 46-47 0.30790794074648176 1103 48-49 0.25395648870276233 1103 50-51 0.2788312543243805 1103 52-53 0.24229940722992893 1103 54-55 0.265845776444948 1103 56-57 0.2408766318114246 1103 58-59 0.18857612343743568 1103 60-61 0.17169224722770338 1103 62-63 0.09879866502496526 1103 64-65 -0.05401769571130899 1103 66-67 -10.193686521824418 1103 68-69 -8.188165524922894 1103 70-71 -6.782306413878466 1103 72-73 -1.8353219176352802 1103 74-75 -0.5693912638052474 1103 76-77 -0.2672403455395873 1103 78-79 2.709141111330247 1103 80-81 0.7089095928709952 1103 82-83 0.029741608251285356 1103 84-85 -5.547629268715955 1103 86-87 -6.01834199882591 1103 88-89 -1.6460926624343912 1103 90-91 -0.42690371116972514 1103 92-93 -0.02330398989230531 1103 94-95 0.07721522644725098 1103 96-97 0.18444839579500893 1103 98-99 0.18059651698777657 1103 100 0.2037415595861738 1104 1 0.14938261035533884 1104 2 0.15638404266131545 1104 3 0.173544067556751 1104 4 0.1447805440800778 1104 5 0.11563288808891059 1104 6 0.2014689577351092 1104 7 -12.169942906828325 1104 8 -6.633133313012809 1104 9 -2.434774630648505 1104 10-11 -0.35778509345863085 1104 12-13 0.14964569098052038 1104 14-15 0.38930071600198346 1104 16-17 0.423090369497757 1104 18-19 0.34179011333021236 1104 20-21 0.36455034529459596 1104 22-23 0.43042233614124825 1104 24-25 0.4479501874069527 1104 26-27 0.4044063994780913 1104 28-29 0.3407948870470605 1104 30-31 0.36818553815184174 1104 32-33 0.35557403236754226 1104 34-35 0.34489075168983163 1104 36-37 0.43334508255681925 1104 38-39 0.4642741427962065 1104 40-41 0.4772406831311926 1104 42-43 0.4177514444819863 1104 44-45 0.35314660653340724 1104 46-47 0.4589049397905569 1104 48-49 0.4167869828222237 1104 50-51 0.4282371171158559 1104 52-53 0.41212172908713995 1104 54-55 0.42429070978158734 1104 56-57 0.361744989918634 1104 58-59 0.3829118418355293 1104 60-61 0.3137767340957325 1104 62-63 0.2527367764387165 1104 64-65 0.18125104331208064 1104 66-67 -10.13579013925672 1104 68-69 -8.146445638761858 1104 70-71 -6.610239186896138 1104 72-73 -1.7103304991463162 1104 74-75 -0.4751908820415167 1104 76-77 -0.12284862786313155 1104 78-79 2.8777610530062425 1104 80-81 0.8758891491993097 1104 82-83 0.20197389689033685 1104 84-85 -5.377801498280302 1104 86-87 -5.905837420506533 1104 88-89 -1.4653021402971191 1104 90-91 -0.13681310131033797 1104 92-93 0.19628607117414987 1104 94-95 0.2780906011126909 1104 96-97 0.28622992959405735 1104 98-99 0.32492263983384717 1104 100 0.3716123822670312 1105 1 0.2878348734732228 1105 2 0.28729584494354654 1105 3 0.2809788614771982 1105 4 0.35813562125181164 1105 5 0.3279878649715471 1105 6 0.3792360864583415 1105 7 -11.984915719763002 1105 8 -6.334402507836732 1105 9 -2.1427300848490916 1105 10-11 -0.18998114846356629 1105 12-13 0.49707241816946635 1105 14-15 0.5619565747019166 1105 16-17 0.5521221873029987 1105 18-19 0.5864364634612329 1105 20-21 0.605046035043749 1105 22-23 0.6186678147745894 1105 24-25 0.5960359011528098 1105 26-27 0.5339558398213384 1105 28-29 0.5440788316351615 1105 30-31 0.5202077005882018 1105 32-33 0.5826639771503963 1105 34-35 0.5839405924627243 1105 36-37 0.6820592164878221 1105 38-39 0.7051620937155931 1105 40-41 0.671564276794669 1105 42-43 0.6240143385345505 1105 44-45 0.5800874839321679 1105 46-47 0.5818753719493444 1105 48-49 0.6237688770070591 1105 50-51 0.7436608094696169 1105 52-53 0.7510354383156326 1105 54-55 0.6793556724057339 1105 56-57 0.6229630518391573 1105 58-59 0.6206779676678238 1105 60-61 0.6179020460456783 1105 62-63 0.5198188870445009 1105 64-65 0.3574874988338692 1105 66-67 -9.867069464732623 1105 68-69 -7.875689124694496 1105 70-71 -6.359521974525986 1105 72-73 -1.431862669416848 1105 74-75 -0.1740523391984503 1105 76-77 0.11911205299807648 1105 78-79 3.139408439457913 1105 80-81 1.1917999994618782 1105 82-83 0.4892243042239386 1105 84-85 -5.142642060422478 1105 86-87 -5.624423954733455 1105 88-89 -1.1970327230930309 1105 90-91 0.1249550535592796 1105 92-93 0.4193715160289564 1105 94-95 0.5490963117771983 1105 96-97 0.5486710678898064 1105 98-99 0.6570027519250772 1105 100 0.7375297585880496 1106 1 0.03832511848907316 1106 2 0.07573713798557691 1106 3 -0.023578697835581863 1106 4 -0.06565399703883656 1106 5 -0.07772937061142926 1106 6 -0.0036747513226558226 1106 7 -11.970163231420774 1106 8 -6.552805105183921 1106 9 -2.5940156607835263 1106 10-11 -0.5682698772225336 1106 12-13 -0.014327580977258947 1106 14-15 0.13333481551968873 1106 16-17 0.08937146522512052 1106 18-19 0.18226402657105467 1106 20-21 0.19474329562746817 1106 22-23 0.21616334578447294 1106 24-25 0.24019528304561533 1106 26-27 0.1696607456845669 1106 28-29 0.20608059789951483 1106 30-31 0.15650567023746476 1106 32-33 0.179370406096659 1106 34-35 0.20255885324696266 1106 36-37 0.22145872927189458 1106 38-39 0.19326421280618433 1106 40-41 0.2045824983285911 1106 42-43 0.21561233847481986 1106 44-45 0.15105095127120904 1106 46-47 0.2255683149979859 1106 48-49 0.21792522979916384 1106 50-51 0.22183055003353758 1106 52-53 0.20136808476838297 1106 54-55 0.20131878563864092 1106 56-57 0.2036130712509987 1106 58-59 0.17508347465821572 1106 60-61 0.1596459121167655 1106 62-63 0.07758046695516185 1106 64-65 -0.05353719046999572 1106 66-67 -9.96383383056266 1106 68-69 -8.042170120287366 1106 70-71 -6.523146101526365 1106 72-73 -1.7612937531096229 1106 74-75 -0.6079878236199114 1106 76-77 -0.2602430471968944 1106 78-79 2.7124763638413185 1106 80-81 0.7499616339816804 1106 82-83 0.08869945919870403 1106 84-85 -5.362243925665894 1106 86-87 -5.893106381256434 1106 88-89 -1.676982124312353 1106 90-91 -0.44865021622267065 1106 92-93 -0.062102418772852275 1106 94-95 -0.04759313713978841 1106 96-97 0.04338264204091047 1106 98-99 0.0633203040704089 1106 100 0.13478846511938514 1107 1 -0.07103769701382134 1107 2 -0.008040956952356737 1107 3 -0.06220141786571887 1107 4 -0.10889597787008398 1107 5 -0.08042547369803543 1107 6 -0.12843147633514462 1107 7 -12.250861629464431 1107 8 -6.809362824714995 1107 9 -2.723000431572636 1107 10-11 -0.6750512565924822 1107 12-13 -0.15678586527620553 1107 14-15 -0.06237376432458319 1107 16-17 -0.032690537558131894 1107 18-19 0.03611970393404107 1107 20-21 0.006093895759228474 1107 22-23 0.038925016442235005 1107 24-25 0.20420867552093114 1107 26-27 0.08994652792519986 1107 28-29 0.0779022199165027 1107 30-31 0.07423462071277243 1107 32-33 0.10893428056930077 1107 34-35 0.11993846075652925 1107 36-37 0.1429545470594249 1107 38-39 0.17237990282171722 1107 40-41 0.1268309670693526 1107 42-43 0.15158397694772674 1107 44-45 0.08779917555837358 1107 46-47 0.08657796331341672 1107 48-49 0.10978040023406521 1107 50-51 0.10403660999443787 1107 52-53 0.08872373844756964 1107 54-55 0.1043621506413075 1107 56-57 0.013947932231019422 1107 58-59 0.04961181423107064 1107 60-61 0.08631492373030625 1107 62-63 0.02965605458529552 1107 64-65 -0.1502536539218795 1107 66-67 -10.222762426400323 1107 68-69 -8.287793346408705 1107 70-71 -6.802167343687628 1107 72-73 -1.9814572890239361 1107 74-75 -0.7018510312584034 1107 76-77 -0.4227648821783596 1107 78-79 2.5880256172940364 1107 80-81 0.6042264217227036 1107 82-83 -0.08796810181720005 1107 84-85 -5.612673260722538 1107 86-87 -6.116890915732032 1107 88-89 -1.796754819010033 1107 90-91 -0.5549405822402527 1107 92-93 -0.1761276503565803 1107 94-95 -0.1422554148559243 1107 96-97 -0.06510252004691353 1107 98-99 -0.03730376256067558 1107 100 0.055149120515022076 1108 1 0.0014844452207718462 1108 2 8.853227011158538E-4 1108 3 0.047495582878230636 1108 4 0.06141467547504931 1108 5 -0.034099697600495915 1108 6 0.06288567889940566 1108 7 -12.226175541426223 1108 8 -6.697873970434223 1108 9 -2.6679020583696555 1108 10-11 -0.5829831784981465 1108 12-13 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1.3127357495864729 2216 9 0.16541451098189697 2216 10-11 -0.4832884590083779 2216 12-13 -0.8017548214942138 2216 14-15 -0.9572430386737949 2216 16-17 -1.125827960469266 2216 18-19 -1.6069299559889387 2216 20-21 -1.2080599086850796 2216 22-23 -1.028549419746497 2216 24-25 -1.0221976058723072 2216 26-27 -1.4960894109164897 2216 28-29 -1.7199307017927623 2216 30-31 -1.7069165540964235 2216 32-33 -2.617955778276695 2216 34-35 -1.6670306564865527 2216 36-37 -1.3550498169308156 2216 38-39 -1.625475733092422 2216 40-41 -1.6005981962789804 2216 42-43 -1.2761383697974935 2216 44-45 -1.4110687333457221 2216 46-47 -1.5915718007572934 2216 48-49 -1.6399925725035658 2216 50-51 -2.1208778126856913 2216 52-53 -1.8252343808259894 2216 54-55 -1.4435637953249767 2216 56-57 -1.4048418846425221 2216 58-59 -1.7613433007985506 2216 60-61 -1.876351998329369 2216 62-63 -1.7753086142927756 2216 64-65 -1.4795111649165946 2216 66-67 1.803590089038515 2216 68-69 0.9026281922320862 2216 70-71 0.5478963789750111 2216 72-73 -1.1036359572309564 2216 74-75 -1.9123824196832189 2216 76-77 -1.583440576026419 2216 78-79 0.7998633425849846 2216 80-81 -0.536082881467344 2216 82-83 -1.3942847655439827 2216 84-85 0.3430822095453685 2216 86-87 0.4274288058506954 2216 88-89 -0.8240432623223839 2216 90-91 -1.1862490373883041 2216 92-93 -1.1957647421831936 2216 94-95 -1.2557306263525874 2216 96-97 -1.1793945921493076 2216 98-99 -1.212932087838567 2216 100 -2.1951044291601747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82118.0 3 15006.0 4 8784.0 5 7968.0 6 7163.0 7 7755.0 8 8150.0 9 9223.0 10 10529.0 11 11718.0 12 13495.0 13 15131.0 14 17394.0 15 18747.0 16 19279.0 17 20013.0 18 18473.0 19 17430.0 20 17276.0 21 17253.0 22 18436.0 23 21395.0 24 26138.0 25 31909.0 26 39097.0 27 46670.0 28 56346.0 29 71110.0 30 85718.0 31 102056.0 32 134693.0 33 187581.0 34 279537.0 35 364551.0 36 422643.0 37 701539.0 38 707400.0 39 224254.0 40 611.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.433500251166095 19.456896353161525 12.000925471347268 23.108677924325118 2 18.17697458494856 20.768783505122627 37.406369379284 23.647872530644808 3 18.473172814428587 24.799860268336545 30.176424163227285 26.550542754007584 4 13.364234883870774 18.124346783607116 37.708560236675545 30.80285809584656 5 12.427927995532713 35.59633777698654 33.34693564922897 18.628798578251786 6 29.095942348731796 37.5090083341161 18.689540654531 14.7055086626211 7 26.237365239225362 31.94045264448754 22.54737513586276 19.274806980424337 8 24.312268215913313 33.839312237164734 22.23409067150237 19.614328875419577 9 23.542477608873803 18.4221659793577 23.02141314380391 35.013943267964585 10-11 22.857657049688854 26.774748880152586 28.637042645414557 21.730551424744 12-13 24.269256058018073 24.61419571395561 28.16637163744967 22.950176590576643 14-15 22.19530977291505 27.429087543332553 25.85227044842285 24.523332235329555 16-17 20.987124341357752 30.110459638682507 25.882314735964773 23.020101283994972 18-19 20.57270514406577 28.47315717143531 28.439233771042666 22.514903913456255 20-21 22.685701207256606 28.74707100122044 27.145438641138636 21.421789150384317 22-23 21.90621330058815 29.31503574054521 27.165854786350472 21.61289617251617 24-25 21.583873472703047 28.64642268556889 27.751062273374995 22.018641568353065 26-27 21.344660128228597 28.076202280582702 28.079139208439724 22.499998382748977 28-29 21.729891587436594 28.295039912394305 27.775553881667626 22.199514618501475 30-31 22.310020846980215 29.69668051333279 26.674457060564144 21.31884157912285 32-33 20.935126728240277 28.81275716677209 28.08958832227545 22.162527782712182 34-35 20.922592524740026 27.60413270444353 28.43921525399344 23.03405951682301 36-37 22.572680784171663 28.983080341692354 26.889454566625805 21.55478430751018 38-39 22.13199413081108 28.924243810175394 27.425882471057395 21.51787958795613 40-41 21.24694517928623 28.41662184731958 27.99904828159583 22.337384691798366 42-43 21.078145975715913 28.54132186923936 27.749698027318825 22.630834127725905 44-45 21.93290403313785 28.92293193782306 27.482544046137953 21.661619982901133 46-47 21.417976972769182 30.032332312517223 26.840709991189154 21.708980723524444 48-49 21.119041279398186 28.739946803872208 28.011831597175053 22.12918031955456 50-51 21.34511659030433 28.0075102608188 28.09735185936788 22.550021289508987 52-53 21.304402559500947 28.383694327574666 27.724023933768766 22.58787917915562 54-55 21.74056655647775 29.590562998518593 27.229501303506854 21.4393691414968 56-57 20.801766937561382 29.131543357235707 27.293006061912422 22.77368364329049 58-59 20.53564451280321 28.133679796345163 28.255336028927353 23.075339661924275 60-61 21.821831548086514 29.158296772323776 27.019894968230414 21.99997671135929 62-63 21.49343835756455 28.96421404552459 27.20043058554718 22.34191701136368 64-65 21.297051738766932 28.305256318395756 27.92676454613462 22.470927396702695 66-67 21.146697939014526 28.583163159884638 27.183010278549574 23.087128622551262 68-69 22.48505888845576 28.958450132055557 26.893781782027304 21.66270919746138 70-71 22.297963007600085 28.269737079591096 27.37139236637942 22.060907546429398 72-73 21.45805281838519 28.534371903338158 28.22621139438296 21.78136388389369 74-75 21.322340033251606 28.35227294781309 28.003803880216587 22.321583138718715 76-77 21.526660646720277 28.783615877908346 27.22270390131601 22.46701957405537 78-79 21.892646847829404 29.099956820423976 27.2990388748855 21.708357456861112 80-81 21.276224104212613 28.388543392486433 27.88109173269123 22.454140770609722 82-83 21.05590571617918 28.523332238730966 27.97912337100266 22.44163867408719 84-85 21.429392791899918 28.565324772261068 27.590155517933564 22.41512691790545 86-87 21.82799714009128 28.663895774285614 27.535264315134405 21.9728427704887 88-89 21.09878690152258 28.51308653546212 27.80301229737001 22.585114265645288 90-91 20.8555518034191 28.22719315610311 27.783794134505037 23.133460905972754 92-93 21.46047294311845 28.819624369300705 27.202982149772264 22.516920537808577 94-95 21.394068104722276 28.402525494697667 27.62966915439815 22.573737246181906 96-97 21.151647381414556 28.34259913005147 27.934433118647352 22.571320369886617 98-99 21.19094530978288 28.279565384806478 27.872864083900566 22.656625221510076 100 21.649249506001986 28.659880943631954 27.347072809459092 22.343796740906967 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4464.0 1 3464.5 2 3001.0 3 3389.5 4 2939.0 5 2450.5 6 2160.5 7 2049.5 8 2073.5 9 2066.0 10 1872.5 11 1661.5 12 1618.5 13 1660.5 14 1718.5 15 1855.0 16 2057.5 17 2465.0 18 3209.0 19 4031.0 20 5175.5 21 7139.5 22 10370.0 23 15124.5 24 20396.5 25 26175.0 26 33625.5 27 43015.5 28 54600.0 29 65382.0 30 73156.5 31 80587.0 32 88163.0 33 95237.5 34 102120.5 35 108021.5 36 111526.5 37 113848.5 38 115739.5 39 116040.0 40 116036.0 41 114620.5 42 111412.0 43 110255.5 44 110572.0 45 112013.0 46 114754.5 47 118169.0 48 122721.5 49 126688.0 50 128037.5 51 124570.0 52 118134.0 53 113122.0 54 110190.5 55 106903.0 56 101462.0 57 94740.5 58 87592.0 59 79244.0 60 68765.0 61 56960.0 62 45485.0 63 35339.0 64 27134.0 65 20439.5 66 15162.5 67 11055.5 68 7908.0 69 5731.0 70 4129.5 71 2997.0 72 2188.0 73 1660.5 74 1264.0 75 910.5 76 672.5 77 504.0 78 389.0 79 316.0 80 207.0 81 136.0 82 114.5 83 87.0 84 70.0 85 50.5 86 38.5 87 28.5 88 21.0 89 22.0 90 21.0 91 18.0 92 12.5 93 11.0 94 10.5 95 12.0 96 14.5 97 7.0 98 3.0 99 9.5 100 17.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.015836095377800848 2 0.001759566153088983 3 0.0010091629407422108 4 0.0015008064246935444 5 0.0013196746148167373 6 0.002898108958028913 7 11.651898817700925 8 2.587597283954387E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.587597283954387E-4 18-19 0.0 20-21 1.2937986419771935E-5 22-23 1.2937986419771935E-5 24-25 0.0 26-27 1.6819382345703514E-4 28-29 0.0 30-31 1.2937986419771935E-5 32-33 1.2937986419771935E-5 34-35 2.587597283954387E-5 36-37 3.88139592593158E-5 38-39 1.2937986419771935E-5 40-41 1.2937986419771935E-5 42-43 2.587597283954387E-5 44-45 1.2937986419771935E-4 46-47 8.797830765444915E-4 48-49 5.692714024699651E-4 50-51 2.4582174197566674E-4 52-53 1.9406979629657902E-4 54-55 3.6226361975361413E-4 56-57 0.0012808606555574215 58-59 2.8463570123498256E-4 60-61 0.0011385428049399302 62-63 0.002173581718521685 64-65 0.0010350389135817548 66-67 5.826363424415895 68-69 5.474864209363531 70-71 0.002393527487657808 72-73 0.0011514807913597022 74-75 0.002949860903708001 76-77 0.005524520201242616 78-79 5.406150563488123 80-81 0.004696489070377213 82-83 0.004450667328401545 84-85 5.826479866293673 86-87 0.003363876469140703 88-89 0.0016431242753110358 90-91 0.00320862063210344 92-93 0.0033250625098813873 94-95 0.004088403708647931 96-97 0.002781667080250966 98-99 0.005304574432106493 100 0.005201070540748318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3864589.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.06357454681749 #Duplication Level Percentage of deduplicated Percentage of total 1 94.67113026246247 87.15762658349682 2 3.9237960670403775 7.224773834489623 3 0.7798124047598554 2.153769523744259 4 0.27476788151911985 1.0118445337322644 5 0.13926156769167375 0.6410458859344538 6 0.07212059800465337 0.3983808030457477 7 0.038698367546396294 0.24938970318134582 8 0.02896911989021782 0.21336005828550092 9 0.015523526150621497 0.12862361762974917 >10 0.055179021150273115 0.7509666907775767 >50 6.192135653796722E-4 0.04221263291155807 >100 9.241756901336123E-5 0.010076570904264575 >500 2.9552649929137744E-5 0.01792956186682927 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.587597283954387E-5 0.0 5 0.0 0.0 0.0 2.587597283954387E-5 0.0 6 0.0 0.0 0.0 2.587597283954387E-5 0.0 7 0.0 0.0 0.0 2.587597283954387E-5 0.0 8 0.0 0.0 0.0 2.587597283954387E-5 0.0 9 2.587597283954387E-5 0.0 0.0 2.587597283954387E-5 0.0 10-11 2.587597283954387E-5 0.0 0.0 5.175194567908774E-5 0.0 12-13 2.587597283954387E-5 0.0 0.0 5.175194567908774E-5 0.0 14-15 2.587597283954387E-5 0.0 0.0 7.76279185186316E-5 0.0 16-17 2.587597283954387E-5 0.0 0.0 7.76279185186316E-5 0.0 18-19 3.88139592593158E-5 0.0 0.0 9.056590493840353E-5 0.0 20-21 5.175194567908774E-5 0.0 0.0 1.4231785061749128E-4 0.0 22-23 5.175194567908774E-5 0.0 0.0 3.8813959259315805E-4 0.0 24-25 5.175194567908774E-5 0.0 0.0 9.83286967902667E-4 0.0 26-27 5.175194567908774E-5 0.0 0.0 0.001759566153088983 0.0 28-29 5.175194567908774E-5 0.0 0.0 0.004528295246920177 0.0 30-31 5.175194567908774E-5 0.0 0.0 0.012899172460512618 0.0 32-33 5.175194567908774E-5 0.0 0.0 0.029666802860537043 0.0 34-35 5.175194567908774E-5 0.0 0.0 0.05705652011119423 0.0 36-37 5.175194567908774E-5 0.0 0.0 0.09402034731248265 0.0 38-39 5.175194567908774E-5 0.0 0.0 0.147894122764413 0.0 40-41 5.175194567908774E-5 0.0 0.0 0.21889779223612135 0.0 42-43 5.175194567908774E-5 0.0 0.0 0.2955941757325294 0.0 44-45 5.175194567908774E-5 0.0 0.0 0.3790183121672188 0.0 46-47 5.175194567908774E-5 0.0 0.0 0.47014055052167253 0.0 48-49 5.175194567908774E-5 0.0 0.0 0.572738782830464 0.0 50-51 5.175194567908774E-5 0.0 0.0 0.6852992646824798 0.0 52-53 5.175194567908774E-5 0.0 0.0 0.7948193197258493 0.0 54-55 5.175194567908774E-5 0.0 0.0 0.9144310041766408 0.0 56-57 5.175194567908774E-5 0.0 0.0 1.0372642472459557 0.0 58-59 5.175194567908774E-5 0.0 0.0 1.16805435196343 0.0 60-61 5.175194567908774E-5 0.0 0.0 1.3005263949154748 0.0 62-63 5.175194567908774E-5 0.0 0.0 1.4335806472564094 0.0 64-65 5.175194567908774E-5 0.0 0.0 1.5864558947924348 0.0 66-67 5.175194567908774E-5 0.0 0.0 1.7609505176358986 0.0 68-69 5.175194567908774E-5 0.0 0.0 1.9177459750571146 0.0 70-71 5.175194567908774E-5 0.0 0.0 2.104738692782078 0.0 72-73 5.175194567908774E-5 0.0 0.0 2.333223532955251 0.0 74-75 5.175194567908774E-5 0.0 0.0 2.5344092217827043 0.0 76-77 5.175194567908774E-5 0.0 0.0 2.7262536844150826 0.0 78-79 5.175194567908774E-5 0.0 0.0 2.950456051083311 0.0 80-81 5.175194567908774E-5 0.0 0.0 3.2163057960367842 0.0 82-83 5.175194567908774E-5 0.0 0.0 3.499091365213739 0.0 84-85 5.175194567908774E-5 0.0 0.0 3.7809712753413107 0.0 86-87 5.175194567908774E-5 0.0 0.0 4.119765387729458 0.0 88 5.175194567908774E-5 0.0 0.0 4.402822654621229 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3580 0.0 43.77265 1 GTATCAA 5835 0.0 33.70193 1 TCAACGC 6000 0.0 31.147055 4 ATCAACG 5985 0.0 31.13957 3 ATAACGC 350 0.0 30.720383 3 GTCTCGC 1535 0.0 30.359697 1 GTACCTG 3025 0.0 29.626278 1 TACCTGG 2980 0.0 29.20842 2 TCGCTCT 1550 0.0 29.068748 4 CAACGCA 6440 0.0 29.01857 5 TATCAAC 6995 0.0 27.375322 2 TCTGTCG 1635 0.0 27.111967 8 CTCGCTC 1545 0.0 26.843054 3 CGCTCTG 1525 0.0 26.523222 5 TATAACG 425 0.0 26.50386 2 AACGCAG 7305 0.0 25.932867 6 TCTCGCT 1680 0.0 25.295555 2 GTACATG 7560 0.0 25.063652 1 GTGGTAT 2125 0.0 23.135386 1 CGCAGAG 7955 0.0 23.08546 8 >>END_MODULE