FastQCFastQC Report
Wed 25 May 2016
SRR2049509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864589
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188180.48693405689453656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176560.4568661764549865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114370.2959435013658632No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105160.2721117303806433No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72530.18767843100521167No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.13020789532858476No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89500.060.7090341
GTATCAA160250.039.1654051
GTGGTAT31650.037.3396761
TATAACG3700.036.87812
TGGTATC32700.035.3964462
ATAACGC3950.034.5440443
ATCAACG203550.030.512313
TCAACGC206700.030.1838974
TATCAAC206950.030.0792312
TCTGTCG18300.029.7905678
CAACGCA213900.029.1786235
CGCTCTG17200.028.1440325
AACGCAG223800.027.9928826
TACCTGG29200.026.5872482
GTCTCGC19050.026.445961
GTACCTG30500.026.3977451
GCGGTAT2500.026.3668691
TCGCTCT20050.025.3443814
CGCAGAG250900.024.9128198
ACGCAGA253850.024.6233087
Produced by FastQC (version 0.11.5)