##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049509_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3864589 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.714106985244744 31.0 31.0 34.0 30.0 34.0 2 31.727739741535256 31.0 31.0 34.0 30.0 34.0 3 31.752947337996357 33.0 31.0 34.0 30.0 34.0 4 35.491178492719406 37.0 35.0 37.0 33.0 37.0 5 35.635216060491814 37.0 35.0 37.0 33.0 37.0 6 35.73332196515593 37.0 35.0 37.0 33.0 37.0 7 35.75560971684182 37.0 35.0 37.0 33.0 37.0 8 35.75663078273006 37.0 35.0 37.0 33.0 37.0 9 37.47164394454365 39.0 37.0 39.0 34.0 39.0 10-11 37.445856079391625 39.0 37.0 39.0 34.0 39.0 12-13 37.437042334902884 39.0 37.0 39.0 34.0 39.0 14-15 38.68450797743304 40.0 38.0 41.0 34.0 41.0 16-17 38.694823822145125 40.0 38.0 41.0 34.0 41.0 18-19 38.787090554778274 40.0 38.0 41.0 35.0 41.0 20-21 38.803111533982005 40.0 38.0 41.0 35.0 41.0 22-23 38.735910468098936 40.0 38.0 41.0 34.0 41.0 24-25 38.67401979874186 40.0 38.0 41.0 34.0 41.0 26-27 38.555597115243046 40.0 38.0 41.0 34.0 41.0 28-29 38.43399116956551 40.0 38.0 41.0 34.0 41.0 30-31 38.253575477237035 40.0 38.0 41.0 34.0 41.0 32-33 38.00789488869321 40.0 37.0 41.0 33.5 41.0 34-35 37.83347064849586 40.0 37.0 41.0 33.0 41.0 36-37 37.626978444538345 40.0 37.0 41.0 33.0 41.0 38-39 37.47351801187655 40.0 37.0 41.0 33.0 41.0 40-41 37.31089877862821 40.0 37.0 41.0 32.5 41.0 42-43 37.1292115409944 40.0 37.0 41.0 32.0 41.0 44-45 36.88962293273619 40.0 36.0 41.0 31.0 41.0 46-47 36.55549917468585 40.0 36.0 41.0 30.5 41.0 48-49 36.23458600642914 40.0 36.0 41.0 30.0 41.0 50-51 35.99141784546817 40.0 35.5 41.0 29.0 41.0 52-53 35.857923313449376 39.0 35.0 41.0 28.0 41.0 54-55 35.70073842263692 39.0 35.0 41.0 28.0 41.0 56-57 35.52196805404145 39.0 35.0 41.0 28.0 41.0 58-59 35.34585268963919 39.0 35.0 41.0 28.0 41.0 60-61 35.12849775228362 38.5 35.0 41.0 27.0 41.0 62-63 34.87612214390715 38.0 35.0 40.0 26.5 41.0 64-65 34.55766279415482 37.5 34.0 40.0 26.0 41.0 66-67 34.288776633168496 37.0 34.0 40.0 26.0 41.0 68-69 33.95341613299629 36.5 34.0 39.5 26.0 41.0 70-71 33.634451813634 36.0 34.0 39.0 26.0 41.0 72-73 33.30283867184842 35.5 34.0 39.0 25.0 41.0 74-75 32.98631963191946 35.0 33.0 38.5 25.0 40.0 76-77 32.719285931828715 35.0 33.0 37.0 24.5 39.5 78-79 32.41042216908447 35.0 33.0 37.0 24.0 39.0 80-81 32.12897619902142 35.0 33.0 36.0 23.5 39.0 82-83 31.8674887549491 35.0 33.0 36.0 23.0 38.5 84-85 31.609975730925072 35.0 33.0 36.0 22.0 37.0 86-87 31.34805266485 35.0 33.0 35.0 20.5 37.0 88-89 31.156820427735006 35.0 32.5 35.0 20.0 36.5 90-91 31.018872382030793 35.0 33.0 35.0 20.0 36.0 92-93 30.85579967235843 35.0 32.5 35.0 18.5 36.0 94-95 30.66258960525945 35.0 32.0 35.0 16.5 36.0 96-97 30.481574754779874 35.0 32.0 35.0 6.0 35.5 98-99 30.29978453077417 35.0 32.0 35.0 2.0 35.0 100 30.18019716973784 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3552467010620006 1101 2 0.40039797875760286 1101 3 0.4817697367557514 1101 4 0.3875208048176191 1101 5 0.2952720665292716 1101 6 0.2707069340131838 1101 7 0.28049301006566907 1101 8 0.27081061603274037 1101 9 0.24962851427385147 1101 10-11 0.3226932245441958 1101 12-13 0.2921516978425984 1101 14-15 0.4411742669969385 1101 16-17 0.4580440610668717 1101 18-19 0.43012528810560724 1101 20-21 0.3468377528996669 1101 22-23 0.4092742626654555 1101 24-25 0.4417730811938867 1101 26-27 0.4566023849389964 1101 28-29 0.37277467453640156 1101 30-31 0.37438732237554717 1101 32-33 0.4005092329708049 1101 34-35 0.45145825241989 1101 36-37 0.4591952454829169 1101 38-39 0.5157077548926097 1101 40-41 0.5024808182677916 1101 42-43 0.4456895699509502 1101 44-45 0.4424434251069158 1101 46-47 0.39399420565990084 1101 48-49 0.4549263375062438 1101 50-51 0.5153690768694901 1101 52-53 0.4827404130776145 1101 54-55 0.41011950559012433 1101 56-57 0.4153542786517903 1101 58-59 0.45184321091514335 1101 60-61 0.5169298159862663 1101 62-63 0.47485558208889245 1101 64-65 0.4336880361254529 1101 66-67 0.4938130265485654 1101 68-69 0.4629058276379254 1101 70-71 0.4739673255168029 1101 72-73 0.3914901372457251 1101 74-75 0.4388204352795029 1101 76-77 0.3500369950544666 1101 78-79 0.3599819441696255 1101 80-81 0.3872023128321658 1101 82-83 0.42954685017339145 1101 84-85 0.39492259924023543 1101 86-87 0.4428856848297542 1101 88-89 0.4455602231861313 1101 90-91 0.4189937411818754 1101 92-93 0.506555742426837 1101 94-95 0.4399744233904883 1101 96-97 0.42263774788144914 1101 98-99 0.5427260309823119 1101 100 0.5383953780995157 1102 1 0.19766499675640148 1102 2 0.3105429410903682 1102 3 0.327649727438434 1102 4 0.30961296908898106 1102 5 0.25522635806802185 1102 6 0.20609007565874293 1102 7 0.2775131322414168 1102 8 0.2671672240080696 1102 9 0.35782036114082416 1102 10-11 0.33409793862555404 1102 12-13 0.2759084835421035 1102 14-15 0.35005182700504633 1102 16-17 0.38823666655793687 1102 18-19 0.40586089082906796 1102 20-21 0.390755077332372 1102 22-23 0.36224291831829447 1102 24-25 0.3425084484639882 1102 26-27 0.37844404010050425 1102 28-29 0.3620004614414256 1102 30-31 0.4281469288726285 1102 32-33 0.44399637416641013 1102 34-35 0.4794097498236667 1102 36-37 0.5213594094019598 1102 38-39 0.5536085164084668 1102 40-41 0.5074059885370517 1102 42-43 0.522439190705235 1102 44-45 0.4871477441041705 1102 46-47 0.5482681053034497 1102 48-49 0.6065445553882896 1102 50-51 0.6562000985593244 1102 52-53 0.6170259474333761 1102 54-55 0.582038843779145 1102 56-57 0.6026744884559889 1102 58-59 0.5360412328946893 1102 60-61 0.5471075912319066 1102 62-63 0.5410535097544411 1102 64-65 0.5463168357457633 1102 66-67 0.5511337822066054 1102 68-69 0.5069109662251137 1102 70-71 0.5092533608907885 1102 72-73 0.5489468522959555 1102 74-75 0.5182830425003218 1102 76-77 0.5173418287593989 1102 78-79 0.5527955129952389 1102 80-81 0.5402238990635411 1102 82-83 0.5525919074568684 1102 84-85 0.5512720699941376 1102 86-87 0.5538440755120071 1102 88-89 0.5723055460875344 1102 90-91 0.512442941224112 1102 92-93 0.5510838693535725 1102 94-95 0.6004806408183327 1102 96-97 0.618833007296665 1102 98-99 0.6725936644094226 1102 100 0.6894067729809343 1103 1 0.2421713815507367 1103 2 0.2546636731321854 1103 3 0.30393036886390945 1103 4 0.2506527331004804 1103 5 0.2056798206887862 1103 6 0.23380659739345333 1103 7 0.2097466293004544 1103 8 0.1974090919032676 1103 9 0.22662712333389834 1103 10-11 0.2255657365572219 1103 12-13 0.19309628513094168 1103 14-15 0.3282089224061622 1103 16-17 0.34229741734270647 1103 18-19 0.30435440698661154 1103 20-21 0.30333780791132625 1103 22-23 0.2554970427836878 1103 24-25 0.3043191433754515 1103 26-27 0.30005856258696184 1103 28-29 0.29008873109613376 1103 30-31 0.3348521692801967 1103 32-33 0.31485825790931443 1103 34-35 0.3142052281499019 1103 36-37 0.34259450138973335 1103 38-39 0.27710844529699585 1103 40-41 0.27162158455278274 1103 42-43 0.2034595069989038 1103 44-45 0.23056450431628406 1103 46-47 0.24638190524655101 1103 48-49 0.23138731649437005 1103 50-51 0.2816289261343172 1103 52-53 0.2744740805032535 1103 54-55 0.25754776699907467 1103 56-57 0.26489642355152654 1103 58-59 0.21827104739533354 1103 60-61 0.2321163087365079 1103 62-63 0.23650450252138455 1103 64-65 0.30245181928853526 1103 66-67 0.3265259111825003 1103 68-69 0.310998923497273 1103 70-71 0.30158322669328186 1103 72-73 0.2780826828271614 1103 74-75 0.1820520330729707 1103 76-77 0.17396719574539077 1103 78-79 0.1753145162663614 1103 80-81 0.15543651601651476 1103 82-83 0.24477189948222744 1103 84-85 0.2525277271657309 1103 86-87 0.2325581896154425 1103 88-89 0.22745906432762908 1103 90-91 0.10345168920505543 1103 92-93 0.11431501592447546 1103 94-95 0.12224110055399962 1103 96-97 0.16467933540499757 1103 98-99 0.14425774270315372 1103 100 0.16076021477619307 1104 1 0.23387156201976111 1104 2 0.22986138085469676 1104 3 0.3332081840081784 1104 4 0.30212115525876015 1104 5 0.20699105135919638 1104 6 0.22611514191469695 1104 7 0.20193560779491548 1104 8 0.22675513535750014 1104 9 0.2581785091798281 1104 10-11 0.23437796462018667 1104 12-13 0.25057488281214546 1104 14-15 0.38077099533599323 1104 16-17 0.3639542755591947 1104 18-19 0.3357437581638365 1104 20-21 0.27941688373555706 1104 22-23 0.3079286525298599 1104 24-25 0.33585027867152917 1104 26-27 0.3361617513977606 1104 28-29 0.3945597134808736 1104 30-31 0.3951506787307508 1104 32-33 0.3559415987596637 1104 34-35 0.3176495167381006 1104 36-37 0.32421390365646374 1104 38-39 0.3111409624130985 1104 40-41 0.37108420336962666 1104 42-43 0.3448089200961988 1104 44-45 0.41315260328910597 1104 46-47 0.48172725733672195 1104 48-49 0.5065751259660587 1104 50-51 0.6063180998695046 1104 52-53 0.5388181897121456 1104 54-55 0.5059949826846832 1104 56-57 0.5129638430502936 1104 58-59 0.5135193724115084 1104 60-61 0.48254371944489804 1104 62-63 0.4843872757509473 1104 64-65 0.46212177467678117 1104 66-67 0.46892139079967166 1104 68-69 0.39654744702333034 1104 70-71 0.3726150118836813 1104 72-73 0.41011529922847245 1104 74-75 0.43155827078718545 1104 76-77 0.39741948163592866 1104 78-79 0.37743834187067904 1104 80-81 0.3307027850176709 1104 82-83 0.4085896457443532 1104 84-85 0.47866140985383865 1104 86-87 0.5102634239436661 1104 88-89 0.4065202076694341 1104 90-91 0.40384026365715187 1104 92-93 0.4100094887048904 1104 94-95 0.48147432211075625 1104 96-97 0.5367993227885108 1104 98-99 0.6191542348478158 1104 100 0.6189739164980033 1105 1 0.25782487090581085 1105 2 0.31171809535490524 1105 3 0.38018228761988837 1105 4 0.31992238227402936 1105 5 0.2714532183885012 1105 6 0.24592023224028736 1105 7 0.21960195324578535 1105 8 0.2485280139888033 1105 9 0.31781441616386985 1105 10-11 0.24815047697827453 1105 12-13 0.2574057183656464 1105 14-15 0.3190618511507992 1105 16-17 0.3003322404463802 1105 18-19 0.2768779696808039 1105 20-21 0.3232382179874804 1105 22-23 0.39023909524080835 1105 24-25 0.38468709959444425 1105 26-27 0.40768049165045994 1105 28-29 0.4151407507026619 1105 30-31 0.4793085773690038 1105 32-33 0.4782469107386902 1105 34-35 0.40687279950537913 1105 36-37 0.4157842709907058 1105 38-39 0.5054480447126579 1105 40-41 0.5371407053732256 1105 42-43 0.5349058226166505 1105 44-45 0.5749279964150915 1105 46-47 0.5684764081497207 1105 48-49 0.5179004826827409 1105 50-51 0.6121740382193295 1105 52-53 0.6109563241315072 1105 54-55 0.5869313921316248 1105 56-57 0.49463695583482803 1105 58-59 0.5782263320386107 1105 60-61 0.542228579134644 1105 62-63 0.5568562188347101 1105 64-65 0.5778130644962758 1105 66-67 0.6068915686020588 1105 68-69 0.5939725317966875 1105 70-71 0.5713744067189381 1105 72-73 0.5755373927705847 1105 74-75 0.5947528999428258 1105 76-77 0.602163083119045 1105 78-79 0.5464206837227792 1105 80-81 0.5623295031951443 1105 82-83 0.582564088013438 1105 84-85 0.6045574929106543 1105 86-87 0.6187989851483557 1105 88-89 0.6135514760648526 1105 90-91 0.5883020818548275 1105 92-93 0.5552774713388899 1105 94-95 0.635501539698847 1105 96-97 0.6699292000724526 1105 98-99 0.6910335652654958 1105 100 0.7502544047460127 1106 1 0.19365776693939551 1106 2 0.15661892430457058 1106 3 0.24785495702554527 1106 4 0.22263398202316864 1106 5 0.19576264742435256 1106 6 0.18089670338063968 1106 7 0.19418813969333115 1106 8 0.22154452898711696 1106 9 0.18719583967054376 1106 10-11 0.19148679674891866 1106 12-13 0.17991953063819466 1106 14-15 0.218108153728096 1106 16-17 0.2366329405526102 1106 18-19 0.2369174333166626 1106 20-21 0.22564131876266913 1106 22-23 0.24174225446484598 1106 24-25 0.2580269177630754 1106 26-27 0.2712329868188377 1106 28-29 0.2964725737519842 1106 30-31 0.2805141167835785 1106 32-33 0.28370419828567606 1106 34-35 0.32275531306167693 1106 36-37 0.2574755331017755 1106 38-39 0.27838780827214293 1106 40-41 0.28230971639317914 1106 42-43 0.29770108527380756 1106 44-45 0.24737875219704364 1106 46-47 0.27168507905712147 1106 48-49 0.32186920188510726 1106 50-51 0.3385124954258174 1106 52-53 0.33268739118555857 1106 54-55 0.34214242659084704 1106 56-57 0.366964841053246 1106 58-59 0.2783771471226757 1106 60-61 0.37885906724648777 1106 62-63 0.3723473384500551 1106 64-65 0.35600742183630274 1106 66-67 0.34281798278678366 1106 68-69 0.34660831870158404 1106 70-71 0.3901554709998649 1106 72-73 0.3465337109200135 1106 74-75 0.40166970206575314 1106 76-77 0.3493225546049672 1106 78-79 0.34114373432581857 1106 80-81 0.24606476832678226 1106 82-83 0.31840180501010806 1106 84-85 0.41043343630486717 1106 86-87 0.4286767118722814 1106 88-89 0.41436775470798537 1106 90-91 0.4005730114474346 1106 92-93 0.4168087030736274 1106 94-95 0.4042640027416837 1106 96-97 0.4291299441219749 1106 98-99 0.4503064104443233 1106 100 0.52083432820789 1107 1 0.17389761433206274 1107 2 0.13548561365635337 1107 3 0.2408248587425348 1107 4 0.22652565652445844 1107 5 0.18470984661880152 1107 6 0.15784511683689573 1107 7 0.16969398959096083 1107 8 0.16103477335535388 1107 9 0.15668374578697097 1107 10-11 0.1506573411141332 1107 12-13 0.13768395771042918 1107 14-15 0.198673911355435 1107 16-17 0.13646991290539745 1107 18-19 0.23300010834368123 1107 20-21 0.2829810861660391 1107 22-23 0.29758686019714986 1107 24-25 0.30700908082397405 1107 26-27 0.23984916744323925 1107 28-29 0.23918519524023907 1107 30-31 0.2170732194037086 1107 32-33 0.2407161696774338 1107 34-35 0.25188986642572786 1107 36-37 0.2884530735543436 1107 38-39 0.3380263265937842 1107 40-41 0.32445733289109313 1107 42-43 0.3139534835719928 1107 44-45 0.30877344212817803 1107 46-47 0.3059457264577361 1107 48-49 0.23135932964760997 1107 50-51 0.2856224920687964 1107 52-53 0.24648851380306525 1107 54-55 0.21568094249667524 1107 56-57 0.247265582860976 1107 58-59 0.2640328147443398 1107 60-61 0.25668123982107716 1107 62-63 0.22231666946845507 1107 64-65 0.16283240840292734 1107 66-67 0.20757452480080474 1107 68-69 0.20966029690668364 1107 70-71 0.23725998326793984 1107 72-73 0.2965399791755772 1107 74-75 0.17284591692872198 1107 76-77 0.20350280318942282 1107 78-79 0.145490807217854 1107 80-81 0.09359855985928078 1107 82-83 0.12484879930763881 1107 84-85 0.11930616181659204 1107 86-87 0.23095800819551116 1107 88-89 0.1903338429711603 1107 90-91 0.1622655676554423 1107 92-93 0.12002676187605843 1107 94-95 0.2568671866807577 1107 96-97 0.26104319816005983 1107 98-99 0.34503506137122386 1107 100 0.24868304671925046 1108 1 0.04823814124806347 1108 2 0.06999051730410599 1108 3 0.16631141934037075 1108 4 0.08877424355292618 1108 5 0.05896004207762218 1108 6 0.11925815953566143 1108 7 0.049281182950096536 1108 8 0.07269672414083317 1108 9 0.06061859542771941 1108 10-11 0.04892395947052108 1108 12-13 -0.0019476918234317964 1108 14-15 0.07085786268122973 1108 16-17 0.07271710685934352 1108 18-19 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-0.5518239194614409 2215 30-31 -0.5515642418501372 2215 32-33 -0.6228199961087384 2215 34-35 -0.6962980933938852 2215 36-37 -0.7573252445472889 2215 38-39 -0.8076797963053579 2215 40-41 -0.7987407794585337 2215 42-43 -0.7672300445717113 2215 44-45 -0.9136949347389418 2215 46-47 -0.7969468032152065 2215 48-49 -0.8138377020939842 2215 50-51 -0.8182555513224088 2215 52-53 -0.9810791623157655 2215 54-55 -0.7759490744013036 2215 56-57 -0.7736843977033203 2215 58-59 -0.7409853993878386 2215 60-61 -0.9859624685434483 2215 62-63 -0.9279889211115275 2215 64-65 -0.7669270873452732 2215 66-67 -0.6263885428584501 2215 68-69 -0.623210906908163 2215 70-71 -0.725861889948483 2215 72-73 -0.5843383318819306 2215 74-75 -0.717305135942631 2215 76-77 -0.6593071402503625 2215 78-79 -0.6324800344150958 2215 80-81 -0.6814301704813417 2215 82-83 -0.6974151171156144 2215 84-85 -0.6357690515471539 2215 86-87 -0.6502070135220706 2215 88-89 -0.6088439760968711 2215 90-91 -0.5347720759041614 2215 92-93 -0.7454848036459829 2215 94-95 -0.8161539724690741 2215 96-97 -0.9606008027023663 2215 98-99 -0.8561585228059805 2215 100 -0.8512843637167471 2216 1 -0.6197623622315227 2216 2 -1.0067882791956357 2216 3 -0.7619108120040181 2216 4 -1.1264275911868609 2216 5 -0.6483843663662725 2216 6 -0.8719768786105959 2216 7 -0.638057193661119 2216 8 -0.6106202973180999 2216 9 -0.6142660231668913 2216 10-11 -0.8649987554527243 2216 12-13 -0.6693961841893383 2216 14-15 -1.0078826397629115 2216 16-17 -0.8804983408737996 2216 18-19 -0.8861478610347646 2216 20-21 -0.7439570830404207 2216 22-23 -0.7540344249196238 2216 24-25 -0.8410649693205059 2216 26-27 -0.9300965013130522 2216 28-29 -1.1655430703514256 2216 30-31 -1.2631698247491627 2216 32-33 -0.9872623614286979 2216 34-35 -1.043821451317129 2216 36-37 -1.0080464487046257 2216 38-39 -1.098431333466614 2216 40-41 -0.9740009721879019 2216 42-43 -1.5514300654936193 2216 44-45 -1.238529915855075 2216 46-47 -0.9615917261196074 2216 48-49 -0.9271951923711086 2216 50-51 -1.0951309302745074 2216 52-53 -0.9517430651590857 2216 54-55 -1.0802946405703437 2216 56-57 -1.232139047849671 2216 58-59 -1.0895413464188621 2216 60-61 -1.2568445965336394 2216 62-63 -1.2301969895066236 2216 64-65 -1.1916565869429192 2216 66-67 -1.0916106753471269 2216 68-69 -1.3941401757918541 2216 70-71 -1.1631671275103272 2216 72-73 -0.9518316000442368 2216 74-75 -0.9987581318607432 2216 76-77 -1.126949188691043 2216 78-79 -1.1506322561294695 2216 80-81 -0.9704445054088993 2216 82-83 -1.0170525508749328 2216 84-85 -1.033115119756662 2216 86-87 -1.283529765282772 2216 88-89 -1.0841437309918653 2216 90-91 -0.9641226017927416 2216 92-93 -1.0491065837137619 2216 94-95 -1.2252218525162064 2216 96-97 -1.2919400209580907 2216 98-99 -1.3544766156341872 2216 100 -1.395432978951746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 233.0 3 25.0 4 40.0 5 157.0 6 492.0 7 1156.0 8 2545.0 9 4246.0 10 6181.0 11 9033.0 12 13496.0 13 19200.0 14 19735.0 15 21902.0 16 26698.0 17 36555.0 18 36153.0 19 19465.0 20 14077.0 21 13022.0 22 13389.0 23 14048.0 24 16440.0 25 19587.0 26 24027.0 27 30369.0 28 39003.0 29 50644.0 30 64018.0 31 82139.0 32 105347.0 33 136389.0 34 186491.0 35 272086.0 36 427444.0 37 798156.0 38 980789.0 39 358454.0 40 1358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.995162250380794 19.520196730836002 12.685597021282213 22.79904399750099 2 17.990796946737127 21.194968569733323 37.32799908502513 23.48623539850442 3 18.412773001216948 25.14282890108107 30.592024145387775 25.852373952314206 4 13.282269866604768 18.131140329629307 37.1226878112182 31.463901992547733 5 11.618522896409814 35.36794585430179 33.80796556171297 19.20556568757543 6 28.566190464730962 37.54307437087548 18.348640471031846 15.542094693361713 7 25.092034366397048 32.22580719450374 23.111642661095395 19.57051577800382 8 24.597466897514845 32.53246334862517 22.589931296704513 20.28013845715547 9 23.568682389264982 16.625610091223226 23.969191007781944 35.83651651172985 10-11 22.766832385022056 25.89342618322414 29.031120773774393 22.308620657979414 12-13 24.913690670260323 23.736579423214067 29.142925455443113 22.206804451082498 14-15 22.36986193612359 25.956026877897465 28.00596079064573 23.668150395333214 16-17 20.070129061589732 28.717801556646773 28.10420461270267 23.107864769060825 18-19 19.45188738052093 25.813340047285756 32.63722222466606 22.097550347527253 20-21 21.523233130353578 26.060520277835497 31.77737658519444 20.638870006616486 22-23 20.765507017693213 26.63434585152522 31.886172112998302 20.713975017783262 24-25 20.396062815476625 26.00945404543666 32.45289473214357 21.141588406943146 26-27 20.25123761559607 25.428839318856326 32.79180952900936 21.52811353653824 28-29 20.648162990914802 25.651975174038803 32.448309112741384 21.251552722305004 30-31 21.281977137216167 26.967449870005193 31.40607220323567 20.34450078954297 32-33 19.941688472573073 26.201515685697373 32.68011139610631 21.176684445623238 34-35 19.920591814550008 25.11574969550449 32.80893518042928 22.154723309516225 36-37 21.691905659308144 26.57307154784118 31.07606009332428 20.65896269952639 38-39 21.31207225399648 26.492532582378097 31.468016391911274 20.727378771714147 40-41 20.434126371523593 26.147605864426982 31.831379740510567 21.586888023538854 42-43 20.370717300080294 26.362298811076673 31.379675820637072 21.88730806820596 44-45 21.295149367759418 26.798528899192124 30.83510303424245 21.071218698806003 46-47 20.93024639877617 28.04811844157296 29.786854436526113 21.23478072312476 48-49 20.861248117199526 26.89630126256634 30.55031207717043 21.6921385430637 50-51 21.117329682406073 26.374719278039656 30.301682792141676 22.2062682474126 52-53 21.059315751299813 26.799486310187188 29.903813833760847 22.23738410475215 54-55 21.512015896127636 27.999976194104985 29.337285284411873 21.150722625355503 56-57 20.456185638369305 27.48403517165732 29.461412326123167 22.598366863850206 58-59 20.2014706633899 26.529230518432456 30.436806563192793 22.832492254984853 60-61 21.500009444730086 27.50793163257464 29.21630217340059 21.775756749294686 62-63 21.18499535138148 27.237734724184126 29.46233092315897 22.114939001275427 64-65 20.96990132715277 26.70260666787594 30.131004875292046 22.196487129679248 66-67 20.826548437621696 26.956294705594825 29.395946114839123 22.821210741944356 68-69 22.01357272578956 27.426930448092985 29.005520251379934 21.55397657473752 70-71 22.110475395960606 26.740683161909324 29.306259992977264 21.842581449152807 72-73 21.307272261034743 27.064533899982635 30.060169399643794 21.568024439338828 74-75 21.19618670963458 26.90675515559352 29.75276284231001 22.144295292461887 76-77 21.432822481252213 27.366338309196657 28.930320921577945 22.270518287973186 78-79 21.77214036916292 27.703197319450673 29.025992727284017 21.49866958410239 80-81 21.252105987278817 27.022077707340287 29.480639148864157 22.24517715651674 82-83 20.929328099815322 27.16658252636705 29.610614564026022 22.293474809791604 84-85 21.27104589905938 27.20440647116679 29.23301805185493 22.291529577918894 86-87 21.68033909944887 27.340979855813902 29.069546593441114 21.909134451296115 88-89 20.940579039806963 27.245469277122076 29.281973819974315 22.531977863096646 90-91 20.568342749173457 27.00641750334512 29.222488716129302 23.202751031352122 92-93 21.19704955417403 27.49632979723351 28.686178029966346 22.620442618626114 94-95 21.08595179855575 27.089797501560973 29.11755063237258 22.706700067510692 96-97 20.7455075747392 27.057990779613405 29.394138599750015 22.802363045897383 98-99 20.750928591750455 27.018128838296757 29.310753266486202 22.920189303466593 100 21.15032102006026 27.379134886387984 28.804990793250244 22.665553300301507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1851.0 1 1539.5 2 4849.5 3 7748.0 4 4353.5 5 1656.0 6 2693.0 7 5802.0 8 8745.0 9 9452.5 10 7264.0 11 4596.0 12 4109.5 13 4623.0 14 5195.0 15 5702.5 16 6221.5 17 6931.0 18 7851.0 19 8812.5 20 9881.5 21 11430.5 22 14230.0 23 18479.5 24 22940.5 25 27466.0 26 34390.5 27 43248.5 28 53462.0 29 63397.0 30 70424.0 31 77352.5 32 84525.5 33 90776.0 34 97452.0 35 102918.0 36 106062.5 37 109176.5 38 111321.5 39 111630.0 40 112165.0 41 110946.0 42 107882.0 43 106933.5 44 106613.5 45 107505.5 46 110511.5 47 114001.0 48 118551.5 49 124587.0 50 127618.5 51 123931.5 52 116421.0 53 110634.5 54 108298.5 55 105520.0 56 101271.5 57 95365.0 58 88551.0 59 81318.0 60 71288.5 61 58962.0 62 47203.5 63 36965.0 64 28133.0 65 20910.5 66 15429.5 67 11085.0 68 7771.5 69 5489.5 70 3798.0 71 2645.0 72 1919.5 73 1386.0 74 1032.5 75 703.5 76 479.0 77 369.5 78 268.0 79 219.5 80 145.5 81 55.5 82 31.5 83 15.5 84 8.0 85 5.0 86 2.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.058376194726010966 2 5.175194567908774E-5 3 0.0 4 0.11789093225696187 5 0.0013714265604958251 6 0.001345550587656281 7 0.0 8 0.0 9 7.76279185186316E-5 10-11 0.0 12-13 2.587597283954387E-5 14-15 2.587597283954387E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.2937986419771935E-5 28-29 1.4231785061749128E-4 30-31 2.975736876547545E-4 32-33 3.88139592593158E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.6819382345703514E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.2937986419771935E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.0700778271635095E-4 80-81 3.105116740745264E-4 82-83 3.105116740745264E-4 84-85 0.0 86-87 0.0 88-89 3.88139592593158E-5 90-91 5.175194567908774E-5 92-93 5.304574432106493E-4 94-95 4.140155654327019E-4 96-97 1.2937986419771935E-5 98-99 1.2937986419771935E-5 100 8.539071037049477E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3864589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.06733637072693 #Duplication Level Percentage of deduplicated Percentage of total 1 82.04090545983921 55.022650026340436 2 11.778105107590857 15.79852274121146 3 2.8750123121414304 5.78458253425112 4 1.150365593277635 3.08607824774648 5 0.561398838588306 1.8825762372868673 6 0.3529124629570394 1.4201339317536883 7 0.24158221181212036 1.1341592822551376 8 0.1697540768983117 0.9107963020513054 9 0.12472855159820603 0.752869055256341 >10 0.6513800519725736 7.71992009026265 >50 0.03382247696392765 1.5509819182594375 >100 0.018219287859608416 2.144827704523286 >500 0.0011961835171792235 0.5331328856996895 >1k 3.8586561473549785E-4 0.42881468165490166 >5k 7.717312294709828E-5 0.31792325673689864 >10k+ 1.5434624589419655E-4 1.5120311047103605 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18818 0.48693405689453656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17656 0.4568661764549865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11437 0.2959435013658632 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10516 0.2721117303806433 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7253 0.18767843100521167 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5032 0.13020789532858476 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.587597283954387E-5 0.0 2 0.0 0.0 0.0 2.587597283954387E-5 0.0 3 0.0 0.0 0.0 2.587597283954387E-5 0.0 4 0.0 0.0 0.0 5.175194567908774E-5 0.0 5 0.0 0.0 0.0 5.175194567908774E-5 0.0 6 0.0 0.0 0.0 5.175194567908774E-5 0.0 7 0.0 0.0 0.0 5.175194567908774E-5 0.0 8 0.0 0.0 0.0 5.175194567908774E-5 0.0 9 2.587597283954387E-5 0.0 0.0 5.175194567908774E-5 0.0 10-11 2.587597283954387E-5 0.0 0.0 7.76279185186316E-5 0.0 12-13 2.587597283954387E-5 5.175194567908774E-5 0.0 7.76279185186316E-5 0.0 14-15 2.587597283954387E-5 5.175194567908774E-5 0.0 1.2937986419771935E-4 0.0 16-17 2.587597283954387E-5 5.175194567908774E-5 0.0 1.2937986419771935E-4 2.587597283954387E-5 18-19 2.587597283954387E-5 5.175194567908774E-5 0.0 1.2937986419771935E-4 2.587597283954387E-5 20-21 2.587597283954387E-5 5.175194567908774E-5 0.0 1.6819382345703514E-4 2.587597283954387E-5 22-23 2.587597283954387E-5 5.175194567908774E-5 0.0 4.3989153827224577E-4 2.587597283954387E-5 24-25 2.587597283954387E-5 5.175194567908774E-5 0.0 0.0010867908592608424 2.587597283954387E-5 26-27 2.587597283954387E-5 5.175194567908774E-5 0.0 0.0018889460172867023 2.587597283954387E-5 28-29 2.587597283954387E-5 5.175194567908774E-5 0.0 0.004670613097537669 2.587597283954387E-5 30-31 2.587597283954387E-5 5.175194567908774E-5 0.0 0.013144994202488285 2.587597283954387E-5 32-33 2.587597283954387E-5 5.175194567908774E-5 0.0 0.030016128493870888 2.587597283954387E-5 34-35 2.587597283954387E-5 5.175194567908774E-5 0.0 0.05758697755440488 2.587597283954387E-5 36-37 2.587597283954387E-5 5.175194567908774E-5 0.0 0.09484837844334805 2.587597283954387E-5 38-39 2.587597283954387E-5 5.175194567908774E-5 0.0 0.1494078671755263 2.587597283954387E-5 40-41 2.587597283954387E-5 5.175194567908774E-5 0.0 0.22121369180526052 2.587597283954387E-5 42-43 2.587597283954387E-5 5.175194567908774E-5 0.0 0.29856991260907695 2.587597283954387E-5 44-45 2.587597283954387E-5 5.175194567908774E-5 0.0 0.38255038245981654 2.587597283954387E-5 46-47 2.587597283954387E-5 5.175194567908774E-5 0.0 0.47443596201303684 2.587597283954387E-5 48-49 2.587597283954387E-5 5.175194567908774E-5 0.0 0.5779527913576321 2.587597283954387E-5 50-51 2.587597283954387E-5 5.175194567908774E-5 0.0 0.6915094981639703 2.587597283954387E-5 52-53 2.587597283954387E-5 5.175194567908774E-5 0.0 0.802607987550552 2.587597283954387E-5 54-55 2.587597283954387E-5 5.175194567908774E-5 0.0 0.9278994480396233 2.587597283954387E-5 56-57 2.587597283954387E-5 5.175194567908774E-5 0.0 1.0689752519608164 2.587597283954387E-5 58-59 2.587597283954387E-5 5.175194567908774E-5 0.0 1.2202203132079505 2.587597283954387E-5 60-61 2.587597283954387E-5 5.175194567908774E-5 0.0 1.3742858554945947 2.587597283954387E-5 62-63 2.587597283954387E-5 5.175194567908774E-5 0.0 1.528338459794819 3.88139592593158E-5 64-65 2.587597283954387E-5 5.175194567908774E-5 0.0 1.706235773066683 5.175194567908774E-5 66-67 2.587597283954387E-5 5.175194567908774E-5 0.0 1.911936819154637 5.175194567908774E-5 68-69 2.587597283954387E-5 5.175194567908774E-5 0.0 2.1188799119388895 5.175194567908774E-5 70-71 2.587597283954387E-5 5.175194567908774E-5 0.0 2.3407534410515582 5.175194567908774E-5 72-73 2.587597283954387E-5 5.175194567908774E-5 0.0 2.6045201701914484 5.175194567908774E-5 74-75 2.587597283954387E-5 5.175194567908774E-5 0.0 2.862658875238738 5.175194567908774E-5 76-77 2.587597283954387E-5 5.175194567908774E-5 0.0 3.1188956962823213 5.175194567908774E-5 78-79 2.587597283954387E-5 5.175194567908774E-5 0.0 3.4075939252531127 5.175194567908774E-5 80-81 2.587597283954387E-5 5.175194567908774E-5 0.0 3.7140301336054105 5.175194567908774E-5 82-83 2.587597283954387E-5 5.175194567908774E-5 0.0 4.0398345076281075 5.175194567908774E-5 84-85 2.587597283954387E-5 5.175194567908774E-5 0.0 4.365962331311298 5.175194567908774E-5 86-87 2.587597283954387E-5 5.175194567908774E-5 0.0 4.719065856679714 5.175194567908774E-5 88 2.587597283954387E-5 5.175194567908774E-5 0.0 5.006871364587541 5.175194567908774E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8950 0.0 60.709034 1 GTATCAA 16025 0.0 39.165405 1 GTGGTAT 3165 0.0 37.339676 1 TATAACG 370 0.0 36.8781 2 TGGTATC 3270 0.0 35.396446 2 ATAACGC 395 0.0 34.544044 3 ATCAACG 20355 0.0 30.51231 3 TCAACGC 20670 0.0 30.183897 4 TATCAAC 20695 0.0 30.079231 2 TCTGTCG 1830 0.0 29.790567 8 CAACGCA 21390 0.0 29.178623 5 CGCTCTG 1720 0.0 28.144032 5 AACGCAG 22380 0.0 27.992882 6 TACCTGG 2920 0.0 26.587248 2 GTCTCGC 1905 0.0 26.44596 1 GTACCTG 3050 0.0 26.397745 1 GCGGTAT 250 0.0 26.366869 1 TCGCTCT 2005 0.0 25.344381 4 CGCAGAG 25090 0.0 24.912819 8 ACGCAGA 25385 0.0 24.623308 7 >>END_MODULE