FastQCFastQC Report
Wed 25 May 2016
SRR2049508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5060331
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.045.516971
GTATCAA78850.034.319181
TCAACGC82200.031.6691724
ATCAACG83150.031.3073483
CAACGCA91350.028.441075
TATCAAC94850.027.7690262
AACGCAG98050.026.6535076
GTACATG130000.024.986951
TACATGG131500.023.9966242
GTACCTG35200.023.978621
ACGCAGA116100.022.4656527
ACATGGG134750.022.4310443
TCTGTCG15950.022.4151028
CGCAGAG116900.022.2214168
TACCTGG36450.022.1692162
GTGGTAT30950.020.329531
GTCTCGC14400.019.5381361
TGGTATC30750.019.4594922
CATGGGG92800.019.0686044
GCAGAGT136450.018.5069569
Produced by FastQC (version 0.11.5)