FastQCFastQC Report
Wed 25 May 2016
SRR2049508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5060331
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165180.3264213348889628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154650.30561241942473727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93400.18457290639683452No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58800.11619793250678662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96650.054.593041
GTATCAA176800.042.569621
ATCAACG220250.033.7058683
TCAACGC223650.033.2144934
TATCAAC227150.032.7027172
GTGGTAT41600.032.2466851
CAACGCA231650.032.137195
AACGCAG241000.031.0067716
TGGTATC44200.029.3765812
ACGCAGA272600.027.3087317
CGCAGAG273600.027.0543258
TACCTGG36200.026.1217292
GTACCTG39000.025.1033861
GCAGAGT295200.024.7404089
ATAACGC5400.023.5225523
TATAACG5700.023.1098752
TCTGTCG17500.023.0957788
GTACATG125900.022.618471
AGAGTAC278200.021.38694810-11
GAGTACT225650.021.36912-13
Produced by FastQC (version 0.11.5)