##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049508_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5060331 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.786995356627855 31.0 31.0 34.0 30.0 34.0 2 31.965879109489084 33.0 31.0 34.0 30.0 34.0 3 31.990547258667466 33.0 31.0 34.0 30.0 34.0 4 35.60593111399235 37.0 35.0 37.0 33.0 37.0 5 35.72815335597612 37.0 35.0 37.0 33.0 37.0 6 35.79586631783573 37.0 35.0 37.0 35.0 37.0 7 35.77911029930651 37.0 35.0 37.0 33.0 37.0 8 35.77457857993874 37.0 35.0 37.0 33.0 37.0 9 37.51751812282635 39.0 37.0 39.0 35.0 39.0 10-11 37.47299326862215 39.0 37.0 39.0 34.5 39.0 12-13 37.45184988887091 39.0 37.0 39.0 34.0 39.0 14-15 38.71510984162894 40.0 38.0 41.0 34.0 41.0 16-17 38.723859960939315 40.0 38.0 41.0 34.0 41.0 18-19 38.799693834257084 40.0 38.0 41.0 35.0 41.0 20-21 38.824080578918654 40.0 38.0 41.0 35.0 41.0 22-23 38.782157827225134 40.0 38.0 41.0 35.0 41.0 24-25 38.73480628045873 40.0 38.0 41.0 34.0 41.0 26-27 38.63604357106284 40.0 38.0 41.0 34.0 41.0 28-29 38.53845608123263 40.0 38.0 41.0 34.0 41.0 30-31 38.40489663620818 40.0 38.0 41.0 34.0 41.0 32-33 38.233884996850996 40.0 38.0 41.0 34.0 41.0 34-35 38.110311262247464 40.0 38.0 41.0 34.0 41.0 36-37 37.953558176332734 40.0 37.5 41.0 33.0 41.0 38-39 37.840868314740675 40.0 37.0 41.0 33.0 41.0 40-41 37.710862787434266 40.0 37.0 41.0 33.0 41.0 42-43 37.56375452514865 40.0 37.0 41.0 33.0 41.0 44-45 37.366834699153074 40.0 37.0 41.0 32.5 41.0 46-47 37.10432291879721 40.0 37.0 41.0 31.5 41.0 48-49 36.85929369047203 40.0 37.0 41.0 31.0 41.0 50-51 36.6451144203808 40.0 36.0 41.0 30.0 41.0 52-53 36.534169108700596 40.0 36.0 41.0 30.0 41.0 54-55 36.394031339056674 39.5 35.5 41.0 30.0 41.0 56-57 36.22558050056409 39.0 35.0 41.0 30.0 41.0 58-59 36.05314395441721 39.0 35.0 41.0 30.0 41.0 60-61 35.85450941845504 39.0 35.0 41.0 29.0 41.0 62-63 35.63930225117685 38.5 35.0 40.5 29.0 41.0 64-65 35.348656738067135 38.0 35.0 40.0 28.5 41.0 66-67 35.091659616732585 37.5 35.0 40.0 28.0 41.0 68-69 34.775904975386 37.0 34.0 40.0 28.0 41.0 70-71 34.4741786653877 36.5 34.0 39.0 28.0 41.0 72-73 34.149853438441085 36.0 34.0 39.0 28.0 41.0 74-75 33.82187677051165 35.5 34.0 39.0 27.0 40.5 76-77 33.533287150583625 35.0 34.0 37.5 27.0 40.0 78-79 33.21544766537999 35.0 34.0 37.0 26.5 39.0 80-81 32.9116686240485 35.0 33.5 37.0 26.0 39.0 82-83 32.622742464870385 35.0 33.0 36.0 26.0 39.0 84-85 32.35337658742086 35.0 33.0 36.0 26.0 37.0 86-87 32.074348397367686 35.0 33.0 35.5 25.5 37.0 88-89 31.874438747188673 35.0 33.0 35.0 25.0 36.5 90-91 31.730182966292126 35.0 33.0 35.0 25.0 36.0 92-93 31.57099496060633 35.0 33.0 35.0 25.0 36.0 94-95 31.384337506775744 35.0 33.0 35.0 24.5 36.0 96-97 31.214091924026313 35.0 33.0 35.0 24.0 36.0 98-99 31.03183566450495 35.0 33.0 35.0 23.0 35.0 100 30.917450656883908 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3639725728536831 1101 2 0.35756106553341027 1101 3 0.44391766497947316 1101 4 0.365918270606727 1101 5 0.26783448687021405 1101 6 0.2739753082252676 1101 7 0.26197413902900735 1101 8 0.2931099371609278 1101 9 0.29221285029997546 1101 10-11 0.3153538830602969 1101 12-13 0.29536338798264694 1101 14-15 0.43640160604312683 1101 16-17 0.4430605467721733 1101 18-19 0.45774507278287757 1101 20-21 0.4166395379364687 1101 22-23 0.4422289059395865 1101 24-25 0.5181098363485575 1101 26-27 0.5443270022255291 1101 28-29 0.4976742905439835 1101 30-31 0.4872731388307301 1101 32-33 0.521723580924224 1101 34-35 0.5154770003128917 1101 36-37 0.4881626870540856 1101 38-39 0.45888625187070886 1101 40-41 0.4951210123238994 1101 42-43 0.5087711162521202 1101 44-45 0.49368281172685613 1101 46-47 0.5589941713168116 1101 48-49 0.5508512966691086 1101 50-51 0.5369214465225411 1101 52-53 0.491753054619565 1101 54-55 0.4385725608244897 1101 56-57 0.43390950119779603 1101 58-59 0.456279779191199 1101 60-61 0.49825702351596135 1101 62-63 0.4885400473783932 1101 64-65 0.4335142353530159 1101 66-67 0.5133239270753833 1101 68-69 0.4554386975521254 1101 70-71 0.4050800073343339 1101 72-73 0.4312148316115483 1101 74-75 0.4315442042079596 1101 76-77 0.33731511137288095 1101 78-79 0.31117926851558764 1101 80-81 0.330233837647846 1101 82-83 0.367022669655249 1101 84-85 0.4202663180218167 1101 86-87 0.47154195510247376 1101 88-89 0.4353202536791798 1101 90-91 0.44224886252376194 1101 92-93 0.46493801385962286 1101 94-95 0.4270249591719377 1101 96-97 0.45940252234435164 1101 98-99 0.5001516122308374 1101 100 0.5043584245804205 1102 1 0.2807423620431919 1102 2 0.2943457983479192 1102 3 0.38395424486598984 1102 4 0.34624363241960765 1102 5 0.26002268042748966 1102 6 0.26293470514752215 1102 7 0.25751201051873096 1102 8 0.2665122693222628 1102 9 0.3260064373747156 1102 10-11 0.3114474681014414 1102 12-13 0.30646591129796263 1102 14-15 0.40499583064489286 1102 16-17 0.3956463541375328 1102 18-19 0.38487734611354085 1102 20-21 0.4114360464093565 1102 22-23 0.3960521724543895 1102 24-25 0.33292277026023953 1102 26-27 0.3238503512279678 1102 28-29 0.3452139612137586 1102 30-31 0.39326253233679864 1102 32-33 0.3977625582341773 1102 34-35 0.485955420142254 1102 36-37 0.502512051313694 1102 38-39 0.44798456327490754 1102 40-41 0.42499784511732486 1102 42-43 0.4223108183381328 1102 44-45 0.4747219917293606 1102 46-47 0.4031716264853742 1102 48-49 0.38857813377224915 1102 50-51 0.37501536628450083 1102 52-53 0.35439701360954246 1102 54-55 0.3275150548345991 1102 56-57 0.34773546479171813 1102 58-59 0.3552249168817383 1102 60-61 0.3264438498886335 1102 62-63 0.3679692188811572 1102 64-65 0.3060088631104918 1102 66-67 0.3599443280737731 1102 68-69 0.3112570795102414 1102 70-71 0.3065995517686986 1102 72-73 0.3908908287954773 1102 74-75 0.40207610060612353 1102 76-77 0.37465767588707166 1102 78-79 0.3165935291445692 1102 80-81 0.32659255833791434 1102 82-83 0.32761832587604545 1102 84-85 0.28740278715498846 1102 86-87 0.34963031873014927 1102 88-89 0.3411295044603264 1102 90-91 0.3106618497273459 1102 92-93 0.3427389478829035 1102 94-95 0.33710657284848367 1102 96-97 0.34486972911509284 1102 98-99 0.4035555076389876 1102 100 0.4552236592216339 1103 1 0.3038633717042316 1103 2 0.33993073086706715 1103 3 0.40071720882194484 1103 4 0.3150091750415882 1103 5 0.22863773575556223 1103 6 0.26952504225816654 1103 7 0.24844731665103126 1103 8 0.2540070469588116 1103 9 0.35222621565988277 1103 10-11 0.29952974702937496 1103 12-13 0.2937710511651659 1103 14-15 0.38285097142290425 1103 16-17 0.4154245628724951 1103 18-19 0.37144244857717723 1103 20-21 0.37309181810450553 1103 22-23 0.363487564934303 1103 24-25 0.38488414538629456 1103 26-27 0.42317194397631397 1103 28-29 0.44610361118260755 1103 30-31 0.47487636928678967 1103 32-33 0.470005588759534 1103 34-35 0.4496936085987997 1103 36-37 0.5023331565706783 1103 38-39 0.48207703720745343 1103 40-41 0.5286957986047724 1103 42-43 0.49500329377386265 1103 44-45 0.6002559424314384 1103 46-47 0.5859147614473841 1103 48-49 0.5526689864844698 1103 50-51 0.6217410574591824 1103 52-53 0.5816149931384302 1103 54-55 0.5944553211877732 1103 56-57 0.6195821201075802 1103 58-59 0.587013066865083 1103 60-61 0.546250224036136 1103 62-63 0.5904978037175894 1103 64-65 0.5897104586112647 1103 66-67 0.6201023690315424 1103 68-69 0.6131199415093533 1103 70-71 0.6258721461259071 1103 72-73 0.5711923774770398 1103 74-75 0.6319154528721214 1103 76-77 0.6337868916066611 1103 78-79 0.5635954817002187 1103 80-81 0.5648832434844024 1103 82-83 0.5453811471153571 1103 84-85 0.622987616065501 1103 86-87 0.6432239485146738 1103 88-89 0.6362241006289011 1103 90-91 0.6119838767827019 1103 92-93 0.645305711651524 1103 94-95 0.6845191128306993 1103 96-97 0.714311768173765 1103 98-99 0.7498580445940419 1103 100 0.7584699937159058 1104 1 0.28195637733509216 1104 2 0.2910788669805342 1104 3 0.35220670901978934 1104 4 0.2865062092403292 1104 5 0.23477915823983153 1104 6 0.2907788906350177 1104 7 0.2105316072710508 1104 8 0.24106430597559125 1104 9 0.30833895258211186 1104 10-11 0.2917865360982148 1104 12-13 0.2635204903338604 1104 14-15 0.3834626820367575 1104 16-17 0.3940609554718577 1104 18-19 0.35956439147271624 1104 20-21 0.35144436412959834 1104 22-23 0.40831987181016416 1104 24-25 0.4263725989240399 1104 26-27 0.4105387179507147 1104 28-29 0.4412273502753692 1104 30-31 0.42776889823594644 1104 32-33 0.4542092249803389 1104 34-35 0.46175061245659776 1104 36-37 0.421956502474643 1104 38-39 0.45412630253054687 1104 40-41 0.49946061802479846 1104 42-43 0.46218480687758046 1104 44-45 0.5225190757080682 1104 46-47 0.5208272842068595 1104 48-49 0.5245287530613751 1104 50-51 0.5221544699000589 1104 52-53 0.5021535261902272 1104 54-55 0.42339353941201097 1104 56-57 0.4095510256821271 1104 58-59 0.41500636327958773 1104 60-61 0.4228122595273476 1104 62-63 0.39173352340476697 1104 64-65 0.38871321192912944 1104 66-67 0.3698312092463496 1104 68-69 0.3685708285700571 1104 70-71 0.3952707142509766 1104 72-73 0.4261125094635574 1104 74-75 0.40761554303371383 1104 76-77 0.4109150486297466 1104 78-79 0.3896978772628401 1104 80-81 0.3532372570294129 1104 82-83 0.3237401971529934 1104 84-85 0.37004804230664945 1104 86-87 0.4198447051344445 1104 88-89 0.3899271995113196 1104 90-91 0.3931008294192182 1104 92-93 0.44230529416670805 1104 94-95 0.3716476862407063 1104 96-97 0.4521028481492877 1104 98-99 0.49666990939304867 1104 100 0.49010534807236183 1105 1 0.26870727141680817 1105 2 0.24074861037519923 1105 3 0.31224256361263514 1105 4 0.2530457951993128 1105 5 0.20741170912217655 1105 6 0.20905499943756922 1105 7 0.16963181220343415 1105 8 0.22732322439517816 1105 9 0.247737877969314 1105 10-11 0.2317500534103445 1105 12-13 0.21760525048652113 1105 14-15 0.24428241078680202 1105 16-17 0.26165144267591955 1105 18-19 0.23423907774406416 1105 20-21 0.23877150457438034 1105 22-23 0.27948486516872606 1105 24-25 0.24352690110333697 1105 26-27 0.2866689084866181 1105 28-29 0.2890061500027201 1105 30-31 0.3008633378724852 1105 32-33 0.32113015517597177 1105 34-35 0.2511817922575901 1105 36-37 0.3015651642056625 1105 38-39 0.28762342545071107 1105 40-41 0.20461572146839302 1105 42-43 0.24768542192904874 1105 44-45 0.2818772292504903 1105 46-47 0.22441033900445007 1105 48-49 0.22058116038585496 1105 50-51 0.25487811883779443 1105 52-53 0.18671231467120464 1105 54-55 0.25049495945780365 1105 56-57 0.2872256460227689 1105 58-59 0.18971914756050978 1105 60-61 0.21633569038122857 1105 62-63 0.20849177891437165 1105 64-65 0.25164237664327516 1105 66-67 0.18698807389923644 1105 68-69 0.2276021530563952 1105 70-71 0.25293963125754715 1105 72-73 0.17989645448373892 1105 74-75 0.2155985499419586 1105 76-77 0.25074044090880676 1105 78-79 0.15515213620376045 1105 80-81 0.17260628779055054 1105 82-83 0.16726357598673758 1105 84-85 0.1598876861775409 1105 86-87 0.19559148604145093 1105 88-89 0.18569130158377334 1105 90-91 0.16841866573433606 1105 92-93 0.1445869784493823 1105 94-95 0.20228681300827844 1105 96-97 0.24562898597715233 1105 98-99 0.26346521865605865 1105 100 0.3415459418233091 1106 1 0.19397282392927195 1106 2 0.22570839733456438 1106 3 0.30898364942021317 1106 4 0.2362302009436661 1106 5 0.2219474158259942 1106 6 0.21319626588407914 1106 7 0.2321444417438272 1106 8 0.19635267122715305 1106 9 0.22203203631776347 1106 10-11 0.2676367555555501 1106 12-13 0.2175050902008664 1106 14-15 0.30233894790990945 1106 16-17 0.28921314236092144 1106 18-19 0.28515947740112324 1106 20-21 0.3291952084988239 1106 22-23 0.30594401692791706 1106 24-25 0.28297625606232657 1106 26-27 0.28293926102746525 1106 28-29 0.29337796943913474 1106 30-31 0.29746659378260176 1106 32-33 0.3177172682703713 1106 34-35 0.32953446618680005 1106 36-37 0.29487978452663555 1106 38-39 0.36258352559347173 1106 40-41 0.3306130171406778 1106 42-43 0.2633619849478208 1106 44-45 0.3201607790770211 1106 46-47 0.27464785831742233 1106 48-49 0.29459766777917906 1106 50-51 0.3220044362886654 1106 52-53 0.3343191645980994 1106 54-55 0.3244805135266873 1106 56-57 0.2932253803643974 1106 58-59 0.3007309642841278 1106 60-61 0.2913851189711849 1106 62-63 0.28846279521421536 1106 64-65 0.27949194589564286 1106 66-67 0.2928965342604357 1106 68-69 0.253831892499484 1106 70-71 0.2916019851673468 1106 72-73 0.3126594515218599 1106 74-75 0.2532054155645227 1106 76-77 0.24911029940831497 1106 78-79 0.29378134487033947 1106 80-81 0.2390431637415915 1106 82-83 0.29819564555813116 1106 84-85 0.26568812016650156 1106 86-87 0.3466428697280506 1106 88-89 0.3264786833442628 1106 90-91 0.2838145755445254 1106 92-93 0.3134567546743696 1106 94-95 0.35666559694268685 1106 96-97 0.39546672923451 1106 98-99 0.43996174983963243 1106 100 0.43285992796614536 1107 1 0.16247171025782237 1107 2 0.17700196520287648 1107 3 0.23493638176331189 1107 4 0.19846836365740472 1107 5 0.18791988641103075 1107 6 0.18806424375551245 1107 7 0.167110952375765 1107 8 0.19260223549188282 1107 9 0.1712187958507485 1107 10-11 0.17270475289360832 1107 12-13 0.1224589192387171 1107 14-15 0.182931355477443 1107 16-17 0.16719764603016074 1107 18-19 0.20487143375063255 1107 20-21 0.25289980148811964 1107 22-23 0.2097860136593397 1107 24-25 0.26659345866451645 1107 26-27 0.23412757336250678 1107 28-29 0.2472181836171714 1107 30-31 0.2856764178587241 1107 32-33 0.2720240587235736 1107 34-35 0.20897599504039732 1107 36-37 0.31514373824136754 1107 38-39 0.29433422503347373 1107 40-41 0.357026355619837 1107 42-43 0.281807026846316 1107 44-45 0.3306122008189618 1107 46-47 0.3110384027488422 1107 48-49 0.2920507385244733 1107 50-51 0.3758460368971086 1107 52-53 0.33972482847757135 1107 54-55 0.3924446055449309 1107 56-57 0.34160840638175927 1107 58-59 0.3433966625244338 1107 60-61 0.31646125946581805 1107 62-63 0.28371356369278544 1107 64-65 0.3754575465765271 1107 66-67 0.3738333707544612 1107 68-69 0.42849708370238204 1107 70-71 0.41792814829757674 1107 72-73 0.3747928962977838 1107 74-75 0.3291856218653635 1107 76-77 0.3499736777261262 1107 78-79 0.33622959989205015 1107 80-81 0.37071783586494433 1107 82-83 0.3831481787079625 1107 84-85 0.3817125394502199 1107 86-87 0.4265978871348324 1107 88-89 0.36746326397452833 1107 90-91 0.39848665928718674 1107 92-93 0.3402359410151412 1107 94-95 0.3878887171694032 1107 96-97 0.36996744451942476 1107 98-99 0.4089688053557019 1107 100 0.386669725558626 1108 1 0.13586735043960374 1108 2 0.13879586534014265 1108 3 0.2355461619870951 1108 4 0.14889585500263536 1108 5 0.17991032365516446 1108 6 0.17456166176899046 1108 7 0.15673566962229302 1108 8 0.1850328385285991 1108 9 0.16235294633624164 1108 10-11 0.1919990148156714 1108 12-13 0.1405996455820997 1108 14-15 0.11607679460682618 1108 16-17 0.17110060056261034 1108 18-19 0.14905764113157005 1108 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-0.8054672709012394 2215 36-37 -0.8345971918393289 2215 38-39 -0.7995408409562614 2215 40-41 -0.7992900307902104 2215 42-43 -0.770842248507627 2215 44-45 -0.84928484102754 2215 46-47 -0.6914672592214188 2215 48-49 -0.7051308566126053 2215 50-51 -0.7136639101275009 2215 52-53 -0.8656734987662347 2215 54-55 -0.8003833217869172 2215 56-57 -0.7315918121408842 2215 58-59 -0.7415824828492816 2215 60-61 -0.8701861157577611 2215 62-63 -0.8639885910009824 2215 64-65 -0.7692369679849662 2215 66-67 -0.6595359084257026 2215 68-69 -0.6202285862308443 2215 70-71 -0.7075229492004098 2215 72-73 -0.6707192662138723 2215 74-75 -0.7347559875044141 2215 76-77 -0.7003088852755113 2215 78-79 -0.6793066759317838 2215 80-81 -0.6693820633569132 2215 82-83 -0.6728471714666071 2215 84-85 -0.6394382897933895 2215 86-87 -0.6125747628089186 2215 88-89 -0.64079847233808 2215 90-91 -0.6317803798347228 2215 92-93 -0.8048267942840219 2215 94-95 -0.7826424999136492 2215 96-97 -0.8116853096184045 2215 98-99 -0.7804777784897396 2215 100 -0.8233760258131362 2216 1 -0.6773525591557608 2216 2 -0.9847778288938365 2216 3 -0.7084965727965589 2216 4 -1.159680672281091 2216 5 -0.6289276751483044 2216 6 -0.8106355102262768 2216 7 -0.5327180863078027 2216 8 -0.5320842297005228 2216 9 -0.5978442415571692 2216 10-11 -0.8420457499846776 2216 12-13 -0.66811542168508 2216 14-15 -0.8983129712827207 2216 16-17 -0.9134415183268487 2216 18-19 -0.9569686538378406 2216 20-21 -0.7540822771292781 2216 22-23 -0.7039036149950348 2216 24-25 -0.831580507811033 2216 26-27 -0.9718886632048154 2216 28-29 -1.2493792132956685 2216 30-31 -1.2849467893398128 2216 32-33 -1.023524790827004 2216 34-35 -1.023681869526179 2216 36-37 -1.0159205658777424 2216 38-39 -1.1021522517505034 2216 40-41 -0.9755475637397097 2216 42-43 -1.6018988732654407 2216 44-45 -1.3397567774730703 2216 46-47 -1.0747771928793526 2216 48-49 -1.0530261580244655 2216 50-51 -1.2272361254564572 2216 52-53 -1.2027094556532774 2216 54-55 -1.3632833338420909 2216 56-57 -1.4488703184642375 2216 58-59 -1.1645388015249623 2216 60-61 -1.3882729330281478 2216 62-63 -1.4226200354970757 2216 64-65 -1.374239234252741 2216 66-67 -1.243807009852894 2216 68-69 -1.6109640695267018 2216 70-71 -1.3617149479678687 2216 72-73 -1.2043289034759184 2216 74-75 -1.265421477080551 2216 76-77 -1.3830960770284406 2216 78-79 -1.4187839726314166 2216 80-81 -1.2445219569169055 2216 82-83 -1.3119211198806333 2216 84-85 -1.3997359806920109 2216 86-87 -1.4891328679669016 2216 88-89 -1.3317818851530987 2216 90-91 -1.1829148385634696 2216 92-93 -1.2526107739555208 2216 94-95 -1.3069032342405578 2216 96-97 -1.4374321470649747 2216 98-99 -1.409019819906547 2216 100 -1.36479611850989 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 312.0 3 37.0 4 62.0 5 118.0 6 405.0 7 1042.0 8 2345.0 9 3931.0 10 5607.0 11 7717.0 12 11356.0 13 16379.0 14 17491.0 15 20724.0 16 25921.0 17 35730.0 18 36175.0 19 20119.0 20 14908.0 21 14318.0 22 14932.0 23 16305.0 24 19098.0 25 23205.0 26 29362.0 27 37511.0 28 48760.0 29 63659.0 30 81881.0 31 104656.0 32 134676.0 33 175982.0 34 236901.0 35 342570.0 36 552905.0 37 1020503.0 38 1380972.0 39 540046.0 40 1710.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.735175742868904 20.099014912942252 14.09771142948554 23.0680979147033 2 16.29313956429633 20.717681885730546 39.34454738040282 23.644631169570303 3 18.092195945285003 25.51629527791759 30.559799349093964 25.83170942770344 4 12.781618992278199 17.907023813551426 37.7042654594787 31.60709173469168 5 12.344170032941106 36.7235328421975 34.897276672286296 16.035020452575104 6 29.643749887604077 37.945831390901255 17.895051160647217 14.515367560847448 7 25.96474420349183 32.58518069272544 22.675176781914068 18.77489832186867 8 24.698423877805624 34.85995283707726 21.19970017771565 19.241923107401472 9 24.21966551588533 16.85407868656787 22.603585141680483 36.32267065586632 10-11 23.385189624947458 26.701909420549764 28.84702601470141 21.06587493980137 12-13 24.618466050481494 24.383016955714048 29.08310614951062 21.915410844293834 14-15 21.88392851849583 26.442317003041303 27.289534477000508 24.384220001462356 16-17 20.460035124184564 29.52466943367934 27.751366461996263 22.263928980139834 18-19 20.171407759690027 27.375373271037013 31.256769567050057 21.196449402222896 20-21 21.593982686112824 26.975982401151228 30.818389152804432 20.611645759931516 22-23 21.078818757112924 27.105697236010844 30.926030332798387 20.889453674077842 24-25 20.864060078283416 26.880978734395043 31.14122376579714 21.113737421524405 26-27 20.689387710013435 26.701049792987853 31.26705545546329 21.342507041535423 28-29 20.836707624534565 26.81523033514468 31.17286805068926 21.17519398963149 30-31 21.285154411244513 27.439481076090477 30.580420044748173 20.694944467916837 32-33 20.593192538826518 27.045064254702755 31.23286425984076 21.128878946629964 34-35 20.68614681529726 26.548727741327593 31.219835224217547 21.5452902191576 36-37 21.569814306613537 27.19345829353851 30.274106575241817 20.962620824606137 38-39 21.29245102741303 27.24729864508863 30.485001870431006 20.975248457067334 40-41 20.90832595733362 27.086844714308217 30.646582209740824 21.358247118617342 42-43 20.95078365427084 27.118354510801762 30.362134413736968 21.56872742119043 44-45 21.36699160588507 27.34595819917709 30.032314091706645 21.254736103231192 46-47 21.290168568024505 27.884381476231496 29.434625916763153 21.39082403898085 48-49 21.301254799340203 27.401507925074466 29.60811259184429 21.689124683741042 50-51 21.42020946851105 27.29101120065071 29.387860201239803 21.90091912959844 52-53 21.39469730339774 27.482144942692482 29.190679423934917 21.93247832997486 54-55 21.571800342704854 28.06074345729558 28.907348155683888 21.460108044315678 56-57 21.10245357467723 27.715153415853628 29.02435631186972 22.158036697599425 58-59 20.98286322943175 27.207452892036915 29.48980968091429 22.319874197617047 60-61 21.553244244299435 27.743876833353394 28.88611436682699 21.816764555520184 62-63 21.273865286677886 27.66450455513681 29.041084466609 22.020545691576302 64-65 21.23335410272569 27.333123070407844 29.38769222803805 22.045830598828417 66-67 21.1643566399115 27.453095459565784 29.00988097418924 22.372666926333476 68-69 21.805964866725123 27.6914889559596 28.757565463603076 21.744980713712206 70-71 21.73317318570663 27.348991597585215 28.936288950268274 21.98154626643988 72-73 21.356606909706105 27.522745053633844 29.237682278096038 21.882965758564016 74-75 21.400279942161887 27.451514535313994 29.017034656428603 22.131170866095516 76-77 21.490066558887154 27.665690248325653 28.60939333810377 22.234849854683418 78-79 21.64150818860934 27.81347708703122 28.701829646413806 21.84318507794563 80-81 21.38581014078562 27.480664258649355 28.92279694675315 22.210728653811877 82-83 21.17017151886502 27.562862650133845 29.02827450764952 22.238691323351613 84-85 21.369461800028496 27.505493217736156 28.79581394971989 22.329231032515462 86-87 21.540154343673798 27.638886432417802 28.675320712747915 22.145638511160488 88-89 21.18535596902019 27.49589552297796 28.780901158976256 22.537847349025597 90-91 21.017720691002705 27.387841992440215 28.754295053037577 22.840142263519507 92-93 21.26017294437728 27.631181763522793 28.563014328703005 22.545630963396913 94-95 21.13457241812432 27.3915408867548 28.738932955278884 22.73495373984199 96-97 21.001659772848853 27.404037403877336 28.832046757415668 22.762256065858143 98-99 21.00400774297405 27.396042095677764 28.74150784054675 22.85844232080144 100 21.170669915508377 27.46069622835958 28.535995644496882 22.83263821163516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1201.0 1 898.0 2 2467.5 3 3874.0 4 2181.5 5 986.0 6 1687.5 7 3773.5 8 6065.5 9 6900.5 10 5516.5 11 3822.5 12 3621.5 13 3992.0 14 4478.5 15 5081.5 16 5709.0 17 6327.5 18 7068.5 19 7946.5 20 9320.0 21 11173.0 22 13722.5 23 17757.0 24 22795.0 25 28892.5 26 37375.5 27 48217.5 28 61714.0 29 76634.5 30 90130.0 31 103192.5 32 115512.5 33 126712.0 34 138029.5 35 148194.5 36 156008.5 37 162458.5 38 168071.5 39 170984.5 40 172685.0 41 172631.5 42 169114.5 43 166392.5 44 164310.5 45 161337.0 46 159309.0 47 157318.5 48 155169.0 49 155888.5 50 155982.0 51 150746.5 52 142256.0 53 134781.0 54 129543.5 55 124289.5 56 116508.0 57 106627.0 58 97960.0 59 89913.5 60 78956.0 61 65857.5 62 54248.5 63 43898.5 64 34905.0 65 27657.5 66 21369.5 67 16167.0 68 12090.0 69 9024.5 70 6573.5 71 4694.0 72 3535.5 73 2667.5 74 1947.5 75 1388.5 76 894.5 77 599.5 78 447.5 79 322.0 80 213.0 81 106.5 82 44.5 83 20.0 84 12.0 85 7.0 86 7.0 87 4.0 88 2.5 89 3.0 90 1.5 91 0.0 92 0.5 93 0.5 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06151771494789571 2 2.173770846215396E-4 3 0.0 4 0.11738362569563138 5 0.0017390166769723169 6 0.001857585995856793 7 0.0 8 0.0 9 1.976155314741269E-5 10-11 0.0 12-13 2.9642329721119035E-5 14-15 1.976155314741269E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.778539783267142E-4 30-31 2.56900190916365E-4 32-33 1.976155314741269E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.667809674900713E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.778539783267142E-4 80-81 2.56900190916365E-4 82-83 2.56900190916365E-4 84-85 0.0 86-87 9.880776573706345E-6 88-89 5.928465944223807E-5 90-91 6.916543601594441E-5 92-93 6.620120304383251E-4 94-95 7.311774664542695E-4 96-97 0.0 98-99 2.9642329721119035E-5 100 0.0013042625077292374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5060331.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.69406265372717 #Duplication Level Percentage of deduplicated Percentage of total 1 78.95873665421365 51.08161456166842 2 13.193501582372773 17.070824359841456 3 3.6845645017391244 7.151083401816298 4 1.471663347226995 3.808315231627883 5 0.7847576162007048 2.538457919523899 6 0.4346755569919732 1.687255663084948 7 0.30860373058750895 1.397538035726157 8 0.21049177464007213 1.0894054445327237 9 0.15744853365887182 0.9167386771138073 >10 0.7428288918841097 8.340570802913273 >50 0.03622037392148999 1.599195403797751 >100 0.015498328776083025 1.7088810011691262 >500 5.198448163563265E-4 0.22542550585242954 >1k 3.669472277981157E-4 0.45088856991274734 >5k 6.115787129646455E-5 0.30109354307981884 >10k+ 6.115787129646455E-5 0.6327118783391489 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16518 0.3264213348889628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15465 0.30561241942473727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9340 0.18457290639683452 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5880 0.11619793250678662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.976155314741269E-5 0.0 9 0.0 0.0 0.0 3.952310629482538E-5 0.0 10-11 0.0 0.0 0.0 4.940388286853173E-5 0.0 12-13 0.0 0.0 0.0 7.904621258965076E-5 0.0 14-15 0.0 9.880776573706345E-6 0.0 9.880776573706344E-5 0.0 16-17 0.0 1.976155314741269E-5 0.0 9.880776573706344E-5 0.0 18-19 0.0 1.976155314741269E-5 0.0 9.880776573706344E-5 0.0 20-21 0.0 1.976155314741269E-5 0.0 1.1856931888447613E-4 0.0 22-23 0.0 1.976155314741269E-5 0.0 2.8654252063748395E-4 0.0 24-25 0.0 1.976155314741269E-5 0.0 7.707005727490949E-4 0.0 26-27 0.0 1.976155314741269E-5 0.0 0.0015018780392033644 0.0 28-29 0.0 3.952310629482538E-5 0.0 0.0037052912151398793 0.0 30-31 0.0 3.952310629482538E-5 0.0 0.010246365306933479 0.0 32-33 0.0 5.928465944223807E-5 0.0 0.025907396176258035 1.976155314741269E-5 34-35 0.0 5.928465944223807E-5 0.0 0.0495224521874162 1.976155314741269E-5 36-37 0.0 6.916543601594441E-5 0.0 0.08275150380479063 1.976155314741269E-5 38-39 0.0 9.880776573706346E-5 0.0 0.13040648921977632 1.976155314741269E-5 40-41 0.0 1.3833087203188882E-4 0.0 0.18791260887874725 1.976155314741269E-5 42-43 0.0 1.3833087203188882E-4 0.0 0.24867938480704127 1.976155314741269E-5 44-45 0.0 1.3833087203188882E-4 0.0 0.3181906480030654 1.976155314741269E-5 46-47 0.0 1.4821164860559516E-4 0.0 0.3994896776515212 1.976155314741269E-5 48-49 0.0 1.5809242517930152E-4 0.0 0.4893454598128067 1.976155314741269E-5 50-51 0.0 1.778539783267142E-4 0.0 0.5838649685168816 1.976155314741269E-5 52-53 0.0 1.778539783267142E-4 0.0 0.678335073338088 1.976155314741269E-5 54-55 0.0 1.778539783267142E-4 0.0 0.7856403069285389 1.976155314741269E-5 56-57 0.0 1.9761553147412689E-4 0.0 0.9090116832278363 1.976155314741269E-5 58-59 0.0 2.1737708462153958E-4 0.0 1.0375013017923136 1.976155314741269E-5 60-61 0.0 2.2725786119524594E-4 0.0 1.1753480157720908 1.976155314741269E-5 62-63 0.0 2.3713863776895228E-4 0.0 1.3134713914959317 1.976155314741269E-5 64-65 0.0 2.3713863776895228E-4 0.0 1.466939613238739 1.976155314741269E-5 66-67 0.0 2.3713863776895228E-4 0.0 1.6452382265112697 1.976155314741269E-5 68-69 0.0 2.3713863776895228E-4 0.0 1.8371426691257944 1.976155314741269E-5 70-71 0.0 2.3713863776895228E-4 0.0 2.0412103477025516 1.976155314741269E-5 72-73 0.0 2.3713863776895228E-4 0.0 2.265671949127438 1.976155314741269E-5 74-75 0.0 2.3713863776895228E-4 0.0 2.4880190643655524 1.976155314741269E-5 76-77 0.0 2.3713863776895228E-4 0.0 2.7230432159477314 1.976155314741269E-5 78-79 0.0 2.3713863776895228E-4 0.0 2.984231663897085 1.976155314741269E-5 80-81 0.0 2.3713863776895228E-4 0.0 3.2648457185903452 1.976155314741269E-5 82-83 0.0 2.470194143426586E-4 0.0 3.5600240379532484 1.976155314741269E-5 84-85 0.0 2.6678096749007134E-4 0.0 3.855884130899738 1.976155314741269E-5 86-87 0.0 2.964232972111903E-4 0.0 4.1680672667459895 1.976155314741269E-5 88 0.0 3.1618485035860304E-4 0.0 4.420738485288808 1.976155314741269E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9665 0.0 54.59304 1 GTATCAA 17680 0.0 42.56962 1 ATCAACG 22025 0.0 33.705868 3 TCAACGC 22365 0.0 33.214493 4 TATCAAC 22715 0.0 32.702717 2 GTGGTAT 4160 0.0 32.246685 1 CAACGCA 23165 0.0 32.13719 5 AACGCAG 24100 0.0 31.006771 6 TGGTATC 4420 0.0 29.376581 2 ACGCAGA 27260 0.0 27.308731 7 CGCAGAG 27360 0.0 27.054325 8 TACCTGG 3620 0.0 26.121729 2 GTACCTG 3900 0.0 25.103386 1 GCAGAGT 29520 0.0 24.740408 9 ATAACGC 540 0.0 23.522552 3 TATAACG 570 0.0 23.109875 2 TCTGTCG 1750 0.0 23.095778 8 GTACATG 12590 0.0 22.61847 1 AGAGTAC 27820 0.0 21.386948 10-11 GAGTACT 22565 0.0 21.369 12-13 >>END_MODULE