FastQCFastQC Report
Wed 25 May 2016
SRR2049507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172340
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98650.034.762991
GTATCAA148550.031.0105361
TCAACGC149850.029.8822354
ATCAACG152250.029.478593
CAACGCA166300.026.9877325
TATCAAC174200.026.146062
AACGCAG174600.025.6455576
ACGCAGA198900.022.1256457
GTACATG208450.022.0993791
CGCAGAG203000.021.2379638
TACATGG208450.020.6716252
ACATGGG214550.019.105943
GCAGAGT238750.017.3188389
GTGGTAT71500.016.679741
AGAGTAC193500.016.27230310-11
GAGTACA164950.016.1978591
TGGTATC74450.015.8785812
TATACCG6051.8189894E-1215.2256625
CAGAGTA233200.015.12196710-11
CATGGGG152900.015.061454
Produced by FastQC (version 0.11.5)