FastQCFastQC Report
Wed 25 May 2016
SRR2049507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172340
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221240.24120344426831103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214490.2338443625072773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120930.13184203812767514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177900.049.286161
GTATCAA295650.037.588531
ATCAACG361350.030.4337523
TCAACGC364000.030.2380434
TATCAAC373850.029.4035872
CAACGCA376500.029.2726885
AACGCAG389700.028.2930626
ACGCAGA439350.024.9559787
CGCAGAG442100.024.662558
GTGGTAT90550.023.141571
GCAGAGT476300.022.3983369
GTACATG209900.021.9629921
GAGTACT344550.021.46261412-13
TGGTATC93200.021.3069532
TACATGG213300.020.2965322
AGAGTAC428850.020.28472710-11
CAGAGTA472800.019.81558610-11
GTACTTT380550.019.27170814-15
ACATGGG217600.019.2034363
AGTACTT363750.018.86331412-13
Produced by FastQC (version 0.11.5)