FastQCFastQC Report
Wed 25 May 2016
SRR2049506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7738131
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73750.048.0269131
GTATCAA127300.037.0315441
ATCAACG137950.033.3134043
TCAACGC137950.033.239214
CAACGCA153450.029.9815435
TATCAAC154850.029.9092082
AACGCAG165200.027.848476
GTACATG246700.024.378631
ACGCAGA191700.023.6784467
GTGGTAT40050.023.3877871
TACATGG250550.023.1421152
CGCAGAG191400.022.9744648
ACATGGG257400.021.9495143
TATACCG5700.020.649725
TGGTATC44700.019.8064252
CATGGGG187950.019.4683044
GCAGAGT219750.019.289989
GAGTACA190450.017.7379531
CAGAGTA207600.017.0848510-11
AGAGTAC193200.017.07036610-11
Produced by FastQC (version 0.11.5)