##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049506_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7738131 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.696506688759857 31.0 31.0 34.0 27.0 34.0 2 30.950192365572512 31.0 31.0 34.0 28.0 34.0 3 31.045851640402574 31.0 31.0 34.0 27.0 34.0 4 34.62430760089226 37.0 35.0 37.0 32.0 37.0 5 34.60066300247437 37.0 35.0 37.0 32.0 37.0 6 34.65205396496906 37.0 35.0 37.0 32.0 37.0 7 30.715674624789887 37.0 33.0 37.0 2.0 37.0 8 32.504004390724326 37.0 33.0 37.0 17.0 37.0 9 35.2772515740558 39.0 34.0 39.0 28.0 39.0 10-11 35.81737146864016 39.0 36.0 39.0 30.0 39.0 12-13 35.96178024124947 39.0 37.0 39.0 30.5 39.0 14-15 37.14600767291223 40.0 37.0 41.0 31.0 41.0 16-17 37.15776606780112 40.0 37.0 41.0 31.0 41.0 18-19 37.15029527155847 40.0 37.0 41.0 31.0 41.0 20-21 37.21181658723535 40.0 37.0 41.0 31.0 41.0 22-23 37.16881014291435 40.0 37.0 41.0 31.0 41.0 24-25 37.13781887383401 40.0 37.0 41.0 31.0 41.0 26-27 37.15906502487487 40.0 37.0 41.0 31.0 41.0 28-29 37.14269621437011 40.0 37.0 41.0 31.0 41.0 30-31 37.06181027434144 40.0 37.0 41.0 30.0 41.0 32-33 37.013520835974475 40.0 37.0 41.0 30.0 41.0 34-35 36.95230198351514 40.0 37.0 41.0 30.0 41.0 36-37 36.77726074939801 40.0 37.0 41.0 30.0 41.0 38-39 36.66374561247412 40.0 36.5 41.0 30.0 41.0 40-41 36.57888733080378 40.0 36.0 41.0 30.0 41.0 42-43 36.4564639704342 40.0 36.0 41.0 30.0 41.0 44-45 36.32848177421654 40.0 36.0 41.0 29.5 41.0 46-47 36.158951302323516 40.0 35.0 41.0 29.0 41.0 48-49 36.064420336125096 39.5 35.0 41.0 29.0 41.0 50-51 35.915963945298934 39.0 35.0 41.0 28.0 41.0 52-53 35.780904006406715 39.0 35.0 41.0 28.0 41.0 54-55 35.64072325733436 39.0 35.0 41.0 28.0 41.0 56-57 35.42477085228978 39.0 35.0 41.0 28.0 41.0 58-59 35.225503871671336 38.5 34.5 40.0 28.0 41.0 60-61 34.9884160012282 38.0 34.0 40.0 27.0 41.0 62-63 34.73909319963697 38.0 34.0 40.0 27.0 41.0 64-65 34.37260257031058 37.0 34.0 40.0 26.0 41.0 66-67 30.907599199341547 36.0 30.0 39.5 6.5 41.0 68-69 31.252107970257935 35.0 30.0 39.0 11.0 41.0 70-71 31.404650489892198 35.0 30.0 39.0 15.5 41.0 72-73 32.620588421157514 35.0 31.5 39.0 23.5 40.0 74-75 32.700305926586154 35.0 33.0 37.5 24.0 40.0 76-77 32.41322672102605 35.0 33.0 37.0 23.5 39.0 78-79 29.10006712215133 35.0 29.0 36.0 6.5 39.0 80-81 30.868822458549744 35.0 29.5 36.0 18.5 38.5 82-83 31.28701698123229 35.0 31.5 36.0 20.0 37.0 84-85 29.48735915688168 34.5 30.0 35.5 11.0 37.0 86-87 29.13256560272758 34.0 28.0 35.0 10.5 36.5 88-89 30.38343787408096 34.0 30.5 35.0 18.0 36.0 90-91 30.544887195629023 35.0 31.5 35.0 17.5 36.0 92-93 30.40884698126718 35.0 32.0 35.0 10.0 36.0 94-95 30.21439834761133 35.0 32.0 35.0 4.5 35.5 96-97 30.03539155643656 35.0 31.5 35.0 2.0 35.0 98-99 29.852552056820954 35.0 31.0 35.0 2.0 35.0 100 29.648427249422372 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.285581336353566 1101 2 0.31256851842579536 1101 3 0.34713959592603416 1101 4 0.32082953583002194 1101 5 0.26889834424078884 1101 6 0.33852856375809637 1101 7 -12.16972640041417 1101 8 -6.575211360583445 1101 9 -2.17179440130991 1101 10-11 -0.11334013315834568 1101 12-13 0.30930033499091536 1101 14-15 0.49606543522425994 1101 16-17 0.5614040313078164 1101 18-19 0.5786127548139746 1101 20-21 0.5368008251910936 1101 22-23 0.5158987712623855 1101 24-25 0.5586294342415599 1101 26-27 0.538270244083904 1101 28-29 0.474598050052343 1101 30-31 0.542581269266428 1101 32-33 0.5975117904692127 1101 34-35 0.578120261936192 1101 36-37 0.6039932721976911 1101 38-39 0.6305383157925206 1101 40-41 0.5651616788679732 1101 42-43 0.5469283488897787 1101 44-45 0.44363422673271913 1101 46-47 0.4623699660884597 1101 48-49 0.42314239099945894 1101 50-51 0.495386143241646 1101 52-53 0.4625464961871799 1101 54-55 0.36441068845625324 1101 56-57 0.3366747449240819 1101 58-59 0.3517107138957769 1101 60-61 0.3566711112362455 1101 62-63 0.33139063832366844 1101 64-65 0.1249698586530883 1101 66-67 -10.313338272587021 1101 68-69 -8.219396083040156 1101 70-71 -6.73895712359921 1101 72-73 -1.6561155977062363 1101 74-75 -0.40279468852470757 1101 76-77 -0.05722102100391169 1101 78-79 3.0316346618424745 1101 80-81 0.9503284959105152 1101 82-83 0.2873149033188831 1101 84-85 -5.423601700518805 1101 86-87 -6.026316774535566 1101 88-89 -1.4636008601262667 1101 90-91 -0.18229566966796185 1101 92-93 0.18530839284040468 1101 94-95 0.2649606432300864 1101 96-97 0.32686800042482744 1101 98-99 0.3072511231085606 1101 100 0.3561180184452297 1102 1 0.17328149151880723 1102 2 0.21751141121781004 1102 3 0.17779132993761593 1102 4 0.1792080590408247 1102 5 0.14823257337346263 1102 6 0.21752526849623166 1102 7 -12.275532699803104 1102 8 -6.696559178672196 1102 9 -2.4330268852335095 1102 10-11 -0.3452273904127168 1102 12-13 0.22309194567012014 1102 14-15 0.388518425754377 1102 16-17 0.39118286085000165 1102 18-19 0.3902768420584266 1102 20-21 0.40954972943654155 1102 22-23 0.3720055033544014 1102 24-25 0.3682095882870513 1102 26-27 0.4060319654583253 1102 28-29 0.33390166604236526 1102 30-31 0.3207437608683108 1102 32-33 0.30959878298289567 1102 34-35 0.3266452923293883 1102 36-37 0.47184527334943027 1102 38-39 0.4739977808723381 1102 40-41 0.3616083748080854 1102 42-43 0.32317606940038957 1102 44-45 0.3248075439096638 1102 46-47 0.31351550948673435 1102 48-49 0.1969934483626048 1102 50-51 0.25634268897540835 1102 52-53 0.1947250881825724 1102 54-55 0.12884275567319747 1102 56-57 0.2044763573628927 1102 58-59 0.1655380165799798 1102 60-61 0.09711665143524328 1102 62-63 0.07962553370710168 1102 64-65 -0.033395967401872895 1102 66-67 -10.3933023606313 1102 68-69 -6.876295137651923 1102 70-71 -6.410318238709426 1102 72-73 -1.336400113974591 1102 74-75 -0.4108310883711397 1102 76-77 -0.2568664004389234 1102 78-79 2.8378658660527307 1102 80-81 0.7665414642056128 1102 82-83 0.10402695981822774 1102 84-85 -5.672422964555661 1102 86-87 -6.177391333834283 1102 88-89 -1.6545755817886167 1102 90-91 -0.3739174463899779 1102 92-93 -0.024016118469063485 1102 94-95 -0.004663660925782409 1102 96-97 0.10646757012121455 1102 98-99 0.15201239658330223 1102 100 0.2548770964117679 1103 1 0.13055580593577432 1103 2 0.16692675157324643 1103 3 0.18743695736403154 1103 4 0.14418590229635697 1103 5 0.14842359312505948 1103 6 0.21715482938281383 1103 7 -12.109307814646964 1103 8 -6.60958573969798 1103 9 -2.3263733850092407 1103 10-11 -0.36514487297345966 1103 12-13 0.19673262166350014 1103 14-15 0.35380614442172487 1103 16-17 0.37909414300241906 1103 18-19 0.3920714706912847 1103 20-21 0.3615968115518342 1103 22-23 0.3809249947159685 1103 24-25 0.46561926958568023 1103 26-27 0.41143924547625943 1103 28-29 0.4020042192881448 1103 30-31 0.3715238870805493 1103 32-33 0.4095871342581603 1103 34-35 0.37654422393349307 1103 36-37 0.46297310191114605 1103 38-39 0.4902607539749013 1103 40-41 0.4516371147737459 1103 42-43 0.4353197440945209 1103 44-45 0.40318253230928036 1103 46-47 0.4162853925548049 1103 48-49 0.40633524224688244 1103 50-51 0.40371355730324865 1103 52-53 0.33259021045067527 1103 54-55 0.39079130214435764 1103 56-57 0.4007211178535073 1103 58-59 0.45689224608209145 1103 60-61 0.38756112036940493 1103 62-63 0.31312953777496944 1103 64-65 0.19235423947174013 1103 66-67 -10.152699643046656 1103 68-69 -8.131095862289605 1103 70-71 -6.619900789016452 1103 72-73 -1.5201260721667893 1103 74-75 -0.36756559798399735 1103 76-77 -0.05241296247007199 1103 78-79 3.085927537591566 1103 80-81 1.00796384599764 1103 82-83 0.317724990072044 1103 84-85 -5.3883453695484675 1103 86-87 -5.863723754603619 1103 88-89 -1.3391960267022327 1103 90-91 -0.056982085695189255 1103 92-93 0.3022963689638516 1103 94-95 0.35278702775068993 1103 96-97 0.4661464519983127 1103 98-99 0.44394369714166615 1103 100 0.5461959447250706 1104 1 0.21649840855736713 1104 2 0.24786985630851177 1104 3 0.2534416917825091 1104 4 0.268339805084608 1104 5 0.2509673270396249 1104 6 0.3090036506969511 1104 7 -12.223179175575424 1104 8 -6.582259820273869 1104 9 -2.3153642209151073 1104 10-11 -0.21717069843080594 1104 12-13 0.35237338323576495 1104 14-15 0.5181137997829453 1104 16-17 0.5094740497752497 1104 18-19 0.5274045031286931 1104 20-21 0.5345736797008769 1104 22-23 0.5344831052757257 1104 24-25 0.6107137361379102 1104 26-27 0.5700483876616715 1104 28-29 0.5552934268038641 1104 30-31 0.5215323975737647 1104 32-33 0.5284165380595311 1104 34-35 0.4961000754625928 1104 36-37 0.6022375742557529 1104 38-39 0.6142664851440856 1104 40-41 0.5959350556237268 1104 42-43 0.5449329887518246 1104 44-45 0.522627354980628 1104 46-47 0.49198930100948246 1104 48-49 0.44716179533936895 1104 50-51 0.4903503976112731 1104 52-53 0.499581723100917 1104 54-55 0.48705811104709795 1104 56-57 0.4777990331445636 1104 58-59 0.40889851671710886 1104 60-61 0.35394079424959557 1104 62-63 0.24842112373039527 1104 64-65 0.16326819601981413 1104 66-67 -10.275720544253364 1104 68-69 -8.250053160042576 1104 70-71 -6.669566954779359 1104 72-73 -1.6169688071559065 1104 74-75 -0.4110842644668793 1104 76-77 -0.08107470280752693 1104 78-79 2.9717467299179106 1104 80-81 0.9404605031491222 1104 82-83 0.2821264415698934 1104 84-85 -5.4961154870809175 1104 86-87 -5.996306260473169 1104 88-89 -1.4762392238655018 1104 90-91 -0.1928290672288533 1104 92-93 0.10805002312439527 1104 94-95 0.14728620418809868 1104 96-97 0.24815930083429905 1104 98-99 0.26972488487674084 1104 100 0.3501862096453294 1105 1 0.026177678488430445 1105 2 0.0755790770267275 1105 3 0.04936899891056612 1105 4 0.07208339243842943 1105 5 0.06175782507474992 1105 6 0.1237603907440814 1105 7 -12.210037065179687 1105 8 -6.7074209419530995 1105 9 -2.485594247616625 1105 10-11 -0.44893449984623146 1105 12-13 0.08461318887547975 1105 14-15 0.22873601017597167 1105 16-17 0.24318138481631024 1105 18-19 0.25979533280239764 1105 20-21 0.24933083027278968 1105 22-23 0.2594069131411487 1105 24-25 0.31438344911163085 1105 26-27 0.23011922386092465 1105 28-29 0.24947813954234732 1105 30-31 0.269635453304538 1105 32-33 0.3225662580228743 1105 34-35 0.3239855503834548 1105 36-37 0.403546626962239 1105 38-39 0.3639714427707048 1105 40-41 0.4008868687160856 1105 42-43 0.38243821941014744 1105 44-45 0.2887068482550248 1105 46-47 0.2875871099435443 1105 48-49 0.25490340690883073 1105 50-51 0.3697755070410409 1105 52-53 0.352949240753226 1105 54-55 0.34382702357037687 1105 56-57 0.2803456321319544 1105 58-59 0.31096691606508386 1105 60-61 0.29056548123746495 1105 62-63 0.1837401745893743 1105 64-65 0.05798636116563216 1105 66-67 -10.20061167821368 1105 68-69 -8.261504071966105 1105 70-71 -6.7318321403698 1105 72-73 -1.77333591037155 1105 74-75 -0.6055636997858826 1105 76-77 -0.2957414338095816 1105 78-79 2.834205460092484 1105 80-81 0.7005165493007972 1105 82-83 0.06200410771451459 1105 84-85 -5.529951493379258 1105 86-87 -6.067849488263803 1105 88-89 -1.597374216743269 1105 90-91 -0.3497921825145802 1105 92-93 0.013258397841774183 1105 94-95 0.04532556313223779 1105 96-97 0.14072917940270813 1105 98-99 0.18683873474672907 1105 100 0.2713797833677951 1106 1 0.06431602014775706 1106 2 0.13610148534552025 1106 3 0.10546655127849647 1106 4 0.06483228421060261 1106 5 0.0631846481813767 1106 6 0.09349099494413338 1106 7 -12.22879123802075 1106 8 -6.751371331484258 1106 9 -2.601103674337068 1106 10-11 -0.5159932060918067 1106 12-13 0.07408280267348744 1106 14-15 0.18680727377611817 1106 16-17 0.18373958802199297 1106 18-19 0.21138261164083616 1106 20-21 0.20612246565233505 1106 22-23 0.22069013191129727 1106 24-25 0.28137780507129406 1106 26-27 0.2604688970209281 1106 28-29 0.2322399294901203 1106 30-31 0.2401533265245419 1106 32-33 0.27742944349480325 1106 34-35 0.3668270163014924 1106 36-37 0.3968325195068232 1106 38-39 0.31142304061188497 1106 40-41 0.30628799965311515 1106 42-43 0.253592475504675 1106 44-45 0.20457998381575493 1106 46-47 0.31940282494457506 1106 48-49 0.2242182853511281 1106 50-51 0.3223345676575633 1106 52-53 0.2877905862498338 1106 54-55 0.2575027085159789 1106 56-57 0.2885481793247351 1106 58-59 0.21454687387225135 1106 60-61 0.23311656640962752 1106 62-63 0.2000499783892522 1106 64-65 0.04326151786948884 1106 66-67 -10.288881988493369 1106 68-69 -8.279321112520908 1106 70-71 -6.8114299600850465 1106 72-73 -1.7747218111407719 1106 74-75 -0.5043962719350503 1106 76-77 -0.2289513588586516 1106 78-79 2.850803725125971 1106 80-81 0.7902826050848333 1106 82-83 0.11718821684426928 1106 84-85 -5.545211727094653 1106 86-87 -6.034891781818718 1106 88-89 -1.5385363561799963 1106 90-91 -0.26659778069607754 1106 92-93 0.05022923346385255 1106 94-95 0.06080488736693823 1106 96-97 0.1389146485312871 1106 98-99 0.21830523623778575 1106 100 0.2646645963660532 1107 1 0.21736216600589486 1107 2 0.25121438902953486 1107 3 0.22895247355374337 1107 4 0.2515143259438304 1107 5 0.2732528689929694 1107 6 0.27637079601421277 1107 7 -12.1046751078003 1107 8 -6.586412359680299 1107 9 -2.3660526918943177 1107 10-11 -0.35783277151401194 1107 12-13 0.23154364509800018 1107 14-15 0.3394324341752579 1107 16-17 0.4033526817441597 1107 18-19 0.44314466413429443 1107 20-21 0.39956800750334054 1107 22-23 0.43747231161002986 1107 24-25 0.5168891871063082 1107 26-27 0.4636587800548355 1107 28-29 0.47479606337054747 1107 30-31 0.4932413350013576 1107 32-33 0.47993930204636115 1107 34-35 0.48740156535711066 1107 36-37 0.5332636008943155 1107 38-39 0.5029704382294398 1107 40-41 0.5391704415746688 1107 42-43 0.5092385030493176 1107 44-45 0.5015922524213394 1107 46-47 0.5131277610755589 1107 48-49 0.46349090480508437 1107 50-51 0.49837440658527044 1107 52-53 0.47621161020992275 1107 54-55 0.4862444570225577 1107 56-57 0.4491187341360501 1107 58-59 0.4504929579028172 1107 60-61 0.4291228734236867 1107 62-63 0.3857728696968792 1107 64-65 0.2755318384374945 1107 66-67 -10.104277427042408 1107 68-69 -8.089014876763141 1107 70-71 -6.5796420252955805 1107 72-73 -1.5800811358890599 1107 74-75 -0.29381666407019225 1107 76-77 -0.012614489168008447 1107 78-79 3.1181936763355225 1107 80-81 1.053068445370318 1107 82-83 0.34632047891052054 1107 84-85 -5.381655940253758 1107 86-87 -5.901834825545507 1107 88-89 -1.412378061577062 1107 90-91 -0.13723715223559552 1107 92-93 0.13750617909114027 1107 94-95 0.22758011888716112 1107 96-97 0.3411393034872141 1107 98-99 0.3587838528960816 1107 100 0.4272067813372864 1108 1 -0.014336268810122732 1108 2 0.06830686156370547 1108 3 0.06112358290096154 1108 4 0.009569063555431967 1108 5 -0.011259125212546905 1108 6 0.04157582444717889 1108 7 -12.245367950085342 1108 8 -6.744259413701098 1108 9 -2.670961797486605 1108 10-11 -0.5940613061027307 1108 12-13 -0.019247735619245532 1108 14-15 0.1689156375653127 1108 16-17 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-1.1187761486120138 2216 14-15 -1.3251671895601191 2216 16-17 -1.4343294542022704 2216 18-19 -1.9182222480235964 2216 20-21 -1.5248629837934047 2216 22-23 -1.3427775947854599 2216 24-25 -1.3598927179855806 2216 26-27 -1.7668988386992197 2216 28-29 -1.984209349011408 2216 30-31 -1.9111005487391992 2216 32-33 -2.7310285015720126 2216 34-35 -1.8569430255268102 2216 36-37 -1.5224589987104054 2216 38-39 -1.7373787531050624 2216 40-41 -1.7774245145407974 2216 42-43 -1.4215804722346164 2216 44-45 -1.5479440166726803 2216 46-47 -1.689132716194905 2216 48-49 -1.797513035724748 2216 50-51 -2.22135213873446 2216 52-53 -1.980258580141701 2216 54-55 -1.6385166408167962 2216 56-57 -1.6616237830649396 2216 58-59 -1.9492131809316504 2216 60-61 -2.1398179429145614 2216 62-63 -1.9923274086550293 2216 64-65 -1.6632138876529794 2216 66-67 1.6857065849916744 2216 68-69 0.9089645309314598 2216 70-71 0.5331492753535976 2216 72-73 -1.2048846609849484 2216 74-75 -2.031420140610525 2216 76-77 -1.7057318695072041 2216 78-79 0.8097172071732182 2216 80-81 -0.6415068419848922 2216 82-83 -1.5520055736895415 2216 84-85 0.24394219147076157 2216 86-87 0.287262524287339 2216 88-89 -0.9777715822848556 2216 90-91 -1.320036974447202 2216 92-93 -1.378494012363923 2216 94-95 -1.3955509654228244 2216 96-97 -1.4293291857529837 2216 98-99 -1.4343573885586487 2216 100 -2.579612293999581 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 121239.0 3 24190.0 4 13960.0 5 12528.0 6 11496.0 7 12147.0 8 13494.0 9 15266.0 10 17185.0 11 19042.0 12 21237.0 13 24329.0 14 27942.0 15 30632.0 16 32688.0 17 33764.0 18 32037.0 19 29389.0 20 28064.0 21 28818.0 22 30930.0 23 35667.0 24 42527.0 25 52775.0 26 67324.0 27 82898.0 28 102910.0 29 130642.0 30 160376.0 31 197018.0 32 263572.0 33 370868.0 34 557558.0 35 761145.0 36 876733.0 37 1431596.0 38 1531867.0 39 491121.0 40 1157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94317117965306 20.13185771196482 14.760905624420213 23.164065483961906 2 15.655256086078067 19.935703535297534 39.93265102987811 24.476389348746288 3 18.723332105195617 24.719000496639765 29.823637959302935 26.73402943886168 4 13.067643223251881 16.8061590576403 37.95006343422026 32.17613428488756 5 13.711990167957596 37.6041504452398 34.78904337251461 13.894816014287995 6 31.370453124157947 37.04284401807074 17.62037929838832 13.966323559382992 7 27.83842933604147 32.22642148237083 21.860187295806245 18.074961885781455 8 25.30679892945712 36.04268731592775 20.115195790197372 18.53531796441776 9 25.0757062655052 17.360820073994613 21.31004243789618 36.25343122260401 10-11 24.283622233844323 27.012717928915908 28.21899112330872 20.48466871393105 12-13 24.606839817004907 24.469610297370256 28.257992789214864 22.665557096409973 14-15 22.044515658884556 26.687852661062472 25.528166943671543 25.739464736381436 16-17 21.735242277142675 29.869150066663643 26.375263129804072 22.020344526389614 18-19 21.746058576676976 28.90694794388981 28.04401993194481 21.302973547488406 20-21 22.791745370538788 28.003676081709916 27.70295745206158 21.501621095689714 22-23 22.52425036484908 27.850103597367376 27.713701150833455 21.911944886950092 24-25 22.30959258766749 28.0101874729182 27.647651609930097 22.03256832948421 26-27 22.037257530533765 28.052832256854337 27.725491490917005 22.184418721694893 28-29 22.02290191261947 28.07491886606727 27.959619706619076 21.94255951469418 30-31 22.315731020836942 28.28117668206961 27.501317824678857 21.901774472414594 32-33 22.03154664389803 28.10630967770116 27.871763316429593 21.99038036197122 34-35 22.305731536662208 27.901246884662186 27.746984718715144 22.046036859960463 36-37 22.472495931832608 27.994981473555363 27.459852046903332 22.072670547708697 38-39 22.235535808600712 28.18340277950982 27.635401951919373 21.945659459970095 40-41 22.19718818407184 27.893680011361916 27.753084045746963 22.15604775881928 42-43 22.1501634503849 27.964625294386448 27.721090940858044 22.16412031437061 44-45 22.23510937129573 28.01116189365793 27.624310498934918 22.129418236111427 46-47 22.22895735157569 28.09282330720913 27.550721856388606 22.127497484826574 48-49 22.150836297629724 27.9796452976064 27.542882331880282 22.326636072883602 50-51 22.1824751671906 27.960909523068295 27.470265793299763 22.386349516441342 52-53 22.194479297202975 27.991942217407377 27.536863334439975 22.27671515094967 54-55 22.29826089614181 28.162030070839787 27.407903845061597 22.1318051879568 56-57 22.174287086732843 27.98835177499931 27.452651543298707 22.38470959496914 58-59 22.203128390285936 27.764499326126657 27.625175737561452 22.407196546025958 60-61 22.2455794598751 27.99004267884893 27.444683882515243 22.31969397876073 62-63 22.05897024736312 28.072628153037193 27.491685121200277 22.37671647839941 64-65 22.210373121950095 27.96243858188239 27.427587828530108 22.399600467637406 66-67 22.059487268974852 27.937350586978994 27.427991600906417 22.575170543139738 68-69 22.44418901520639 28.06820325121025 27.320027170867583 22.167580562715774 70-71 22.238456189160587 27.928734069787232 27.38931060155797 22.44349913949421 72-73 22.14642039588067 27.99220368380076 27.47609577057471 22.385280149743856 74-75 22.16266949518068 28.09071750065506 27.36193143653597 22.384681567628288 76-77 22.242994398491273 28.012719628284955 27.201264983963146 22.543020989260626 78-79 22.3526461755485 28.000057923362746 27.37583806157827 22.271457839510482 80-81 22.158339022446174 28.10548826804235 27.36780445692585 22.368368252585626 82-83 22.158648567334353 28.049054091218316 27.5126832833963 22.279614058051028 84-85 22.2342367382025 27.90021662483269 27.331762498627278 22.533784138337534 86-87 22.346853816526668 27.989225657976995 27.27390213855245 22.39001838694388 88-89 22.18015124486922 27.950590215290642 27.398941183821645 22.47031735601849 90-91 22.117819701622157 27.90613401155878 27.418704703121282 22.557341583697777 92-93 22.228757224742527 28.001617768521076 27.300903523331314 22.46872148340508 94-95 22.209265560847278 27.836448564613942 27.425392989949355 22.528892884589425 96-97 22.235025044012392 27.94535710858695 27.33171338005803 22.487904467342627 98-99 22.239219848994818 27.92275696537781 27.27663964974521 22.56138353588216 100 22.263840086765967 27.831215276682837 27.26229180226003 22.64265283429117 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2472.0 1 2068.0 2 1957.0 3 2323.0 4 2303.5 5 2059.0 6 1838.5 7 1767.0 8 1859.5 9 1892.5 10 1741.0 11 1614.0 12 1603.0 13 1659.5 14 1745.5 15 1931.0 16 2330.0 17 2897.0 18 3677.0 19 4896.5 20 6731.0 21 9415.0 22 14035.5 23 21203.0 24 29509.0 25 39456.0 26 53377.5 27 70714.5 28 91922.5 29 113346.5 30 130584.0 31 147783.0 32 165483.0 33 182392.5 34 197885.0 35 211843.5 36 224316.5 37 234802.5 38 244128.5 39 250795.0 40 256056.5 41 260289.0 42 261232.0 43 261226.0 44 259124.5 45 256935.5 46 256075.5 47 252820.5 48 248953.0 49 248278.0 50 246896.0 51 238305.5 52 226709.0 53 217750.5 54 212757.5 55 205339.5 56 188718.5 57 169200.5 58 153840.0 59 141285.5 60 125489.5 61 107171.5 62 91820.5 63 77167.5 64 62997.5 65 51132.5 66 40867.0 67 32198.5 68 25040.5 69 19483.5 70 15126.5 71 11402.5 72 8695.5 73 6623.5 74 4839.0 75 3478.5 76 2505.5 77 1858.5 78 1454.5 79 1289.5 80 878.5 81 387.0 82 256.0 83 202.5 84 157.5 85 111.5 86 75.0 87 64.5 88 55.5 89 46.5 90 39.0 91 29.0 92 26.5 93 28.0 94 24.0 95 19.5 96 22.0 97 22.0 98 21.0 99 28.0 100 48.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0188417590759319 2 0.0029076788697425773 3 0.001654146201453555 4 0.0025070653365780447 5 0.0021452208550100794 6 0.004109519469236176 7 11.59851132011076 8 1.2923017198855899E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.8769051596567697E-4 18-19 0.0 20-21 6.461508599427949E-6 22-23 0.0 24-25 0.0 26-27 2.2615280097997823E-4 28-29 0.0 30-31 0.0 32-33 2.5846034397711797E-5 34-35 7.107659459370745E-5 36-37 3.87690515965677E-5 38-39 5.1692068795423594E-5 40-41 0.0 42-43 3.87690515965677E-5 44-45 2.2615280097997823E-4 46-47 0.0012406096510901663 48-49 8.593806437239174E-4 50-51 4.070750417639608E-4 52-53 2.843063783748298E-4 54-55 4.7169012775824027E-4 56-57 0.001654146201453555 58-59 3.8769051596567697E-4 60-61 0.0013698398230787255 62-63 0.0025199883537769005 64-65 0.0010919949533033234 66-67 5.799501197382159 68-69 5.446676981818994 70-71 0.0031015241277254158 72-73 0.0015184545208655682 74-75 0.003405215031898529 76-77 0.006248278815646827 78-79 5.403294413082436 80-81 0.005149822353744077 82-83 0.005001207655957233 84-85 5.799856580355127 86-87 0.003922135719852765 88-89 0.001931991071228957 90-91 0.003760598004867067 92-93 0.00403198136604304 94-95 0.004775054854977255 96-97 0.0032759848599099705 98-99 0.005808896230885727 100 0.006913814201387906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7738131.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.91285838747213 #Duplication Level Percentage of deduplicated Percentage of total 1 89.4302777852264 75.04350235343999 2 7.3880376747209695 12.399027183203394 3 1.6569953007705422 4.171296360667959 4 0.6232891118915093 2.0920788392242198 5 0.3093403781804565 1.2978817673891863 6 0.17522689348683396 0.8822273699302416 7 0.11228006917820921 0.6595219081280651 8 0.06957268415096768 0.4670434234237182 9 0.05003720870355253 0.3778888687241033 >10 0.1811970361562583 2.36751262854607 >50 0.003135738195376986 0.1710732164382959 >100 6.101193389215245E-4 0.07094608088482515 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.58460343977118E-5 0.0 9 0.0 0.0 0.0 5.16920687954236E-5 0.0 10-11 0.0 0.0 0.0 6.461508599427949E-5 0.0 12-13 0.0 0.0 0.0 8.399961179256335E-5 0.0 14-15 0.0 0.0 0.0 9.04611203919913E-5 0.0 16-17 0.0 0.0 0.0 9.692262899141924E-5 0.0 18-19 0.0 0.0 0.0 1.2276866338913105E-4 0.0 20-21 0.0 0.0 0.0 1.550762063862708E-4 0.0 22-23 0.0 0.0 0.0 2.2615280097997825E-4 0.0 24-25 0.0 0.0 0.0 4.393825847611006E-4 0.0 26-27 0.0 0.0 0.0 8.270731007267776E-4 0.0 28-29 0.0 2.58460343977118E-5 0.0 0.0026556800343648873 0.0 30-31 0.0 2.58460343977118E-5 0.0 0.007915348034299238 0.0 32-33 0.0 2.58460343977118E-5 0.0 0.01929406467789186 0.0 34-35 0.0 2.58460343977118E-5 0.0 0.0380518241420312 1.29230171988559E-5 36-37 0.0 2.58460343977118E-5 0.0 0.06860183680012655 1.29230171988559E-5 38-39 0.0 2.58460343977118E-5 0.0 0.11090533360058133 1.29230171988559E-5 40-41 0.0 2.58460343977118E-5 0.0 0.15377744315778577 1.29230171988559E-5 42-43 0.0 2.58460343977118E-5 0.0 0.19796770046927353 1.29230171988559E-5 44-45 0.0 2.58460343977118E-5 0.0 0.25230898779046257 1.29230171988559E-5 46-47 0.0 3.87690515965677E-5 0.0 0.32005790545546464 1.29230171988559E-5 48-49 0.0 3.87690515965677E-5 0.0 0.39262710853564 1.29230171988559E-5 50-51 0.0 3.87690515965677E-5 0.0 0.46436277700648904 1.29230171988559E-5 52-53 0.0 3.87690515965677E-5 0.0 0.5357882930645657 1.29230171988559E-5 54-55 0.0 3.87690515965677E-5 0.0 0.6142633150046181 1.29230171988559E-5 56-57 0.0 3.87690515965677E-5 0.0 0.7010542985121343 1.29230171988559E-5 58-59 0.0 3.87690515965677E-5 0.0 0.797485852849997 1.29230171988559E-5 60-61 0.0 3.87690515965677E-5 0.0 0.8965730872222246 1.29230171988559E-5 62-63 0.0 3.87690515965677E-5 0.0 0.9963710875403893 1.29230171988559E-5 64-65 0.0 3.87690515965677E-5 0.0 1.1023204440452092 1.29230171988559E-5 66-67 1.29230171988559E-5 3.87690515965677E-5 0.0 1.2190992889626706 1.29230171988559E-5 68-69 1.29230171988559E-5 3.87690515965677E-5 0.0 1.3400458069267631 1.29230171988559E-5 70-71 1.29230171988559E-5 3.87690515965677E-5 0.0 1.4856494417062724 1.29230171988559E-5 72-73 1.29230171988559E-5 3.87690515965677E-5 0.0 1.6405641103775577 1.29230171988559E-5 74-75 1.29230171988559E-5 4.523056019599565E-5 0.0 1.7810308458205217 1.29230171988559E-5 76-77 1.29230171988559E-5 5.16920687954236E-5 0.0 1.9269704790472013 1.938452579828385E-5 78-79 1.29230171988559E-5 5.16920687954236E-5 0.0 2.097870919993471 2.58460343977118E-5 80-81 1.29230171988559E-5 5.8153577394851546E-5 0.0 2.304083505435615 2.58460343977118E-5 82-83 1.29230171988559E-5 6.461508599427949E-5 0.0 2.519723431924324 2.58460343977118E-5 84-85 1.29230171988559E-5 6.461508599427949E-5 0.0 2.7344329011747153 2.58460343977118E-5 86-87 1.29230171988559E-5 6.461508599427949E-5 0.0 2.9887643411567986 2.58460343977118E-5 88 1.29230171988559E-5 6.461508599427949E-5 0.0 3.2002042870558793 2.58460343977118E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7375 0.0 48.026913 1 GTATCAA 12730 0.0 37.031544 1 ATCAACG 13795 0.0 33.313404 3 TCAACGC 13795 0.0 33.23921 4 CAACGCA 15345 0.0 29.981543 5 TATCAAC 15485 0.0 29.909208 2 AACGCAG 16520 0.0 27.84847 6 GTACATG 24670 0.0 24.37863 1 ACGCAGA 19170 0.0 23.678446 7 GTGGTAT 4005 0.0 23.387787 1 TACATGG 25055 0.0 23.142115 2 CGCAGAG 19140 0.0 22.974464 8 ACATGGG 25740 0.0 21.949514 3 TATACCG 570 0.0 20.64972 5 TGGTATC 4470 0.0 19.806425 2 CATGGGG 18795 0.0 19.468304 4 GCAGAGT 21975 0.0 19.28998 9 GAGTACA 19045 0.0 17.737953 1 CAGAGTA 20760 0.0 17.08485 10-11 AGAGTAC 19320 0.0 17.070366 10-11 >>END_MODULE