FastQCFastQC Report
Wed 25 May 2016
SRR2049506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7738131
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271870.3513380685852953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257470.33272892381894287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153130.1978901623660804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146400.058.239271
GTATCAA268100.041.197391
GTGGTAT54700.036.751841
ATCAACG341750.031.8727573
TCAACGC345700.031.53584
TATCAAC350350.031.1980232
TGGTATC64450.030.6624162
CAACGCA360200.030.283695
AACGCAG375550.029.0582226
ACGCAGA420800.025.7546317
CGCAGAG420500.025.6278788
GCAGAGT454350.023.4599519
GTACATG248900.023.0010261
TACATGG249800.021.9627192
GAGTACT320100.021.64145312-13
ACATGGG251900.021.2191183
CAGAGTA445800.020.9580510-11
AGAGTAC428550.020.72144310-11
GTACTTT341850.020.1613514-15
AGTACTT327100.020.158212-13
Produced by FastQC (version 0.11.5)