##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049506_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7738131 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.842208926160595 31.0 31.0 34.0 30.0 34.0 2 32.017675327543564 33.0 31.0 34.0 30.0 34.0 3 31.970182980877425 33.0 31.0 34.0 30.0 34.0 4 35.64163258543956 37.0 35.0 37.0 33.0 37.0 5 35.745501982326225 37.0 35.0 37.0 35.0 37.0 6 35.79487307723273 37.0 35.0 37.0 35.0 37.0 7 35.80979761133535 37.0 35.0 37.0 35.0 37.0 8 35.79952743110707 37.0 35.0 37.0 35.0 37.0 9 37.54269732574959 39.0 37.0 39.0 35.0 39.0 10-11 37.495378470589344 39.0 37.0 39.0 35.0 39.0 12-13 37.47191376057087 39.0 37.0 39.0 34.5 39.0 14-15 38.751258088031854 40.0 38.0 41.0 35.0 41.0 16-17 38.75727439868878 40.0 38.0 41.0 35.0 41.0 18-19 38.830368147037056 40.0 38.0 41.0 35.0 41.0 20-21 38.858417038946484 40.0 38.0 41.0 35.0 41.0 22-23 38.83543035133419 40.0 38.0 41.0 35.0 41.0 24-25 38.793771519246704 40.0 38.0 41.0 35.0 41.0 26-27 38.699562788482126 40.0 38.0 41.0 34.5 41.0 28-29 38.6134602141008 40.0 38.0 41.0 34.0 41.0 30-31 38.49582263469047 40.0 38.0 41.0 34.0 41.0 32-33 38.350802732597835 40.0 38.0 41.0 34.0 41.0 34-35 38.248035669078234 40.0 38.0 41.0 34.0 41.0 36-37 38.11127796880151 40.0 38.0 41.0 33.5 41.0 38-39 38.01676567636294 40.0 37.5 41.0 33.0 41.0 40-41 37.890489383030605 40.0 37.0 41.0 33.0 41.0 42-43 37.7426699031071 40.0 37.0 41.0 33.0 41.0 44-45 37.54903716414209 40.0 37.0 41.0 33.0 41.0 46-47 37.29354943719614 40.0 37.0 41.0 32.0 41.0 48-49 37.05408502389014 40.0 37.0 41.0 31.5 41.0 50-51 36.834193037052486 40.0 36.0 41.0 31.0 41.0 52-53 36.719233752956626 40.0 36.0 41.0 31.0 41.0 54-55 36.5786284181542 39.0 35.5 41.0 31.0 41.0 56-57 36.39309434022246 39.0 35.0 41.0 31.0 41.0 58-59 36.20603896470608 39.0 35.0 41.0 30.0 41.0 60-61 36.00634365068258 39.0 35.0 41.0 30.0 41.0 62-63 35.79925753389287 38.0 35.0 40.5 30.0 41.0 64-65 35.51845077319058 38.0 35.0 40.0 29.0 41.0 66-67 35.25694893250063 37.0 35.0 40.0 29.0 41.0 68-69 34.947633414321885 37.0 34.0 40.0 29.0 41.0 70-71 34.65905035725035 36.0 34.0 39.0 29.0 41.0 72-73 34.34488631686385 36.0 34.0 39.0 29.0 41.0 74-75 34.01520173282153 35.0 34.0 39.0 28.0 40.5 76-77 33.734889083681836 35.0 34.0 37.5 28.0 39.5 78-79 33.421867114940284 35.0 34.0 37.0 28.0 39.0 80-81 33.13010247564948 35.0 34.0 36.5 27.5 39.0 82-83 32.854321605566 35.0 33.5 36.0 27.0 38.5 84-85 32.59765982250753 35.0 33.0 36.0 27.0 37.0 86-87 32.33804480694369 35.0 33.0 35.5 26.0 37.0 88-89 32.144091706382326 35.0 33.0 35.0 26.0 36.5 90-91 32.004699907509966 35.0 33.0 35.0 26.0 36.0 92-93 31.859279844189768 35.0 33.0 35.0 26.0 36.0 94-95 31.6869893388985 35.0 33.0 35.0 25.5 36.0 96-97 31.529622333868474 35.0 33.0 35.0 25.0 35.5 98-99 31.35407929899352 35.0 33.0 35.0 25.0 35.0 100 31.245145113206277 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3414596101981999 1101 2 0.37820321345378005 1101 3 0.4941187851731783 1101 4 0.3619360957200968 1101 5 0.3039919179129029 1101 6 0.25597917505478307 1101 7 0.27383429730510755 1101 8 0.31941992474988723 1101 9 0.3361020916871382 1101 10-11 0.34336580477645384 1101 12-13 0.30572590310730874 1101 14-15 0.4319192464243713 1101 16-17 0.4317496984134337 1101 18-19 0.44721363570923245 1101 20-21 0.42577718481147997 1101 22-23 0.45809598501429605 1101 24-25 0.45472651439762757 1101 26-27 0.4719270626290353 1101 28-29 0.487756140166546 1101 30-31 0.48116147983802904 1101 32-33 0.5023097980346236 1101 34-35 0.48464505260656665 1101 36-37 0.5016627765539852 1101 38-39 0.5258140944533736 1101 40-41 0.5062797818697149 1101 42-43 0.4729318469017585 1101 44-45 0.481863705202457 1101 46-47 0.4948944999954392 1101 48-49 0.5270259543722275 1101 50-51 0.5578452242259218 1101 52-53 0.5810267762922194 1101 54-55 0.5246119636619966 1101 56-57 0.49831091436293207 1101 58-59 0.5120748297685083 1101 60-61 0.5463224618863975 1101 62-63 0.4929876257896524 1101 64-65 0.4849747904594466 1101 66-67 0.48789491186643374 1101 68-69 0.44749125274714174 1101 70-71 0.4362183291957038 1101 72-73 0.420113369441907 1101 74-75 0.43614630310046465 1101 76-77 0.42981426649395615 1101 78-79 0.4101050337216705 1101 80-81 0.39447713958540476 1101 82-83 0.40740529578496165 1101 84-85 0.44020742361610843 1101 86-87 0.48397075465623374 1101 88-89 0.4744038177044132 1101 90-91 0.4739738736573287 1101 92-93 0.4766780349567554 1101 94-95 0.41941534693546956 1101 96-97 0.4870827855739641 1101 98-99 0.5612191009079659 1101 100 0.6158951767196221 1102 1 0.23513562677048583 1102 2 0.2556244710920694 1102 3 0.3353317534682816 1102 4 0.3072083547340796 1102 5 0.2605866406950028 1102 6 0.19034547700314164 1102 7 0.22484247147409775 1102 8 0.2522443720736902 1102 9 0.3579421995029861 1102 10-11 0.2781035235274487 1102 12-13 0.26259096533338067 1102 14-15 0.35374315786820887 1102 16-17 0.37207666517288374 1102 18-19 0.35169003299623824 1102 20-21 0.36720270549484724 1102 22-23 0.3545704747286038 1102 24-25 0.33899163900971274 1102 26-27 0.3112297588250925 1102 28-29 0.3187857895250872 1102 30-31 0.38344586813504833 1102 32-33 0.41807896574105996 1102 34-35 0.433304952788923 1102 36-37 0.4947726199490745 1102 38-39 0.4697000639961999 1102 40-41 0.4618977234507966 1102 42-43 0.461739653952975 1102 44-45 0.4982908255275049 1102 46-47 0.4433120544632416 1102 48-49 0.4932826128807193 1102 50-51 0.5083426128108783 1102 52-53 0.4804137136182618 1102 54-55 0.4419762740564721 1102 56-57 0.4340990978098347 1102 58-59 0.42274993854414333 1102 60-61 0.39790380766495304 1102 62-63 0.4678604542760709 1102 64-65 0.4678095354765617 1102 66-67 0.4543442272197922 1102 68-69 0.4161768671695114 1102 70-71 0.4486704399228998 1102 72-73 0.44441372616199715 1102 74-75 0.4347064256330171 1102 76-77 0.41037192157921254 1102 78-79 0.3670617754115284 1102 80-81 0.3572008894654317 1102 82-83 0.400955640507739 1102 84-85 0.4061312566775044 1102 86-87 0.4019741219019721 1102 88-89 0.3892050022390805 1102 90-91 0.4178640422595663 1102 92-93 0.434744128151884 1102 94-95 0.4661673744813051 1102 96-97 0.46108170981493046 1102 98-99 0.4765572651591725 1102 100 0.522856217446499 1103 1 0.25177164907417193 1103 2 0.26719576322081906 1103 3 0.36694445898852024 1103 4 0.2995153629162175 1103 5 0.18309555366114694 1103 6 0.2311438544952935 1103 7 0.2431387995640506 1103 8 0.30004348812398973 1103 9 0.26818370028407656 1103 10-11 0.24202055947318968 1103 12-13 0.23363030225509362 1103 14-15 0.3552562353742772 1103 16-17 0.3407138513823398 1103 18-19 0.3609485940838937 1103 20-21 0.3397882777034056 1103 22-23 0.3340611306146428 1103 24-25 0.34073807769005526 1103 26-27 0.3227043327041059 1103 28-29 0.31760245309831703 1103 30-31 0.34122214108448645 1103 32-33 0.3355118864871969 1103 34-35 0.35192261167559025 1103 36-37 0.42841998550055393 1103 38-39 0.38668931816847163 1103 40-41 0.40954999790055524 1103 42-43 0.3952115636091378 1103 44-45 0.42096784747543836 1103 46-47 0.469871146848746 1103 48-49 0.4312776111793326 1103 50-51 0.439942509567409 1103 52-53 0.41625118442394893 1103 54-55 0.38390365948328053 1103 56-57 0.45963725922539567 1103 58-59 0.404366269186184 1103 60-61 0.37879946409233156 1103 62-63 0.3963136969716956 1103 64-65 0.41776347226216615 1103 66-67 0.39684389854208746 1103 68-69 0.3738676355793302 1103 70-71 0.3746560380389994 1103 72-73 0.40128144691740886 1103 74-75 0.38118503064747955 1103 76-77 0.3772945218452506 1103 78-79 0.37740743802579857 1103 80-81 0.338257162956225 1103 82-83 0.3777461123342576 1103 84-85 0.4044599305209502 1103 86-87 0.4307580723760438 1103 88-89 0.42345955152526926 1103 90-91 0.3515115528159214 1103 92-93 0.3790166773761996 1103 94-95 0.4072178187762283 1103 96-97 0.4195571962579443 1103 98-99 0.4524870363994893 1103 100 0.4559326988162731 1104 1 0.2545419840806247 1104 2 0.27156789822954863 1104 3 0.36480659020836725 1104 4 0.2818130173892328 1104 5 0.1954576015366385 1104 6 0.22301624428367006 1104 7 0.16123244939435466 1104 8 0.19466505341131324 1104 9 0.2342182699028612 1104 10-11 0.27999510071239797 1104 12-13 0.27328743617660933 1104 14-15 0.34352994996095276 1104 16-17 0.3174100920323113 1104 18-19 0.34554926263187724 1104 20-21 0.343007819855778 1104 22-23 0.32967733856471426 1104 24-25 0.3108749291820132 1104 26-27 0.3206547526321515 1104 28-29 0.3031820106283689 1104 30-31 0.33091573835495325 1104 32-33 0.34573109020534076 1104 34-35 0.34869133880145853 1104 36-37 0.38338052110335497 1104 38-39 0.3945155632296675 1104 40-41 0.4095652109985579 1104 42-43 0.382230436102887 1104 44-45 0.41179019612214063 1104 46-47 0.44117343488317573 1104 48-49 0.4211978110928598 1104 50-51 0.5056837661582918 1104 52-53 0.47226977063379394 1104 54-55 0.4256256292319378 1104 56-57 0.45798007858264356 1104 58-59 0.4614186790572461 1104 60-61 0.4393376899269654 1104 62-63 0.4177069735253838 1104 64-65 0.3994635579518899 1104 66-67 0.42607453428872333 1104 68-69 0.41308484222837905 1104 70-71 0.3949424967577002 1104 72-73 0.4178948828649709 1104 74-75 0.42678181872700094 1104 76-77 0.44976573422748345 1104 78-79 0.39618903710007913 1104 80-81 0.35777932152567615 1104 82-83 0.3282366069672591 1104 84-85 0.3847436469265233 1104 86-87 0.4110526702379005 1104 88-89 0.3114595412565251 1104 90-91 0.327706447624422 1104 92-93 0.319819302123058 1104 94-95 0.3355209930250034 1104 96-97 0.3575538129704299 1104 98-99 0.47758357190202716 1104 100 0.5012518546435984 1105 1 0.2571396986088672 1105 2 0.2652034106219645 1105 3 0.3312645962529288 1105 4 0.24926731203693464 1105 5 0.18248948820097866 1105 6 0.2069616241998844 1105 7 0.2165537868466032 1105 8 0.2176041294071993 1105 9 0.20555176341026993 1105 10-11 0.19601354850600927 1105 12-13 0.21498529466964555 1105 14-15 0.27675191828417667 1105 16-17 0.246461965273987 1105 18-19 0.25278163978111223 1105 20-21 0.2667793925052564 1105 22-23 0.2711345343434317 1105 24-25 0.2638117773372741 1105 26-27 0.27129746263948107 1105 28-29 0.28549980853895107 1105 30-31 0.3073873770944573 1105 32-33 0.3120778938631332 1105 34-35 0.2538013660610545 1105 36-37 0.2899067993522024 1105 38-39 0.3130217763065204 1105 40-41 0.334004827198946 1105 42-43 0.3273559868512592 1105 44-45 0.34803489111197194 1105 46-47 0.33690383737103957 1105 48-49 0.3006478494910496 1105 50-51 0.3739160736819258 1105 52-53 0.333440494190107 1105 54-55 0.34001733912430865 1105 56-57 0.34018789898745183 1105 58-59 0.33371438612378057 1105 60-61 0.3012256791038652 1105 62-63 0.328594095855415 1105 64-65 0.3502651046069687 1105 66-67 0.3390385736121715 1105 68-69 0.3401950745718665 1105 70-71 0.3528512350468276 1105 72-73 0.36276809845990243 1105 74-75 0.31159636363774723 1105 76-77 0.256341124477764 1105 78-79 0.2577296010906238 1105 80-81 0.255208151291626 1105 82-83 0.23055502111123616 1105 84-85 0.24447959753777582 1105 86-87 0.29824521339443066 1105 88-89 0.22941198838654486 1105 90-91 0.21641869103956424 1105 92-93 0.23486518176246562 1105 94-95 0.2647319185886623 1105 96-97 0.31029498880353046 1105 98-99 0.33857166027272 1105 100 0.3118759265285078 1106 1 0.14517690076508316 1106 2 0.18152786827690193 1106 3 0.29180693421628234 1106 4 0.20672681717835673 1106 5 0.17398450664342846 1106 6 0.1936450652231656 1106 7 0.18324057969982022 1106 8 0.17644181709960094 1106 9 0.18543447862837326 1106 10-11 0.22168970064770122 1106 12-13 0.2158264872078135 1106 14-15 0.2517861972871813 1106 16-17 0.30579307091147 1106 18-19 0.2422713202292357 1106 20-21 0.22587751267869294 1106 22-23 0.24191035457734955 1106 24-25 0.25882331571489203 1106 26-27 0.2642766815377229 1106 28-29 0.2794075233528517 1106 30-31 0.2818985961774203 1106 32-33 0.32039878228686547 1106 34-35 0.25679126823579423 1106 36-37 0.26863175672877304 1106 38-39 0.2808861210749072 1106 40-41 0.2828349160329111 1106 42-43 0.2973547022987262 1106 44-45 0.2635338616551621 1106 46-47 0.25349053511945385 1106 48-49 0.21442234699738805 1106 50-51 0.22508190552573382 1106 52-53 0.23226663209971576 1106 54-55 0.17666048617069663 1106 56-57 0.21333924763777645 1106 58-59 0.19035297267872409 1106 60-61 0.2064063351199934 1106 62-63 0.19871583941468174 1106 64-65 0.15466271223476014 1106 66-67 0.18424094852227313 1106 68-69 0.23545782015126093 1106 70-71 0.23127389004303467 1106 72-73 0.2577116641536321 1106 74-75 0.22165807896985967 1106 76-77 0.19889306324468947 1106 78-79 0.18336186052857784 1106 80-81 0.15594166464307335 1106 82-83 0.1967382898876977 1106 84-85 0.17995920596931825 1106 86-87 0.24194733883249597 1106 88-89 0.24236357821541077 1106 90-91 0.2351307795066333 1106 92-93 0.247411242345855 1106 94-95 0.20248127854680575 1106 96-97 0.21086319169259227 1106 98-99 0.25776107247832414 1106 100 0.2917526809293669 1107 1 0.19731765205715135 1107 2 0.2070884877993251 1107 3 0.302498995377654 1107 4 0.1967371360842023 1107 5 0.19880013980391453 1107 6 0.22032168669412044 1107 7 0.18102873242052198 1107 8 0.18610037765558474 1107 9 0.20029492415988415 1107 10-11 0.20358018536640543 1107 12-13 0.14491651307491082 1107 14-15 0.251088514854942 1107 16-17 0.2305617953290522 1107 18-19 0.2553187156303949 1107 20-21 0.25338006759271536 1107 22-23 0.25060891090908655 1107 24-25 0.2466443485349643 1107 26-27 0.2763362597882306 1107 28-29 0.2813703646140766 1107 30-31 0.27022025470295574 1107 32-33 0.28092520720849024 1107 34-35 0.2497743676506019 1107 36-37 0.29243839853226206 1107 38-39 0.3604176702659885 1107 40-41 0.34776158427809634 1107 42-43 0.3081138685485314 1107 44-45 0.3596559521289322 1107 46-47 0.3776124920800328 1107 48-49 0.3358465650983433 1107 50-51 0.43837906386361425 1107 52-53 0.3381862153355826 1107 54-55 0.3293580730889971 1107 56-57 0.3285750565076455 1107 58-59 0.3088822209486821 1107 60-61 0.28108136611393064 1107 62-63 0.3087591899430393 1107 64-65 0.2929255297457516 1107 66-67 0.30246360989670507 1107 68-69 0.2741835791350411 1107 70-71 0.3431434113216838 1107 72-73 0.35883463449305 1107 74-75 0.29817422044790476 1107 76-77 0.2935469580873118 1107 78-79 0.2586555609889132 1107 80-81 0.2921977730670591 1107 82-83 0.29758997529179254 1107 84-85 0.28779911826378424 1107 86-87 0.35592333121345376 1107 88-89 0.3174403371528314 1107 90-91 0.3015820916703902 1107 92-93 0.26596108772396576 1107 94-95 0.30902516283521564 1107 96-97 0.31209716631025586 1107 98-99 0.3953143205890335 1107 100 0.35483703988744963 1108 1 0.06817175404492914 1108 2 0.09316751452264782 1108 3 0.19163268732519612 1108 4 0.12505335487117009 1108 5 0.09338690457350651 1108 6 0.12984623281509045 1108 7 0.10706743460740142 1108 8 0.10905941302939937 1108 9 0.08029929412134607 1108 10-11 0.09758793296889934 1108 12-13 0.1278512212484415 1108 14-15 0.12183028009634711 1108 16-17 0.1359278131367816 1108 18-19 0.12083713229968396 1108 20-21 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-0.4593494482800935 2215 30-31 -0.4736838457140067 2215 32-33 -0.5469346126410883 2215 34-35 -0.6975595775547632 2215 36-37 -0.6548776655433599 2215 38-39 -0.6087957777743185 2215 40-41 -0.5814859449005567 2215 42-43 -0.49556525155053777 2215 44-45 -0.631258052532516 2215 46-47 -0.6191773133102743 2215 48-49 -0.5742615725947005 2215 50-51 -0.5463104439199427 2215 52-53 -0.6754172737657456 2215 54-55 -0.6198443952661989 2215 56-57 -0.5438624574310893 2215 58-59 -0.514974349988421 2215 60-61 -0.6927921195749533 2215 62-63 -0.6849761551549207 2215 64-65 -0.5375127684651844 2215 66-67 -0.4995609895678754 2215 68-69 -0.49252012260708256 2215 70-71 -0.5456427765519791 2215 72-73 -0.3713645322381467 2215 74-75 -0.5240383062702492 2215 76-77 -0.5014021545831966 2215 78-79 -0.4693949459390723 2215 80-81 -0.4952501131192264 2215 82-83 -0.5047721285356062 2215 84-85 -0.4188988222341905 2215 86-87 -0.37953942012482145 2215 88-89 -0.3466780933439537 2215 90-91 -0.3777540399337198 2215 92-93 -0.5416001796970349 2215 94-95 -0.5839871872240217 2215 96-97 -0.6113936337088752 2215 98-99 -0.5287305183439983 2215 100 -0.632387493763023 2216 1 -0.6092796269423459 2216 2 -0.9159421103809429 2216 3 -0.6275406940476849 2216 4 -1.0869139481898031 2216 5 -0.5339587458921713 2216 6 -0.7279498315043043 2216 7 -0.4765468126331527 2216 8 -0.5293486437238641 2216 9 -0.5736647694305361 2216 10-11 -0.7805425299473256 2216 12-13 -0.6185350469557918 2216 14-15 -0.8994919164694721 2216 16-17 -0.8023403106316565 2216 18-19 -0.8031833057853248 2216 20-21 -0.673144674091759 2216 22-23 -0.6692735874818538 2216 24-25 -0.7346911704954167 2216 26-27 -0.9009458596819968 2216 28-29 -1.1997585571558318 2216 30-31 -1.2794080679795385 2216 32-33 -1.0018945905359118 2216 34-35 -0.9803052252212865 2216 36-37 -0.9405399151145133 2216 38-39 -1.0975656982759077 2216 40-41 -0.9523684977565736 2216 42-43 -1.5293300100786453 2216 44-45 -1.2048659811158657 2216 46-47 -0.9969822598290534 2216 48-49 -0.9428840420739917 2216 50-51 -1.1093037194374347 2216 52-53 -1.0101637698085923 2216 54-55 -1.224722108883519 2216 56-57 -1.271358566495934 2216 58-59 -1.1095771025755283 2216 60-61 -1.3237081142703673 2216 62-63 -1.3167503934846607 2216 64-65 -1.3077386456417983 2216 66-67 -1.201845636933058 2216 68-69 -1.4245581276425199 2216 70-71 -1.2702990038660644 2216 72-73 -1.0666757419242145 2216 74-75 -1.1127241658295688 2216 76-77 -1.2444171164033904 2216 78-79 -1.2973053819139082 2216 80-81 -1.0472667673099778 2216 82-83 -1.166656618616308 2216 84-85 -1.1876577687255931 2216 86-87 -1.3451349401465436 2216 88-89 -1.2226983276876666 2216 90-91 -1.0377433977549622 2216 92-93 -1.0975662415006724 2216 94-95 -1.2602528559464226 2216 96-97 -1.3460324628407356 2216 98-99 -1.3547696939767917 2216 100 -1.3637115323305409 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 445.0 3 64.0 4 89.0 5 163.0 6 442.0 7 1224.0 8 2789.0 9 4714.0 10 6412.0 11 8631.0 12 12165.0 13 17001.0 14 20658.0 15 27525.0 16 35473.0 17 49658.0 18 50674.0 19 27821.0 20 20386.0 21 20190.0 22 21162.0 23 22916.0 24 26732.0 25 33414.0 26 42751.0 27 55268.0 28 73340.0 29 95154.0 30 124305.0 31 160247.0 32 205479.0 33 268487.0 34 364946.0 35 537480.0 36 895735.0 37 1616708.0 38 2093240.0 39 791723.0 40 2520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.42523579297158 20.2183187077618 15.149715395856376 23.206730103410244 2 15.749412938574075 20.24014369387281 39.38782522931613 24.62261813823699 3 18.249344479867872 24.879383003387122 30.451013358524605 26.4202591582204 4 12.921325490543135 17.280209479197637 37.47306531526811 32.32539971499112 5 13.186476613449024 37.31863628885756 35.02514159715987 14.469745500533538 6 30.918812487173668 37.499954768603935 17.084738306650255 14.496494437572144 7 27.0105920925867 32.78777523926643 21.733801094863864 18.467831573283007 8 25.514959620094306 35.59310381279407 19.722100853552362 19.169835713559255 9 25.13246422551607 16.64379506823809 21.295062745803992 36.928677960441846 10-11 24.313299942841496 26.853480510991606 27.848514066251916 20.984705479914982 12-13 25.067135083023935 24.341850033535234 28.33459117383657 22.25642370960426 14-15 22.218699263006553 26.159270939995377 26.385365660443604 25.236664136554467 16-17 21.123343608424307 29.41809850466476 27.23266122013184 22.225896666779096 18-19 21.020017624410855 27.613134230991953 30.243840534620052 21.123007609977137 20-21 22.00515214849684 26.709731329180137 30.236687644600487 21.048428877722543 22-23 21.74183921156155 26.533758603983316 30.356128631060912 21.368273553394225 24-25 21.59650696014322 26.663886925667192 30.240176859244176 21.49942925494541 26-27 21.324367602461113 26.788807271419934 30.292599078511333 21.59422604760762 28-29 21.335770187073862 26.745075031144516 30.523757708590715 21.395397073190907 30-31 21.61783272313923 26.964544256050072 30.1023641233394 21.315258897471303 32-33 21.39438219533545 26.814942810145524 30.393462657868415 21.397212336650608 34-35 21.614650876290412 26.62487233674385 30.257170626860674 21.50330616010507 36-37 21.7875802309369 26.739699806064284 29.900857196653817 21.571862766345 38-39 21.604525692315107 26.894310783831394 30.02492462327143 21.476238900582064 40-41 21.597799261863106 26.69701508025646 30.018941266308364 21.686244391572075 42-43 21.625260673410672 26.83865134875592 29.849281435013182 21.686806542820225 44-45 21.78127379854386 26.902077517167903 29.578699300903537 21.7379493833847 46-47 21.89581049997732 27.05281158977536 29.225610163487797 21.82576774675952 48-49 21.937125385962062 27.08028592434013 28.852257735104253 22.130330954593557 50-51 22.01860500940085 27.188070349287187 28.60213919872899 22.19118544258297 52-53 21.964315414148455 27.35294220271019 28.584544510812755 22.0981978723286 54-55 22.081326873375495 27.529780770059332 28.433358132603338 21.955534223961834 56-57 21.96876739357346 27.28695081538423 28.51488298660232 22.229398804439988 58-59 21.950243980481563 27.028577616860115 28.758810765251862 22.262367637406456 60-61 21.955262840600657 27.215628683463745 28.623565561244696 22.2055429146909 62-63 21.76599233070621 27.2150083786382 28.75052128220626 22.26847800844933 64-65 21.83009695752114 27.16403999880591 28.708450399715385 22.297412643957564 66-67 21.760900661929863 27.15900002209836 28.641993783770264 22.43810553220151 68-69 22.027011432088706 27.267262598681775 28.479486842494655 22.226239126734868 70-71 21.971636303391605 27.17806793397527 28.445299000495083 22.40499676213804 72-73 21.882299485495913 27.233591677370157 28.499898748160245 22.38421008897368 74-75 21.966770787416237 27.337408736037165 28.259427244123934 22.43639323242266 76-77 22.010605661754756 27.29453016497136 28.149885288837833 22.54497888443605 78-79 22.043661107717018 27.272332532859227 28.327924506235203 22.35608185318855 80-81 21.937232193868603 27.3625190243557 28.278692708694773 22.42155607308092 82-83 21.861539413869114 27.329082419575553 28.403202542846106 22.406175623709228 84-85 21.888308688493385 27.215964681910915 28.21160561897957 22.684121010616128 86-87 21.970602462015698 27.273530262023222 28.181430373820245 22.57443690214084 88-89 21.88443380621256 27.203071595610723 28.205187352806778 22.70730724536994 90-91 21.703440418664567 27.20970110600393 28.260530195416774 22.82632827991473 92-93 21.790113118324257 27.263225870149903 28.173405020255636 22.7732559912702 94-95 21.698919377130007 27.119744830874463 28.279767205923793 22.901568586071733 96-97 21.72615066868214 27.232564297502847 28.167563976365866 22.873721057449146 98-99 21.675714074515177 27.210146950270143 28.14480453153528 22.9693344436794 100 21.684463792995828 27.129235581019344 28.1503201001493 23.035980525835527 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 992.0 1 809.0 2 1134.5 3 1653.0 4 1135.5 5 749.5 6 2087.0 7 6103.5 8 10614.5 9 12188.0 10 9398.5 11 5819.0 12 5023.0 13 5417.0 14 6115.5 15 6935.0 16 7747.5 17 8450.5 18 9414.0 19 10819.5 20 12504.5 21 14616.5 22 18460.5 23 24916.5 24 32106.5 25 40680.5 26 53216.0 27 68780.0 28 88929.5 29 109710.0 30 125766.0 31 142210.5 32 159416.5 33 176309.5 34 192430.5 35 205722.0 36 216839.5 37 227847.0 38 238166.5 39 245293.5 40 250990.5 41 254986.5 42 256464.5 43 258951.0 44 258442.5 45 255891.5 46 254143.5 47 249879.5 48 245296.5 49 245226.0 50 245200.5 51 237277.5 52 225134.5 53 216640.0 54 211782.0 55 203921.0 56 188440.5 57 169300.5 58 154070.5 59 143590.0 60 129087.0 61 110441.0 62 95031.0 63 79751.5 64 64754.5 65 52430.0 66 41814.5 67 32694.5 68 25341.5 69 19497.0 70 14939.0 71 11118.0 72 8322.0 73 6269.0 74 4618.5 75 3284.5 76 2188.0 77 1532.5 78 1148.5 79 1005.0 80 667.0 81 224.0 82 110.5 83 72.5 84 38.0 85 27.0 86 21.0 87 14.0 88 8.5 89 5.5 90 3.0 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06148771583215636 2 1.809222407839826E-4 3 1.2923017198855899E-5 4 0.11952498607221822 5 0.002041836717419232 6 0.0018479914594363934 7 0.0 8 0.0 9 5.1692068795423594E-5 10-11 0.0 12-13 1.2923017198855899E-5 14-15 3.230754299713975E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.421531891874149E-4 30-31 2.5199883537769E-4 32-33 1.938452579828385E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.550762063862708E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.0984564619027515E-4 80-81 3.101524127725416E-4 82-83 3.8769051596567697E-4 84-85 0.0 86-87 0.0 88-89 3.87690515965677E-5 90-91 3.87690515965677E-5 92-93 5.0399767075538E-4 94-95 6.138433169456553E-4 96-97 0.0 98-99 3.87690515965677E-5 100 0.0010855334447038955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7738131.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.02448477212881 #Duplication Level Percentage of deduplicated Percentage of total 1 69.55516943481581 34.79461514214782 2 16.304527452479213 16.31251170526605 3 6.05212065158204 9.082626521224562 4 2.7968990952109904 5.596537447902521 5 1.5151352509511729 3.789693014446125 6 0.892695797081352 2.6793988384343685 7 0.5809832447955626 2.034437123749632 8 0.4087867518060298 1.6359477312614987 9 0.30079056803921633 1.3542203871429974 >10 1.4564515462610155 12.935548673987066 >50 0.08558500395611525 2.934146147222457 >100 0.047311958196741215 4.3604129149856385 >500 0.002560608663192865 0.8487711181146211 >1k 8.792007842864476E-4 0.6612628311041967 >5k 2.585884433588112E-5 0.09704402386017917 >10k+ 7.757653300764336E-5 0.882826379150313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27187 0.3513380685852953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25747 0.33272892381894287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15313 0.1978901623660804 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.29230171988559E-5 0.0 9 0.0 0.0 0.0 1.29230171988559E-5 0.0 10-11 0.0 0.0 0.0 1.29230171988559E-5 0.0 12-13 0.0 0.0 0.0 1.938452579828385E-5 0.0 14-15 0.0 0.0 0.0 2.58460343977118E-5 0.0 16-17 0.0 0.0 0.0 3.230754299713975E-5 0.0 18-19 0.0 2.58460343977118E-5 0.0 9.692262899141924E-5 0.0 20-21 0.0 2.58460343977118E-5 0.0 1.29230171988559E-4 0.0 22-23 0.0 2.58460343977118E-5 0.0 2.0030676658226644E-4 0.0 24-25 0.0 5.16920687954236E-5 0.0 4.135365503633888E-4 0.0 26-27 0.0 5.16920687954236E-5 0.0 7.947655577296379E-4 0.0 28-29 0.0 5.16920687954236E-5 0.0 0.0026233724913677474 0.0 30-31 0.0 5.16920687954236E-5 0.0 0.007947655577296379 0.0 32-33 0.0 5.16920687954236E-5 0.0 0.019339295238087854 0.0 34-35 0.0 5.16920687954236E-5 0.0 0.03814874677102262 0.0 36-37 0.0 5.16920687954236E-5 0.0 0.06893137373869737 0.0 38-39 0.0 6.461508599427949E-5 0.0 0.11155794596912355 0.0 40-41 0.0 6.461508599427949E-5 0.0 0.15474020793910054 0.0 42-43 0.0 6.461508599427949E-5 0.0 0.19924061766336082 0.0 44-45 0.0 6.461508599427949E-5 0.0 0.25367236610494187 0.0 46-47 0.0 6.461508599427949E-5 0.0 0.32158928299352907 0.0 48-49 0.0 6.461508599427949E-5 0.0 0.39424894719409637 0.0 50-51 0.0 6.461508599427949E-5 0.0 0.4663593831637123 0.0 52-53 0.0 6.461508599427949E-5 0.0 0.5381919742635528 0.0 54-55 0.0 6.461508599427949E-5 0.0 0.6197685203313307 0.0 56-57 0.0 8.399961179256335E-5 0.0 0.7187652935831663 0.0 58-59 0.0 9.04611203919913E-5 0.0 0.8287918620142254 0.0 60-61 0.0 9.04611203919913E-5 0.0 0.9424110292265665 6.46150859942795E-6 62-63 0.0 9.04611203919913E-5 0.0 1.05656650165266 1.29230171988559E-5 64-65 0.0 9.04611203919913E-5 0.0 1.1783271697002804 1.29230171988559E-5 66-67 0.0 9.04611203919913E-5 0.0 1.3165646846764418 1.29230171988559E-5 68-69 0.0 9.04611203919913E-5 0.0 1.4753562585073836 1.29230171988559E-5 70-71 0.0 9.04611203919913E-5 0.0 1.6468188507018038 1.29230171988559E-5 72-73 0.0 9.04611203919913E-5 0.0 1.8239546474465216 1.29230171988559E-5 74-75 0.0 9.04611203919913E-5 0.0 2.003048281296866 1.29230171988559E-5 76-77 0.0 9.04611203919913E-5 0.0 2.1969129238055025 1.29230171988559E-5 78-79 0.0 9.04611203919913E-5 0.0 2.416390986402272 1.29230171988559E-5 80-81 0.0 9.04611203919913E-5 0.0 2.6534378908808858 1.29230171988559E-5 82-83 0.0 9.04611203919913E-5 0.0 2.900254596361835 2.58460343977118E-5 84-85 0.0 9.04611203919913E-5 0.0 3.147077763351383 2.58460343977118E-5 86-87 0.0 9.04611203919913E-5 0.0 3.411095004724009 2.58460343977118E-5 88 0.0 9.04611203919913E-5 0.0 3.624505710745915 2.58460343977118E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14640 0.0 58.23927 1 GTATCAA 26810 0.0 41.19739 1 GTGGTAT 5470 0.0 36.75184 1 ATCAACG 34175 0.0 31.872757 3 TCAACGC 34570 0.0 31.5358 4 TATCAAC 35035 0.0 31.198023 2 TGGTATC 6445 0.0 30.662416 2 CAACGCA 36020 0.0 30.28369 5 AACGCAG 37555 0.0 29.058222 6 ACGCAGA 42080 0.0 25.754631 7 CGCAGAG 42050 0.0 25.627878 8 GCAGAGT 45435 0.0 23.459951 9 GTACATG 24890 0.0 23.001026 1 TACATGG 24980 0.0 21.962719 2 GAGTACT 32010 0.0 21.641453 12-13 ACATGGG 25190 0.0 21.219118 3 CAGAGTA 44580 0.0 20.95805 10-11 AGAGTAC 42855 0.0 20.721443 10-11 GTACTTT 34185 0.0 20.16135 14-15 AGTACTT 32710 0.0 20.1582 12-13 >>END_MODULE