FastQCFastQC Report
Wed 25 May 2016
SRR2049505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655976
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35050.045.2100141
GTATCAA55450.033.4789051
TCAACGC57550.030.646654
ATCAACG58050.030.3826833
GTCTCGC13050.029.4723641
CAACGCA62600.028.1743565
TATCAAC65100.027.8012052
TCTGTCG13600.027.2536778
AACGCAG67000.026.323696
TCGCTCT14300.025.8146554
CGCTCTG12950.025.3384515
GTACATG86100.025.1346871
TCTCGCT15250.024.542732
CTCGCTC15250.023.8703273
TACATGG85200.023.8300782
GTGGTAT20900.022.8192251
ACATGGG86750.022.6359753
CTGTCGC16800.021.7934469
CGCAGAG81500.021.7409558
ACGCAGA81100.021.556417
Produced by FastQC (version 0.11.5)