FastQCFastQC Report
Wed 25 May 2016
SRR2049505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655976
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145910.399099994091865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145750.39866235445746906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79010.2161119219601004No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58490.1599846388488327No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43670.11944826771291715No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42850.11720536458663842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77800.061.3551031
ATAACGC2650.040.8375933
GTATCAA141550.039.609031
GTGGTAT30350.036.7605441
TGGTATC30150.034.801322
ATCAACG177650.031.1472953
TCAACGC179750.030.8619294
TATCAAC179800.030.8271792
CAACGCA186350.029.8359765
TATAACG3250.028.9550822
AACGCAG194300.028.7115676
CGCAGAG215600.025.6784958
ACGCAGA216100.025.6625777
TAACGCA5300.023.969894
GCAGAGT227500.023.8601749
TCTGTCG12950.022.5006458
CAGAGTA226000.022.10537310-11
GAGTACT185700.021.92954812-13
AGAGTAC215850.020.76068310-11
GTACATG84150.020.2510131
Produced by FastQC (version 0.11.5)