##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049505_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3655976
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58442068547496	31.0	31.0	34.0	30.0	34.0
2	31.8508792180255	33.0	31.0	34.0	30.0	34.0
3	31.824360444379284	33.0	31.0	34.0	30.0	34.0
4	35.51830509828292	37.0	35.0	37.0	33.0	37.0
5	35.66860996899323	37.0	35.0	37.0	33.0	37.0
6	35.76049405138327	37.0	35.0	37.0	33.0	37.0
7	35.74414191996884	37.0	35.0	37.0	33.0	37.0
8	35.731985932073954	37.0	35.0	37.0	33.0	37.0
9	37.450443602474415	39.0	37.0	39.0	34.0	39.0
10-11	37.41007941518215	39.0	37.0	39.0	34.0	39.0
12-13	37.40161724256396	39.0	37.0	39.0	34.0	39.0
14-15	38.63830082582599	40.0	38.0	41.0	34.0	41.0
16-17	38.63695426337591	40.0	38.0	41.0	34.0	41.0
18-19	38.71876292404545	40.0	38.0	41.0	34.0	41.0
20-21	38.74008718875616	40.0	38.0	41.0	34.0	41.0
22-23	38.69228832464984	40.0	38.0	41.0	34.0	41.0
24-25	38.644287462499754	40.0	38.0	41.0	34.0	41.0
26-27	38.531122332312904	40.0	38.0	41.0	34.0	41.0
28-29	38.433597622085045	40.0	38.0	41.0	34.0	41.0
30-31	38.28073707267225	40.0	38.0	41.0	34.0	41.0
32-33	38.078159703455384	40.0	37.0	41.0	33.5	41.0
34-35	37.937860915935985	40.0	37.0	41.0	33.0	41.0
36-37	37.7590369849255	40.0	37.0	41.0	33.0	41.0
38-39	37.631983771228256	40.0	37.0	41.0	33.0	41.0
40-41	37.4860112593737	40.0	37.0	41.0	33.0	41.0
42-43	37.32868678569006	40.0	37.0	41.0	32.5	41.0
44-45	37.117471504189304	40.0	37.0	41.0	31.5	41.0
46-47	36.829050300111376	40.0	36.0	41.0	31.0	41.0
48-49	36.54911301387099	40.0	36.0	41.0	30.0	41.0
50-51	36.32802950566415	40.0	36.0	41.0	30.0	41.0
52-53	36.20639727941321	39.5	35.5	41.0	30.0	41.0
54-55	36.06714103156038	39.0	35.0	41.0	29.5	41.0
56-57	35.88877183548251	39.0	35.0	41.0	29.0	41.0
58-59	35.71807815478001	39.0	35.0	41.0	28.5	41.0
60-61	35.51536415993978	39.0	35.0	41.0	28.0	41.0
62-63	35.28289463606983	38.0	35.0	40.0	28.0	41.0
64-65	34.992370847073396	38.0	34.5	40.0	28.0	41.0
66-67	34.726743146016275	37.0	34.0	40.0	28.0	41.0
68-69	34.399177128077426	37.0	34.0	39.5	27.0	41.0
70-71	34.092008946448225	36.0	34.0	39.0	26.0	41.0
72-73	33.778461483335775	36.0	34.0	39.0	26.0	41.0
74-75	33.43921664146592	35.0	34.0	38.5	26.0	40.0
76-77	33.15885470801778	35.0	34.0	37.5	26.0	39.5
78-79	32.83801069810086	35.0	33.0	37.0	26.0	39.0
80-81	32.53764206876632	35.0	33.0	36.5	26.0	39.0
82-83	32.26099678991328	35.0	33.0	36.0	25.0	38.5
84-85	31.99588550362475	35.0	33.0	36.0	25.0	37.0
86-87	31.740779069665663	35.0	33.0	35.0	24.5	37.0
88-89	31.518961557734514	35.0	33.0	35.0	24.0	36.5
90-91	31.364404881213662	35.0	33.0	35.0	24.0	36.0
92-93	31.203039899605468	35.0	33.0	35.0	23.0	36.0
94-95	31.01069714352611	35.0	32.5	35.0	21.5	36.0
96-97	30.827092409797	35.0	32.0	35.0	20.0	35.0
98-99	30.638642595027974	35.0	32.0	35.0	18.5	35.0
100	30.51866861270424	35.0	32.0	35.0	16.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5655810421930525
1101	2	0.6013388126422647
1101	3	0.6764733026775787
1101	4	0.4854545676746298
1101	5	0.3904635119467841
1101	6	0.3637574476289487
1101	7	0.3940837254546494
1101	8	0.36672128769749435
1101	9	0.41233795151911323
1101	10-11	0.4180743017695363
1101	12-13	0.4381878281404781
1101	14-15	0.6061086508382232
1101	16-17	0.6202922357650067
1101	18-19	0.5755086108548824
1101	20-21	0.5477783039426001
1101	22-23	0.6306060039653119
1101	24-25	0.6063434195900115
1101	26-27	0.6251498270993423
1101	28-29	0.6354263620276726
1101	30-31	0.6636649140270521
1101	32-33	0.6793874679489278
1101	34-35	0.6585668854196527
1101	36-37	0.6923751648241705
1101	38-39	0.6954067310728931
1101	40-41	0.7286192945549814
1101	42-43	0.7026349633455027
1101	44-45	0.6352991248946438
1101	46-47	0.677027028613935
1101	48-49	0.705010604586235
1101	50-51	0.747729061885849
1101	52-53	0.7901592338405763
1101	54-55	0.6835725965881068
1101	56-57	0.6948588523524535
1101	58-59	0.7158336235396092
1101	60-61	0.8072915838077677
1101	62-63	0.7243445211289625
1101	64-65	0.7112509121232264
1101	66-67	0.7226763009645722
1101	68-69	0.6732672963727993
1101	70-71	0.6916411284433508
1101	72-73	0.6522464767121718
1101	74-75	0.7264254402118056
1101	76-77	0.7055553330649218
1101	78-79	0.6356669518268276
1101	80-81	0.6138883467191079
1101	82-83	0.6551782456276456
1101	84-85	0.615472720467757
1101	86-87	0.6966163464991375
1101	88-89	0.6993494170142434
1101	90-91	0.7008831395383375
1101	92-93	0.684976288458536
1101	94-95	0.6531216493992495
1101	96-97	0.7106848591114456
1101	98-99	0.7344569301810182
1101	100	0.759584228549425
1102	1	0.31626086438963696
1102	2	0.4381102330766389
1102	3	0.49179562826184053
1102	4	0.4125790107096208
1102	5	0.3091971890356575
1102	6	0.28918140463759556
1102	7	0.28746378333710965
1102	8	0.31749854487202356
1102	9	0.4512410093524437
1102	10-11	0.3505567646433221
1102	12-13	0.363260415178452
1102	14-15	0.4772678016544276
1102	16-17	0.47755025894309
1102	18-19	0.4862722301102167
1102	20-21	0.4207162120619117
1102	22-23	0.4684318001788057
1102	24-25	0.4724301450154371
1102	26-27	0.43700497972088925
1102	28-29	0.43977507587958087
1102	30-31	0.5085973380523257
1102	32-33	0.4955960881262911
1102	34-35	0.5326253702281107
1102	36-37	0.5480337314771617
1102	38-39	0.5326480982083552
1102	40-41	0.522927537052162
1102	42-43	0.5123809921948492
1102	44-45	0.5471895421294732
1102	46-47	0.6421401061490783
1102	48-49	0.6780731086672915
1102	50-51	0.7532248540602922
1102	52-53	0.7069824430607596
1102	54-55	0.6666055426275079
1102	56-57	0.6596454982517059
1102	58-59	0.6457550417300695
1102	60-61	0.5886428991161665
1102	62-63	0.5958802992911458
1102	64-65	0.6165255454020908
1102	66-67	0.67390207595124
1102	68-69	0.6281102465448356
1102	70-71	0.7045188272337839
1102	72-73	0.6218350548059561
1102	74-75	0.6384815458286823
1102	76-77	0.6263291679980298
1102	78-79	0.5591446020805648
1102	80-81	0.5334811999559932
1102	82-83	0.5432556903767392
1102	84-85	0.47542046800970894
1102	86-87	0.5640631948785249
1102	88-89	0.6217291424564699
1102	90-91	0.5590380919938553
1102	92-93	0.6249018073832744
1102	94-95	0.649501868716218
1102	96-97	0.6741422226413292
1102	98-99	0.6823651622136673
1102	100	0.7619917083908483
1103	1	0.4882713517599484
1103	2	0.4721180425558096
1103	3	0.6086492681616988
1103	4	0.44360435293226175
1103	5	0.339664061974716
1103	6	0.3559878783017112
1103	7	0.3490223682037623
1103	8	0.3024840224833838
1103	9	0.375658628861828
1103	10-11	0.36469985143882866
1103	12-13	0.33343300853673696
1103	14-15	0.46988234356436465
1103	16-17	0.48864322717879105
1103	18-19	0.44501040326460384
1103	20-21	0.47800350313831785
1103	22-23	0.4486277056396375
1103	24-25	0.5164065338049326
1103	26-27	0.5119135299912259
1103	28-29	0.5442382574053752
1103	30-31	0.5541357901371953
1103	32-33	0.4924745589081354
1103	34-35	0.49472842248950144
1103	36-37	0.5757969104260923
1103	38-39	0.4829642223761681
1103	40-41	0.5128682547567891
1103	42-43	0.4958936741057727
1103	44-45	0.49966526199298755
1103	46-47	0.6006936266176339
1103	48-49	0.5687150076091285
1103	50-51	0.6559367092685306
1103	52-53	0.5767249422759093
1103	54-55	0.5502806560182023
1103	56-57	0.6234112449084961
1103	58-59	0.543682485357003
1103	60-61	0.51802956580471
1103	62-63	0.5030271852908967
1103	64-65	0.5193627238379648
1103	66-67	0.5976965481818723
1103	68-69	0.6087525241562588
1103	70-71	0.6652649061068274
1103	72-73	0.6577754120226089
1103	74-75	0.6574069765602104
1103	76-77	0.6408187989790548
1103	78-79	0.5373508517828043
1103	80-81	0.5484061300781278
1103	82-83	0.5960383000649756
1103	84-85	0.6612765917496333
1103	86-87	0.6510016813717421
1103	88-89	0.6600660201781174
1103	90-91	0.6913844613351792
1103	92-93	0.7011963464279063
1103	94-95	0.6363368977579213
1103	96-97	0.6125037173034897
1103	98-99	0.641670810015821
1103	100	0.6242267584439283
1104	1	0.3799403749435264
1104	2	0.371548893386791
1104	3	0.47959551242795584
1104	4	0.3769615783378555
1104	5	0.2724057136373972
1104	6	0.2459580010878213
1104	7	0.19637280969834592
1104	8	0.23055353568393144
1104	9	0.29229586342244573
1104	10-11	0.31775459229839953
1104	12-13	0.28730443351006585
1104	14-15	0.3705368729024485
1104	16-17	0.40938378876025894
1104	18-19	0.4512053690690223
1104	20-21	0.41874810056620504
1104	22-23	0.39630796424556536
1104	24-25	0.3560606473482153
1104	26-27	0.4182878357171731
1104	28-29	0.39685868039896377
1104	30-31	0.4193947318361637
1104	32-33	0.42341472537413694
1104	34-35	0.37218384924810977
1104	36-37	0.42952514533494934
1104	38-39	0.45719309578528566
1104	40-41	0.434753421092239
1104	42-43	0.4547205391519782
1104	44-45	0.525045436257912
1104	46-47	0.5435324932085592
1104	48-49	0.5292275562078288
1104	50-51	0.5070206983890202
1104	52-53	0.5845644797759633
1104	54-55	0.5601173010101093
1104	56-57	0.5153404483425703
1104	58-59	0.533981093827812
1104	60-61	0.5537060519918384
1104	62-63	0.5381091732446635
1104	64-65	0.6051301107436657
1104	66-67	0.568232401293173
1104	68-69	0.46723580319120117
1104	70-71	0.43922363629731365
1104	72-73	0.49527076339519027
1104	74-75	0.5049586583209233
1104	76-77	0.4598307011538765
1104	78-79	0.445036255540856
1104	80-81	0.5101196579833385
1104	82-83	0.5090271008834293
1104	84-85	0.513526910610075
1104	86-87	0.5075327966825149
1104	88-89	0.4629434020191958
1104	90-91	0.4804693347827502
1104	92-93	0.5151684415439846
1104	94-95	0.5500515209401513
1104	96-97	0.5372320965260116
1104	98-99	0.6227508801956461
1104	100	0.615995684089139
1105	1	0.38660737176707016
1105	2	0.40539692391414306
1105	3	0.45897504339666995
1105	4	0.3110005785533261
1105	5	0.2818017176965881
1105	6	0.3128899227398563
1105	7	0.3071300158065924
1105	8	0.24875343350906576
1105	9	0.3314072896306399
1105	10-11	0.3092352416300912
1105	12-13	0.3224698334169318
1105	14-15	0.42151656999219966
1105	16-17	0.4044629308389318
1105	18-19	0.41278401417470434
1105	20-21	0.4042209807852899
1105	22-23	0.4378173719788805
1105	24-25	0.44946550308703337
1105	26-27	0.4433041697942315
1105	28-29	0.5561383041591412
1105	30-31	0.4825491809607314
1105	32-33	0.4831136049259612
1105	34-35	0.5135591959128263
1105	36-37	0.5088027620888482
1105	38-39	0.4472377799120437
1105	40-41	0.4566452113070838
1105	42-43	0.48930295152139536
1105	44-45	0.5075334376619409
1105	46-47	0.5476989861084434
1105	48-49	0.4964051840827963
1105	50-51	0.5187238908322485
1105	52-53	0.4896747869261162
1105	54-55	0.5131416248835805
1105	56-57	0.5992317902391306
1105	58-59	0.5497150238762529
1105	60-61	0.5113710348584277
1105	62-63	0.5535185938367206
1105	64-65	0.5950181875624807
1105	66-67	0.5174953260660615
1105	68-69	0.5479593767397262
1105	70-71	0.5397021031182874
1105	72-73	0.5758557579389176
1105	74-75	0.5987777306148558
1105	76-77	0.5840635208326148
1105	78-79	0.5374078505808555
1105	80-81	0.5306801774764978
1105	82-83	0.5097373725851497
1105	84-85	0.5011518923645681
1105	86-87	0.5368192321003527
1105	88-89	0.5609467783076774
1105	90-91	0.6066546414116054
1105	92-93	0.5856723565094768
1105	94-95	0.5730226200497661
1105	96-97	0.5945377731390877
1105	98-99	0.7037687712230252
1105	100	0.6796938060178128
1106	1	0.2726204895270996
1106	2	0.29532983606555874
1106	3	0.40583902683906814
1106	4	0.25701998942005133
1106	5	0.23654910268988516
1106	6	0.24923377258589596
1106	7	0.2008373436187938
1106	8	0.2436604707810588
1106	9	0.2341009420657656
1106	10-11	0.2660704317557787
1106	12-13	0.24086321196794103
1106	14-15	0.2826395093437455
1106	16-17	0.2729843440577824
1106	18-19	0.2657051852284411
1106	20-21	0.2593778703249754
1106	22-23	0.325633511878479
1106	24-25	0.3124785469809126
1106	26-27	0.3017711863837036
1106	28-29	0.31088791599974286
1106	30-31	0.30005931583163914
1106	32-33	0.3257267360108642
1106	34-35	0.29465087250073196
1106	36-37	0.402116861254882
1106	38-39	0.37578431486521424
1106	40-41	0.40879524143289103
1106	42-43	0.38111429084513304
1106	44-45	0.4756546701259765
1106	46-47	0.4026274880159306
1106	48-49	0.4065678272165769
1106	50-51	0.42235969882659674
1106	52-53	0.4534235232355357
1106	54-55	0.3917590749659894
1106	56-57	0.4029653441570744
1106	58-59	0.37120361155538006
1106	60-61	0.4390637639666224
1106	62-63	0.40657319468430586
1106	64-65	0.4418083772681456
1106	66-67	0.4326396800848187
1106	68-69	0.3795564028304099
1106	70-71	0.41195294244056413
1106	72-73	0.4181870932651819
1106	74-75	0.43712029359661386
1106	76-77	0.4546326378279133
1106	78-79	0.39245647551169327
1106	80-81	0.3303064654270429
1106	82-83	0.29031958996021245
1106	84-85	0.4142144157193073
1106	86-87	0.49445961762374324
1106	88-89	0.39864844377220976
1106	90-91	0.3982151254811406
1106	92-93	0.4484425292114409
1106	94-95	0.4772287182077477
1106	96-97	0.5332256060799168
1106	98-99	0.5510979303944481
1106	100	0.6593427752228678
1107	1	0.2744863974817555
1107	2	0.2504414924068392
1107	3	0.3281052696939497
1107	4	0.2843329514205877
1107	5	0.2462746870276149
1107	6	0.22909230440900785
1107	7	0.2106583667580253
1107	8	0.23455197503417224
1107	9	0.2402633602062636
1107	10-11	0.21659273426890024
1107	12-13	0.1606417833637508
1107	14-15	0.2761837183254343
1107	16-17	0.22499097573140148
1107	18-19	0.2541204847916134
1107	20-21	0.37528326700991244
1107	22-23	0.24681579222722405
1107	24-25	0.2854150313118353
1107	26-27	0.3120138338031353
1107	28-29	0.31428604892747103
1107	30-31	0.34886153241103557
1107	32-33	0.38023102405328046
1107	34-35	0.2973627633991853
1107	36-37	0.37432317701019
1107	38-39	0.329225439356577
1107	40-41	0.32656289434755337
1107	42-43	0.3321578903239413
1107	44-45	0.3037745927729105
1107	46-47	0.2472313323120261
1107	48-49	0.30433866965717016
1107	50-51	0.36587360025542637
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2216	2	-1.5930920730711335
2216	3	-1.4205886104877585
2216	4	-1.8291252291354354
2216	5	-1.1707342793712172
2216	6	-1.2368636441981806
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2216	12-13	-1.1389156956647213
2216	14-15	-1.4829637663364323
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2216	36-37	-1.6742858979852286
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2216	54-55	-2.0799188662106047
2216	56-57	-2.0790864391323396
2216	58-59	-2.0774384588339885
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2216	62-63	-2.117480373341131
2216	64-65	-2.072243474468017
2216	66-67	-2.005708431173403
2216	68-69	-2.2878721179031913
2216	70-71	-2.109133817731859
2216	72-73	-1.9620092797572717
2216	74-75	-1.9692515391519763
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2216	80-81	-1.9837438330289174
2216	82-83	-2.148414579703555
2216	84-85	-2.223038628731217
2216	86-87	-2.431414053414894
2216	88-89	-2.1532517043782917
2216	90-91	-2.1319950862437516
2216	92-93	-2.3495506711764733
2216	94-95	-2.413511248263589
2216	96-97	-2.57731545688776
2216	98-99	-2.648016396917022
2216	100	-2.7964381112204855
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	197.0
3	25.0
4	31.0
5	109.0
6	349.0
7	899.0
8	1929.0
9	3160.0
10	4675.0
11	6978.0
12	9826.0
13	13469.0
14	14761.0
15	17495.0
16	21341.0
17	29253.0
18	29338.0
19	16209.0
20	11984.0
21	11321.0
22	11871.0
23	12994.0
24	14904.0
25	18413.0
26	22625.0
27	28624.0
28	36991.0
29	47865.0
30	61974.0
31	79345.0
32	100414.0
33	132088.0
34	178841.0
35	259313.0
36	412417.0
37	748826.0
38	954678.0
39	339619.0
40	825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75556531065601	20.37549169674616	14.352796674483823	22.516146318114007
2	17.011217269288316	21.129905332921222	38.174492679097476	23.68438471869299
3	18.07063284879332	25.31389702777042	31.006084285017188	25.60938583841907
4	13.139026739977094	18.523690152986866	37.56744145033281	30.769841656703235
5	12.215877541709224	36.32516809786685	34.638882947209254	16.82007141321467
6	29.23493695455115	38.07470266148131	17.87100095351831	14.819359430449236
7	25.540211423707376	32.724968654061186	22.859176318444106	18.875643603787335
8	24.632574174447537	34.06097304796312	21.66190368864566	19.64454908894369
9	24.146684770359542	16.588538874434626	22.88477823705626	36.37999811814957
10-11	23.237214905130667	26.48488392702797	28.875627192301046	21.40227397554032
12-13	24.891933767061623	24.174833775554568	29.01251663201791	21.920715825365896
14-15	22.25775613892327	26.45911145453675	27.340723062931225	23.94240934360875
16-17	20.415082046490458	29.367069149250437	27.69482075374674	22.523028050512366
18-19	20.02254664691453	26.9007099608969	31.603558119637547	21.473185272551024
20-21	21.71284767733705	26.60729993851163	31.104293354223334	20.575559029927987
22-23	21.09695194935634	27.012212333997816	31.136706039645773	20.75412967700007
24-25	20.842997875259574	26.634433595844175	31.472977393724683	21.049591135171568
26-27	20.698987083066193	26.265161478084103	31.624619527042846	21.411231911806862
28-29	20.89736215084857	26.34144253332425	31.5711612592113	21.190034056615875
30-31	21.462518871548742	27.203736686318102	30.77292967536765	20.560814766765507
32-33	20.55882493726024	26.824513296726593	31.539835474804946	21.076826291208228
34-35	20.576475337912502	26.011453576281685	31.626780372737677	21.78529071306814
36-37	21.797202716866853	26.876106407700707	30.428933340919084	20.897757534513357
38-39	21.46480173830463	26.90358197099762	30.744704013374268	20.886912277323482
40-41	20.937719503629125	26.562701724518977	31.020321249373627	21.479257522478264
42-43	20.90827456197743	26.622412182136863	30.74418431630842	21.725128939577285
44-45	21.491976424352895	26.90285713035315	30.36112655006488	21.244039895229072
46-47	21.243998866513348	27.647487292039113	29.593123696654466	21.515390144793074
48-49	21.15524007816244	26.88412068350558	30.045451611279724	21.91518762705226
50-51	21.322951791806073	26.62644667251645	29.795422617653944	22.25517891802353
52-53	21.221104843139013	26.923207373352557	29.560574248846272	22.295113534662153
54-55	21.493275667017507	27.659672820609327	29.21213104243573	21.634920469937438
56-57	20.838703536347065	27.274932877021072	29.268313030501293	22.618050556130566
58-59	20.5943103722037	26.545920732085083	29.909407042210567	22.950361853500652
60-61	21.351343663087505	27.186215117385892	29.18164670665234	22.280794512874262
62-63	21.08866414877997	27.10084803620155	29.29003089735819	22.52045691766029
64-65	20.8404814473618	26.69365170887336	29.775660452913254	22.690206390851582
66-67	20.793298424278497	26.793542955424215	29.246923393370196	23.166235226927093
68-69	21.55357420289411	27.06221266222754	28.885802313800745	22.4984108210776
70-71	21.471174865480517	26.610978846688273	29.127529830611582	22.79031645721963
72-73	20.895678746249978	26.783805473558907	29.594026328400407	22.726489451790712
74-75	20.884300115755682	26.643760790552236	29.31674059129491	23.15519850239717
76-77	20.997717162257082	26.903130655124652	28.793432998466074	23.305719184152192
78-79	21.193499946662584	27.042303410585973	28.830009546030194	22.934187096721253
80-81	20.83193892283721	26.638872823061376	29.066056558794113	23.4631316953073
82-83	20.517961741975103	26.65223819155818	29.14863791092092	23.6811621555458
84-85	20.70570211620645	26.582026249625272	28.89376188465132	23.818509749516956
86-87	21.02698718850824	26.672648654237424	28.70569017762838	23.59467397962596
88-89	20.61523421873818	26.551054048942095	28.835438768907927	23.998272963411797
90-91	20.320735454109677	26.29397513247159	28.77367102733951	24.61161838607922
92-93	20.55596759310609	26.550537267941678	28.464499177436892	24.428995961515344
94-95	20.493327518152952	26.226778085085172	28.72570551800716	24.554188878754722
96-97	20.33879598771983	26.13965463668252	28.876529824046987	24.645019551550668
98-99	20.29097295646538	26.141135245572627	28.762118345705545	24.80577345225645
100	20.46671063180419	26.195490227239222	28.49063999363226	24.847159147324334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1170.0
1	968.0
2	2726.5
3	4399.5
4	2585.0
5	1032.0
6	1685.5
7	3589.5
8	5744.0
9	6775.0
10	5282.0
11	3182.5
12	2705.0
13	2899.0
14	3281.0
15	3742.5
16	4165.5
17	4683.5
18	5296.0
19	5771.0
20	6636.5
21	7688.5
22	9029.5
23	11436.5
24	14380.5
25	18028.0
26	23386.0
27	30560.0
28	40299.5
29	51175.0
30	60339.5
31	69579.5
32	78763.0
33	86968.0
34	94745.5
35	101093.5
36	106586.0
37	111541.0
38	115359.5
39	117666.5
40	119427.0
41	120365.0
42	118723.5
43	118237.0
44	118794.5
45	117738.5
46	117425.5
47	117124.0
48	116735.0
49	117951.5
50	116884.5
51	111711.0
52	105837.0
53	102456.5
54	100742.0
55	98403.0
56	93906.0
57	86962.5
58	79380.5
59	71104.0
60	60845.5
61	49450.0
62	39972.0
63	31675.0
64	24362.5
65	18800.5
66	14486.0
67	11077.0
68	8079.5
69	5786.0
70	4173.0
71	3020.5
72	2205.5
73	1667.5
74	1242.0
75	858.5
76	618.5
77	455.0
78	336.5
79	273.5
80	182.5
81	66.0
82	32.0
83	15.5
84	8.0
85	4.5
86	2.5
87	2.0
88	1.5
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05599052072551899
2	1.6411486289844354E-4
3	0.0
4	0.11734212697238713
5	0.0019146734004818412
6	0.0011488040402891047
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.3676238574870294E-5
14-15	2.7352477149740588E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.3676238574870293E-4
30-31	2.598485329225356E-4
32-33	2.7352477149740588E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.0514357862305442E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.6411486289844354E-4
80-81	2.872010100722762E-4
82-83	3.0087724864714645E-4
84-85	0.0
86-87	1.3676238574870294E-5
88-89	6.838119287435147E-5
90-91	9.573367002409206E-5
92-93	6.97488167318385E-4
94-95	7.521931216178661E-4
96-97	0.0
98-99	1.3676238574870294E-5
100	0.0013402713803372889
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3655976.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.32013673578155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13270420329465	56.11317581648589
2	11.611422564513486	15.865879546090012
3	2.8773675552177314	5.8974643443473065
4	1.162821962020264	3.177766217783768
5	0.6118229996661486	2.0899915497643646
6	0.37788514629108155	1.5490298919016503
7	0.24608375584126158	1.176873309327077
8	0.17274746241327477	0.9441704202267371
9	0.13056725960023857	0.8028335726194125
>10	0.6314971683904014	7.453953360270427
>50	0.030363905635426805	1.4138087448634735
>100	0.013714450268270206	1.6017470883025773
>500	5.608774456555469E-4	0.28482131231138585
>1k	2.8043871037922077E-4	0.4538364437707953
>5k	8.012534582263338E-5	0.3763495025540316
>10k+	8.012534582263338E-5	0.7982988793811553
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14591	0.399099994091865	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14575	0.39866235445746906	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7901	0.2161119219601004	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5849	0.1599846388488327	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4367	0.11944826771291715	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4285	0.11720536458663842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.205743144922177E-5	0.0
5	0.0	0.0	0.0	8.205743144922177E-5	0.0
6	0.0	0.0	0.0	8.205743144922177E-5	0.0
7	0.0	0.0	0.0	1.0940990859896235E-4	2.7352477149740588E-5
8	0.0	0.0	0.0	1.0940990859896235E-4	2.7352477149740588E-5
9	0.0	0.0	0.0	1.3676238574870293E-4	2.7352477149740588E-5
10-11	0.0	0.0	0.0	1.3676238574870293E-4	2.7352477149740588E-5
12-13	0.0	0.0	0.0	1.6411486289844354E-4	2.7352477149740588E-5
14-15	0.0	0.0	0.0	1.6411486289844354E-4	2.7352477149740588E-5
16-17	0.0	0.0	0.0	2.0514357862305442E-4	2.7352477149740588E-5
18-19	0.0	0.0	0.0	2.461722943476653E-4	2.7352477149740588E-5
20-21	0.0	0.0	0.0	3.8293468009636825E-4	2.7352477149740588E-5
22-23	0.0	0.0	0.0	0.0011077753245644938	2.7352477149740588E-5
24-25	0.0	0.0	0.0	0.0025301041363510043	2.7352477149740588E-5
26-27	0.0	0.0	0.0	0.00466359735403077	2.7352477149740588E-5
28-29	0.0	0.0	0.0	0.010968343337045976	2.7352477149740588E-5
30-31	0.0	0.0	0.0	0.02710630485539292	2.7352477149740588E-5
32-33	0.0	0.0	0.0	0.0691470622345442	2.7352477149740588E-5
34-35	0.0	0.0	0.0	0.13787016107326744	2.7352477149740588E-5
36-37	0.0	0.0	0.0	0.24107105735923867	2.7352477149740588E-5
38-39	0.0	0.0	0.0	0.39371155609336606	2.7352477149740588E-5
40-41	0.0	0.0	0.0	0.5841121495327102	2.7352477149740588E-5
42-43	0.0	0.0	0.0	0.789118965770016	2.7352477149740588E-5
44-45	0.0	0.0	0.0	1.0274547754142807	2.7352477149740588E-5
46-47	0.0	0.0	0.0	1.3098554257467774	2.7352477149740588E-5
48-49	0.0	0.0	0.0	1.6270073982980195	2.7352477149740588E-5
50-51	0.0	0.0	0.0	1.9658498852290056	2.7352477149740588E-5
52-53	0.0	0.0	0.0	2.2952967962590565	2.7352477149740588E-5
54-55	0.0	0.0	0.0	2.664924496222076	2.7352477149740588E-5
56-57	0.0	0.0	0.0	3.0975175985837984	2.7352477149740588E-5
58-59	0.0	0.0	0.0	3.5601847495716603	2.7352477149740588E-5
60-61	0.0	0.0	0.0	4.034804933073959	2.7352477149740588E-5
62-63	0.0	0.0	0.0	4.49656945231588	5.4704954299481177E-5
64-65	0.0	0.0	0.0	5.011712330715518	5.4704954299481177E-5
66-67	0.0	0.0	0.0	5.607353549366845	5.4704954299481177E-5
68-69	0.0	0.0	0.0	6.225081893316586	5.4704954299481177E-5
70-71	0.0	0.0	0.0	6.8641314932045505	5.4704954299481177E-5
72-73	0.0	0.0	0.0	7.568416751094646	5.4704954299481177E-5
74-75	0.0	0.0	0.0	8.242710017790053	5.4704954299481177E-5
76-77	0.0	1.3676238574870294E-5	0.0	8.937777490880684	5.4704954299481177E-5
78-79	0.0	4.1028715724610886E-5	0.0	9.690941625437366	5.4704954299481177E-5
80-81	0.0	5.4704954299481177E-5	0.0	10.484095081586968	5.4704954299481177E-5
82-83	0.0	5.4704954299481177E-5	0.0	11.288709225662313	5.4704954299481177E-5
84-85	0.0	5.4704954299481177E-5	0.0	12.054893139342273	5.4704954299481177E-5
86-87	0.0	5.4704954299481177E-5	0.0	12.850043326323805	8.205743144922177E-5
88	0.0	5.4704954299481177E-5	0.0	13.482446274264383	8.205743144922177E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7780	0.0	61.355103	1
ATAACGC	265	0.0	40.837593	3
GTATCAA	14155	0.0	39.60903	1
GTGGTAT	3035	0.0	36.760544	1
TGGTATC	3015	0.0	34.80132	2
ATCAACG	17765	0.0	31.147295	3
TCAACGC	17975	0.0	30.861929	4
TATCAAC	17980	0.0	30.827179	2
CAACGCA	18635	0.0	29.835976	5
TATAACG	325	0.0	28.955082	2
AACGCAG	19430	0.0	28.711567	6
CGCAGAG	21560	0.0	25.678495	8
ACGCAGA	21610	0.0	25.662577	7
TAACGCA	530	0.0	23.96989	4
GCAGAGT	22750	0.0	23.860174	9
TCTGTCG	1295	0.0	22.500645	8
CAGAGTA	22600	0.0	22.105373	10-11
GAGTACT	18570	0.0	21.929548	12-13
AGAGTAC	21585	0.0	20.760683	10-11
GTACATG	8415	0.0	20.251013	1
>>END_MODULE