FastQCFastQC Report
Wed 25 May 2016
SRR2049504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6762846
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83550.036.3396071
GTATCAA123800.032.0932041
TCAACGC129600.029.427314
ATCAACG131250.029.1743763
CAACGCA141950.027.0113145
TATCAAC144650.026.8258632
AACGCAG150750.025.4678546
GTACATG182900.024.7183231
TACATGG181450.024.009082
ACATGGG187650.022.1516863
ACGCAGA173150.021.9063267
CGCAGAG176550.020.9313748
TCTGTCG18150.019.6876498
GTCTCGC16750.019.5630761
CATGGGT53750.018.6671034
CATGGGG118250.018.2255344
CTGTCGC21750.017.6767759
GCAGAGT196450.017.6598389
GAGTACA139300.017.164761
TACCTGG40450.017.0851252
Produced by FastQC (version 0.11.5)