FastQCFastQC Report
Wed 25 May 2016
SRR2049504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6762846
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245930.3636486769031854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241110.3565214999720532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135970.2010544081589319No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73020.10797229450441427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155350.054.640761
GTATCAA271850.038.9313281
ATCAACG339450.030.8983293
TCAACGC341200.030.8088044
TATCAAC346000.030.3680252
CAACGCA354000.029.726075
AACGCAG370150.028.479466
GTGGTAT65850.028.2405761
TGGTATC69450.025.9495872
CGCAGAG418400.025.0598538
ACGCAGA420400.025.0300667
ATAACGC5700.023.1144683
GCAGAGT449050.022.983089
GTACATG190000.022.622961
TACATGG191050.021.8218022
GAGTACT325450.021.72593912-13
TATAACG6600.021.3885442
CAGAGTA438800.020.89590610-11
ACATGGG194450.020.5931283
AGAGTAC406950.020.57961510-11
Produced by FastQC (version 0.11.5)