##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6762846 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75391484590955 31.0 31.0 34.0 30.0 34.0 2 31.836336506849335 33.0 31.0 34.0 30.0 34.0 3 31.856870465481546 33.0 31.0 34.0 30.0 34.0 4 35.53271063691233 37.0 35.0 37.0 33.0 37.0 5 35.67351023518797 37.0 35.0 37.0 33.0 37.0 6 35.74687564968949 37.0 35.0 37.0 33.0 37.0 7 35.73474185276435 37.0 35.0 37.0 33.0 37.0 8 35.71487832193724 37.0 35.0 37.0 33.0 37.0 9 37.41898647403771 39.0 37.0 39.0 34.0 39.0 10-11 37.395571405884446 39.0 37.0 39.0 34.0 39.0 12-13 37.387040308177944 39.0 37.0 39.0 34.0 39.0 14-15 38.62392482395725 40.0 38.0 41.0 34.0 41.0 16-17 38.6282437896708 40.0 38.0 41.0 34.0 41.0 18-19 38.71868019765643 40.0 38.0 41.0 34.0 41.0 20-21 38.753896584367 40.0 38.0 41.0 34.0 41.0 22-23 38.719502114937995 40.0 38.0 41.0 34.0 41.0 24-25 38.677329189515774 40.0 38.0 41.0 34.0 41.0 26-27 38.57037155659023 40.0 38.0 41.0 34.0 41.0 28-29 38.47515128985637 40.0 38.0 41.0 34.0 41.0 30-31 38.33403333448669 40.0 38.0 41.0 34.0 41.0 32-33 38.15308636334466 40.0 37.5 41.0 34.0 41.0 34-35 38.0287393059076 40.0 37.0 41.0 33.0 41.0 36-37 37.86642746263925 40.0 37.0 41.0 33.0 41.0 38-39 37.75413694175499 40.0 37.0 41.0 33.0 41.0 40-41 37.612075596575764 40.0 37.0 41.0 33.0 41.0 42-43 37.44852137694693 40.0 37.0 41.0 33.0 41.0 44-45 37.233400109953706 40.0 37.0 41.0 32.0 41.0 46-47 36.94789346082995 40.0 37.0 41.0 31.0 41.0 48-49 36.6752954303558 40.0 36.0 41.0 30.5 41.0 50-51 36.44896793450568 40.0 36.0 41.0 30.0 41.0 52-53 36.33658455330789 39.5 36.0 41.0 30.0 41.0 54-55 36.19974445669767 39.0 35.0 41.0 30.0 41.0 56-57 36.02683182494471 39.0 35.0 41.0 29.5 41.0 58-59 35.853429088877675 39.0 35.0 41.0 29.0 41.0 60-61 35.65996127961512 39.0 35.0 41.0 29.0 41.0 62-63 35.45692848839083 38.5 35.0 40.5 28.0 41.0 64-65 35.17786638938695 38.0 35.0 40.0 28.0 41.0 66-67 34.92330780561912 37.0 34.5 40.0 28.0 41.0 68-69 34.61010519831444 37.0 34.0 40.0 28.0 41.0 70-71 34.31389758098884 36.5 34.0 39.0 27.5 41.0 72-73 33.994926174572065 36.0 34.0 39.0 27.0 41.0 74-75 33.6635653835678 35.5 34.0 39.0 26.0 40.5 76-77 33.37252659605143 35.0 34.0 37.5 26.0 40.0 78-79 33.04530392973609 35.0 33.5 37.0 26.0 39.0 80-81 32.74048314866256 35.0 33.0 37.0 26.0 39.0 82-83 32.44935172263275 35.0 33.0 36.0 26.0 39.0 84-85 32.17529535642243 35.0 33.0 36.0 25.5 37.0 86-87 31.90058978128439 35.0 33.0 35.5 25.0 37.0 88-89 31.68659385412591 35.0 33.0 35.0 25.0 36.5 90-91 31.52933609607553 35.0 33.0 35.0 25.0 36.0 92-93 31.363918607639448 35.0 33.0 35.0 24.0 36.0 94-95 31.1731549114086 35.0 33.0 35.0 23.5 36.0 96-97 30.992091051607563 35.0 33.0 35.0 22.0 35.5 98-99 30.79616621463804 35.0 32.5 35.0 20.0 35.0 100 30.674402167371547 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38051142587883646 1101 2 0.427120261944264 1101 3 0.4666541461271194 1101 4 0.36929902549537985 1101 5 0.279650930742271 1101 6 0.2748007877234002 1101 7 0.27093038112018775 1101 8 0.2890510553153689 1101 9 0.30436553364206986 1101 10-11 0.3462047620705633 1101 12-13 0.3046474692674437 1101 14-15 0.45859237328615876 1101 16-17 0.46918575780245675 1101 18-19 0.4533983357053444 1101 20-21 0.38604120675066156 1101 22-23 0.4232989291710183 1101 24-25 0.45395261217574046 1101 26-27 0.4169013391258787 1101 28-29 0.40067971023079707 1101 30-31 0.41031263762549486 1101 32-33 0.4873086768391488 1101 34-35 0.5493419130178339 1101 36-37 0.5905735810351302 1101 38-39 0.610702561981519 1101 40-41 0.6574453118650823 1101 42-43 0.576418226483888 1101 44-45 0.6111936467636951 1101 46-47 0.6677461816167636 1101 48-49 0.688128394280433 1101 50-51 0.7406974599470146 1101 52-53 0.738358170936543 1101 54-55 0.6566423660944878 1101 56-57 0.6545980601216499 1101 58-59 0.6438400199208303 1101 60-61 0.6770929731855375 1101 62-63 0.6755931382262546 1101 64-65 0.6553813597852098 1101 66-67 0.638877553987669 1101 68-69 0.6070945548562463 1101 70-71 0.59501826527665 1101 72-73 0.6280017972867569 1101 74-75 0.6167634101494457 1101 76-77 0.5862593844304698 1101 78-79 0.5587059646460446 1101 80-81 0.5833013995059346 1101 82-83 0.5576853889306506 1101 84-85 0.5732950878555201 1101 86-87 0.6151077269173655 1101 88-89 0.6001016473257259 1101 90-91 0.6164647890195916 1101 92-93 0.6220842482557529 1101 94-95 0.5369984585345371 1101 96-97 0.5883277627658359 1101 98-99 0.6306701115708542 1101 100 0.6668638476862192 1102 1 0.3524316219135173 1102 2 0.3652240449984312 1102 3 0.44762673969147215 1102 4 0.33624968752674533 1102 5 0.3138616634985354 1102 6 0.2911326719875902 1102 7 0.317891722409648 1102 8 0.3262710658044483 1102 9 0.366797796366761 1102 10-11 0.3290990277934682 1102 12-13 0.31416533823360737 1102 14-15 0.42090881009521297 1102 16-17 0.43216972182216296 1102 18-19 0.4525314015931059 1102 20-21 0.3932661199180103 1102 22-23 0.4014961608274845 1102 24-25 0.4440415101507895 1102 26-27 0.41046938973791214 1102 28-29 0.4127973853764644 1102 30-31 0.4818337708177083 1102 32-33 0.4634171083251033 1102 34-35 0.48210099001931184 1102 36-37 0.49429128502407593 1102 38-39 0.5081497223504883 1102 40-41 0.5009963542247462 1102 42-43 0.4954405842136751 1102 44-45 0.47941897684547996 1102 46-47 0.488382246107534 1102 48-49 0.5737135183478514 1102 50-51 0.5509782834565939 1102 52-53 0.5596365191086505 1102 54-55 0.4948191572841978 1102 56-57 0.5030690267235585 1102 58-59 0.5047702247318853 1102 60-61 0.5538543517629719 1102 62-63 0.5180433249887528 1102 64-65 0.4910765592243749 1102 66-67 0.5551016664936625 1102 68-69 0.4874562153914539 1102 70-71 0.4678250185886128 1102 72-73 0.49395364191521196 1102 74-75 0.4826364734585269 1102 76-77 0.4525708304918936 1102 78-79 0.4384133027136201 1102 80-81 0.41677523628580815 1102 82-83 0.4295058503846292 1102 84-85 0.45353908298024237 1102 86-87 0.4768188001216771 1102 88-89 0.46414471889950093 1102 90-91 0.45871701217646077 1102 92-93 0.4871865971831326 1102 94-95 0.5157533967292096 1102 96-97 0.582294055522631 1102 98-99 0.6406554159880073 1102 100 0.6872377190712307 1103 1 0.3588686149993734 1103 2 0.3983565144073715 1103 3 0.47122599988476566 1103 4 0.39804978499866195 1103 5 0.29928837604748537 1103 6 0.2586684632635965 1103 7 0.29252216287270016 1103 8 0.33228953776450965 1103 9 0.39244134525571894 1103 10-11 0.3499340748361348 1103 12-13 0.33771065150182267 1103 14-15 0.4853678968159869 1103 16-17 0.4646472641717381 1103 18-19 0.4639523986259704 1103 20-21 0.4460045844516429 1103 22-23 0.3737194843362701 1103 24-25 0.39501166302870416 1103 26-27 0.4282881525665587 1103 28-29 0.40926486637201265 1103 30-31 0.44550437396603826 1103 32-33 0.44808306823439636 1103 34-35 0.4389090146056205 1103 36-37 0.4848807215263662 1103 38-39 0.5129390618106271 1103 40-41 0.5663747036400864 1103 42-43 0.5144884473094606 1103 44-45 0.5292249140371226 1103 46-47 0.5935058233527428 1103 48-49 0.5314446467013525 1103 50-51 0.5590827533262583 1103 52-53 0.5652747893430998 1103 54-55 0.5355617854331314 1103 56-57 0.5640162502780726 1103 58-59 0.45312650350501116 1103 60-61 0.4525771763083526 1103 62-63 0.46357802701177064 1103 64-65 0.5145392222792466 1103 66-67 0.5451002923029193 1103 68-69 0.501680674353544 1103 70-71 0.542917526659707 1103 72-73 0.5324394062016395 1103 74-75 0.4847637830444711 1103 76-77 0.5045912884208121 1103 78-79 0.46578258375937054 1103 80-81 0.44357352979661613 1103 82-83 0.44671533313880474 1103 84-85 0.46441505060820987 1103 86-87 0.5245040945401627 1103 88-89 0.5834501970963935 1103 90-91 0.5505545071911335 1103 92-93 0.5503728974791571 1103 94-95 0.521267807144433 1103 96-97 0.5308388691827943 1103 98-99 0.544090264304856 1103 100 0.5514356988066957 1104 1 0.26744616472429783 1104 2 0.30108506685953174 1104 3 0.3631602466050339 1104 4 0.27815599414898884 1104 5 0.24204666941279385 1104 6 0.2536283776850041 1104 7 0.16038963360200142 1104 8 0.22181394764837137 1104 9 0.24912438117991798 1104 10-11 0.28397030173696436 1104 12-13 0.29472445222594246 1104 14-15 0.38615168155291 1104 16-17 0.34474979979029996 1104 18-19 0.3724270894615387 1104 20-21 0.3644617304995137 1104 22-23 0.3681889822673341 1104 24-25 0.3410834820440769 1104 26-27 0.3485409209400956 1104 28-29 0.3576958274854789 1104 30-31 0.40622807441743447 1104 32-33 0.38584278693003426 1104 34-35 0.40286064741701466 1104 36-37 0.42055791506564333 1104 38-39 0.4291487411259922 1104 40-41 0.4531318420470072 1104 42-43 0.45267980598723057 1104 44-45 0.49618516936227053 1104 46-47 0.5365987754616413 1104 48-49 0.5159445467812915 1104 50-51 0.5058128692335302 1104 52-53 0.48580050112661155 1104 54-55 0.42481288455383037 1104 56-57 0.46597152429415445 1104 58-59 0.47967732167400357 1104 60-61 0.43886718841632444 1104 62-63 0.46592871998679897 1104 64-65 0.4788659226836316 1104 66-67 0.4885547199372837 1104 68-69 0.437375133492111 1104 70-71 0.4839808524696423 1104 72-73 0.48176230082711413 1104 74-75 0.49717076922023296 1104 76-77 0.4550353449403062 1104 78-79 0.46763992141865884 1104 80-81 0.440589519945668 1104 82-83 0.4400203215010592 1104 84-85 0.45561363585766657 1104 86-87 0.49092911628747515 1104 88-89 0.41831025023035906 1104 90-91 0.4251030301374783 1104 92-93 0.4521250251887565 1104 94-95 0.48437416029720737 1104 96-97 0.4777838866981945 1104 98-99 0.5756984336517306 1104 100 0.5980689804167021 1105 1 0.2630626144634789 1105 2 0.28628370150964244 1105 3 0.3793575669332512 1105 4 0.31854561510230184 1105 5 0.20964962950445454 1105 6 0.24878882326704144 1105 7 0.25888822857668004 1105 8 0.2545434887592606 1105 9 0.2862367703315556 1105 10-11 0.28094990692543576 1105 12-13 0.2862281014295718 1105 14-15 0.34192890457683234 1105 16-17 0.3285619009146714 1105 18-19 0.3509463531135708 1105 20-21 0.3373869481584748 1105 22-23 0.3021612188265479 1105 24-25 0.340322114564124 1105 26-27 0.2866558462765454 1105 28-29 0.33674365803508977 1105 30-31 0.336642790960461 1105 32-33 0.37948658345942476 1105 34-35 0.2763587449858065 1105 36-37 0.3701043540491611 1105 38-39 0.30125707203081475 1105 40-41 0.3054784420729888 1105 42-43 0.3068773995960896 1105 44-45 0.322698117129967 1105 46-47 0.33660355933585606 1105 48-49 0.34714285314351656 1105 50-51 0.4080390531473057 1105 52-53 0.39526139507616875 1105 54-55 0.36223994144418725 1105 56-57 0.4013691944450031 1105 58-59 0.32305940956153023 1105 60-61 0.3963686689302932 1105 62-63 0.3339360418013797 1105 64-65 0.378197950472817 1105 66-67 0.3990858119011875 1105 68-69 0.36313809559680976 1105 70-71 0.34462693296981683 1105 72-73 0.3165052824301142 1105 74-75 0.35308510071257615 1105 76-77 0.3290695729020072 1105 78-79 0.31308387372386903 1105 80-81 0.3120466880325736 1105 82-83 0.3183884322832924 1105 84-85 0.33557812692951927 1105 86-87 0.3717198424181305 1105 88-89 0.36367590499790126 1105 90-91 0.354871279959287 1105 92-93 0.36816278063593444 1105 94-95 0.38713872755994316 1105 96-97 0.435327354120453 1105 98-99 0.45672314536113134 1105 100 0.4867152230126699 1106 1 0.1866142572814411 1106 2 0.19777891701621897 1106 3 0.3346389358138495 1106 4 0.26348628978925603 1106 5 0.1991133610729392 1106 6 0.22107748347545453 1106 7 0.1720823202689914 1106 8 0.2374805056611038 1106 9 0.21446870179581623 1106 10-11 0.23382939790246127 1106 12-13 0.21996229429730363 1106 14-15 0.2692648675539715 1106 16-17 0.28448364998391895 1106 18-19 0.28202451741059065 1106 20-21 0.26321790846049 1106 22-23 0.2612276125875681 1106 24-25 0.24907266636085268 1106 26-27 0.31488017144958036 1106 28-29 0.30088778912602265 1106 30-31 0.3023539730824112 1106 32-33 0.3120042066583295 1106 34-35 0.30030172420517687 1106 36-37 0.36809505285637556 1106 38-39 0.328035619301545 1106 40-41 0.38076907380953173 1106 42-43 0.41704864591721247 1106 44-45 0.4318509801868444 1106 46-47 0.43466097645171686 1106 48-49 0.44440310838790964 1106 50-51 0.4737766576175275 1106 52-53 0.5258277015457153 1106 54-55 0.4602177720416165 1106 56-57 0.5042972172245967 1106 58-59 0.4727589634072089 1106 60-61 0.462698854696697 1106 62-63 0.4595773898072011 1106 64-65 0.451922921828249 1106 66-67 0.4372634272109721 1106 68-69 0.41297717015311974 1106 70-71 0.4340251489539071 1106 72-73 0.44384484888185227 1106 74-75 0.443263259879231 1106 76-77 0.43761064396176863 1106 78-79 0.44399666813526295 1106 80-81 0.3654514594275824 1106 82-83 0.32224827037030224 1106 84-85 0.3811296727778384 1106 86-87 0.41084599372121744 1106 88-89 0.42827672833781705 1106 90-91 0.37101911205823157 1106 92-93 0.42421960597387454 1106 94-95 0.4382794034496236 1106 96-97 0.48162045933457875 1106 98-99 0.4978525278580719 1106 100 0.5332098550100604 1107 1 0.1996880949652713 1107 2 0.2080133179500443 1107 3 0.2860038131839957 1107 4 0.22749055889276093 1107 5 0.19751792752991548 1107 6 0.17652509867138377 1107 7 0.14800312337284538 1107 8 0.19136260539736583 1107 9 0.17864214804059486 1107 10-11 0.18505310131145336 1107 12-13 0.1307271334569151 1107 14-15 0.18594867260456738 1107 16-17 0.1782244522933567 1107 18-19 0.21200759896697718 1107 20-21 0.26579092290006656 1107 22-23 0.23745844308401587 1107 24-25 0.23558693299175815 1107 26-27 0.21648407707162676 1107 28-29 0.25170057253853884 1107 30-31 0.28287309258875126 1107 32-33 0.2101877440419173 1107 34-35 0.16737265247622446 1107 36-37 0.2937530929386938 1107 38-39 0.27358661793452654 1107 40-41 0.23673782171339752 1107 42-43 0.2622195143739745 1107 44-45 0.21491627995546736 1107 46-47 0.1764417604087214 1107 48-49 0.1755389088023307 1107 50-51 0.24931346517621478 1107 52-53 0.23080994966326784 1107 54-55 0.2346343794465824 1107 56-57 0.26105894114669326 1107 58-59 0.20620798972268517 1107 60-61 0.1711059072451775 1107 62-63 0.20707834078461929 1107 64-65 0.2046596696127736 1107 66-67 0.21939149127042867 1107 68-69 0.23650931293458655 1107 70-71 0.23027840096111163 1107 72-73 0.18215049836797448 1107 74-75 0.1701517365350469 1107 76-77 0.140410531577011 1107 78-79 0.1207567715021014 1107 80-81 0.14605988363766897 1107 82-83 0.17788970204095733 1107 84-85 0.17489669193624735 1107 86-87 0.2103027648536937 1107 88-89 0.14015131957437177 1107 90-91 0.08039030926558155 1107 92-93 0.039609896626643604 1107 94-95 0.07257780880210163 1107 96-97 0.14655666898387665 1107 98-99 0.19445036305450003 1107 100 0.18055618758191727 1108 1 0.1222622836416214 1108 2 0.18772500793683022 1108 3 0.26981067558065064 1108 4 0.19083595071929693 1108 5 0.16419974841534213 1108 6 0.17085886089216018 1108 7 0.10723371804505177 1108 8 0.18018974442708924 1108 9 0.15881618988647972 1108 10-11 0.15310878780989157 1108 12-13 0.1457336148291546 1108 14-15 0.15602455521677427 1108 16-17 0.24391111529300247 1108 18-19 0.19576730857629343 1108 20-21 0.2142128090879254 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-0.7301889455722801 2215 34-35 -0.7707868967830152 2215 36-37 -0.8652821949702059 2215 38-39 -0.8138581992577585 2215 40-41 -0.7558180770315843 2215 42-43 -0.7684640168236712 2215 44-45 -0.9689887712831009 2215 46-47 -0.9362832421231815 2215 48-49 -0.9423240287059116 2215 50-51 -0.8990710553194887 2215 52-53 -0.9798859095845245 2215 54-55 -0.9826797074863123 2215 56-57 -0.9682385995485774 2215 58-59 -0.9169937594417661 2215 60-61 -1.0778211378979705 2215 62-63 -1.0172362072577457 2215 64-65 -0.8918804761029051 2215 66-67 -0.8122079585339321 2215 68-69 -0.7359907908953005 2215 70-71 -0.8237046961034338 2215 72-73 -0.7528789956313204 2215 74-75 -0.8926241114881464 2215 76-77 -0.8204741585989481 2215 78-79 -0.8047263435970322 2215 80-81 -0.7742665027194278 2215 82-83 -0.7638465875851423 2215 84-85 -0.7395924085584795 2215 86-87 -0.7510086820234037 2215 88-89 -0.7146388787494082 2215 90-91 -0.6672151442453931 2215 92-93 -0.8761564083803037 2215 94-95 -0.8876265099219083 2215 96-97 -0.9637783483121254 2215 98-99 -0.9009138169258897 2215 100 -0.9721872198848374 2216 1 -0.7419518852811748 2216 2 -1.1385444360346746 2216 3 -0.851945858765891 2216 4 -1.2589992004888018 2216 5 -0.7276314164247992 2216 6 -0.9315668536009696 2216 7 -0.5968264033132442 2216 8 -0.6043211999180329 2216 9 -0.7109279945529039 2216 10-11 -0.9131900674155133 2216 12-13 -0.7207906299078815 2216 14-15 -1.0177631216393408 2216 16-17 -0.9587058115906686 2216 18-19 -1.0220691464963352 2216 20-21 -0.8805224445734225 2216 22-23 -0.8098172206589567 2216 24-25 -0.9093290649484871 2216 26-27 -1.0327954252961362 2216 28-29 -1.2660527374676604 2216 30-31 -1.4542633468307073 2216 32-33 -1.1820322633777494 2216 34-35 -1.1302428002193068 2216 36-37 -1.0702352972322586 2216 38-39 -1.2507531610181815 2216 40-41 -1.1022973644717737 2216 42-43 -1.6372029281966576 2216 44-45 -1.3410242974030666 2216 46-47 -1.2548721081562917 2216 48-49 -1.1164551019452063 2216 50-51 -1.2933639146294027 2216 52-53 -1.2233811095013536 2216 54-55 -1.3507289789391308 2216 56-57 -1.407360900253451 2216 58-59 -1.2342223242704975 2216 60-61 -1.4278078010584565 2216 62-63 -1.4173396073466904 2216 64-65 -1.3633601804245998 2216 66-67 -1.2751378063201315 2216 68-69 -1.5933245526986042 2216 70-71 -1.3080887401161476 2216 72-73 -1.143358252382292 2216 74-75 -1.2256975718377845 2216 76-77 -1.3995934810012614 2216 78-79 -1.4508461713776413 2216 80-81 -1.2328182735411168 2216 82-83 -1.3200800668657457 2216 84-85 -1.356140557075694 2216 86-87 -1.563355993907436 2216 88-89 -1.3693788560252393 2216 90-91 -1.2077908867475777 2216 92-93 -1.2972853073340929 2216 94-95 -1.376910709718615 2216 96-97 -1.5145058082280016 2216 98-99 -1.586042127495638 2216 100 -1.608063793178804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 415.0 3 50.0 4 70.0 5 169.0 6 482.0 7 1399.0 8 2834.0 9 4889.0 10 7236.0 11 10862.0 12 15761.0 13 21983.0 14 25207.0 15 31225.0 16 39641.0 17 53784.0 18 53809.0 19 29453.0 20 21359.0 21 20247.0 22 20446.0 23 22437.0 24 25809.0 25 31919.0 26 40291.0 27 51407.0 28 66858.0 29 87341.0 30 113696.0 31 144948.0 32 186101.0 33 241966.0 34 326109.0 35 470202.0 36 753560.0 37 1353113.0 38 1798718.0 39 684950.0 40 2100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33193477787113 20.604496119833403 14.438468811828528 22.625100290466936 2 16.31504889368898 21.054643347849925 38.972052553100504 23.658255205360593 3 18.182936592079724 25.544482308188005 31.000498902385182 25.272082197347096 4 12.848425614452042 17.901763766001114 37.782159110373016 31.467651509173834 5 12.390721294904413 37.235629153448436 35.146450691537794 15.22719886010936 6 29.712557761265757 38.23630919374515 17.528076596059293 14.5230564489298 7 25.79405179417068 33.243445141291105 22.559126734513843 18.403376330024372 8 24.863896057961394 35.10303206667725 20.864958332630966 19.168113542730385 9 24.448216689869426 16.641088181083553 22.427144197449447 36.48355093159758 10-11 23.467035919493064 26.96549647884929 28.69036201622808 20.877105585429568 12-13 24.641622666440334 24.65379210284904 29.039410047015725 21.665175183694906 14-15 21.842712372867872 26.671056830216155 27.31218484052424 24.174045956391733 16-17 20.278304429821407 29.752799339213105 27.965009110069932 22.003887120895552 18-19 20.045185118809446 27.64938755074417 31.50503501040834 20.800392320038043 20-21 21.35019043757613 27.029715004600135 31.25656713164842 20.363527426175313 22-23 20.929280365100727 27.06026427335474 31.381455381358673 20.628999980185856 24-25 20.742768650949614 27.016251737803877 31.468437991934167 20.772541619312342 26-27 20.542889783384098 26.865797328521158 31.571005757043707 21.020307131051037 28-29 20.612479598984894 26.90846113040569 31.649030455041988 20.83002881556743 30-31 21.037209428184568 27.423582645665 31.04918814300938 20.49001978314105 32-33 20.506000063582707 27.149328426128356 31.592695086165662 20.75197642412328 34-35 20.644710821450023 26.707550341971416 31.52725198829014 21.12048684828843 36-37 21.245826091559678 27.148673797983868 30.814268135042553 20.7912319754139 38-39 21.05060502634542 27.205003633085834 30.997778154345077 20.74661318622367 40-41 20.77318483963704 26.996836834669903 31.140366052990114 21.089612272702944 42-43 20.810188491649818 27.013619709808562 30.860823978543944 21.315367819997675 44-45 21.081804908761782 27.217890219590984 30.577489122183177 21.122815749464056 46-47 21.08877682561454 27.627237112895962 30.03445590806001 21.24953015342949 48-49 21.055492022145707 27.360345038168845 29.95459308107859 21.629569858606864 50-51 21.118313207191175 27.322151058888522 29.708742443639853 21.850793290280453 52-53 21.030886996391757 27.470202633624957 29.632347091742144 21.866563278241145 54-55 21.104154966710762 27.882395961700148 29.385764513933925 21.627684557655165 56-57 20.78941321449579 27.55581747684333 29.438028013649877 22.216741295011005 58-59 20.684371222692018 27.096637201954714 29.799556709607973 22.419434865745295 60-61 21.001165781388487 27.432703628028793 29.39431121158163 22.17181937900109 62-63 20.763233407946892 27.346837411350194 29.52388683699141 22.36604234371151 64-65 20.64196789339873 27.11699334865824 29.701415646607956 22.53962311133508 66-67 20.576995247267202 27.1471877372337 29.424076786607294 22.8517402288918 68-69 21.055410695437985 27.37856222069821 29.08406460830248 22.481962475561325 70-71 20.962350761794664 27.033404279795814 29.12741174351745 22.876833214892073 72-73 20.648562750061142 27.104151122175484 29.37151016007166 22.87577596769171 74-75 20.69083045806455 27.068115997318287 29.15250472951772 23.08854881509944 76-77 20.718392818644695 27.186365030343733 28.835079195947976 23.260162955063592 78-79 20.834760557236514 27.247860088313523 28.843676932944195 23.073702421505764 80-81 20.619492456297674 26.99355069954848 28.923424251679382 23.46353259247446 82-83 20.39445190598547 26.94328126388565 29.07936281676287 23.582904013366008 84-85 20.494300772189696 26.8404012157012 28.819811954907742 23.845486057201363 86-87 20.66235871702535 26.931768075156526 28.70594717076213 23.699926037055995 88-89 20.427814160405617 26.796895920842758 28.752168281889734 24.023121636861895 90-91 20.22999204846187 26.55763460408844 28.711107792322533 24.50126555512715 92-93 20.348249348864183 26.638900052907093 28.555140934270106 24.457709663958617 94-95 20.236462620341197 26.37612143782108 28.75427753508993 24.633138406747797 96-97 20.17212871622391 26.349025247654613 28.796589483185038 24.68225655293644 98-99 20.198240562698178 26.311992410293655 28.671014738769667 24.8187522882385 100 20.280310718327268 26.228825094291828 28.527435680676337 24.963428506704563 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1309.0 1 1076.0 2 2372.5 3 3642.0 4 2207.0 5 1155.5 6 2294.0 7 5198.5 8 8239.5 9 9386.0 10 7626.0 11 5299.5 12 4929.0 13 5495.0 14 6187.0 15 6994.5 16 7808.0 17 8712.0 18 9873.0 19 11081.0 20 12830.5 21 15266.5 22 18785.0 23 24466.5 24 31342.0 25 39497.0 26 50841.5 27 65374.5 28 84371.0 29 104425.5 30 121028.5 31 136840.5 32 151874.0 33 166561.5 34 180989.5 35 193787.5 36 203960.0 37 212723.0 38 220706.5 39 225840.0 40 229419.0 41 229925.5 42 226958.0 43 225509.5 44 223910.0 45 221816.0 46 220387.0 47 217081.0 48 214704.0 49 212140.0 50 207038.5 51 199489.5 52 188988.5 53 180649.0 54 176683.5 55 171269.0 56 161057.5 57 147149.0 58 131723.0 59 116362.5 60 99663.0 61 82265.0 62 67538.0 63 54362.0 64 43241.0 65 34500.5 66 26755.5 67 20505.5 68 15532.0 69 11777.5 70 8815.5 71 6468.0 72 4868.0 73 3670.0 74 2742.0 75 2010.0 76 1347.0 77 923.0 78 685.5 79 521.5 80 333.0 81 139.0 82 79.5 83 44.5 84 20.0 85 13.0 86 7.5 87 4.0 88 2.0 89 2.0 90 2.0 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05513950783442356 2 1.4786674130979767E-4 3 0.0 4 0.11718439248801465 5 0.0013603740200501388 6 0.0014195207165740578 7 0.0 8 0.0 9 1.4786674130979768E-5 10-11 0.0 12-13 2.9573348261959537E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.2568673011332802E-4 30-31 2.5137346022665604E-4 32-33 1.4786674130979768E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.5526007837528757E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.2568673011332802E-4 80-81 2.883401455541055E-4 82-83 2.809468084886156E-4 84-85 0.0 86-87 0.0 88-89 4.4360022392939305E-5 90-91 5.175335945842919E-5 92-93 7.097603582870289E-4 94-95 6.432203246976199E-4 96-97 0.0 98-99 2.9573348261959537E-5 100 9.61133818513685E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6762846.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.00822396305006 #Duplication Level Percentage of deduplicated Percentage of total 1 77.66825880906546 48.16070787052667 2 13.868591894951207 17.199335045485505 3 3.915827347215027 7.284404974402368 4 1.573605864041853 3.9030601938830456 5 0.8290538487342279 2.5704078364870315 6 0.49710931690646004 1.8494919514112758 7 0.3227022783552791 1.400713660474847 8 0.2407033431433308 1.1940469448229236 9 0.16578677325158775 0.9252129029306265 >10 0.8525392616490389 9.224955505189959 >50 0.04291198313973104 1.8144720785923212 >100 0.02133502435984757 2.375687443647417 >500 0.001001820388662509 0.4490405391631592 >1k 4.5317754936926403E-4 0.5402440230207831 >5k 4.770289958059071E-5 0.18679593690758178 >10k+ 7.155434937088606E-5 0.9214230930545728 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24593 0.3636486769031854 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24111 0.3565214999720532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13597 0.2010544081589319 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7302 0.10797229450441427 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.914669652391907E-5 0.0 0.0 0.0 6 0.0 5.914669652391907E-5 0.0 0.0 0.0 7 0.0 5.914669652391907E-5 0.0 0.0 0.0 8 0.0 5.914669652391907E-5 0.0 0.0 0.0 9 0.0 5.914669652391907E-5 0.0 1.4786674130979768E-5 0.0 10-11 7.393337065489884E-6 5.914669652391907E-5 0.0 3.6966685327449424E-5 0.0 12-13 1.4786674130979768E-5 6.654003358940895E-5 0.0 5.914669652391907E-5 0.0 14-15 1.4786674130979768E-5 1.3308006717881792E-4 0.0 8.872004478587861E-5 0.0 16-17 1.4786674130979768E-5 1.92226763702737E-4 0.0 1.1829339304783815E-4 0.0 18-19 1.4786674130979768E-5 2.4398012316116617E-4 0.0 1.774400895717572E-4 0.0 20-21 1.4786674130979768E-5 2.5876679729214594E-4 0.0 2.8834014555410544E-4 0.0 22-23 1.4786674130979768E-5 2.6616013435763584E-4 0.0 8.28053751334867E-4 0.0 24-25 1.4786674130979768E-5 2.6616013435763584E-4 0.0 0.0018779076146344304 0.0 26-27 1.4786674130979768E-5 2.6616013435763584E-4 0.0 0.004036762037757477 0.0 28-29 2.2180011196469652E-5 2.8834014555410544E-4 0.0 0.011881092664242244 0.0 30-31 2.9573348261959537E-5 2.9573348261959534E-4 0.0 0.03304082334567429 0.0 32-33 2.9573348261959537E-5 2.9573348261959534E-4 0.0 0.08503076959019916 0.0 34-35 2.9573348261959537E-5 2.9573348261959534E-4 0.0 0.17437185468957891 0.0 36-37 2.9573348261959537E-5 3.696668532744942E-4 0.0 0.3181131139168332 0.0 38-39 2.9573348261959537E-5 4.3620688686390313E-4 0.0 0.5192858154688129 0.0 40-41 2.9573348261959537E-5 4.583868980603728E-4 0.0 0.7465569968619721 0.0 42-43 3.6966685327449424E-5 5.323202687152717E-4 0.0 0.9835947765186432 0.0 44-45 4.4360022392939305E-5 5.323202687152717E-4 0.0 1.2702862079071444 0.0 46-47 4.4360022392939305E-5 5.397136057807615E-4 0.0 1.6181870768608364 0.0 48-49 4.4360022392939305E-5 5.545002799117412E-4 0.0 1.9888372439650408 0.0 50-51 4.4360022392939305E-5 6.062536393701705E-4 0.0 2.371464617115339 0.0 52-53 4.4360022392939305E-5 6.210403135011503E-4 0.0 2.744029658519505 0.0 54-55 4.4360022392939305E-5 6.210403135011503E-4 0.0 3.1646366041752243 0.0 56-57 5.914669652391907E-5 6.210403135011503E-4 0.0 3.6599532208777195 0.0 58-59 7.393337065489883E-5 6.210403135011503E-4 0.0 4.192103442840485 0.0 60-61 7.393337065489883E-5 6.284336505666401E-4 0.0 4.731262548341334 0.0 62-63 7.393337065489883E-5 6.3582698763213E-4 0.0 5.2571654004837605 0.0 64-65 7.393337065489883E-5 6.3582698763213E-4 0.0 5.823214072891798 0.0 66-67 7.393337065489883E-5 6.727936729595794E-4 0.0 6.463040855876358 0.0 68-69 7.393337065489883E-5 6.949736841560491E-4 0.0 7.140898964725798 0.0 70-71 7.393337065489883E-5 7.171536953525187E-4 0.0 7.847775921557286 0.0 72-73 7.393337065489883E-5 7.984804030729075E-4 0.0 8.58118017177975 0.0 74-75 7.393337065489883E-5 8.058737401383973E-4 0.0 9.302806836056892 0.0 76-77 7.393337065489883E-5 8.132670772038873E-4 0.0 10.05045065346749 0.0 78-79 7.393337065489883E-5 8.132670772038873E-4 0.0 10.859555281903507 0.0 80-81 7.393337065489883E-5 8.132670772038873E-4 0.0 11.704066601546153 0.0 82-83 7.393337065489883E-5 8.132670772038873E-4 0.0 12.550574122196483 0.0 84-85 7.393337065489883E-5 8.132670772038873E-4 0.0 13.36797407481998 0.0 86-87 7.393337065489883E-5 8.206604142693771E-4 0.0 14.201639368987554 0.0 88 7.393337065489883E-5 8.28053751334867E-4 0.0 14.85853736725633 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15535 0.0 54.64076 1 GTATCAA 27185 0.0 38.931328 1 ATCAACG 33945 0.0 30.898329 3 TCAACGC 34120 0.0 30.808804 4 TATCAAC 34600 0.0 30.368025 2 CAACGCA 35400 0.0 29.72607 5 AACGCAG 37015 0.0 28.47946 6 GTGGTAT 6585 0.0 28.240576 1 TGGTATC 6945 0.0 25.949587 2 CGCAGAG 41840 0.0 25.059853 8 ACGCAGA 42040 0.0 25.030066 7 ATAACGC 570 0.0 23.114468 3 GCAGAGT 44905 0.0 22.98308 9 GTACATG 19000 0.0 22.62296 1 TACATGG 19105 0.0 21.821802 2 GAGTACT 32545 0.0 21.725939 12-13 TATAACG 660 0.0 21.388544 2 CAGAGTA 43880 0.0 20.895906 10-11 ACATGGG 19445 0.0 20.593128 3 AGAGTAC 40695 0.0 20.579615 10-11 >>END_MODULE