FastQCFastQC Report
Wed 25 May 2016
SRR2049503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664086
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70450.047.311761
GTATCAA114550.036.695871
TCAACGC123500.032.9088024
ATCAACG123200.032.864293
CAACGCA134000.030.2911535
TATCAAC137300.029.8996452
AACGCAG142300.028.4878946
GTGGTAT41950.024.7761141
GTACATG196050.024.548791
ACGCAGA168150.023.8646767
TACATGG200150.022.9147222
CGCAGAG173550.022.8581168
ACATGGG205300.021.7163933
TGGTATC46600.020.430982
GCAGAGT197450.018.9458399
GTACCGT4501.8189894E-1218.1990016
CATGGGT64700.018.1962574
CATGGGG126350.017.8252624
GAGTACA151450.017.7822041
TACCTGG39750.017.641912
Produced by FastQC (version 0.11.5)