##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049503_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6664086 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.710831162743098 31.0 31.0 34.0 27.0 34.0 2 30.90720903061575 31.0 31.0 34.0 27.0 34.0 3 31.023864337885197 31.0 31.0 34.0 27.0 34.0 4 34.6012318868634 37.0 35.0 37.0 32.0 37.0 5 34.629113429808676 37.0 35.0 37.0 32.0 37.0 6 34.62007933270969 37.0 35.0 37.0 31.0 37.0 7 30.65683876228488 37.0 33.0 37.0 2.0 37.0 8 32.420387431974916 37.0 33.0 37.0 17.0 37.0 9 35.19772013746521 39.0 34.0 39.0 28.0 39.0 10-11 35.731015401061754 39.0 36.0 39.0 30.0 39.0 12-13 35.88524600372804 39.0 37.0 39.0 30.0 39.0 14-15 37.0658461640501 40.0 37.0 41.0 31.0 41.0 16-17 37.08446694715524 40.0 37.0 41.0 31.0 41.0 18-19 37.09283883791416 40.0 37.0 41.0 31.0 41.0 20-21 37.16439133588612 40.0 37.0 41.0 31.0 41.0 22-23 37.12389763577481 40.0 37.0 41.0 31.0 41.0 24-25 37.10115085549616 40.0 37.0 41.0 30.5 41.0 26-27 37.12325501201515 40.0 37.0 41.0 31.0 41.0 28-29 37.097985680256826 40.0 37.0 41.0 31.0 41.0 30-31 37.011677670426224 40.0 37.0 41.0 30.0 41.0 32-33 36.94591673936981 40.0 37.0 41.0 30.0 41.0 34-35 36.87319543895442 40.0 37.0 41.0 30.0 41.0 36-37 36.692710073069286 40.0 37.0 41.0 30.0 41.0 38-39 36.57025081909207 40.0 36.0 41.0 30.0 41.0 40-41 36.482585308773025 40.0 36.0 41.0 30.0 41.0 42-43 36.36045505715262 40.0 36.0 41.0 30.0 41.0 44-45 36.242378474707564 40.0 36.0 41.0 29.0 41.0 46-47 36.08057616603387 40.0 35.0 41.0 29.0 41.0 48-49 35.99015071234075 39.5 35.0 41.0 29.0 41.0 50-51 35.84753835709803 39.0 35.0 41.0 28.0 41.0 52-53 35.72053887059681 39.0 35.0 41.0 28.0 41.0 54-55 35.59277828947586 39.0 35.0 41.0 28.0 41.0 56-57 35.38566264000795 39.0 35.0 41.0 28.0 41.0 58-59 35.19888833967629 39.0 35.0 40.5 27.0 41.0 60-61 34.96882295636641 38.0 34.0 40.0 27.0 41.0 62-63 34.72077873844965 38.0 34.0 40.0 26.0 41.0 64-65 34.356908734371075 37.5 34.0 40.0 26.0 41.0 66-67 30.88521486667489 36.0 30.0 40.0 6.5 41.0 68-69 31.230777483964044 35.5 30.0 39.0 11.0 41.0 70-71 31.37734837155463 35.0 29.0 39.0 15.0 41.0 72-73 32.589746966050555 35.0 31.5 39.0 22.5 40.5 74-75 32.654947730266386 35.0 33.0 38.5 23.0 40.0 76-77 32.33788009338415 35.0 33.0 37.0 22.5 39.5 78-79 28.97904153697896 35.0 29.0 36.5 6.0 39.0 80-81 30.719330902992546 35.0 29.5 36.0 15.5 39.0 82-83 31.08235360408014 35.0 31.5 36.0 17.5 37.5 84-85 29.235674854736267 34.5 29.5 35.5 8.0 37.0 86-87 28.830901191851364 34.0 27.0 35.0 7.0 37.0 88-89 30.000493015846434 34.0 30.5 35.0 2.0 36.0 90-91 30.087728459686744 35.0 31.0 35.0 2.0 36.0 92-93 29.875296177150176 35.0 31.0 35.0 2.0 36.0 94-95 29.604037522925125 35.0 31.0 35.0 2.0 35.5 96-97 29.346252884491587 35.0 31.0 35.0 2.0 35.0 98-99 29.093852480295123 34.0 31.0 35.0 2.0 35.0 100 28.85055519991789 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4191308676880574 1101 2 0.42592903895349465 1101 3 0.48608552522820503 1101 4 0.4508592467835726 1101 5 0.5260205264807141 1101 6 0.48901261394532725 1101 7 -12.115767811619161 1101 8 -6.479754886962176 1101 9 -2.0737606801453694 1101 10-11 0.02485005405517171 1101 12-13 0.47272029279525896 1101 14-15 0.7144661675709898 1101 16-17 0.7437167284369295 1101 18-19 0.7128619571124943 1101 20-21 0.6933270993773988 1101 22-23 0.695110647626997 1101 24-25 0.7365664989159484 1101 26-27 0.6989313309333411 1101 28-29 0.6382940242768456 1101 30-31 0.6719684520211118 1101 32-33 0.711745221636157 1101 34-35 0.6921773181531563 1101 36-37 0.790446354475435 1101 38-39 0.8926777112525457 1101 40-41 0.8465347124682339 1101 42-43 0.7891937966591627 1101 44-45 0.6498793023397198 1101 46-47 0.6837877117570414 1101 48-49 0.6237832919103212 1101 50-51 0.7070289734441104 1101 52-53 0.7223639446082473 1101 54-55 0.5804618390130258 1101 56-57 0.6138139730628893 1101 58-59 0.5654448933645142 1101 60-61 0.5579628431584212 1101 62-63 0.5646650452702033 1101 64-65 0.46527113916322804 1101 66-67 -10.163273591153 1101 68-69 -8.0368799417972 1101 70-71 -6.52672902754086 1101 72-73 -1.39339578272903 1101 74-75 -0.0980445180129621 1101 76-77 0.1897965780055415 1101 78-79 3.3089912913044444 1101 80-81 1.172524091667647 1101 82-83 0.49423859835846784 1101 84-85 -5.259782870523125 1101 86-87 -5.729004743181822 1101 88-89 -1.1735989838720222 1101 90-91 0.14409258302849537 1101 92-93 0.4969491850602701 1101 94-95 0.5557385833654394 1101 96-97 0.6468680121072197 1101 98-99 0.6514443027234442 1101 100 0.7343685219551865 1102 1 0.2930366517221401 1102 2 0.32299652559466097 1102 3 0.32998876188027637 1102 4 0.3670594483295133 1102 5 0.3776324755760001 1102 6 0.4068841026597454 1102 7 -12.087555359795395 1102 8 -6.514513351038865 1102 9 -2.173307751246469 1102 10-11 -0.13156835913403597 1102 12-13 0.4110277198257819 1102 14-15 0.5844826971744155 1102 16-17 0.590091769425058 1102 18-19 0.5914404615407349 1102 20-21 0.596905700062436 1102 22-23 0.5768258831329689 1102 24-25 0.6336311592937136 1102 26-27 0.6231412858616423 1102 28-29 0.6387608902237574 1102 30-31 0.6335811769052242 1102 32-33 0.6229676769929284 1102 34-35 0.6650091794362609 1102 36-37 0.7892930400543037 1102 38-39 0.7844237359433777 1102 40-41 0.7098155658935212 1102 42-43 0.678655164804006 1102 44-45 0.706578031706691 1102 46-47 0.7542075345202477 1102 48-49 0.6389494894816252 1102 50-51 0.7189094262234477 1102 52-53 0.6638273950363143 1102 54-55 0.6297569226921311 1102 56-57 0.6544681045316025 1102 58-59 0.6192177964406085 1102 60-61 0.5755417509991929 1102 62-63 0.5933610338989581 1102 64-65 0.46454649224691025 1102 66-67 -10.067308062859592 1102 68-69 -6.454554165517731 1102 70-71 -6.000712991989342 1102 72-73 -0.778180527544535 1102 74-75 0.12544901951633136 1102 76-77 0.2406524495607556 1102 78-79 3.35081767842545 1102 80-81 1.28052114032306 1102 82-83 0.6203182382950843 1102 84-85 -5.2472323007419845 1102 86-87 -5.686994420731612 1102 88-89 -1.122074452332786 1102 90-91 0.1596943001842206 1102 92-93 0.4582965279063025 1102 94-95 0.525432297513369 1102 96-97 0.6029643506379188 1102 98-99 0.6485533629386637 1102 100 0.7362242242739789 1103 1 0.25698913040956484 1103 2 0.2676427253395133 1103 3 0.26329248210943845 1103 4 0.2994132195606696 1103 5 0.31763128512339733 1103 6 0.3367681316187543 1103 7 -12.14902065277331 1103 8 -6.571183106372928 1103 9 -2.228066961988013 1103 10-11 -0.19848468159984378 1103 12-13 0.3112756011921789 1103 14-15 0.4832722238370124 1103 16-17 0.49774647569998365 1103 18-19 0.5449898692447661 1103 20-21 0.5220352640030939 1103 22-23 0.5356765194721191 1103 24-25 0.5858052736214461 1103 26-27 0.5577410867781012 1103 28-29 0.5123455380505462 1103 30-31 0.5522156522560309 1103 32-33 0.6088326331943037 1103 34-35 0.5555906493936362 1103 36-37 0.6149418674417433 1103 38-39 0.5961704543282877 1103 40-41 0.6304692861349679 1103 42-43 0.5557047156282309 1103 44-45 0.5312125557212681 1103 46-47 0.5608587393049689 1103 48-49 0.5174598080892423 1103 50-51 0.5130865791546171 1103 52-53 0.5150137655069855 1103 54-55 0.5284805644617947 1103 56-57 0.5189380591994421 1103 58-59 0.4884105891438679 1103 60-61 0.507163169332749 1103 62-63 0.40803652708940774 1103 64-65 0.3023839577965646 1103 66-67 -10.18450674133209 1103 68-69 -8.134976752505729 1103 70-71 -6.645969624902765 1103 72-73 -1.5001757446420463 1103 74-75 -0.26828664709814376 1103 76-77 0.009371992670047291 1103 78-79 3.129846590179959 1103 80-81 1.0070608677572324 1103 82-83 0.344273227925882 1103 84-85 -5.378709087352039 1103 86-87 -5.856535962645232 1103 88-89 -1.3315567569408735 1103 90-91 -0.06969902233747405 1103 92-93 0.3340991645398397 1103 94-95 0.41032414473852796 1103 96-97 0.5148796617329197 1103 98-99 0.5618684333557908 1103 100 0.6110377217095326 1104 1 0.206147612627106 1104 2 0.24926914032780445 1104 3 0.294656386336662 1104 4 0.2735950945734018 1104 5 0.254894548526849 1104 6 0.2946592083701205 1104 7 -12.118527136113759 1104 8 -6.54734430797501 1104 9 -2.2834102190788386 1104 10-11 -0.2320796222512982 1104 12-13 0.3295530279876857 1104 14-15 0.47623297899797734 1104 16-17 0.4765545738309811 1104 18-19 0.47486726332076046 1104 20-21 0.5160346384379295 1104 22-23 0.5229292901828444 1104 24-25 0.6385727510867127 1104 26-27 0.5827660845131177 1104 28-29 0.5856447981924191 1104 30-31 0.5841370870713902 1104 32-33 0.610054003034044 1104 34-35 0.5793014458685164 1104 36-37 0.6441361693654031 1104 38-39 0.660017384137916 1104 40-41 0.6734614045836906 1104 42-43 0.6145297887082677 1104 44-45 0.6240605888366844 1104 46-47 0.5929091751449889 1104 48-49 0.5456565601927039 1104 50-51 0.6090125786314289 1104 52-53 0.5859641696350906 1104 54-55 0.5371405646973244 1104 56-57 0.532536204203609 1104 58-59 0.524006124494619 1104 60-61 0.4356720386553974 1104 62-63 0.41626226887028395 1104 64-65 0.3060853423090748 1104 66-67 -10.186682805909935 1104 68-69 -8.1213850060034 1104 70-71 -6.544533665041818 1104 72-73 -1.523684680075462 1104 74-75 -0.252056230712121 1104 76-77 0.037596974708542064 1104 78-79 3.199939903294883 1104 80-81 1.0902555172832287 1104 82-83 0.4538624840717702 1104 84-85 -5.247466769540026 1104 86-87 -5.7821419315929 1104 88-89 -1.272784604360286 1104 90-91 0.01108619798207755 1104 92-93 0.3644713966404147 1104 94-95 0.4298864906138178 1104 96-97 0.4674591103997372 1104 98-99 0.5078035055255761 1104 100 0.5931123719463791 1105 1 0.1054838018390356 1105 2 0.12061627879802472 1105 3 0.132735420154372 1105 4 0.187056839023235 1105 5 0.1750243188820022 1105 6 0.203563994441474 1105 7 -12.14606704712795 1105 8 -6.609479428116195 1105 9 -2.4080520338397307 1105 10-11 -0.36461979550990264 1105 12-13 0.2304178989818979 1105 14-15 0.3912230358717679 1105 16-17 0.406036404725441 1105 18-19 0.4110330948682446 1105 20-21 0.45152232809419957 1105 22-23 0.417546003351724 1105 24-25 0.46074989312492676 1105 26-27 0.4189786583570836 1105 28-29 0.4081116094998052 1105 30-31 0.42679219356109144 1105 32-33 0.4178617209979336 1105 34-35 0.4683411252950336 1105 36-37 0.5146200250822233 1105 38-39 0.46289444543441505 1105 40-41 0.46576345991505264 1105 42-43 0.4323863616982706 1105 44-45 0.3405915532534465 1105 46-47 0.3297618654402399 1105 48-49 0.34755426788104415 1105 50-51 0.38863197703535945 1105 52-53 0.3780731429001989 1105 54-55 0.38545470247832725 1105 56-57 0.3470237675393335 1105 58-59 0.3841511788974046 1105 60-61 0.3077290838330953 1105 62-63 0.23348077534771505 1105 64-65 0.12100607062460256 1105 66-67 -10.198202538139924 1105 68-69 -8.250324304916969 1105 70-71 -6.653469994314424 1105 72-73 -1.6531578864529521 1105 74-75 -0.4598506702441014 1105 76-77 -0.18044641972404207 1105 78-79 2.949858736802181 1105 80-81 0.8514935214481127 1105 82-83 0.14738842669269303 1105 84-85 -5.433624475204283 1105 86-87 -5.9099700159750235 1105 88-89 -1.5251746297647522 1105 90-91 -0.22910835413031805 1105 92-93 0.11829757956586562 1105 94-95 0.14609648264306685 1105 96-97 0.19064951314067002 1105 98-99 0.25553232116332225 1105 100 0.3701510637087253 1106 1 0.1329609340222362 1106 2 0.14659462987534155 1106 3 0.1633917179077713 1106 4 0.09196469069102164 1106 5 0.10396445653575626 1106 6 0.14387033399345484 1106 7 -12.201017956667695 1106 8 -6.7249212072344875 1106 9 -2.577347782725383 1106 10-11 -0.4769292093817441 1106 12-13 0.18129663040014776 1106 14-15 0.25413942249404187 1106 16-17 0.2889628172619254 1106 18-19 0.33982620060305635 1106 20-21 0.27093911243559177 1106 22-23 0.2542619857734536 1106 24-25 0.3576116849707063 1106 26-27 0.2988959094424217 1106 28-29 0.25916641619114955 1106 30-31 0.2369121973830346 1106 32-33 0.295884464086015 1106 34-35 0.34409969980892186 1106 36-37 0.4254912877315462 1106 38-39 0.4193543687932504 1106 40-41 0.3431395577058254 1106 42-43 0.32313301290243857 1106 44-45 0.26121546915743465 1106 46-47 0.3307551204087602 1106 48-49 0.3322959013664146 1106 50-51 0.35397584047105113 1106 52-53 0.3145460125449304 1106 54-55 0.26549039075042913 1106 56-57 0.2692771158629341 1106 58-59 0.23025707517151517 1106 60-61 0.216361324350828 1106 62-63 0.19140030409472786 1106 64-65 0.08537098875561355 1106 66-67 -10.317225963645004 1106 68-69 -8.249481653134477 1106 70-71 -6.796507032899342 1106 72-73 -1.7236980359755094 1106 74-75 -0.562967440957614 1106 76-77 -0.23365856983185296 1106 78-79 2.8653395411137055 1106 80-81 0.7464782435084949 1106 82-83 0.04284524468230444 1106 84-85 -5.585603996083094 1106 86-87 -6.055146922379375 1106 88-89 -1.6063949978401943 1106 90-91 -0.4068874216962932 1106 92-93 -0.059820418955176535 1106 94-95 0.06017707970697472 1106 96-97 0.15997131063200243 1106 98-99 0.15575530006128346 1106 100 0.22293304534993297 1107 1 0.11544368718382714 1107 2 0.12401024016645934 1107 3 0.12022517263228849 1107 4 0.11327107139673842 1107 5 0.13120742812552066 1107 6 0.12805937005573043 1107 7 -12.286452755003406 1107 8 -6.730648169567164 1107 9 -2.4955606643073125 1107 10-11 -0.4997012915455272 1107 12-13 0.1159212043661384 1107 14-15 0.28448357990586004 1107 16-17 0.30973551344557393 1107 18-19 0.3514292267958581 1107 20-21 0.3163501287604191 1107 22-23 0.3784942152405577 1107 24-25 0.4019044899329671 1107 26-27 0.2944956086383357 1107 28-29 0.3286435630818758 1107 30-31 0.3213359649441472 1107 32-33 0.3267831257513194 1107 34-35 0.33551013413542563 1107 36-37 0.40926912932765447 1107 38-39 0.4374465818072295 1107 40-41 0.3681704757702988 1107 42-43 0.36755861759970543 1107 44-45 0.30897546547667787 1107 46-47 0.3942353497647204 1107 48-49 0.3428318543041229 1107 50-51 0.3935921015247601 1107 52-53 0.33225396220728953 1107 54-55 0.3580565911511684 1107 56-57 0.37398244576952067 1107 58-59 0.3694986755214842 1107 60-61 0.3367979889245163 1107 62-63 0.33208205831758164 1107 64-65 0.18497090450726716 1107 66-67 -10.268877728809958 1107 68-69 -8.254034986597706 1107 70-71 -6.723620802884589 1107 72-73 -1.6566478762809211 1107 74-75 -0.424236644416645 1107 76-77 -0.17352611893708314 1107 78-79 2.921153610454631 1107 80-81 0.8672749882948274 1107 82-83 0.20915666076696482 1107 84-85 -5.497984609665195 1107 86-87 -5.978113238706513 1107 88-89 -1.4879268522296307 1107 90-91 -0.2388110419830909 1107 92-93 0.08446386442041387 1107 94-95 0.15542891573153383 1107 96-97 0.1671410030646463 1107 98-99 0.19671542611549953 1107 100 0.31954119813920556 1108 1 0.0743796049504688 1108 2 0.10715828646061709 1108 3 0.09756255557293869 1108 4 0.026449144558043258 1108 5 0.06182525109326775 1108 6 0.11829758178748762 1108 7 -12.296816007317982 1108 8 -6.721081658933418 1108 9 -2.615306737136194 1108 10-11 -0.5538955151571727 1108 12-13 0.06789127080202917 1108 14-15 0.2463287976126054 1108 16-17 0.18554383659370188 1108 18-19 0.2531553152830526 1108 20-21 0.29757646095387713 1108 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2216 14-15 -1.2933834845025345 2216 16-17 -1.4493032671114605 2216 18-19 -1.9368626024145215 2216 20-21 -1.5738718096676934 2216 22-23 -1.358732996018226 2216 24-25 -1.387881136219029 2216 26-27 -1.8122037315232475 2216 28-29 -1.9911232807017925 2216 30-31 -1.9271213121849797 2216 32-33 -2.8291618984569027 2216 34-35 -1.9521561475914169 2216 36-37 -1.6165625542700042 2216 38-39 -1.814620760520981 2216 40-41 -1.8841252866470128 2216 42-43 -1.4699128323663047 2216 44-45 -1.6516772690238213 2216 46-47 -1.92263918927177 2216 48-49 -1.998317967097094 2216 50-51 -2.4735288083679947 2216 52-53 -2.1496609866831946 2216 54-55 -1.8311709100870956 2216 56-57 -1.8588739185295964 2216 58-59 -2.1738210349379017 2216 60-61 -2.4213447485714354 2216 62-63 -2.272696217369983 2216 64-65 -1.951793781743362 2216 66-67 1.460771458180087 2216 68-69 0.6461929731922176 2216 70-71 0.28528472061006127 2216 72-73 -1.4472484977082303 2216 74-75 -2.205521864397401 2216 76-77 -1.9526964803829152 2216 78-79 0.5520270392242885 2216 80-81 -0.8309335984665296 2216 82-83 -1.6992809555520338 2216 84-85 0.1100429728667649 2216 86-87 0.07793877938830818 2216 88-89 -1.155280256444712 2216 90-91 -1.4299749344869639 2216 92-93 -1.4868098916787105 2216 94-95 -1.4751113906497224 2216 96-97 -1.4554856029448544 2216 98-99 -1.4781662972633534 2216 100 -2.6044344024372705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 101837.0 3 19979.0 4 11639.0 5 10657.0 6 10178.0 7 12018.0 8 14369.0 9 16005.0 10 17606.0 11 19324.0 12 21790.0 13 23885.0 14 26291.0 15 27986.0 16 29741.0 17 30734.0 18 28879.0 19 27162.0 20 26383.0 21 26273.0 22 28511.0 23 32903.0 24 39036.0 25 48357.0 26 60587.0 27 73762.0 28 92009.0 29 118062.0 30 144635.0 31 175239.0 32 231119.0 33 320288.0 34 474256.0 35 649981.0 36 721708.0 37 1189429.0 38 1322983.0 39 437507.0 40 978.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5352977692074 20.435829442043698 14.294455857826746 22.734416930922155 2 15.738529819228656 20.705945237975186 39.73854302473271 23.816981918063448 3 18.360408309528683 25.596746921201856 30.390920479162254 25.65192429010721 4 12.953376506152914 17.523782941409067 38.35779718078637 31.165043371651652 5 13.205354855758603 37.684637585641994 34.99615017137777 14.113857387221628 6 30.272107873731336 37.795333016896834 18.060680474522687 13.871878634849141 7 26.886019001162932 32.79326399893776 22.40217175553012 17.91854524436919 8 24.762705643354543 35.97046916861517 20.911374793182443 18.355450394847843 9 24.67678238246025 17.723855904620677 21.817170426672163 35.782191286246906 10-11 23.576218854318505 27.459572100360045 28.75209743691783 20.212111608403614 12-13 24.147414964332693 25.07608695325961 28.659136451720464 22.11736163068724 14-15 21.752082731225258 27.36890700390121 25.93393902779766 24.945071237075872 16-17 21.171359859629252 30.49678450837788 26.64202855718802 21.689827074804853 18-19 21.13953061230002 29.462307359178734 28.46787991631561 20.930282112205635 20-21 22.31686553241251 28.752939919513338 27.939707556273103 20.99048699180105 22-23 22.02997530343996 28.66402084246812 27.94105598277093 21.36494787132099 24-25 21.751782615050285 28.712939778988446 27.9984231896167 21.536854416344568 26-27 21.50648653365347 28.679176879073477 28.029882014391944 21.784454572881113 28-29 21.542211490067807 28.771357392446617 28.132642645968254 21.553788471517326 30-31 21.858886574993182 29.0622675037507 27.734936193800618 21.343909727455497 32-33 21.453162737270006 28.85802777125442 28.09058557926557 21.598223912210006 34-35 21.64246065154825 28.514406451122554 28.083011602275814 21.760121295053384 36-37 21.925034547138743 28.755479185958517 27.73590905155293 21.58357721534981 38-39 21.71831966154098 28.808924434648652 27.9156811601771 21.55707474363326 40-41 21.549286728892756 28.58830903442723 28.109098532041756 21.753305704638265 42-43 21.522399549225437 28.60670294571573 28.048651840425205 21.82224566463363 44-45 21.61785556268916 28.668406993349187 27.97906145857163 21.73467598539002 46-47 21.53307566586542 28.794770960541733 27.8995449515386 21.77260842205424 48-49 21.429934286863954 28.54091297914531 28.05041475963419 21.978737974356548 50-51 21.39056371041077 28.447342911078234 28.070733156664257 22.09136022184674 52-53 21.35998124267292 28.4879305978898 28.07920844079719 22.072879718640092 54-55 21.404354051577677 28.753282915514088 27.921355884998395 21.921007147909844 56-57 21.2775888596087 28.5300857582278 27.89995058498939 22.292374797174116 58-59 21.221173620001572 28.188566528510073 28.13862697592675 22.451632875561604 60-61 21.444101606486452 28.43361418634859 27.830649053179002 22.291635153985965 62-63 21.261078523454785 28.428990680692273 27.905333912973273 22.40459688287967 64-65 21.280902459291607 28.223844117939457 28.022223847869434 22.473029574899506 66-67 21.26706103337247 28.26190548263188 27.784642640355727 22.68639084363992 68-69 21.776350038585626 28.384703185927478 27.571051644260443 22.26789513122645 70-71 21.550834434605587 28.20948925652737 27.72924376604123 22.510432542825818 72-73 21.408148259303722 28.288512905162065 27.862717587034812 22.4406212484994 74-75 21.457655798497687 28.318352535405882 27.767763567803904 22.45622809829252 76-77 21.571516114724957 28.351127532896243 27.581248180893287 22.496108171485517 78-79 21.718608313466643 28.427478311919618 27.585921292201014 22.26799208241272 80-81 21.58317980258124 28.272486033666265 27.662089681968123 22.482244481784377 82-83 21.603019143366016 28.238875305018073 27.731758022835358 22.426347528780553 84-85 21.763864679069 28.113547467364736 27.564048947197445 22.558538906368817 86-87 21.863982237798222 28.2372998584069 27.490062559523913 22.408655344270965 88-89 21.821752454230797 28.062644213422022 27.549862870400542 22.56574046194664 90-91 21.919643617508584 27.96727237994424 27.445959742650327 22.66712425989685 92-93 22.14866751412815 28.04898179318388 27.256718853426932 22.545631839261038 94-95 22.18705437585326 27.787303184427103 27.36017929824127 22.665463141478362 96-97 22.2140562396348 27.815164972481636 27.324298130045012 22.646480657838552 98-99 22.305247185161125 27.821503758033217 27.23655481444615 22.63669424235951 100 22.525982479506272 27.84847586711437 27.041377628543025 22.58416402483633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3882.0 1 3130.5 2 2846.5 3 3311.0 4 3031.0 5 2626.0 6 2411.5 7 2318.5 8 2306.0 9 2277.5 10 2096.0 11 1896.5 12 1935.5 13 1999.0 14 2067.0 15 2354.5 16 2728.0 17 3269.0 18 4134.0 19 5332.5 20 7138.0 21 9802.0 22 13894.0 23 19685.0 24 27200.5 25 36048.0 26 47359.0 27 62147.0 28 80326.0 29 99214.5 30 116101.0 31 132419.0 32 148458.5 33 163751.5 34 178806.0 35 193063.5 36 204818.5 37 214726.0 38 223847.5 39 230018.5 40 234718.5 41 237106.5 42 235094.5 43 233788.0 44 231575.5 45 227500.0 46 224643.0 47 220786.5 48 215175.5 49 211490.5 50 207681.0 51 199303.0 52 189941.0 53 183012.0 54 176367.0 55 166522.5 56 152281.0 57 136087.5 58 121256.0 59 107763.5 60 93450.5 61 78935.5 62 66143.5 63 54033.0 64 43387.5 65 34925.0 66 27891.0 67 21994.0 68 16966.0 69 12984.0 70 9780.0 71 7335.0 72 5668.0 73 4368.5 74 3218.0 75 2288.0 76 1624.5 77 1157.5 78 794.0 79 582.0 80 407.0 81 258.0 82 177.5 83 140.5 84 115.5 85 86.0 86 69.5 87 54.5 88 40.0 89 31.5 90 21.0 91 21.0 92 21.0 93 16.0 94 16.5 95 15.5 96 16.5 97 19.5 98 17.5 99 21.5 100 39.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.020137795340576334 2 0.002971150132216181 3 0.0018307086673251217 4 0.0025659932960048837 5 0.0022958887385306852 6 0.004426713580827139 7 11.624354787738334 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.926132705970481E-4 18-19 0.0 20-21 7.502904374283285E-6 22-23 0.0 24-25 0.0 26-27 1.57560991859949E-4 28-29 0.0 30-31 0.0 32-33 1.500580874856657E-5 34-35 8.253194811711613E-5 36-37 4.501742624569971E-5 38-39 0.0 40-41 0.0 42-43 1.500580874856657E-5 44-45 1.7256680060851557E-4 46-47 0.0010579095167739432 48-49 6.82764298059779E-4 50-51 3.901510274627308E-4 52-53 3.526365055913144E-4 54-55 6.002323499426629E-4 56-57 0.0017856912410794219 58-59 4.4267135808271377E-4 60-61 0.0015305924923537902 62-63 0.002686039765993416 64-65 0.001102926943019643 66-67 5.812590053609752 68-69 5.469024559406947 70-71 0.0030386762715847305 72-73 0.001290499552376725 74-75 0.0033312895421817785 76-77 0.00667008198873784 78-79 5.427015797815335 80-81 0.005747224750700997 82-83 0.005124483687635483 84-85 5.8126950942709925 86-87 0.00408908288398439 88-89 0.0020182812766822034 90-91 0.003518862151538861 92-93 0.003946527700873008 94-95 0.004929408173904118 96-97 0.003939024796498725 98-99 0.006414983240012208 100 0.0069176778330891885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6664086.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.9926411604189 #Duplication Level Percentage of deduplicated Percentage of total 1 91.83178322163505 80.8052114814271 2 5.8392974603176135 10.276304121093464 3 1.2802997697331902 3.3797087464769873 4 0.45870472780625066 1.6145056204977217 5 0.22788739252187698 1.0026206777580526 6 0.11864186589239178 0.6263766679243065 7 0.07165729885912976 0.4413720489525411 8 0.045860803733720285 0.3228330597015719 9 0.027566654201761243 0.21830964400520375 >10 0.09730205676538238 1.2389800204381316 >50 8.995848186344181E-4 0.05087190481704914 >100 8.136553118803184E-5 0.009828995075687368 >500 1.7798183811783853E-5 0.0130770118321699 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.500580874856657E-5 0.0 10-11 0.0 0.0 0.0 3.751452187141642E-5 0.0 12-13 0.0 0.0 0.0 7.502904374283285E-5 0.0 14-15 0.0 7.502904374283285E-6 0.0 8.253194811711613E-5 0.0 16-17 0.0 1.500580874856657E-5 0.0 1.3505227873709914E-4 0.0 18-19 0.0 3.001161749713314E-5 0.0 1.8006970498279885E-4 0.0 20-21 0.0 3.001161749713314E-5 0.0 3.076190793456147E-4 0.0 22-23 0.0 3.001161749713314E-5 0.0 6.977701068083455E-4 0.0 24-25 0.0 6.002323499426628E-5 0.0 0.0017931941454537052 0.0 26-27 0.0 6.002323499426628E-5 0.0 0.004119094501481523 0.0 28-29 0.0 6.002323499426628E-5 0.0 0.011824577293870457 0.0 30-31 0.0 6.002323499426628E-5 0.0 0.03283270954186365 0.0 32-33 0.0 7.502904374283285E-5 0.0 0.08306465432769025 0.0 34-35 0.0 7.502904374283285E-5 0.0 0.16764489533898572 0.0 36-37 0.0 8.253194811711613E-5 0.0 0.30252460727547636 0.0 38-39 0.0 9.003485249139942E-5 0.0 0.48672841256850524 0.0 40-41 0.0 9.003485249139942E-5 0.0 0.6864107095856806 0.0 42-43 0.0 9.003485249139942E-5 0.0 0.8963794884999984 0.0 44-45 0.0 9.75377568656827E-5 0.0 1.1590411648349077 0.0 46-47 0.0 1.1254356561424928E-4 0.0 1.480428073707332 0.0 48-49 0.0 1.2004646998853256E-4 0.0 1.8155828121065665 0.0 50-51 0.0 1.2004646998853256E-4 0.0 2.159028259839384 0.0 52-53 0.0 1.2004646998853256E-4 0.0 2.4978444155732684 0.0 54-55 0.0 1.2004646998853256E-4 0.0 2.8677901215560544 0.0 56-57 0.0 1.2004646998853256E-4 0.0 3.2604621248885444 0.0 58-59 0.0 1.2004646998853256E-4 0.0 3.688998011130108 0.0 60-61 0.0 1.2004646998853256E-4 0.0 4.12080516367886 0.0 62-63 1.500580874856657E-5 1.2004646998853256E-4 0.0 4.5461298068482305 0.0 64-65 1.500580874856657E-5 1.2004646998853256E-4 0.0 5.001105928104769 0.0 66-67 1.500580874856657E-5 1.2004646998853256E-4 1.500580874856657E-5 5.497655642499211 0.0 68-69 1.500580874856657E-5 1.2004646998853256E-4 1.500580874856657E-5 5.982500826069772 0.0 70-71 2.2508713122849856E-5 1.2004646998853256E-4 1.500580874856657E-5 6.547266947035197 0.0 72-73 3.001161749713314E-5 1.3505227873709914E-4 1.500580874856657E-5 7.137647983534427 0.0 74-75 3.001161749713314E-5 1.3505227873709914E-4 1.500580874856657E-5 7.661410732094394 0.0 76-77 4.501742624569971E-5 1.500580874856657E-4 1.500580874856657E-5 8.190980728640056 0.0 78-79 5.2520330619982996E-5 1.57560991859949E-4 1.500580874856657E-5 8.791813310932662 0.0 80-81 6.002323499426628E-5 1.6506389623423227E-4 1.500580874856657E-5 9.487782720691179 0.0 82-83 6.002323499426628E-5 1.6506389623423227E-4 1.500580874856657E-5 10.186040516283853 0.0 84-85 6.002323499426628E-5 1.7256680060851555E-4 1.500580874856657E-5 10.857858076861554 0.0 86-87 6.002323499426628E-5 1.8006970498279885E-4 1.500580874856657E-5 11.622043893191055 0.0 88 6.002323499426628E-5 1.950755137313654E-4 1.500580874856657E-5 12.240193178779506 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7045 0.0 47.31176 1 GTATCAA 11455 0.0 36.69587 1 TCAACGC 12350 0.0 32.908802 4 ATCAACG 12320 0.0 32.86429 3 CAACGCA 13400 0.0 30.291153 5 TATCAAC 13730 0.0 29.899645 2 AACGCAG 14230 0.0 28.487894 6 GTGGTAT 4195 0.0 24.776114 1 GTACATG 19605 0.0 24.54879 1 ACGCAGA 16815 0.0 23.864676 7 TACATGG 20015 0.0 22.914722 2 CGCAGAG 17355 0.0 22.858116 8 ACATGGG 20530 0.0 21.716393 3 TGGTATC 4660 0.0 20.43098 2 GCAGAGT 19745 0.0 18.945839 9 GTACCGT 450 1.8189894E-12 18.199001 6 CATGGGT 6470 0.0 18.196257 4 CATGGGG 12635 0.0 17.825262 4 GAGTACA 15145 0.0 17.782204 1 TACCTGG 3975 0.0 17.64191 2 >>END_MODULE