FastQCFastQC Report
Wed 25 May 2016
SRR2049503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664086
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269570.404511586435109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255900.3839986458758185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145330.21807941854291796No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72740.10915225283707324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141700.056.5223771
GTATCAA256950.041.743711
GTGGTAT56100.037.6850781
TGGTATC62350.033.058172
ATCAACG324050.032.7475553
TCAACGC327900.032.3774074
TATCAAC330350.032.265252
CAACGCA340050.031.2641355
AACGCAG355000.030.0390686
ACGCAGA406150.026.116547
CGCAGAG407350.025.8896228
GCAGAGT434300.023.882689
TATAACG6250.023.341182
GTACATG210050.023.2456681
TACATGG212950.022.0101432
GAGTACT307200.021.61664212-13
CAGAGTA419850.021.10009810-11
ACATGGG215150.020.9978243
ATAACGC7300.020.6286853
AGAGTAC405750.020.45499810-11
Produced by FastQC (version 0.11.5)