##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049503_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6664086
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76200577243451	31.0	31.0	34.0	30.0	34.0
2	31.97716956233758	33.0	31.0	34.0	30.0	34.0
3	31.985706066818466	33.0	31.0	34.0	30.0	34.0
4	35.62424329457933	37.0	35.0	37.0	33.0	37.0
5	35.74647776154149	37.0	35.0	37.0	35.0	37.0
6	35.80268441913865	37.0	35.0	37.0	35.0	37.0
7	35.79207861363134	37.0	35.0	37.0	35.0	37.0
8	35.78165092707387	37.0	35.0	37.0	33.0	37.0
9	37.52803145097467	39.0	37.0	39.0	35.0	39.0
10-11	37.48209484391408	39.0	37.0	39.0	34.5	39.0
12-13	37.46270823935946	39.0	37.0	39.0	34.5	39.0
14-15	38.720687353074375	40.0	38.0	41.0	34.5	41.0
16-17	38.726043751536224	40.0	38.0	41.0	34.5	41.0
18-19	38.80802033467155	40.0	38.0	41.0	35.0	41.0
20-21	38.83495771213037	40.0	38.0	41.0	35.0	41.0
22-23	38.80116005405692	40.0	38.0	41.0	35.0	41.0
24-25	38.757714486277635	40.0	38.0	41.0	34.0	41.0
26-27	38.65988321579283	40.0	38.0	41.0	34.0	41.0
28-29	38.566648374585796	40.0	38.0	41.0	34.0	41.0
30-31	38.437218172154445	40.0	38.0	41.0	34.0	41.0
32-33	38.27097069275517	40.0	38.0	41.0	34.0	41.0
34-35	38.15481560412036	40.0	38.0	41.0	34.0	41.0
36-37	38.008322971822395	40.0	37.0	41.0	33.0	41.0
38-39	37.909368291465626	40.0	37.0	41.0	33.0	41.0
40-41	37.77824888514344	40.0	37.0	41.0	33.0	41.0
42-43	37.62206355080052	40.0	37.0	41.0	33.0	41.0
44-45	37.41931429156226	40.0	37.0	41.0	32.5	41.0
46-47	37.14380164061508	40.0	37.0	41.0	31.5	41.0
48-49	36.885329886198946	40.0	36.5	41.0	31.0	41.0
50-51	36.66152027449826	40.0	36.0	41.0	31.0	41.0
52-53	36.55320729654449	40.0	36.0	41.0	30.5	41.0
54-55	36.41914345042966	39.0	35.5	41.0	30.0	41.0
56-57	36.240406186234694	39.0	35.0	41.0	30.0	41.0
58-59	36.06477339878267	39.0	35.0	41.0	30.0	41.0
60-61	35.87134049590597	39.0	35.0	41.0	29.5	41.0
62-63	35.66787028258639	38.5	35.0	41.0	29.0	41.0
64-65	35.3850085368046	38.0	35.0	40.0	29.0	41.0
66-67	35.12918080588996	37.0	35.0	40.0	29.0	41.0
68-69	34.81690542408967	37.0	34.0	40.0	28.0	41.0
70-71	34.51940836297731	36.5	34.0	39.0	28.0	41.0
72-73	34.197159670508455	36.0	34.0	39.0	28.0	41.0
74-75	33.86121007441981	35.5	34.0	39.0	28.0	40.5
76-77	33.56492428218964	35.0	34.0	37.5	27.0	40.0
78-79	33.23871585690821	35.0	34.0	37.0	27.0	39.0
80-81	32.93109782796921	35.0	33.5	37.0	26.0	39.0
82-83	32.638652697459186	35.0	33.0	36.0	26.0	38.5
84-85	32.36904978417145	35.0	33.0	36.0	26.0	37.0
86-87	32.09482035495941	35.0	33.0	35.5	26.0	37.0
88-89	31.88684344709837	35.0	33.0	35.0	25.5	36.5
90-91	31.73695537542583	35.0	33.0	35.0	25.0	36.0
92-93	31.57558748491541	35.0	33.0	35.0	25.0	36.0
94-95	31.39341554115598	35.0	33.0	35.0	24.5	36.0
96-97	31.22319174752547	35.0	33.0	35.0	24.0	35.5
98-99	31.03611493008944	35.0	33.0	35.0	23.5	35.0
100	30.919996530657016	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4403107440148837
1101	2	0.4443941089542207
1101	3	0.5099938908159132
1101	4	0.3860799891262232
1101	5	0.30076802376019174
1101	6	0.3049854182088083
1101	7	0.3188605663711357
1101	8	0.3217252481022541
1101	9	0.3487533554508744
1101	10-11	0.35444391948208676
1101	12-13	0.31431010581437846
1101	14-15	0.5121075824833952
1101	16-17	0.539001047338644
1101	18-19	0.5012452000681051
1101	20-21	0.469654950643708
1101	22-23	0.5051383594566516
1101	24-25	0.559944399978825
1101	26-27	0.5532449986922998
1101	28-29	0.5423064737099637
1101	30-31	0.5255219292333067
1101	32-33	0.5668450282662221
1101	34-35	0.5958053110659307
1101	36-37	0.5859182078022798
1101	38-39	0.575824368036784
1101	40-41	0.62865354942317
1101	42-43	0.5342731163090946
1101	44-45	0.5906519152809082
1101	46-47	0.6840055860735816
1101	48-49	0.6559003462173791
1101	50-51	0.6252351257626998
1101	52-53	0.5669437334027521
1101	54-55	0.5653743282410773
1101	56-57	0.5199731533811232
1101	58-59	0.4967091960968304
1101	60-61	0.5657811259390684
1101	62-63	0.5763992623479908
1101	64-65	0.5455293225634179
1101	66-67	0.6126237286427383
1101	68-69	0.5384019548927839
1101	70-71	0.5455659170134837
1101	72-73	0.5426933312424964
1101	74-75	0.5604086032574855
1101	76-77	0.5326913591384468
1101	78-79	0.5342662759148027
1101	80-81	0.5695921514053026
1101	82-83	0.5590047954390229
1101	84-85	0.5287397012634187
1101	86-87	0.6616927035103899
1101	88-89	0.6671359960126289
1101	90-91	0.6444046545105451
1101	92-93	0.6667000897985744
1101	94-95	0.6086994227197557
1101	96-97	0.6839633164111554
1101	98-99	0.7222940038288002
1101	100	0.7498815643641343
1102	1	0.3214960463544436
1102	2	0.3501656442826757
1102	3	0.4257218196501569
1102	4	0.3823903051714055
1102	5	0.3290470536896848
1102	6	0.2744856591199678
1102	7	0.3068967300440164
1102	8	0.3139289741566458
1102	9	0.3878980750118757
1102	10-11	0.3511175010732259
1102	12-13	0.32251992704812693
1102	14-15	0.4159743274521901
1102	16-17	0.45052925407357236
1102	18-19	0.4347977812242618
1102	20-21	0.40302463803357824
1102	22-23	0.3875873058168793
1102	24-25	0.38312490572221947
1102	26-27	0.31045272815174485
1102	28-29	0.3579694991312081
1102	30-31	0.41695726975082437
1102	32-33	0.46940414951293974
1102	34-35	0.48307518403071015
1102	36-37	0.43905348361767693
1102	38-39	0.4645024691683517
1102	40-41	0.47856559762726647
1102	42-43	0.47125102924194806
1102	44-45	0.43794038353754416
1102	46-47	0.464575167912173
1102	48-49	0.5031381055740027
1102	50-51	0.5662689139348487
1102	52-53	0.5199978129524823
1102	54-55	0.5481028218950001
1102	56-57	0.5601730405388992
1102	58-59	0.5323898061428025
1102	60-61	0.5477572600814185
1102	62-63	0.5108881207904759
1102	64-65	0.5242505901581254
1102	66-67	0.5626682823740552
1102	68-69	0.5479200376596012
1102	70-71	0.5173542317332078
1102	72-73	0.5750359896183639
1102	74-75	0.5760155106733436
1102	76-77	0.5201476004765837
1102	78-79	0.4884869468870505
1102	80-81	0.44795111426657286
1102	82-83	0.4619550469653504
1102	84-85	0.48999192249873147
1102	86-87	0.5213628737723397
1102	88-89	0.49475897823171167
1102	90-91	0.5506000181722968
1102	92-93	0.602431731746087
1102	94-95	0.5521420182261885
1102	96-97	0.5501261978216938
1102	98-99	0.6269548995581573
1102	100	0.6891065907662899
1103	1	0.2973873256791997
1103	2	0.350211001241604
1103	3	0.4299981430025568
1103	4	0.363726106489338
1103	5	0.25516562835417744
1103	6	0.25967844418180874
1103	7	0.2738111745529821
1103	8	0.28051920578257494
1103	9	0.354643603949647
1103	10-11	0.3361932126741962
1103	12-13	0.3106986243949308
1103	14-15	0.46239595720726356
1103	16-17	0.4446254425987206
1103	18-19	0.4049851492575769
1103	20-21	0.40042470676613107
1103	22-23	0.3860382260235582
1103	24-25	0.4139880540130818
1103	26-27	0.39952593622732735
1103	28-29	0.3643808337503387
1103	30-31	0.4482598834042477
1103	32-33	0.4117084722746682
1103	34-35	0.4830068804723595
1103	36-37	0.509205756983313
1103	38-39	0.5270802644523798
1103	40-41	0.5495582427170831
1103	42-43	0.5485951008986163
1103	44-45	0.5507418221440972
1103	46-47	0.5706773166545318
1103	48-49	0.5478112078047772
1103	50-51	0.5417738435771611
1103	52-53	0.4934681087359394
1103	54-55	0.5064788356425751
1103	56-57	0.5355675606183681
1103	58-59	0.486933564626419
1103	60-61	0.4990816408298926
1103	62-63	0.48430804912335645
1103	64-65	0.5112834377794968
1103	66-67	0.46527507192649864
1103	68-69	0.46512529959414195
1103	70-71	0.48898782204238245
1103	72-73	0.5029842707091561
1103	74-75	0.4543230132930418
1103	76-77	0.44015588895809543
1103	78-79	0.42856744364719646
1103	80-81	0.3982836857891954
1103	82-83	0.4041420449037503
1103	84-85	0.4110868341249443
1103	86-87	0.43113046024610924
1103	88-89	0.410795583487225
1103	90-91	0.4241196971826682
1103	92-93	0.4353206357738273
1103	94-95	0.47004820157952665
1103	96-97	0.4724609017023056
1103	98-99	0.5045622069172637
1103	100	0.46570234186490467
1104	1	0.2668013814913941
1104	2	0.2445272652001691
1104	3	0.3528163102071815
1104	4	0.26409441786007903
1104	5	0.23712098160653738
1104	6	0.26466406753470295
1104	7	0.18191816566663732
1104	8	0.23880482138498849
1104	9	0.2779383426252622
1104	10-11	0.2775674816241107
1104	12-13	0.27196746176503694
1104	14-15	0.3659598844690919
1104	16-17	0.3493382729468806
1104	18-19	0.33545906014222027
1104	20-21	0.3421941204052956
1104	22-23	0.3358764587173795
1104	24-25	0.3254535252100297
1104	26-27	0.3497140032892361
1104	28-29	0.34384629822991286
1104	30-31	0.3907673674193717
1104	32-33	0.3351634817297793
1104	34-35	0.3758992439390738
1104	36-37	0.3783251352138919
1104	38-39	0.4499671145787545
1104	40-41	0.4450023967418204
1104	42-43	0.41610962432863374
1104	44-45	0.46175916437989173
1104	46-47	0.4688426089612676
1104	48-49	0.5141201089371137
1104	50-51	0.5774821073507042
1104	52-53	0.5549017511062857
1104	54-55	0.5162569793891549
1104	56-57	0.4802587619045724
1104	58-59	0.5320948070853859
1104	60-61	0.5437367426615225
1104	62-63	0.514032927148989
1104	64-65	0.5080416506392424
1104	66-67	0.5526074689103027
1104	68-69	0.5400552848911957
1104	70-71	0.5485825753729117
1104	72-73	0.5552882606831062
1104	74-75	0.5720631317422757
1104	76-77	0.4776897027020084
1104	78-79	0.4674115791609168
1104	80-81	0.5092052226829793
1104	82-83	0.5280089414403193
1104	84-85	0.536260002881157
1104	86-87	0.5174674437865718
1104	88-89	0.45503912985409656
1104	90-91	0.468286429641239
1104	92-93	0.48247579808298013
1104	94-95	0.47955580136288845
1104	96-97	0.4654010371065098
1104	98-99	0.5599066650557774
1104	100	0.6272131765267268
1105	1	0.29121079599196165
1105	2	0.29313925353731207
1105	3	0.32796255763737747
1105	4	0.2815427206216228
1105	5	0.18685445263665912
1105	6	0.23601109519742636
1105	7	0.18314775954237916
1105	8	0.21111956786850783
1105	9	0.2356833804808005
1105	10-11	0.21870700008426525
1105	12-13	0.23655846390067126
1105	14-15	0.293811829399246
1105	16-17	0.3182807778907488
1105	18-19	0.2893515059518137
1105	20-21	0.28485013182289975
1105	22-23	0.23824517214629282
1105	24-25	0.2914873333165602
1105	26-27	0.30940048085921035
1105	28-29	0.33049150148411144
1105	30-31	0.2848913565779654
1105	32-33	0.3320535769429682
1105	34-35	0.32389783889998824
1105	36-37	0.32770863922770843
1105	38-39	0.3973389084561276
1105	40-41	0.34322349315985434
1105	42-43	0.28361063452366153
1105	44-45	0.3749666056205214
1105	46-47	0.39400627163664836
1105	48-49	0.3234168950025591
1105	50-51	0.46611697004912855
1105	52-53	0.45405880152886624
1105	54-55	0.4304197659465743
1105	56-57	0.45720793967598894
1105	58-59	0.4483826967861191
1105	60-61	0.45119539853701696
1105	62-63	0.38444106440844905
1105	64-65	0.4068036723294455
1105	66-67	0.4332652074474481
1105	68-69	0.4197973841318685
1105	70-71	0.4088904244590097
1105	72-73	0.37362601523563654
1105	74-75	0.3681946942240373
1105	76-77	0.36873767570043725
1105	78-79	0.3229143534113206
1105	80-81	0.33842750547996303
1105	82-83	0.33617170509752725
1105	84-85	0.34802724212710245
1105	86-87	0.4301918566730336
1105	88-89	0.4298801026257415
1105	90-91	0.3918274088397098
1105	92-93	0.37881953845658245
1105	94-95	0.39202083236313356
1105	96-97	0.4128622156993913
1105	98-99	0.507133670468356
1105	100	0.5504206251545227
1106	1	0.18290298196906818
1106	2	0.18782461388839522
1106	3	0.2881727000437664
1106	4	0.2223315944344506
1106	5	0.20451887426513338
1106	6	0.20097666995360441
1106	7	0.162875870223246
1106	8	0.19584782631915942
1106	9	0.19193876271479127
1106	10-11	0.22986992998563238
1106	12-13	0.19541077219259506
1106	14-15	0.2541873421318712
1106	16-17	0.273799436256013
1106	18-19	0.26008760883326687
1106	20-21	0.20580840883744855
1106	22-23	0.21896185973093907
1106	24-25	0.2504303350674846
1106	26-27	0.310794096511934
1106	28-29	0.29202229944212377
1106	30-31	0.29078869911843697
1106	32-33	0.2644412908948226
1106	34-35	0.26574674939199383
1106	36-37	0.29519208517499607
1106	38-39	0.27682231945812674
1106	40-41	0.27251918340983394
1106	42-43	0.21234937435789902
1106	44-45	0.2542474382188473
1106	46-47	0.2114081775874581
1106	48-49	0.23858880438768892
1106	50-51	0.2871420395963611
1106	52-53	0.2555604180043787
1106	54-55	0.1396426153344521
1106	56-57	0.20318357914825214
1106	58-59	0.22069639657199502
1106	60-61	0.19848864863759275
1106	62-63	0.1859505071731249
1106	64-65	0.17953713865164644
1106	66-67	0.22349497037551913
1106	68-69	0.19904400536886868
1106	70-71	0.1622450789455101
1106	72-73	0.1877263733107455
1106	74-75	0.18245951751085698
1106	76-77	0.16201095507871344
1106	78-79	0.15755784985455534
1106	80-81	0.12988168667421007
1106	82-83	0.19181744449325322
1106	84-85	0.1585731367657104
1106	86-87	0.20940655786580464
1106	88-89	0.17995350544590494
1106	90-91	0.20266257611216076
1106	92-93	0.2168557337108794
1106	94-95	0.21008360473127752
1106	96-97	0.25102398083641475
1106	98-99	0.3078941358845384
1106	100	0.2885836456460389
1107	1	0.14289190457430223
1107	2	0.1765244613547594
1107	3	0.22543299284857454
1107	4	0.22356718156509459
1107	5	0.16091691798897045
1107	6	0.16237019926886376
1107	7	0.146746789949475
1107	8	0.16765208962384293
1107	9	0.19822849708154422
1107	10-11	0.16349099671423772
1107	12-13	0.13131514420832957
1107	14-15	0.18335977040317175
1107	16-17	0.18468880556567058
1107	18-19	0.19720306042688662
1107	20-21	0.23592730858697308
1107	22-23	0.23448998733120163
1107	24-25	0.24107584463785514
1107	26-27	0.23058246499171275
1107	28-29	0.2662809104286481
1107	30-31	0.2557736082481341
1107	32-33	0.3157714083843288
1107	34-35	0.218118131112071
1107	36-37	0.3220451447967321
1107	38-39	0.2735213186015386
1107	40-41	0.2842511736217759
1107	42-43	0.2328064493291393
1107	44-45	0.25604285857637166
1107	46-47	0.2878358276945221
1107	48-49	0.2760671595479991
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2216	92-93	-1.2526970408356775
2216	94-95	-1.3879360951026811
2216	96-97	-1.4466568020961468
2216	98-99	-1.5168480100514898
2216	100	-1.5094611310091928
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	415.0
3	56.0
4	60.0
5	152.0
6	468.0
7	1171.0
8	2772.0
9	4453.0
10	6339.0
11	8933.0
12	13050.0
13	17783.0
14	20848.0
15	26462.0
16	34773.0
17	48266.0
18	50290.0
19	28313.0
20	20227.0
21	19372.0
22	20251.0
23	21505.0
24	25054.0
25	30276.0
26	38136.0
27	48963.0
28	63473.0
29	82733.0
30	107180.0
31	137978.0
32	178062.0
33	230756.0
34	313951.0
35	455863.0
36	736881.0
37	1338754.0
38	1823000.0
39	704923.0
40	2144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.928387097839774	20.432038974168325	14.917460222603571	22.722113705388335
2	15.892039484602943	20.99102860025696	39.214497575504204	23.902434339635892
3	17.949813372756594	25.66733082376188	31.143565674272512	25.239290129209017
4	12.78099416663111	17.83475767210902	37.744712138539356	31.63953602272051
5	12.628647385215261	37.37777523007368	35.18034043462498	14.813236950086075
6	29.836752707370223	38.09608701400178	17.571124225476854	14.496036053151146
7	26.009478269037945	33.31029941690428	22.470118182748543	18.21010413130923
8	25.036591664633377	35.398672826251044	20.53480402263716	19.029931486478414
9	24.813095036945825	16.668357322133794	21.91857783262571	36.599969808294674
10-11	23.646768664149892	27.05693624001851	28.48524913994207	20.811045955889526
12-13	24.7141036830935	24.67949278261811	28.913186963162463	21.693216571125927
14-15	21.96868902247563	26.521835069768397	27.086128634773466	24.423347272982507
16-17	20.555121887682724	29.69001300403386	27.81866110371325	21.936204004570172
18-19	20.41713597333528	27.715376122096863	31.119436333804817	20.748051570763042
20-21	21.528211070505392	26.959451003483448	31.01562614888223	20.496711777128926
22-23	21.194766994303496	26.883859241912546	31.116750294038827	20.804623469745138
24-25	21.045136572367163	26.91035950016251	31.14962802100693	20.89487590646339
26-27	20.811623679526345	26.887123005315356	31.162015316128873	21.139237999029426
28-29	20.85681684459339	26.939485806953194	31.264843587397678	20.93885376105574
30-31	21.198854212512696	27.215033831462875	30.861420427748058	20.724691528276367
32-33	20.79121733957345	27.059721847806422	31.194827857475715	20.95423295514441
34-35	20.957892800302997	26.7456407375295	31.124133151943116	21.172333310224385
36-37	21.304054299419306	26.99022791722676	30.66402504409457	21.041692739259368
38-39	21.138630263775106	27.006119068691493	30.81172721960671	21.04352344792669
40-41	21.00801220152321	26.88728806921159	30.85817019768352	21.246529531581675
42-43	21.04590186861334	26.899945468891005	30.66810662407418	21.386046038421473
44-45	21.204333197380706	27.044706505888428	30.43012200022629	21.320838296504576
46-47	21.255675572013928	27.279502395377254	29.972940025083712	21.491882007525113
48-49	21.263868743590645	27.200346754228562	29.76387909759868	21.771905404582114
50-51	21.26810038165774	27.254675284802747	29.53614344112606	21.941080892413453
52-53	21.210388041210752	27.373926446927605	29.49499751353749	21.92068799832415
54-55	21.253199613570413	27.626721804010334	29.3022629059709	21.817815676448355
56-57	21.069423473826717	27.431976418071436	29.2826878284584	22.215912279643447
58-59	20.95276538754441	27.043678229911876	29.59379208667369	22.40976429587002
60-61	21.15487405174543	27.26137537840898	29.32172543991779	22.262025129927796
62-63	20.882323547445218	27.239369359879213	29.43200312841101	22.446303964264565
64-65	20.87422041072099	27.028987921224306	29.567828206298657	22.528963461756046
66-67	20.83897176597061	27.078844720791416	29.331239122664382	22.75094439057359
68-69	21.17318864132306	27.267317678673415	28.992407961121753	22.56708571888178
70-71	21.114613466873028	27.092830134545082	28.97743216399068	22.81512423459121
72-73	20.962762185241907	27.198388496186876	29.030980392509942	22.80786892606128
74-75	20.99396676453455	27.22870773276335	28.813598744073833	22.963726758628265
76-77	21.042443029696795	27.33754486361671	28.55914524512439	23.06086686156211
78-79	21.062099470826745	27.44419348853318	28.552066757356933	22.941640283283146
80-81	20.922455865743895	27.335624275827254	28.531477650405655	23.210442208023192
82-83	20.843922771213595	27.28335141162618	28.597135729151685	23.275590088008535
84-85	20.87536085218588	27.192086056512476	28.381228873697005	23.551324217604634
86-87	20.967428991762713	27.3763498850405	28.272459268983024	23.383761854213766
88-89	20.83862694697628	27.267132832294195	28.26406661921328	23.630173601516248
90-91	20.74411438152856	27.16673846949385	28.197337878294732	23.891809270682852
92-93	20.809203190198375	27.23551805009486	28.036421466447443	23.918857293259325
94-95	20.70782812750177	27.053782596814873	28.17762156940099	24.060767706282366
96-97	20.71996225138468	27.097543991594947	28.099354367527248	24.083139389493127
98-99	20.675213628400666	27.143589962520725	27.999011416948782	24.182184992129827
100	20.786400262544586	27.11832258380126	27.85450084040978	24.240776313244382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1182.0
1	1036.0
2	2512.0
3	3936.0
4	2403.5
5	1233.5
6	2561.0
7	5938.0
8	9821.5
9	11480.0
10	8994.5
11	5819.5
12	5183.5
13	5523.0
14	6167.0
15	6937.0
16	7690.0
17	8491.5
18	9432.0
19	10591.0
20	12086.0
21	14163.0
22	17410.5
23	22386.5
24	28782.5
25	36208.5
26	46570.5
27	59807.0
28	76830.5
29	95356.5
30	111375.0
31	126541.5
32	141172.5
33	155804.0
34	170786.0
35	183996.5
36	195401.5
37	205838.5
38	215275.5
39	222483.0
40	228318.5
41	231836.5
42	231704.5
43	232004.5
44	230939.0
45	227624.0
46	224341.5
47	219460.5
48	213915.5
49	211533.5
50	208205.0
51	200101.0
52	189640.5
53	182234.5
54	177130.0
55	168254.5
56	155037.0
57	138979.5
58	124172.5
59	111838.5
60	97494.5
61	81704.5
62	68628.0
63	56635.5
64	45277.5
65	35938.5
66	28338.5
67	22235.0
68	17110.0
69	13015.5
70	9751.5
71	7269.5
72	5527.0
73	4033.0
74	3020.0
75	2157.0
76	1373.5
77	929.5
78	660.5
79	492.5
80	299.0
81	128.0
82	74.5
83	41.5
84	20.5
85	13.5
86	8.5
87	6.5
88	4.5
89	1.5
90	0.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	0.5
97	0.5
98	1.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06129872873789444
2	3.0011617497133143E-4
3	0.0
4	0.11851587749617876
5	0.0020107783723079206
6	0.002205853886039286
7	0.0
8	0.0
9	6.002323499426628E-5
10-11	0.0
12-13	7.502904374283285E-6
14-15	2.2508713122849856E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.950755137313654E-4
30-31	3.4513360121703115E-4
32-33	7.502904374283285E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.6506389623423227E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.7256680060851557E-4
80-81	2.4759584435134836E-4
82-83	2.4759584435134836E-4
84-85	0.0
86-87	0.0
88-89	3.751452187141643E-5
90-91	4.501742624569971E-5
92-93	7.352846286797619E-4
94-95	7.953078636740282E-4
96-97	7.502904374283285E-6
98-99	3.751452187141643E-5
100	0.001125435656142493
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6664086.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.70110617200108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3505807697165	44.23162441885072
2	14.62302338502289	17.167752965597664
3	4.479888019016817	7.889231467289454
4	1.9492037439634415	4.5768166370104
5	1.0166221264988882	2.9838421692208374
6	0.5886228144675001	2.0731686196391297
7	0.38702385583547366	1.5903109916746767
8	0.2757655002106001	1.2950191925149939
9	0.20507763504867202	1.083445562564549
>10	1.0482558811041705	10.71902474169281
>50	0.0517016951516359	2.0371774427418385
>100	0.022778300738908023	2.3070499970303
>500	8.686808875845843E-4	0.3325422075703015
>1k	4.5985487317255666E-4	0.5173098179806738
>5k	5.1094985481723206E-5	0.1897077596019326
>10k+	7.664247822258481E-5	1.0059760090195762
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26957	0.404511586435109	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25590	0.3839986458758185	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14533	0.21807941854291796	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7274	0.10915225283707324	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.500580874856657E-5	0.0	1.500580874856657E-5	0.0
6	0.0	1.500580874856657E-5	0.0	1.500580874856657E-5	0.0
7	0.0	1.500580874856657E-5	0.0	1.500580874856657E-5	0.0
8	0.0	1.500580874856657E-5	0.0	1.500580874856657E-5	0.0
9	1.500580874856657E-5	1.500580874856657E-5	0.0	4.501742624569971E-5	0.0
10-11	2.2508713122849856E-5	1.500580874856657E-5	0.0	5.2520330619982996E-5	0.0
12-13	3.001161749713314E-5	1.500580874856657E-5	0.0	8.253194811711613E-5	0.0
14-15	3.001161749713314E-5	2.2508713122849856E-5	0.0	9.75377568656827E-5	0.0
16-17	3.001161749713314E-5	3.001161749713314E-5	0.0	1.4255518311138241E-4	0.0
18-19	7.502904374283285E-5	3.001161749713314E-5	0.0	1.8757260935708213E-4	0.0
20-21	9.003485249139942E-5	3.001161749713314E-5	0.0	3.226248880941813E-4	0.0
22-23	9.003485249139942E-5	3.001161749713314E-5	0.0	6.977701068083455E-4	0.0
24-25	9.003485249139942E-5	3.001161749713314E-5	0.0	0.0018081999542022718	0.0
26-27	9.003485249139942E-5	3.001161749713314E-5	0.0	0.004216632258347206	0.0
28-29	9.003485249139942E-5	3.001161749713314E-5	0.0	0.012094681851344656	0.0
30-31	9.003485249139942E-5	3.001161749713314E-5	0.0	0.0334854622224263	0.0
32-33	9.003485249139942E-5	3.001161749713314E-5	0.0	0.08420509579258131	0.0
34-35	9.003485249139942E-5	3.001161749713314E-5	0.0	0.16965567371129364	0.0
36-37	9.003485249139942E-5	3.001161749713314E-5	0.0	0.3055407748339382	0.0
38-39	9.003485249139942E-5	3.001161749713314E-5	0.0	0.49148525394180087	0.0
40-41	9.003485249139942E-5	3.001161749713314E-5	0.0	0.6926156115032129	0.0
42-43	9.003485249139942E-5	4.501742624569971E-5	0.0	0.904182509049253	0.0
44-45	9.003485249139942E-5	4.501742624569971E-5	0.0	1.1683297604502703	0.0
46-47	9.003485249139942E-5	5.2520330619982996E-5	0.0	1.4913222908587915	0.0
48-49	9.003485249139942E-5	6.002323499426628E-5	0.0	1.8284352872997136	0.0
50-51	9.003485249139942E-5	8.253194811711613E-5	0.0	2.1736439175604874	0.0
52-53	9.003485249139942E-5	9.003485249139942E-5	0.0	2.513960654169229	0.0
54-55	9.003485249139942E-5	9.003485249139942E-5	0.0	2.899744991286127	0.0
56-57	9.003485249139942E-5	9.003485249139942E-5	0.0	3.3498817392212525	0.0
58-59	9.003485249139942E-5	9.003485249139942E-5	0.0	3.841892196469253	0.0
60-61	9.003485249139942E-5	9.003485249139942E-5	0.0	4.337924210461869	0.0
62-63	9.003485249139942E-5	9.003485249139942E-5	0.0	4.82690349434266	0.0
64-65	9.003485249139942E-5	9.003485249139942E-5	0.0	5.34760505791792	0.0
66-67	9.003485249139942E-5	9.003485249139942E-5	0.0	5.936605560012281	0.0
68-69	9.003485249139942E-5	9.003485249139942E-5	0.0	6.574585021861963	0.0
70-71	9.003485249139942E-5	9.003485249139942E-5	0.0	7.242148435659443	0.0
72-73	9.003485249139942E-5	9.003485249139942E-5	0.0	7.916809596994996	0.0
74-75	1.2004646998853256E-4	9.003485249139942E-5	0.0	8.587996913605256	0.0
76-77	1.2004646998853256E-4	9.003485249139942E-5	0.0	9.291379192885566	0.0
78-79	1.2004646998853256E-4	9.003485249139942E-5	0.0	10.057260665603655	0.0
80-81	1.2004646998853256E-4	9.003485249139942E-5	0.0	10.854684348311231	0.0
82-83	1.2754937436281583E-4	9.003485249139942E-5	0.0	11.657397578602676	0.0
84-85	1.3505227873709914E-4	1.0504066123996599E-4	0.0	12.429956636213877	0.0
86-87	1.3505227873709914E-4	1.0504066123996599E-4	0.0	13.224559226876725	0.0
88	1.3505227873709914E-4	1.0504066123996599E-4	0.0	13.848560777877116	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14170	0.0	56.522377	1
GTATCAA	25695	0.0	41.74371	1
GTGGTAT	5610	0.0	37.685078	1
TGGTATC	6235	0.0	33.05817	2
ATCAACG	32405	0.0	32.747555	3
TCAACGC	32790	0.0	32.377407	4
TATCAAC	33035	0.0	32.26525	2
CAACGCA	34005	0.0	31.264135	5
AACGCAG	35500	0.0	30.039068	6
ACGCAGA	40615	0.0	26.11654	7
CGCAGAG	40735	0.0	25.889622	8
GCAGAGT	43430	0.0	23.88268	9
TATAACG	625	0.0	23.34118	2
GTACATG	21005	0.0	23.245668	1
TACATGG	21295	0.0	22.010143	2
GAGTACT	30720	0.0	21.616642	12-13
CAGAGTA	41985	0.0	21.100098	10-11
ACATGGG	21515	0.0	20.997824	3
ATAACGC	730	0.0	20.628685	3
AGAGTAC	40575	0.0	20.454998	10-11
>>END_MODULE