FastQCFastQC Report
Wed 25 May 2016
SRR2049502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5582339
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.043.7957231
GTATCAA92650.036.3638951
TCAACGC97550.033.006124
ATCAACG97650.032.9723173
CAACGCA106050.030.4082985
TATCAAC110100.029.615782
AACGCAG113400.028.3916766
ACGCAGA133800.023.5655777
GTACATG162350.023.0229241
CGCAGAG138350.022.8213278
GTGGTAT35500.022.2117251
TACATGG162850.022.1287022
ACATGGG168150.020.731053
TGGTATC35200.020.6498412
TACCTGG37250.019.9256632
GCAGAGT158650.019.0743899
TCTGTCG15700.018.1512058
GTATAGG16750.016.8127121
CATGGGG105350.016.714564
GTCTCGC15700.016.6326121
Produced by FastQC (version 0.11.5)