##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049502_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5582339 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.733505614761125 31.0 31.0 34.0 27.0 34.0 2 30.93101153477064 31.0 31.0 34.0 27.0 34.0 3 31.049420502767745 31.0 31.0 34.0 27.0 34.0 4 34.63319515350107 37.0 35.0 37.0 32.0 37.0 5 34.664517686940904 37.0 35.0 37.0 32.0 37.0 6 34.65404035835158 37.0 35.0 37.0 32.0 37.0 7 30.685495273576183 37.0 33.0 37.0 2.0 37.0 8 32.448307958366556 37.0 33.0 37.0 17.0 37.0 9 35.236805217311236 39.0 34.0 39.0 28.0 39.0 10-11 35.76871693030466 39.0 36.0 39.0 30.0 39.0 12-13 35.923274903226044 39.0 37.0 39.0 30.0 39.0 14-15 37.10502344984781 40.0 37.0 41.0 31.0 41.0 16-17 37.12643803968193 40.0 37.0 41.0 31.0 41.0 18-19 37.1364579435251 40.0 37.0 41.0 31.0 41.0 20-21 37.208597417677424 40.0 37.0 41.0 31.0 41.0 22-23 37.167317946831965 40.0 37.0 41.0 31.0 41.0 24-25 37.14736546813083 40.0 37.0 41.0 31.0 41.0 26-27 37.171471492505205 40.0 37.0 41.0 31.0 41.0 28-29 37.14581146003495 40.0 37.0 41.0 31.0 41.0 30-31 37.058781722141916 40.0 37.0 41.0 30.0 41.0 32-33 36.98950887790942 40.0 37.0 41.0 30.0 41.0 34-35 36.91932422950308 40.0 37.0 41.0 30.0 41.0 36-37 36.74104609196969 40.0 37.0 41.0 30.0 41.0 38-39 36.61988415608583 40.0 36.5 41.0 30.0 41.0 40-41 36.52996503795273 40.0 36.0 41.0 30.0 41.0 42-43 36.40675485670074 40.0 36.0 41.0 30.0 41.0 44-45 36.291476744783864 40.0 36.0 41.0 29.5 41.0 46-47 36.12785026133311 40.0 35.5 41.0 29.0 41.0 48-49 36.039207669043385 40.0 35.0 41.0 29.0 41.0 50-51 35.90519108567215 39.0 35.0 41.0 28.0 41.0 52-53 35.77853709708421 39.0 35.0 41.0 28.0 41.0 54-55 35.64987830370029 39.0 35.0 41.0 28.0 41.0 56-57 35.450016829862896 39.0 35.0 41.0 28.0 41.0 58-59 35.26403466360606 39.0 35.0 41.0 27.5 41.0 60-61 35.033435626177486 38.5 34.0 40.0 27.0 41.0 62-63 34.78095525907689 38.0 34.0 40.0 26.5 41.0 64-65 34.41247432303914 37.5 34.0 40.0 26.0 41.0 66-67 30.930688014468487 36.0 30.0 40.0 6.5 41.0 68-69 31.269781985651534 36.0 30.0 39.0 11.0 41.0 70-71 31.408490777790455 35.0 29.0 39.0 15.0 41.0 72-73 32.612143852245445 35.0 31.5 39.0 22.5 40.5 74-75 32.67123171846067 35.0 33.0 38.5 23.0 40.0 76-77 32.34293492387331 35.0 32.5 37.0 22.0 39.5 78-79 28.987157354650087 35.0 28.5 36.5 6.0 39.0 80-81 30.696158366591497 35.0 29.5 36.0 15.0 39.0 82-83 31.04401792868545 35.0 31.5 36.0 16.5 37.5 84-85 29.188937199980153 34.5 29.0 35.5 7.0 37.0 86-87 28.766070190291202 34.0 26.5 35.0 4.5 37.0 88-89 29.90835929168759 34.0 30.5 35.0 2.0 36.0 90-91 29.977181339219996 35.0 31.0 35.0 2.0 36.0 92-93 29.7530417805153 35.0 31.0 35.0 2.0 36.0 94-95 29.475367404236827 35.0 31.0 35.0 2.0 36.0 96-97 29.20355669908259 34.0 31.0 35.0 2.0 35.0 98-99 28.934131642668063 34.0 30.5 35.0 2.0 35.0 100 28.686428036706477 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3546560931869891 1101 2 0.2985078322833665 1101 3 0.33233975144871053 1101 4 0.3505996404535381 1101 5 0.368958012266269 1101 6 0.3477486606414857 1101 7 -12.018375953515669 1101 8 -6.484049728864246 1101 9 -2.1809923929155275 1101 10-11 -0.1491091606482584 1101 12-13 0.3786720246321096 1101 14-15 0.5557987973111693 1101 16-17 0.651113455792661 1101 18-19 0.6022075819740138 1101 20-21 0.6174446751841245 1101 22-23 0.5820975986930819 1101 24-25 0.6821604621827717 1101 26-27 0.6064935791258748 1101 28-29 0.5608644532072802 1101 30-31 0.640598410851382 1101 32-33 0.7159974841008179 1101 34-35 0.7233256072852612 1101 36-37 0.7840535210316872 1101 38-39 0.8248485115704511 1101 40-41 0.7851447806927823 1101 42-43 0.7888307626980051 1101 44-45 0.6677189359115445 1101 46-47 0.7199036226648303 1101 48-49 0.6481133323436765 1101 50-51 0.7432989069780405 1101 52-53 0.7865068261669705 1101 54-55 0.7126293197784292 1101 56-57 0.6113260900585118 1101 58-59 0.5633710816708586 1101 60-61 0.5539528279848014 1101 62-63 0.5806331234097257 1101 64-65 0.457934136354325 1101 66-67 -10.093592387280559 1101 68-69 -8.010123652431261 1101 70-71 -6.477176157133535 1101 72-73 -1.396051635151501 1101 74-75 -0.13040671821811145 1101 76-77 0.1325953654870986 1101 78-79 3.2349250710571305 1101 80-81 1.1744840812209532 1101 82-83 0.5022365879456316 1101 84-85 -5.195499818870637 1101 86-87 -5.657020495228672 1101 88-89 -1.160606347862167 1101 90-91 0.0854418167790385 1101 92-93 0.4925203392543622 1101 94-95 0.5939449070498775 1101 96-97 0.6555278060763534 1101 98-99 0.6969744411027818 1101 100 0.6781898773188679 1102 1 0.15442402158767976 1102 2 0.18012299098158024 1102 3 0.25079714618411586 1102 4 0.2214500561449526 1102 5 0.20823824244949662 1102 6 0.23196630106468064 1102 7 -12.154092120507833 1102 8 -6.6026571998801415 1102 9 -2.301642789723182 1102 10-11 -0.2496595231669545 1102 12-13 0.2514793952658465 1102 14-15 0.40705034404104623 1102 16-17 0.4458712217432321 1102 18-19 0.41158188315026933 1102 20-21 0.35842369929075346 1102 22-23 0.3515068845387077 1102 24-25 0.40036541072449694 1102 26-27 0.41977259677226897 1102 28-29 0.40666057805882616 1102 30-31 0.4185660202157422 1102 32-33 0.4707124634032098 1102 34-35 0.45496362528447065 1102 36-37 0.585856921104245 1102 38-39 0.5877423996890769 1102 40-41 0.5237817203293176 1102 42-43 0.4560246841117035 1102 44-45 0.48776828218522894 1102 46-47 0.49419684786374063 1102 48-49 0.43292034517966727 1102 50-51 0.46964524526588036 1102 52-53 0.49508726357471744 1102 54-55 0.4974231636692821 1102 56-57 0.49921910852981455 1102 58-59 0.5052289465127586 1102 60-61 0.37338497313142227 1102 62-63 0.3493126509179234 1102 64-65 0.20221235392517656 1102 66-67 -10.141595677032136 1102 68-69 -6.5983068846370365 1102 70-71 -6.168358678713211 1102 72-73 -1.1184152371810043 1102 74-75 -0.17355787286128077 1102 76-77 -8.962436586088529E-4 1102 78-79 3.097698450904172 1102 80-81 1.034520308243632 1102 82-83 0.3933219574960489 1102 84-85 -5.364419120568684 1102 86-87 -5.802293092393125 1102 88-89 -1.3668064363177272 1102 90-91 -0.05071290093344061 1102 92-93 0.31685878611394 1102 94-95 0.28851656847381335 1102 96-97 0.3871616000742968 1102 98-99 0.42447368326725865 1102 100 0.5225692224495866 1103 1 0.3216313904711292 1103 2 0.27375175731850376 1103 3 0.27490443604894566 1103 4 0.31423552282204525 1103 5 0.32913091126358296 1103 6 0.3556425802858385 1103 7 -12.067098756673186 1103 8 -6.533934682371129 1103 9 -2.178650628649798 1103 10-11 -0.19275426223070014 1103 12-13 0.3710445131548923 1103 14-15 0.5292636981239198 1103 16-17 0.500604058715048 1103 18-19 0.548860823321796 1103 20-21 0.5762334977265269 1103 22-23 0.6362028281436949 1103 24-25 0.6856215410244815 1103 26-27 0.5871957888320125 1103 28-29 0.6014881801584409 1103 30-31 0.5914327400276775 1103 32-33 0.6551290606186768 1103 34-35 0.6080584469903698 1103 36-37 0.7300686643007239 1103 38-39 0.7780649552134875 1103 40-41 0.7528058054329492 1103 42-43 0.6877770252687085 1103 44-45 0.6598756476994154 1103 46-47 0.6502062201202534 1103 48-49 0.6270090151304117 1103 50-51 0.6356179879747827 1103 52-53 0.6149198559893136 1103 54-55 0.5701020156044052 1103 56-57 0.5696158290346744 1103 58-59 0.629315528159708 1103 60-61 0.6105327029831074 1103 62-63 0.5112526152037873 1103 64-65 0.39954552324560666 1103 66-67 -10.176381541663009 1103 68-69 -8.08638750142725 1103 70-71 -6.579484188631223 1103 72-73 -1.3775535051412149 1103 74-75 -0.15770655055495553 1103 76-77 0.12859564374208077 1103 78-79 3.2044093438399877 1103 80-81 1.1629176638216876 1103 82-83 0.48462282136089385 1103 84-85 -5.244637067012754 1103 86-87 -5.72771790487489 1103 88-89 -1.1686568101463521 1103 90-91 0.11507014623552436 1103 92-93 0.5256989860571082 1103 94-95 0.6316468436658482 1103 96-97 0.7235070970619901 1103 98-99 0.6883051358557708 1103 100 0.7820017123879062 1104 1 0.22916963363623566 1104 2 0.25663718663807344 1104 3 0.2882127032494388 1104 4 0.24945375969460315 1104 5 0.284827772372914 1104 6 0.3214780456614932 1104 7 -12.104706271949734 1104 8 -6.561194852378112 1104 9 -2.267041560650007 1104 10-11 -0.26986651523451854 1104 12-13 0.31613422104958033 1104 14-15 0.49258385392462145 1104 16-17 0.5331931886916763 1104 18-19 0.5446406854740786 1104 20-21 0.5628529382297884 1104 22-23 0.5629533794925408 1104 24-25 0.657890210179751 1104 26-27 0.6400232642277075 1104 28-29 0.5743330111091538 1104 30-31 0.5245570971876461 1104 32-33 0.5800230239117212 1104 34-35 0.6122388510947019 1104 36-37 0.6509513508361664 1104 38-39 0.6899533602857133 1104 40-41 0.688719165157373 1104 42-43 0.6082260931243937 1104 44-45 0.557749194423117 1104 46-47 0.5856809175163491 1104 48-49 0.5269659339654993 1104 50-51 0.5512093017311486 1104 52-53 0.5560054172843536 1104 54-55 0.5044193907936219 1104 56-57 0.5260198517046746 1104 58-59 0.42122523063848405 1104 60-61 0.4056005400564473 1104 62-63 0.33211201989714567 1104 64-65 0.22391232018651408 1104 66-67 -10.181139773768113 1104 68-69 -8.16719575812014 1104 70-71 -6.63480211561961 1104 72-73 -1.6369362976533175 1104 74-75 -0.3704717098712038 1104 76-77 -0.06502103356228162 1104 78-79 2.9770531643797646 1104 80-81 0.9549610830311472 1104 82-83 0.3118014223608583 1104 84-85 -5.315071709433077 1104 86-87 -5.806810375368041 1104 88-89 -1.3551249261814924 1104 90-91 -0.028714832818323544 1104 92-93 0.2840770567534463 1104 94-95 0.3664597273058696 1104 96-97 0.435364669830129 1104 98-99 0.5084793248640977 1104 100 0.5735300039540228 1105 1 0.132102589887662 1105 2 0.09753306601484013 1105 3 0.12035607687291972 1105 4 0.1863223749791132 1105 5 0.2365618018515505 1105 6 0.2277164881869851 1105 7 -12.350394678335974 1105 8 -6.779009511748914 1105 9 -2.4854817607965813 1105 10-11 -0.3740949302861196 1105 12-13 0.14390522696410812 1105 14-15 0.32090738824151543 1105 16-17 0.3320814623490662 1105 18-19 0.37485532516002706 1105 20-21 0.38676567765144654 1105 22-23 0.3721980784330583 1105 24-25 0.41772312350726537 1105 26-27 0.41604612455434875 1105 28-29 0.36534455336674654 1105 30-31 0.4468753350137291 1105 32-33 0.38101399348697385 1105 34-35 0.4797154073453811 1105 36-37 0.5147408038146253 1105 38-39 0.49205894130031425 1105 40-41 0.5236545929510257 1105 42-43 0.4402099590302768 1105 44-45 0.4172182546024956 1105 46-47 0.4301205662568961 1105 48-49 0.3452525692620725 1105 50-51 0.44848539628002015 1105 52-53 0.38533469920320584 1105 54-55 0.36080746983289913 1105 56-57 0.35035365696596443 1105 58-59 0.3555266206507497 1105 60-61 0.3015809024044529 1105 62-63 0.28623816870165797 1105 64-65 0.0708099490398979 1105 66-67 -10.442924523391362 1105 68-69 -8.381732343233306 1105 70-71 -6.884521383475587 1105 72-73 -1.6786478751796068 1105 74-75 -0.4851160436347328 1105 76-77 -0.23454204418936797 1105 78-79 2.8863271891415216 1105 80-81 0.7974122310046639 1105 82-83 0.12749857900808337 1105 84-85 -5.538711738862503 1105 86-87 -6.011606686818627 1105 88-89 -1.5170772056741235 1105 90-91 -0.18027057626890297 1105 92-93 0.1320717490388148 1105 94-95 0.10864368366421218 1105 96-97 0.21366145404806147 1105 98-99 0.2264117263999168 1105 100 0.3642449424381482 1106 1 0.11475327157210202 1106 2 0.13201967046446583 1106 3 0.11621558746462668 1106 4 0.10095266668204772 1106 5 0.11975481948331179 1106 6 0.1458045011097795 1106 7 -12.30873650963498 1106 8 -6.761343875808198 1106 9 -2.5430704242542603 1106 10-11 -0.42432796680091656 1106 12-13 0.11126766539895527 1106 14-15 0.24593741741087882 1106 16-17 0.3016954517053989 1106 18-19 0.3109966808409297 1106 20-21 0.30763435194968025 1106 22-23 0.2858019498682509 1106 24-25 0.37700071149772896 1106 26-27 0.3138366339397507 1106 28-29 0.3067660574746469 1106 30-31 0.2602915791826774 1106 32-33 0.33364754357967286 1106 34-35 0.3261715326992771 1106 36-37 0.36126381030423715 1106 38-39 0.3480206806570152 1106 40-41 0.3072771055488417 1106 42-43 0.3013521880598944 1106 44-45 0.27024818707892706 1106 46-47 0.3351597095162546 1106 48-49 0.2918329299543245 1106 50-51 0.37603676889878557 1106 52-53 0.32289113103375655 1106 54-55 0.22569117642004954 1106 56-57 0.29040778937367406 1106 58-59 0.22614876915671545 1106 60-61 0.20726504666667012 1106 62-63 0.14626356511021044 1106 64-65 0.013276261540809742 1106 66-67 -10.42542240568645 1106 68-69 -8.396061535839962 1106 70-71 -6.884395360267135 1106 72-73 -1.7854436852439406 1106 74-75 -0.5814428066082797 1106 76-77 -0.29015608119431135 1106 78-79 2.770511658200139 1106 80-81 0.7178526329552639 1106 82-83 0.004343659897063645 1106 84-85 -5.634841222025273 1106 86-87 -6.061857448509205 1106 88-89 -1.6721455640706502 1106 90-91 -0.43953481734156696 1106 92-93 -0.12053821680030552 1106 94-95 -0.03526442254855766 1106 96-97 0.02693074673999618 1106 98-99 0.0857746618096158 1106 100 0.08095922911545728 1107 1 0.025136977914240077 1107 2 0.045817645578839716 1107 3 0.04064934607852422 1107 4 0.05393953375667593 1107 5 0.07494915181390382 1107 6 0.022741846060981175 1107 7 -12.218024440978336 1107 8 -6.710850800026439 1107 9 -2.5285655036292525 1107 10-11 -0.529737405062555 1107 12-13 0.05346766210241327 1107 14-15 0.1764826217436095 1107 16-17 0.22951572871735948 1107 18-19 0.24015094157717698 1107 20-21 0.22064662939613555 1107 22-23 0.30291262252164586 1107 24-25 0.32149601811774886 1107 26-27 0.23225471534859565 1107 28-29 0.2645927775992831 1107 30-31 0.2532351143317584 1107 32-33 0.2362557342363658 1107 34-35 0.19910928404349448 1107 36-37 0.2896199100229211 1107 38-39 0.2633167436436139 1107 40-41 0.22792372169199382 1107 42-43 0.19819564379481136 1107 44-45 0.16888527757514282 1107 46-47 0.16226327974914767 1107 48-49 0.15553920318716052 1107 50-51 0.25154297679529236 1107 52-53 0.24396504754648163 1107 54-55 0.24156889616612887 1107 56-57 0.1967497023553051 1107 58-59 0.2396314046370378 1107 60-61 0.2507397907187823 1107 62-63 0.15214742803794934 1107 64-65 0.06374487673228657 1107 66-67 -10.320658852655 1107 68-69 -8.245796259015794 1107 70-71 -6.738450393825634 1107 72-73 -1.6873686614183363 1107 74-75 -0.4498782961754131 1107 76-77 -0.18087878254056733 1107 78-79 2.9413865664307046 1107 80-81 0.8813879252434447 1107 82-83 0.223251331402885 1107 84-85 -5.406514277762991 1107 86-87 -5.868977013711909 1107 88-89 -1.415691372858948 1107 90-91 -0.17526051934902398 1107 92-93 0.19701084826608195 1107 94-95 0.31190709286776297 1107 96-97 0.33635726782933517 1107 98-99 0.4403569592325596 1107 100 0.5669175127642703 1108 1 0.01700797345979055 1108 2 0.05443847497984322 1108 3 0.04005861579211256 1108 4 -0.015406989074328692 1108 5 -0.019538107170554042 1108 6 0.037524764202906624 1108 7 -12.270257386627815 1108 8 -6.75713128116092 1108 9 -2.6880773419415362 1108 10-11 -0.5981883961952335 1108 12-13 -0.027099425198898075 1108 14-15 0.19021148549501277 1108 16-17 0.14190644348193615 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-0.9097606362777739 2216 5 -0.8147929192393804 2216 6 -0.9891687024518916 2216 7 3.110145696523567 2216 8 1.1886473356144265 2216 9 -0.046351040854958114 2216 10-11 -0.7451291066875498 2216 12-13 -1.192952100053418 2216 14-15 -1.3024234644490136 2216 16-17 -1.5290083640878862 2216 18-19 -1.9359315039600204 2216 20-21 -1.5483277460601315 2216 22-23 -1.378271660030407 2216 24-25 -1.411819969967091 2216 26-27 -1.7915606807020978 2216 28-29 -2.0281197565364195 2216 30-31 -1.9078942475518517 2216 32-33 -2.873677264639767 2216 34-35 -1.8887875331544208 2216 36-37 -1.5607939527078045 2216 38-39 -1.7855068658924012 2216 40-41 -1.8095641812725631 2216 42-43 -1.458304411556945 2216 44-45 -1.607789406167413 2216 46-47 -1.8705294812624018 2216 48-49 -1.9490662791357352 2216 50-51 -2.4315996480815656 2216 52-53 -2.179467324605014 2216 54-55 -1.7859259117377917 2216 56-57 -1.8089117995085502 2216 58-59 -2.154547262135736 2216 60-61 -2.291704185403951 2216 62-63 -2.167608554924925 2216 64-65 -1.8881314072624917 2216 66-67 1.5223457065945851 2216 68-69 0.7134883719206826 2216 70-71 0.3536453126641952 2216 72-73 -1.4161088964260458 2216 74-75 -2.1249929406156767 2216 76-77 -1.8008020451205944 2216 78-79 0.7130865818883834 2216 80-81 -0.6932829869278727 2216 82-83 -1.5260136254304015 2216 84-85 0.20964187742800888 2216 86-87 0.16771711456436478 2216 88-89 -0.9535291396888148 2216 90-91 -1.218768932559776 2216 92-93 -1.3545434517995751 2216 94-95 -1.305872143951504 2216 96-97 -1.2736905285333684 2216 98-99 -1.308517767771086 2216 100 -2.419105124825638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83462.0 3 16038.0 4 9611.0 5 8908.0 6 8446.0 7 9749.0 8 11861.0 9 13157.0 10 14410.0 11 16055.0 12 18158.0 13 20196.0 14 22570.0 15 23758.0 16 24939.0 17 25820.0 18 24120.0 19 22601.0 20 22008.0 21 21697.0 22 23685.0 23 27888.0 24 33045.0 25 40815.0 26 50926.0 27 62255.0 28 77702.0 29 99532.0 30 122344.0 31 148063.0 32 195186.0 33 269489.0 34 398404.0 35 542031.0 36 599055.0 37 994621.0 38 1110627.0 39 368257.0 40 850.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78234330167587 20.477542292860928 14.122871739815187 22.61724266564801 2 16.001197382526097 20.756798065843856 39.57064413590686 23.67136041572319 3 18.32874169532746 25.69242253435846 30.35353671969846 25.62529905061562 4 12.999272508655643 17.7082607439329 38.45722392097592 30.835242826435543 5 12.985206228209812 37.508612174309334 34.90373174514628 14.602449852334576 6 30.052444467534144 37.90510552041841 18.18066514453803 13.861784867509423 7 26.688305212143426 32.71197517540438 22.539027627660253 18.060691984791934 8 24.63484379391642 35.734496143819335 21.177814954561143 18.4528451077031 9 24.469366693781943 17.820039234449933 22.08894873636302 35.621645335405105 10-11 23.420353009733017 27.461168159081705 28.859345518070473 20.25913331311481 12-13 24.117112916288317 25.069124250605345 28.78581003410936 22.02795279899698 14-15 21.751240832919677 27.524224164816935 26.040589795782736 24.683945206480654 16-17 21.00197821083969 30.59994622304681 26.63754136499235 21.76053420112115 18-19 20.878524217178498 29.47741976974168 28.63868532527315 21.00537068780667 20-21 22.293873795005446 28.895793402711067 27.94565395846203 20.864678843821455 22-23 21.91739178840552 28.907007341099078 27.91407221185172 21.261528658643687 24-25 21.571782007506172 28.903054794773304 28.118383709767535 21.40677948795299 26-27 21.387559967178547 28.74620599357848 28.145273532557987 21.720960506684982 28-29 21.418011338974576 28.793754732559236 28.273247110216705 21.514986818249483 30-31 21.84777042811001 29.266274340045843 27.708110140579972 21.17784509126417 32-33 21.280565712839827 29.05819470786207 28.20117020871633 21.46006937058177 34-35 21.35071292318884 28.536283258605028 28.308914184363942 21.804089633842192 36-37 21.91135378528328 29.003151279038804 27.708424599627772 21.377070336050146 38-39 21.73461970042649 29.000538484247457 27.910412415160962 21.354429400165092 40-41 21.434995387685646 28.719657541369088 28.17630102509907 21.669046045846198 42-43 21.28227322755205 28.757459242868933 28.15653697663487 21.803730552944145 44-45 21.60432572333417 28.840905310715883 28.00616373836249 21.54860522758746 46-47 21.379600673023997 29.095354172833886 27.875760163768575 21.649284990373534 48-49 21.21287657701829 28.67155259372899 28.233220016656247 21.882350812596478 50-51 21.26181063167113 28.49541807188653 28.226273291986665 22.01649800445568 52-53 21.19151936525293 28.56252545985377 28.192132730525206 22.053822444368095 54-55 21.320953390433957 28.949651479343046 28.00034000267631 21.729055127546683 56-57 21.065522084324574 28.699043753045373 28.02703753331996 22.208396629310098 58-59 20.901426692408663 28.29461061646888 28.339771071781566 22.46419161934089 60-61 21.321899246469528 28.661243769544072 27.884290867348255 22.13256611663815 62-63 21.170055531177383 28.6074104209655 27.96897699187208 22.25355705598503 64-65 21.090382195636472 28.354064438278893 28.190188511057862 22.36536485502677 66-67 21.057655256047568 28.43125004041665 27.879092019489764 22.632002684046018 68-69 21.64204976616416 28.56807987039092 27.684147323733427 22.105723039711492 70-71 21.42643132055567 28.319594479665273 27.868658045812538 22.38531615396652 72-73 21.134417125710957 28.48171436248824 28.157391732724257 22.22647677907654 74-75 21.21950406815453 28.47189553536656 27.96498402583383 22.343616370645083 76-77 21.380511070555926 28.563523014907467 27.648281457672013 22.407684456864594 78-79 21.59693035611437 28.65436884855778 27.65069666234277 22.09800413298507 80-81 21.36732821063352 28.432452961675597 27.841385305581717 22.358833522109165 82-83 21.36157080980101 28.453720666179628 27.857280799593266 22.327427724426098 84-85 21.570565594038925 28.325406246178233 27.64894266725577 22.455085492527072 86-87 21.791762370994537 28.405292328781773 27.55087060297153 22.252074697252162 88-89 21.62083316203038 28.256218341982674 27.653572313716662 22.469376182270285 90-91 21.704814769929484 28.132473911595145 27.57103006731038 22.591681251164992 92-93 21.957534779713182 28.294794205941404 27.308265396654214 22.4394056176912 94-95 22.031487437226442 28.03015191056002 27.413615116621216 22.524745535592324 96-97 22.09036985364181 27.948253416663576 27.442827796644544 22.518548933050074 98-99 22.2098142106537 27.96132617767178 27.301137753053716 22.527721858620804 100 22.41835244284747 28.05009504744669 27.098742750963865 22.43280975874197 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3968.0 1 3082.0 2 2547.5 3 2938.5 4 2691.0 5 2266.5 6 2041.0 7 1890.5 8 1900.0 9 1920.0 10 1755.0 11 1591.5 12 1575.5 13 1628.5 14 1795.0 15 2046.0 16 2296.5 17 2760.5 18 3472.5 19 4503.5 20 6004.5 21 8366.0 22 11724.5 23 16172.5 24 22060.5 25 29661.0 26 40174.0 27 52892.0 28 68096.5 29 84819.5 30 100821.5 31 115857.0 32 129761.5 33 143027.5 34 155403.5 35 166153.0 36 175344.0 37 183648.0 38 190507.5 39 195500.0 40 198190.5 41 199103.5 42 197858.5 43 195862.5 44 193653.0 45 190522.5 46 187629.5 47 183450.5 48 178865.5 49 175769.5 50 171002.5 51 164091.0 52 157007.0 53 150642.5 54 144877.5 55 137287.5 56 126406.5 57 113388.0 58 100976.0 59 89407.0 60 76968.0 61 64035.0 62 52737.5 63 42747.0 64 34101.5 65 26957.0 66 20952.0 67 16179.0 68 12334.5 69 9326.5 70 7011.0 71 5355.5 72 4105.0 73 3058.5 74 2243.0 75 1605.5 76 1061.5 77 729.0 78 529.5 79 401.0 80 285.0 81 187.5 82 148.0 83 110.0 84 71.0 85 55.0 86 51.0 87 42.5 88 37.0 89 35.5 90 27.0 91 19.0 92 13.5 93 13.5 94 12.5 95 9.0 96 10.0 97 11.5 98 13.5 99 20.0 100 35.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.02036780639799912 2 0.0029199229928529957 3 0.0016122274193666848 4 0.0025437365950007696 5 0.0022392047491203958 6 0.004370927670283012 7 11.62894263497792 8 3.5827275985926333E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.941000358451896E-4 18-19 0.0 20-21 8.956818996481583E-6 22-23 8.956818996481583E-6 24-25 0.0 26-27 2.5974775089796594E-4 28-29 0.0 30-31 8.956818996481583E-6 32-33 2.687045698944475E-5 34-35 9.85250089612974E-5 36-37 4.478409498240791E-5 38-39 5.37409139788895E-5 40-41 8.956818996481583E-6 42-43 5.37409139788895E-5 44-45 2.2392047491203956E-4 46-47 0.0012808251164968664 48-49 8.59854623662232E-4 50-51 4.926250448064871E-4 52-53 3.7618639785222646E-4 54-55 5.37409139788895E-4 56-57 0.0017734501613033533 58-59 3.8514321684870805E-4 60-61 0.0014868319534159426 62-63 0.0028572252598776248 64-65 0.001200213745528532 66-67 5.814856460705808 68-69 5.46794990415308 70-71 0.0031796707437509618 72-73 0.0015943137813737218 74-75 0.003600641236585596 76-77 0.006628046057396372 78-79 5.416690029036215 80-81 0.005759234614737658 82-83 0.005392005035881912 84-85 5.815268474379646 86-87 0.004648589059173942 88-89 0.0022123342921309508 90-91 0.0038245617114976357 92-93 0.004326143575300604 94-95 0.0048635527150895 96-97 0.0037976912545081907 98-99 0.0066638733333822964 100 0.006986318817255635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5582339.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.10222105315074 #Duplication Level Percentage of deduplicated Percentage of total 1 93.6294960210252 85.29855043602535 2 4.797218559437494 8.740745312843039 3 0.8968046722430787 2.4510269247656207 4 0.32508667491521015 1.1846447247823693 5 0.1316940391214146 0.5998809731710698 6 0.07447587576841118 0.40709506184284716 7 0.04873126717086273 0.3107668671800059 8 0.03063245742892937 0.22325479264732428 9 0.0164868128454197 0.13517867414748155 >10 0.04916915593749149 0.6211788765622561 >50 1.4038575764595938E-4 0.008810627148891963 >100 4.379527106939562E-5 0.005599284855123093 >500 2.0283077720983775E-5 0.013267444028658662 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.7913637992963163E-5 0.0 3 0.0 0.0 0.0 1.7913637992963163E-5 0.0 4 0.0 0.0 0.0 1.7913637992963163E-5 0.0 5 0.0 0.0 0.0 3.582727598592633E-5 0.0 6 0.0 0.0 0.0 3.582727598592633E-5 0.0 7 0.0 0.0 0.0 3.582727598592633E-5 0.0 8 0.0 0.0 0.0 3.582727598592633E-5 0.0 9 1.7913637992963163E-5 0.0 0.0 1.07481827957779E-4 0.0 10-11 2.6870456989444745E-5 0.0 0.0 1.1643864695426058E-4 0.0 12-13 3.582727598592633E-5 0.0 0.0 1.7017956093315007E-4 0.0 14-15 3.582727598592633E-5 1.7913637992963163E-5 1.7913637992963163E-5 1.8809319892611323E-4 1.7913637992963163E-5 16-17 3.582727598592633E-5 3.582727598592633E-5 1.7913637992963163E-5 2.507909319014843E-4 1.7913637992963163E-5 18-19 3.582727598592633E-5 5.37409139788895E-5 1.7913637992963163E-5 2.597477508979659E-4 1.7913637992963163E-5 20-21 3.582727598592633E-5 5.37409139788895E-5 1.7913637992963163E-5 3.8514321684870805E-4 1.7913637992963163E-5 22-23 3.582727598592633E-5 5.37409139788895E-5 1.7913637992963163E-5 0.0011016887365672347 1.7913637992963163E-5 24-25 3.582727598592633E-5 6.269773297537107E-5 1.7913637992963163E-5 0.0026691320609515115 1.7913637992963163E-5 26-27 3.582727598592633E-5 7.165455197185265E-5 1.7913637992963163E-5 0.005006861819033205 1.7913637992963163E-5 28-29 3.582727598592633E-5 7.165455197185265E-5 1.7913637992963163E-5 0.013094869372856073 1.7913637992963163E-5 30-31 3.582727598592633E-5 7.165455197185265E-5 1.7913637992963163E-5 0.03441209858448224 1.7913637992963163E-5 32-33 3.582727598592633E-5 7.165455197185265E-5 1.7913637992963163E-5 0.08331633030527169 1.7913637992963163E-5 34-35 3.582727598592633E-5 7.165455197185265E-5 1.7913637992963163E-5 0.1677343493471106 1.7913637992963163E-5 36-37 3.582727598592633E-5 7.165455197185265E-5 1.7913637992963163E-5 0.3011103410237178 1.7913637992963163E-5 38-39 3.582727598592633E-5 7.165455197185265E-5 1.7913637992963163E-5 0.4819037324676986 1.7913637992963163E-5 40-41 3.582727598592633E-5 8.956818996481583E-5 1.7913637992963163E-5 0.6910902401305259 1.7913637992963163E-5 42-43 3.582727598592633E-5 8.956818996481583E-5 1.7913637992963163E-5 0.9092425236088313 2.6870456989444745E-5 44-45 3.582727598592633E-5 8.956818996481583E-5 1.7913637992963163E-5 1.1784665890050747 3.582727598592633E-5 46-47 3.582727598592633E-5 9.852500896129741E-5 1.7913637992963163E-5 1.5000343046167566 3.582727598592633E-5 48-49 3.582727598592633E-5 1.2539546595074214E-4 1.7913637992963163E-5 1.8440925927286034 3.582727598592633E-5 50-51 3.582727598592633E-5 1.433091039437053E-4 1.7913637992963163E-5 2.197295793035858 3.582727598592633E-5 52-53 5.37409139788895E-5 1.6122274193666847E-4 1.7913637992963163E-5 2.5426259494452053 3.582727598592633E-5 54-55 5.37409139788895E-5 1.6122274193666847E-4 1.7913637992963163E-5 2.920630581553718 3.582727598592633E-5 56-57 5.37409139788895E-5 1.6122274193666847E-4 1.7913637992963163E-5 3.321493015741251 3.582727598592633E-5 58-59 5.37409139788895E-5 1.6122274193666847E-4 3.582727598592633E-5 3.7520741753591103 3.582727598592633E-5 60-61 5.37409139788895E-5 1.6122274193666847E-4 3.582727598592633E-5 4.186918780819294 3.582727598592633E-5 62-63 5.37409139788895E-5 1.6122274193666847E-4 3.582727598592633E-5 4.619568965625341 3.582727598592633E-5 64-65 5.37409139788895E-5 1.6122274193666847E-4 3.582727598592633E-5 5.084078555601872 3.582727598592633E-5 66-67 5.37409139788895E-5 1.6122274193666847E-4 3.582727598592633E-5 5.595118820265125 3.582727598592633E-5 68-69 5.37409139788895E-5 1.6122274193666847E-4 3.582727598592633E-5 6.079933518906681 3.582727598592633E-5 70-71 5.37409139788895E-5 1.7017956093315007E-4 3.582727598592633E-5 6.65128900269224 3.582727598592633E-5 72-73 5.37409139788895E-5 1.7913637992963166E-4 3.582727598592633E-5 7.265932792687796 3.582727598592633E-5 74-75 5.37409139788895E-5 1.7913637992963166E-4 3.582727598592633E-5 7.804130132548382 3.582727598592633E-5 76-77 5.37409139788895E-5 1.9705001792259482E-4 3.582727598592633E-5 8.342945492919725 3.582727598592633E-5 78-79 5.37409139788895E-5 1.9705001792259482E-4 3.582727598592633E-5 8.953504973452883 3.582727598592633E-5 80-81 5.37409139788895E-5 1.9705001792259482E-4 3.582727598592633E-5 9.65732285337741 3.582727598592633E-5 82-83 5.37409139788895E-5 2.14963655915558E-4 5.37409139788895E-5 10.365448963239245 3.582727598592633E-5 84-85 5.37409139788895E-5 2.3287729390852115E-4 5.37409139788895E-5 11.051371118808799 3.582727598592633E-5 86-87 5.37409139788895E-5 2.597477508979659E-4 5.37409139788895E-5 11.830694982873666 3.582727598592633E-5 88 5.37409139788895E-5 2.687045698944475E-4 5.37409139788895E-5 12.459221842313768 3.582727598592633E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5565 0.0 43.795723 1 GTATCAA 9265 0.0 36.363895 1 TCAACGC 9755 0.0 33.00612 4 ATCAACG 9765 0.0 32.972317 3 CAACGCA 10605 0.0 30.408298 5 TATCAAC 11010 0.0 29.61578 2 AACGCAG 11340 0.0 28.391676 6 ACGCAGA 13380 0.0 23.565577 7 GTACATG 16235 0.0 23.022924 1 CGCAGAG 13835 0.0 22.821327 8 GTGGTAT 3550 0.0 22.211725 1 TACATGG 16285 0.0 22.128702 2 ACATGGG 16815 0.0 20.73105 3 TGGTATC 3520 0.0 20.649841 2 TACCTGG 3725 0.0 19.925663 2 GCAGAGT 15865 0.0 19.074389 9 TCTGTCG 1570 0.0 18.151205 8 GTATAGG 1675 0.0 16.812712 1 CATGGGG 10535 0.0 16.71456 4 GTCTCGC 1570 0.0 16.632612 1 >>END_MODULE