FastQCFastQC Report
Wed 25 May 2016
SRR2049502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5582339
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220610.39519276776276036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215130.3853760941426166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117000.20958956451766902No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63550.1138411694452809No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.10251975023372818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120300.058.351351
GTATCAA219450.041.1268421
GTGGTAT44700.035.4945141
ATCAACG272100.032.9412733
TCAACGC275450.032.5235634
TATCAAC277850.032.2767872
CAACGCA285600.031.4647925
AACGCAG297300.030.447036
TGGTATC50400.029.9676272
ATAACGC5850.027.34623
ACGCAGA338900.026.611867
CGCAGAG340600.026.3412918
TATAACG6450.024.802592
GCAGAGT367050.023.8669249
GTACATG164750.022.261321
GAGTACT269450.021.69791212-13
TACATGG167400.021.1930642
CAGAGTA361300.021.17689110-11
AGAGTAC343750.020.58324410-11
TAACGCA7550.020.565584
Produced by FastQC (version 0.11.5)