##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049502_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5582339 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79802838917522 31.0 31.0 34.0 30.0 34.0 2 31.95945230126655 33.0 31.0 34.0 30.0 34.0 3 31.916808527751538 33.0 31.0 34.0 30.0 34.0 4 35.59653023580259 37.0 35.0 37.0 33.0 37.0 5 35.72542763884458 37.0 35.0 37.0 33.0 37.0 6 35.78137784179714 37.0 35.0 37.0 35.0 37.0 7 35.79346721866945 37.0 35.0 37.0 35.0 37.0 8 35.78390545611795 37.0 35.0 37.0 33.0 37.0 9 37.5228767009671 39.0 37.0 39.0 35.0 39.0 10-11 37.47763330030655 39.0 37.0 39.0 34.5 39.0 12-13 37.45896513988133 39.0 37.0 39.0 34.0 39.0 14-15 38.71773238780375 40.0 38.0 41.0 34.5 41.0 16-17 38.72295967693829 40.0 38.0 41.0 34.5 41.0 18-19 38.80582592708898 40.0 38.0 41.0 35.0 41.0 20-21 38.83040209489248 40.0 38.0 41.0 35.0 41.0 22-23 38.7948394929079 40.0 38.0 41.0 35.0 41.0 24-25 38.748924509959 40.0 38.0 41.0 34.0 41.0 26-27 38.65099459205183 40.0 38.0 41.0 34.0 41.0 28-29 38.55716125444907 40.0 38.0 41.0 34.0 41.0 30-31 38.4248604393248 40.0 38.0 41.0 34.0 41.0 32-33 38.25303452549191 40.0 38.0 41.0 34.0 41.0 34-35 38.136396589314984 40.0 38.0 41.0 34.0 41.0 36-37 37.98431168368671 40.0 37.0 41.0 33.0 41.0 38-39 37.881684630761406 40.0 37.0 41.0 33.0 41.0 40-41 37.7500660565401 40.0 37.0 41.0 33.0 41.0 42-43 37.59675827641424 40.0 37.0 41.0 33.0 41.0 44-45 37.394705337672974 40.0 37.0 41.0 32.5 41.0 46-47 37.1196746560895 40.0 37.0 41.0 31.5 41.0 48-49 36.85754716795236 40.0 37.0 41.0 31.0 41.0 50-51 36.636563992262026 40.0 36.0 41.0 30.5 41.0 52-53 36.52763456321803 40.0 36.0 41.0 30.0 41.0 54-55 36.39568969208068 39.5 35.5 41.0 30.0 41.0 56-57 36.2247794338538 39.0 35.0 41.0 30.0 41.0 58-59 36.05158232060074 39.0 35.0 41.0 30.0 41.0 60-61 35.85914972200721 39.0 35.0 41.0 29.0 41.0 62-63 35.654492767279095 39.0 35.0 41.0 29.0 41.0 64-65 35.37083389238812 38.0 35.0 40.0 29.0 41.0 66-67 35.11660954306071 37.5 35.0 40.0 28.0 41.0 68-69 34.80391490018789 37.0 34.0 40.0 28.0 41.0 70-71 34.506629568716626 36.5 34.0 39.5 28.0 41.0 72-73 34.18039123027104 36.0 34.0 39.0 28.0 41.0 74-75 33.844064916157905 35.5 34.0 39.0 27.0 40.5 76-77 33.550125135718204 35.0 34.0 37.5 27.0 40.0 78-79 33.22265192063757 35.0 34.0 37.0 26.5 39.0 80-81 32.911195378854636 35.0 33.5 37.0 26.0 39.0 82-83 32.61779327625929 35.0 33.0 36.0 26.0 39.0 84-85 32.341582712909414 35.0 33.0 36.0 26.0 37.0 86-87 32.063996382161676 35.0 33.0 35.5 25.5 37.0 88-89 31.849648417983932 35.0 33.0 35.0 25.0 36.5 90-91 31.69689004913532 35.0 33.0 35.0 25.0 36.0 92-93 31.53299916038779 35.0 33.0 35.0 25.0 36.0 94-95 31.343855326593385 35.0 33.0 35.0 24.5 36.0 96-97 31.16959691985743 35.0 33.0 35.0 24.0 35.5 98-99 30.982542532798526 35.0 33.0 35.0 23.0 35.0 100 30.864286815974452 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4228013676501128 1101 2 0.4364340074757891 1101 3 0.5217513698655978 1101 4 0.39741805238953987 1101 5 0.2955733643426939 1101 6 0.29596964163260964 1101 7 0.2934050763014966 1101 8 0.3203913548938857 1101 9 0.34258436084883215 1101 10-11 0.35307456663863235 1101 12-13 0.33348255652405356 1101 14-15 0.4724559199755234 1101 16-17 0.5146172292961424 1101 18-19 0.49880275528518325 1101 20-21 0.4641266123134997 1101 22-23 0.4541831608091087 1101 24-25 0.5099452381104328 1101 26-27 0.5004549971192276 1101 28-29 0.4775241946900337 1101 30-31 0.48263141221860906 1101 32-33 0.5111847505934435 1101 34-35 0.5260492433570079 1101 36-37 0.49638485189768744 1101 38-39 0.46268540377521106 1101 40-41 0.48592593302400644 1101 42-43 0.46132317672691414 1101 44-45 0.5381166271935314 1101 46-47 0.6042763481366151 1101 48-49 0.553749323355234 1101 50-51 0.5384666199672026 1101 52-53 0.5327244563759095 1101 54-55 0.4532448291396918 1101 56-57 0.4751504727850673 1101 58-59 0.43804710157270677 1101 60-61 0.5187898974791523 1101 62-63 0.4827352655985777 1101 64-65 0.4532356865877958 1101 66-67 0.503676221098587 1101 68-69 0.4241250358139794 1101 70-71 0.41662099472242886 1101 72-73 0.46409885112584703 1101 74-75 0.4255996016887664 1101 76-77 0.4047828922110597 1101 78-79 0.412125309944102 1101 80-81 0.3379032755927298 1101 82-83 0.3570010570467943 1101 84-85 0.38612301672615956 1101 86-87 0.47726647198305727 1101 88-89 0.4332994417408784 1101 90-91 0.4256522208335163 1101 92-93 0.420386856938574 1101 94-95 0.34800375315555954 1101 96-97 0.44733215145786787 1101 98-99 0.4863450130139704 1101 100 0.5123514383443784 1102 1 0.24767377796462497 1102 2 0.3303385251693882 1102 3 0.37431086616587095 1102 4 0.32128912593188375 1102 5 0.28638193757445407 1102 6 0.24290177435570115 1102 7 0.25029144072844645 1102 8 0.2700929416275457 1102 9 0.3462348918306404 1102 10-11 0.31891084434931827 1102 12-13 0.3137582098776548 1102 14-15 0.3744578481078662 1102 16-17 0.40950991481675914 1102 18-19 0.4194449525609514 1102 20-21 0.38352968639254215 1102 22-23 0.3504324961500842 1102 24-25 0.3406097480517474 1102 26-27 0.30626498663375656 1102 28-29 0.39658338537139315 1102 30-31 0.40372483221851496 1102 32-33 0.44712404424104335 1102 34-35 0.4585703260927758 1102 36-37 0.45987979055027495 1102 38-39 0.4667210642847692 1102 40-41 0.4780340078236165 1102 42-43 0.44808938074120874 1102 44-45 0.5029597676639384 1102 46-47 0.5499676183683349 1102 48-49 0.6204269227310704 1102 50-51 0.586587933283738 1102 52-53 0.5387565786632251 1102 54-55 0.5681684741049295 1102 56-57 0.4902040679070936 1102 58-59 0.5635267741877641 1102 60-61 0.5193285818766782 1102 62-63 0.5397406474731383 1102 64-65 0.5482565829705592 1102 66-67 0.5906759875361587 1102 68-69 0.5595778239032896 1102 70-71 0.5654265962450609 1102 72-73 0.5761054505066596 1102 74-75 0.5342145765186856 1102 76-77 0.5244664896687112 1102 78-79 0.5219684752615734 1102 80-81 0.4478560164726133 1102 82-83 0.47755653806115816 1102 84-85 0.4883965232932397 1102 86-87 0.47714149123953575 1102 88-89 0.5267947282523977 1102 90-91 0.5300205835609297 1102 92-93 0.5202770329055006 1102 94-95 0.5217232595934362 1102 96-97 0.6195993468701069 1102 98-99 0.6350102286119643 1102 100 0.7128568276730611 1103 1 0.28881142356580725 1103 2 0.31770660762337144 1103 3 0.3691944583280886 1103 4 0.3472737277905722 1103 5 0.25714851352545764 1103 6 0.2797198732427617 1103 7 0.2944679974365414 1103 8 0.29418561018734835 1103 9 0.3231242981865847 1103 10-11 0.28541068510423884 1103 12-13 0.2974126079979911 1103 14-15 0.40383459273151345 1103 16-17 0.3511239606780734 1103 18-19 0.4089444716471604 1103 20-21 0.3493658470412697 1103 22-23 0.3747812282604457 1103 24-25 0.39297018292749897 1103 26-27 0.4082008315258676 1103 28-29 0.455975444222787 1103 30-31 0.41892189880330477 1103 32-33 0.42603451226901257 1103 34-35 0.4336044795541696 1103 36-37 0.4945295657953892 1103 38-39 0.5282148403766485 1103 40-41 0.5435221973055562 1103 42-43 0.49756288661865966 1103 44-45 0.5405580403156733 1103 46-47 0.5622162670101076 1103 48-49 0.5618253838069336 1103 50-51 0.5527974457776423 1103 52-53 0.5389158396389035 1103 54-55 0.5229932504784287 1103 56-57 0.5572295604702546 1103 58-59 0.5123221375812221 1103 60-61 0.4065605908376355 1103 62-63 0.40684323563483815 1103 64-65 0.556292182034035 1103 66-67 0.4956984375964595 1103 68-69 0.531301157662547 1103 70-71 0.5253278579365386 1103 72-73 0.5574099983046636 1103 74-75 0.5654187617718947 1103 76-77 0.5300389868668205 1103 78-79 0.47177819169090185 1103 80-81 0.4921230027737238 1103 82-83 0.5231300492149842 1103 84-85 0.5682547856337763 1103 86-87 0.5428608562680424 1103 88-89 0.5597524847593576 1103 90-91 0.45292490555511833 1103 92-93 0.5381918027756889 1103 94-95 0.5103066585637137 1103 96-97 0.537310372033371 1103 98-99 0.5278010193512124 1103 100 0.5339584222217297 1104 1 0.2502396107163882 1104 2 0.271253043114811 1104 3 0.3645476067956892 1104 4 0.2720542540232458 1104 5 0.19748134029234166 1104 6 0.20129492063204424 1104 7 0.1751941147097824 1104 8 0.21751116389290104 1104 9 0.31321013628186023 1104 10-11 0.28098859116147423 1104 12-13 0.2865523717030598 1104 14-15 0.3197433711418185 1104 16-17 0.3108935079685793 1104 18-19 0.29363033339954825 1104 20-21 0.279966098263003 1104 22-23 0.2657600923707406 1104 24-25 0.2532401772413877 1104 26-27 0.3225654578965944 1104 28-29 0.3604106126236033 1104 30-31 0.38020243914618845 1104 32-33 0.3555300543441504 1104 34-35 0.2880328030033539 1104 36-37 0.32393402197899235 1104 38-39 0.3498662930375076 1104 40-41 0.40132562230319735 1104 42-43 0.4032311370310069 1104 44-45 0.43273729278691064 1104 46-47 0.46734664832795403 1104 48-49 0.4564932415352132 1104 50-51 0.4911989604333442 1104 52-53 0.4934444806578142 1104 54-55 0.480420383266285 1104 56-57 0.5014415834476651 1104 58-59 0.4687400903282395 1104 60-61 0.44559566881304136 1104 62-63 0.4881577035209119 1104 64-65 0.4970011659129696 1104 66-67 0.48338142747132906 1104 68-69 0.5032417344758144 1104 70-71 0.48743498623314707 1104 72-73 0.4920107634982003 1104 74-75 0.516154534029539 1104 76-77 0.4375141844247281 1104 78-79 0.4045376048503755 1104 80-81 0.4162098471969813 1104 82-83 0.4150535917056217 1104 84-85 0.41139935641628256 1104 86-87 0.44620737714124203 1104 88-89 0.4459547434733153 1104 90-91 0.42250433431388146 1104 92-93 0.443327392861562 1104 94-95 0.515796039581943 1104 96-97 0.5145358519455421 1104 98-99 0.5710436560746182 1104 100 0.6149199698052534 1105 1 0.22735463484687912 1105 2 0.2763613479197389 1105 3 0.35624677336966215 1105 4 0.2585524617378354 1105 5 0.20321278282487754 1105 6 0.23799427211122293 1105 7 0.22517852367576552 1105 8 0.2112895858730468 1105 9 0.25607475962852533 1105 10-11 0.2405543776266228 1105 12-13 0.24912361596542354 1105 14-15 0.3125219668033523 1105 16-17 0.27419022095843815 1105 18-19 0.3148584795112157 1105 20-21 0.30243951256418455 1105 22-23 0.32759068697262705 1105 24-25 0.3439912190059289 1105 26-27 0.389848971676777 1105 28-29 0.39183402870189354 1105 30-31 0.3481421433583023 1105 32-33 0.34204317678579343 1105 34-35 0.3302842749423007 1105 36-37 0.4235139562103001 1105 38-39 0.45541998195385247 1105 40-41 0.47134860378950805 1105 42-43 0.4355983178972167 1105 44-45 0.5023301790161128 1105 46-47 0.4613971687461671 1105 48-49 0.4831974235728751 1105 50-51 0.5592751923156953 1105 52-53 0.5412221215753661 1105 54-55 0.46029728676462156 1105 56-57 0.4700049146307279 1105 58-59 0.41094903407058325 1105 60-61 0.4202259843159979 1105 62-63 0.3885491053858203 1105 64-65 0.4697290515025543 1105 66-67 0.4508258087778074 1105 68-69 0.43860837271360964 1105 70-71 0.4547234140309797 1105 72-73 0.41903429192978336 1105 74-75 0.4582225541063636 1105 76-77 0.3812739935737852 1105 78-79 0.3351082769858209 1105 80-81 0.35482528423445103 1105 82-83 0.3670262640117343 1105 84-85 0.4083062521140661 1105 86-87 0.43560766121894545 1105 88-89 0.4204761951388747 1105 90-91 0.3579347972229776 1105 92-93 0.3358032476236339 1105 94-95 0.3790736504973644 1105 96-97 0.4261767280537292 1105 98-99 0.49975333289053836 1105 100 0.5191968343367925 1106 1 0.17733079727681655 1106 2 0.19297429125342802 1106 3 0.34018444238427037 1106 4 0.20991375593582262 1106 5 0.17006934195151047 1106 6 0.17389326741615463 1106 7 0.22136004627981265 1106 8 0.19268454352948794 1106 9 0.18881356544379457 1106 10-11 0.22490373827324817 1106 12-13 0.19984877872685303 1106 14-15 0.3185827836121149 1106 16-17 0.33125819690987157 1106 18-19 0.28742690674980764 1106 20-21 0.25374979454368685 1106 22-23 0.23465850451403014 1106 24-25 0.2728482113246997 1106 26-27 0.26516141104742985 1106 28-29 0.24150903202118457 1106 30-31 0.23604358177864526 1106 32-33 0.27182270169831924 1106 34-35 0.2687106500262004 1106 36-37 0.23163300368280204 1106 38-39 0.2522934033053872 1106 40-41 0.22158913930977775 1106 42-43 0.18179628513777146 1106 44-45 0.22781733907322632 1106 46-47 0.2055422256670809 1106 48-49 0.15394203210825452 1106 50-51 0.16454412503303217 1106 52-53 0.14882394562715007 1106 54-55 0.07814463925351589 1106 56-57 0.13353391813348736 1106 58-59 0.08915605326531306 1106 60-61 0.1055653560444938 1106 62-63 0.12622054429786544 1106 64-65 0.09358112829510645 1106 66-67 0.09596468420416926 1106 68-69 0.09246801369003066 1106 70-71 0.07700256988999854 1106 72-73 0.13977527796959066 1106 74-75 0.1425763641908162 1106 76-77 0.11780996749494221 1106 78-79 0.11464251965975336 1106 80-81 0.05625608694165152 1106 82-83 0.09516324015061883 1106 84-85 0.10699476775355521 1106 86-87 0.16619671305338102 1106 88-89 0.15914622439700565 1106 90-91 0.09884601445532226 1106 92-93 0.0948722954317951 1106 94-95 0.13695286447821076 1106 96-97 0.1935376828774089 1106 98-99 0.23031971185248423 1106 100 0.24077386248664823 1107 1 0.1694944239670022 1107 2 0.16060524964650824 1107 3 0.26792517012500383 1107 4 0.24434355329864843 1107 5 0.1473724135963863 1107 6 0.1578294080497571 1107 7 0.17023265693071465 1107 8 0.16213043583912423 1107 9 0.17023493312382243 1107 10-11 0.1386337965760589 1107 12-13 0.09451551213491882 1107 14-15 0.1553623310729506 1107 16-17 0.15816442064058123 1107 18-19 0.1280483138261772 1107 20-21 0.19582384724129298 1107 22-23 0.1923718453105039 1107 24-25 0.184139769671674 1107 26-27 0.14937013087970286 1107 28-29 0.20470066686408472 1107 30-31 0.2234593612844975 1107 32-33 0.21384490821243674 1107 34-35 0.17598484182695273 1107 36-37 0.21871173908716912 1107 38-39 0.20834481553462325 1107 40-41 0.1980178444913676 1107 42-43 0.2163803443176775 1107 44-45 0.145064953203061 1107 46-47 0.23636306255027506 1107 48-49 0.21106391434560834 1107 50-51 0.3263615286518231 1107 52-53 0.2870498428599717 1107 54-55 0.2722782520360809 1107 56-57 0.2893548293239192 1107 58-59 0.2885095195356371 1107 60-61 0.29639478959909127 1107 62-63 0.25550232975747633 1107 64-65 0.3336320854938677 1107 66-67 0.2918717201804881 1107 68-69 0.31996372346056745 1107 70-71 0.3581149005404356 1107 72-73 0.2684301535644167 1107 74-75 0.2638044614581787 1107 76-77 0.32436664864746945 1107 78-79 0.29999423336019504 1107 80-81 0.3142826665040275 1107 82-83 0.36090978060847334 1107 84-85 0.31229841953526005 1107 86-87 0.39922117823719816 1107 88-89 0.3035454855399138 1107 90-91 0.24064362397810513 1107 92-93 0.2264617933607873 1107 94-95 0.3079609785579187 1107 96-97 0.3343409400201587 1107 98-99 0.3809285447938784 1107 100 0.3674797471601998 1108 1 0.11892165238986507 1108 2 0.11941067232910285 1108 3 0.17607977501088357 1108 4 0.14312732752535595 1108 5 0.11810214111402928 1108 6 0.1684931598254309 1108 7 0.0889818010445893 1108 8 0.11126845752571768 1108 9 0.1474500856125971 1108 10-11 0.13642561699320055 1108 12-13 0.10477312028383068 1108 14-15 0.08316402215825747 1108 16-17 0.15598982093040803 1108 18-19 0.1353613823365336 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0.3368776670700129 2206 36-37 0.31266759496519114 2206 38-39 0.3019154479482751 2206 40-41 0.016249544437989982 2206 42-43 0.2914087714292677 2206 44-45 0.3410610627268653 2206 46-47 0.34533915703418216 2206 48-49 0.40684418641151865 2206 50-51 0.40531607950346427 2206 52-53 0.3766234308374692 2206 54-55 0.4051622694207495 2206 56-57 0.3832602223989383 2206 58-59 0.4357779228144665 2206 60-61 0.36249239701507463 2206 62-63 0.375091952444393 2206 64-65 0.41476070868034043 2206 66-67 0.4109748996312419 2206 68-69 0.44765953892555643 2206 70-71 0.4227937196413123 2206 72-73 0.4360658201494374 2206 74-75 0.15387230143627306 2206 76-77 0.23834829484197684 2206 78-79 0.31181619460091525 2206 80-81 0.31462392555497587 2206 82-83 0.299423435784135 2206 84-85 0.3430612609414041 2206 86-87 0.38946233534632313 2206 88-89 0.3763306043882366 2206 90-91 0.3449649460768036 2206 92-93 0.3796134588204829 2206 94-95 0.40374539568713885 2206 96-97 0.41077884911116413 2206 98-99 0.4797891604882132 2206 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0.14028930131409822 2208 82-83 0.1980452089742002 2208 84-85 0.1836437820786614 2208 86-87 0.2039545416936619 2208 88-89 0.13193452627354674 2208 90-91 0.11757723475309234 2208 92-93 0.10380932820518751 2208 94-95 0.004480052302930915 2208 96-97 0.12634529893758994 2208 98-99 0.24193976314344567 2208 100 0.21385715023239626 2209 1 0.18349047709019217 2209 2 0.17813033026162373 2209 3 0.28054642110058126 2209 4 0.16579461503889092 2209 5 0.1243569435799543 2209 6 0.14864139658507014 2209 7 0.1698137003862712 2209 8 0.14911599065109016 2209 9 0.13029836176190202 2209 10-11 0.1443640550465375 2209 12-13 0.1308312785018515 2209 14-15 0.16127801782256768 2209 16-17 0.16035323394336132 2209 18-19 0.14738923973031604 2209 20-21 0.15584616687085173 2209 22-23 0.20144090071998733 2209 24-25 0.20033465263652772 2209 26-27 0.17924694933856244 2209 28-29 0.19594312838859906 2209 30-31 0.2672644087961089 2209 32-33 0.2626652933882738 2209 34-35 0.22937770618884912 2209 36-37 0.2520977708305878 2209 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-0.6252777842079382 2215 26-27 -0.6284653170709973 2215 28-29 -0.5899804874870114 2215 30-31 -0.5411439563010134 2215 32-33 -0.6048759239629646 2215 34-35 -0.6932018370418902 2215 36-37 -0.7302107882958495 2215 38-39 -0.7821827687226488 2215 40-41 -0.7058548280719492 2215 42-43 -0.6814869905936405 2215 44-45 -0.8200109190766085 2215 46-47 -0.7538219345269539 2215 48-49 -0.7652641500128681 2215 50-51 -0.6563168562532553 2215 52-53 -0.8114561895492542 2215 54-55 -0.7456014447622792 2215 56-57 -0.6571771342995518 2215 58-59 -0.706959585329578 2215 60-61 -0.8841487740742053 2215 62-63 -0.7796829308483524 2215 64-65 -0.653652899125639 2215 66-67 -0.5983234586835593 2215 68-69 -0.5744408456462153 2215 70-71 -0.7094462860090687 2215 72-73 -0.5828128629276819 2215 74-75 -0.6671315202577262 2215 76-77 -0.6169135493343845 2215 78-79 -0.6283536579318607 2215 80-81 -0.6405849463101703 2215 82-83 -0.6370926726032202 2215 84-85 -0.6289642897304013 2215 86-87 -0.6031395588762578 2215 88-89 -0.6198329750697482 2215 90-91 -0.6870472425997569 2215 92-93 -0.8236740436539627 2215 94-95 -0.844460969062176 2215 96-97 -0.8606081750028878 2215 98-99 -0.8119875668655858 2215 100 -0.8663247433671444 2216 1 -0.5709460484155358 2216 2 -1.0041391553630028 2216 3 -0.6758344941118004 2216 4 -1.101610259365529 2216 5 -0.5978937619956923 2216 6 -0.7816578919123387 2216 7 -0.5460795034658972 2216 8 -0.48797728325559575 2216 9 -0.6309812493808451 2216 10-11 -0.8426755758269451 2216 12-13 -0.6393743358441739 2216 14-15 -0.8942371339791748 2216 16-17 -0.8170031650691882 2216 18-19 -0.8789708108407552 2216 20-21 -0.6859427510136769 2216 22-23 -0.7060684724952608 2216 24-25 -0.7916722940506844 2216 26-27 -0.8922539889127066 2216 28-29 -1.1328231892295335 2216 30-31 -1.2371599992778215 2216 32-33 -0.9907776642817652 2216 34-35 -1.0239652395681773 2216 36-37 -0.940466027263156 2216 38-39 -1.0319676463632135 2216 40-41 -0.9381838827892892 2216 42-43 -1.5220710106778341 2216 44-45 -1.2271380617072225 2216 46-47 -1.1256837099326091 2216 48-49 -1.0812232020629438 2216 50-51 -1.2868319560647166 2216 52-53 -1.1876098714229713 2216 54-55 -1.3810887285285602 2216 56-57 -1.42857825729876 2216 58-59 -1.2017071098642447 2216 60-61 -1.3536156175499983 2216 62-63 -1.304856520477017 2216 64-65 -1.264606976413873 2216 66-67 -1.160656502834513 2216 68-69 -1.50786439379943 2216 70-71 -1.2620120921862963 2216 72-73 -1.1327941531485521 2216 74-75 -1.1636980752844153 2216 76-77 -1.3078001912166073 2216 78-79 -1.4172864168417476 2216 80-81 -1.187807397678494 2216 82-83 -1.2286479971795075 2216 84-85 -1.2642108211458734 2216 86-87 -1.407575621156333 2216 88-89 -1.22292293003796 2216 90-91 -1.1555949696079288 2216 92-93 -1.2244365502189964 2216 94-95 -1.334478482698664 2216 96-97 -1.4455280242797492 2216 98-99 -1.4674605152518012 2216 100 -1.5132150932915707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 348.0 3 44.0 4 48.0 5 144.0 6 411.0 7 1030.0 8 2297.0 9 3920.0 10 5570.0 11 7875.0 12 11582.0 13 16243.0 14 18230.0 15 23309.0 16 29446.0 17 41614.0 18 43182.0 19 23944.0 20 17160.0 21 16199.0 22 16820.0 23 18178.0 24 20882.0 25 25611.0 26 31759.0 27 41098.0 28 52901.0 29 69051.0 30 89816.0 31 114912.0 32 148231.0 33 192764.0 34 262298.0 35 380845.0 36 606273.0 37 1109815.0 38 1532749.0 39 603748.0 40 1992.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12136904451422 20.50743618464603 14.624953552413581 22.74624121842617 2 16.056289888231 20.92688394442666 39.20669499779393 23.810131169548416 3 17.991365268214633 25.647976591890963 30.997920405765395 25.36273773412901 4 12.77061940953939 17.95412972872731 37.94429774587176 31.330953115861544 5 12.39426486955833 37.12448454711713 35.15596911411879 15.325281469205748 6 29.621965745163347 38.155901801774526 17.72714399058869 14.494988462473435 7 25.824802112519503 33.19225149171342 22.607369419879372 18.375576975887707 8 24.872333980433652 35.093210928250684 20.920155511874146 19.11429957944152 9 24.58555425283506 16.735219222780433 22.260022015876867 36.41920450850764 10-11 23.453654462761936 27.03339944062874 28.627077287853712 20.885868808755614 12-13 24.657484431924797 24.649691997303997 29.05213093977209 21.640692630999112 14-15 21.95489989228526 26.62616929074687 27.228936554708184 24.189994262259695 16-17 20.35476526954024 29.797079682907114 27.85248262421899 21.99567242333366 18-19 20.130674615067267 27.69594429861748 31.365803832407884 20.80757725390737 20-21 21.48333341991592 27.034536956641293 31.09971465366041 20.38241496978238 22-23 21.067253350253363 27.075084476238366 31.174978803687846 20.682683369820428 24-25 20.82420110996484 27.058514361094876 31.312501802559822 20.804782726380466 26-27 20.677139098861606 26.902056646864338 31.362606248026143 21.05819800624792 28-29 20.719653162623192 26.928720419151293 31.4729177504439 20.878708667781616 30-31 21.142124064032792 27.377265788980115 30.936122158375888 20.544487988611202 32-33 20.602788544371816 27.174567864832287 31.368338612183887 20.854304978612014 34-35 20.68242362206953 26.742795448287893 31.382839702139194 21.19194122750338 36-37 21.266542572925076 27.184438279366407 30.73555726372046 20.813461883988055 38-39 21.14146059563921 27.145646296292647 30.859385286346818 20.853507821721326 40-41 20.842383452527695 26.97255576918564 31.004826113211685 21.18023466507498 42-43 20.83914108405097 27.026323553621516 30.810015300038206 21.324520062289302 44-45 21.152155037521013 27.148709528389443 30.5447770190954 21.154358414994146 46-47 21.067468313909277 27.547744771501698 30.03391589081208 21.350871023776953 48-49 21.05872645856871 27.276299414994327 30.00848748168107 21.656486644755898 50-51 21.141559120648175 27.24644633727905 29.738949927619945 21.87304461445283 52-53 21.041592063828443 27.41976078486097 29.649444435388105 21.889202715922483 54-55 21.191950184322376 27.795391859935414 29.39189110514428 21.62076685059793 56-57 20.8803066241588 27.504465422110698 29.453917076694914 22.161310877035596 58-59 20.639025567740337 27.086748314211235 29.830164926726354 22.444061191322078 60-61 21.027305937529054 27.416231798176355 29.396727787402376 22.15973447689221 62-63 20.81215418841457 27.322821132862053 29.52688828105925 22.338136397664133 64-65 20.66342620897799 27.108717331570155 29.749868289976654 22.477988169475196 66-67 20.685755558736222 27.16911316206343 29.39877889895257 22.746352380247778 68-69 21.124164978157005 27.358567797477008 29.096575826011282 22.420691398354702 70-71 21.076890887493576 27.097288430530643 29.136057484147777 22.689763197828007 72-73 20.78718257705238 27.233503733829135 29.2569028860483 22.722410803070183 74-75 20.89324027078972 27.199772353488385 29.010814284119974 22.896173091601927 76-77 20.920486914177015 27.395245971267602 28.680164354045857 23.00410276050953 78-79 21.02207652841828 27.528461898043854 28.65521332797954 22.79424824555833 80-81 20.838164060820592 27.370054027716073 28.663002120981957 23.128779790481378 82-83 20.667430447146568 27.371892633519096 28.718787951854065 23.24188896748027 84-85 20.770970734668747 27.3023368878171 28.49989941492267 23.426792962591485 86-87 20.923989030404638 27.454244537997425 28.38150818142718 23.24025825017076 88-89 20.666182102194735 27.38100068385393 28.38182784705139 23.570989366899948 90-91 20.581364354049757 27.21988687885748 28.305427443073093 23.893321324019666 92-93 20.693226806155167 27.3609981548824 28.0855292621321 23.86024577683034 94-95 20.588107263352835 27.15958553269639 28.265644363665288 23.98666284028549 96-97 20.530983565400057 27.17114879364625 28.266666380048434 24.031201260905263 98-99 20.535060757696865 27.21235260208178 28.115576305125202 24.137010335096154 100 20.682703369484646 27.23234134881853 27.946565900157605 24.13838938153922 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1173.0 1 986.5 2 2623.5 3 4020.0 4 2316.5 5 1114.5 6 2139.5 7 4790.5 8 7995.0 9 9373.5 10 7283.5 11 4654.0 12 4103.0 13 4509.0 14 5139.5 15 5732.0 16 6288.5 17 6995.5 18 7875.0 19 8820.5 20 10009.0 21 11800.0 22 14536.0 23 18320.5 24 23364.5 25 30055.0 26 39067.0 27 50280.0 28 64238.0 29 80433.0 30 95890.0 31 110088.0 32 123535.0 33 136347.5 34 148183.5 35 158427.0 36 166887.5 37 174984.5 38 183637.5 39 189910.0 40 193153.5 41 195211.0 42 195086.0 43 194619.0 44 193087.0 45 190037.5 46 187603.5 47 183575.0 48 179543.0 49 176964.0 50 173002.0 51 165702.5 52 156549.0 53 149224.0 54 144191.5 55 138115.5 56 128909.5 57 117068.0 58 104662.0 59 93460.0 60 81177.0 61 67527.5 62 55520.0 63 44725.0 64 35161.0 65 27669.0 66 21466.5 67 16375.0 68 12364.0 69 9206.0 70 6826.0 71 5038.0 72 3749.0 73 2761.5 74 2022.0 75 1377.5 76 852.5 77 515.0 78 325.5 79 239.0 80 151.5 81 73.5 82 42.0 83 25.5 84 12.5 85 7.5 86 6.5 87 7.0 88 4.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06216032383558218 2 3.941000358451896E-4 3 0.0 4 0.11926900175714876 5 0.0020063274552118747 6 0.00214963655915558 7 0.0 8 0.0 9 7.165455197185267E-5 10-11 0.0 12-13 2.687045698944475E-5 14-15 3.5827275985926333E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.970500179225948E-4 30-31 2.14963655915558E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.060068369190764E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.3435228494722374E-4 80-81 3.4035912186630013E-4 82-83 3.493159408627817E-4 84-85 0.0 86-87 0.0 88-89 1.1643864695426056E-4 90-91 8.956818996481582E-5 92-93 6.986318817255635E-4 94-95 7.344591577114897E-4 96-97 8.956818996481583E-6 98-99 1.7913637992963167E-5 100 0.001092731917570753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5582339.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.9092248400437 #Duplication Level Percentage of deduplicated Percentage of total 1 78.78619226669925 51.13950668130094 2 13.29092737090647 17.254075861017178 3 3.7505694683074546 7.30339670689715 4 1.509846275305495 3.920118054308276 5 0.7623020840390505 2.474021868446231 6 0.45767294566511596 1.782431768002927 7 0.28884801601364385 1.3124230571221105 8 0.2057615181511116 1.068465651607939 9 0.16351618935242454 0.9552338189697316 >10 0.7399518955186954 8.195922329308369 >50 0.031998680068354185 1.415586992520638 >100 0.011365364358012971 1.2732430552649965 >500 4.96388439120848E-4 0.2186672982825896 >1k 4.1365288180452456E-4 0.4813110905746669 >5k 5.515371754336579E-5 0.2161952973377823 >10k+ 8.273057631504868E-5 0.9894004690386305 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22061 0.39519276776276036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21513 0.3853760941426166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11700 0.20958956451766902 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6355 0.1138411694452809 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5723 0.10251975023372818 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.7913637992963163E-5 0.0 0.0 0.0 3 0.0 1.7913637992963163E-5 0.0 3.582727598592633E-5 0.0 4 0.0 1.7913637992963163E-5 0.0 3.582727598592633E-5 0.0 5 0.0 1.7913637992963163E-5 0.0 5.37409139788895E-5 0.0 6 0.0 1.7913637992963163E-5 0.0 5.37409139788895E-5 0.0 7 0.0 1.7913637992963163E-5 0.0 5.37409139788895E-5 0.0 8 0.0 1.7913637992963163E-5 0.0 5.37409139788895E-5 0.0 9 0.0 1.7913637992963163E-5 0.0 1.2539546595074214E-4 0.0 10-11 0.0 1.7913637992963163E-5 0.0 1.522659229401869E-4 0.0 12-13 0.0 1.7913637992963163E-5 0.0 2.2392047491203956E-4 0.0 14-15 0.0 1.7913637992963163E-5 0.0 2.5079093190148434E-4 0.0 16-17 0.0 1.7913637992963163E-5 0.0 2.687045698944475E-4 0.0 18-19 0.0 1.7913637992963163E-5 0.0 3.134886648768554E-4 0.0 20-21 0.0 1.7913637992963163E-5 0.0 4.388841308275975E-4 0.0 22-23 0.0 1.7913637992963163E-5 0.0 0.0011196023745601979 0.0 24-25 0.0 4.478409498240791E-5 0.0 0.002696002517940956 0.0 26-27 0.0 5.37409139788895E-5 0.0 0.005042689095019131 0.0 28-29 0.0 7.165455197185265E-5 0.0 0.013157567105831445 0.0 30-31 0.0 7.165455197185265E-5 0.0 0.0344300122224752 0.0 32-33 0.0 8.956818996481583E-5 0.0 0.08387165308305353 0.0 34-35 1.7913637992963163E-5 8.956818996481583E-5 0.0 0.16917639720554412 0.0 36-37 1.7913637992963163E-5 1.0748182795777898E-4 0.0 0.3038153003606553 0.0 38-39 1.7913637992963163E-5 1.433091039437053E-4 0.0 0.48617613512902025 0.0 40-41 1.7913637992963163E-5 1.6122274193666847E-4 0.0 0.6969390429352285 0.0 42-43 1.7913637992963163E-5 1.7913637992963166E-4 0.0 0.9164169356250131 0.0 44-45 1.7913637992963163E-5 1.8809319892611323E-4 0.0 1.187011394327718 0.0 46-47 1.7913637992963163E-5 1.9705001792259482E-4 0.0 1.5105585669376222 0.0 48-49 1.7913637992963163E-5 1.9705001792259482E-4 0.0 1.8563813483917762 0.0 50-51 1.7913637992963163E-5 1.9705001792259482E-4 0.0 2.210963898824489 0.0 52-53 1.7913637992963163E-5 1.9705001792259482E-4 0.0 2.55788836901521 0.0 54-55 1.7913637992963163E-5 1.9705001792259482E-4 0.0 2.9520242321363854 0.0 56-57 1.7913637992963163E-5 1.9705001792259482E-4 0.0 3.41316605817024 0.0 58-59 1.7913637992963163E-5 1.9705001792259482E-4 0.0 3.909123039643418 0.0 60-61 3.582727598592633E-5 1.9705001792259482E-4 0.0 4.406450414423059 0.0 62-63 3.582727598592633E-5 1.9705001792259482E-4 0.0 4.903840486935674 1.7913637992963163E-5 64-65 3.582727598592633E-5 1.9705001792259482E-4 0.0 5.438392401464691 1.7913637992963163E-5 66-67 3.582727598592633E-5 2.14963655915558E-4 0.0 6.043103079193148 1.7913637992963163E-5 68-69 3.582727598592633E-5 2.14963655915558E-4 0.0 6.684742721644099 1.7913637992963163E-5 70-71 3.582727598592633E-5 2.14963655915558E-4 0.0 7.359522594382033 1.7913637992963163E-5 72-73 3.582727598592633E-5 2.3287729390852115E-4 0.0 8.0601787172008 1.7913637992963163E-5 74-75 5.37409139788895E-5 2.597477508979659E-4 0.0 8.747256302420903 1.7913637992963163E-5 76-77 5.37409139788895E-5 2.687045698944475E-4 0.0 9.461544703752317 1.7913637992963163E-5 78-79 6.269773297537107E-5 2.687045698944475E-4 0.0 10.23837857213616 1.7913637992963163E-5 80-81 7.165455197185265E-5 2.687045698944475E-4 0.0 11.04711662978547 1.7913637992963163E-5 82-83 7.165455197185265E-5 2.77661388890929E-4 0.0 11.857610223958094 1.7913637992963163E-5 84-85 7.165455197185265E-5 2.866182078874106E-4 0.0 12.645460979707611 1.7913637992963163E-5 86-87 7.165455197185265E-5 3.045318458803738E-4 0.0 13.454628964668753 1.7913637992963163E-5 88 7.165455197185265E-5 3.2244548387333694E-4 0.0 14.090491458866973 1.7913637992963163E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12030 0.0 58.35135 1 GTATCAA 21945 0.0 41.126842 1 GTGGTAT 4470 0.0 35.494514 1 ATCAACG 27210 0.0 32.941273 3 TCAACGC 27545 0.0 32.523563 4 TATCAAC 27785 0.0 32.276787 2 CAACGCA 28560 0.0 31.464792 5 AACGCAG 29730 0.0 30.44703 6 TGGTATC 5040 0.0 29.967627 2 ATAACGC 585 0.0 27.3462 3 ACGCAGA 33890 0.0 26.61186 7 CGCAGAG 34060 0.0 26.341291 8 TATAACG 645 0.0 24.80259 2 GCAGAGT 36705 0.0 23.866924 9 GTACATG 16475 0.0 22.26132 1 GAGTACT 26945 0.0 21.697912 12-13 TACATGG 16740 0.0 21.193064 2 CAGAGTA 36130 0.0 21.176891 10-11 AGAGTAC 34375 0.0 20.583244 10-11 TAACGCA 755 0.0 20.56558 4 >>END_MODULE