FastQCFastQC Report
Wed 25 May 2016
SRR2049501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742901
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73200.043.5946541
GTATCAA113950.038.4782831
TCAACGC121800.034.264624
ATCAACG123500.033.875893
CAACGCA136250.030.6306845
TATCAAC141650.029.969092
AACGCAG148800.028.2871136
ACGCAGA171950.023.9125967
GTACATG228100.023.6685471
CGCAGAG174200.022.87428
TACATGG229450.022.540782
ACATGGG232350.021.3338013
GCAGAGT201150.018.9325989
CATGGGG162500.017.2688244
GAGTACA172400.017.1730271
GTGGTAT45050.017.168741
AGAGTAC176450.016.46909310-11
CAGAGTA192950.016.31483510-11
GTATAGG23150.015.7095461
CCGATCG2651.264809E-515.3608369
Produced by FastQC (version 0.11.5)