##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049501_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7742901 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.18039324020803 31.0 30.0 34.0 26.0 34.0 2 30.444750488221406 31.0 31.0 34.0 27.0 34.0 3 30.498702747200305 31.0 31.0 34.0 27.0 34.0 4 34.083718880042504 37.0 35.0 37.0 30.0 37.0 5 34.10984668407875 37.0 35.0 37.0 30.0 37.0 6 34.06496105271138 37.0 35.0 37.0 30.0 37.0 7 30.237303821913777 36.0 32.0 37.0 2.0 37.0 8 31.915747857295347 36.0 32.0 37.0 17.0 37.0 9 34.57699097534632 38.0 33.0 39.0 27.0 39.0 10-11 35.124089407833054 38.5 35.0 39.0 28.5 39.0 12-13 35.29390127808686 39.0 35.0 39.0 29.0 39.0 14-15 36.39410428985209 40.0 36.0 41.0 29.0 41.0 16-17 36.381605292383306 40.0 36.0 41.0 29.0 41.0 18-19 36.3566916973367 40.0 36.0 41.0 28.5 41.0 20-21 36.42455017828589 40.0 36.0 41.0 29.0 41.0 22-23 36.35901905500277 40.0 36.0 41.0 28.5 41.0 24-25 36.32774072663463 40.0 36.0 41.0 29.0 41.0 26-27 36.34161608162109 40.0 36.0 41.0 29.0 41.0 28-29 36.314703558782426 40.0 36.0 41.0 29.0 41.0 30-31 36.224847250403954 40.0 36.0 41.0 29.0 41.0 32-33 36.1852586001035 40.0 36.0 41.0 29.0 41.0 34-35 36.12116137091253 40.0 36.0 41.0 29.0 41.0 36-37 35.95535374919555 40.0 36.0 41.0 28.0 41.0 38-39 35.83035525057081 40.0 35.0 41.0 27.5 41.0 40-41 35.74108412337959 39.0 35.0 41.0 27.0 41.0 42-43 35.59872062163781 39.0 35.0 41.0 27.0 41.0 44-45 35.455590353021435 39.0 35.0 41.0 27.0 41.0 46-47 35.29005575558825 39.0 35.0 41.0 26.0 41.0 48-49 35.20204429838377 39.0 35.0 40.5 26.0 41.0 50-51 35.06292796459621 39.0 34.5 40.0 26.0 41.0 52-53 34.93263726347528 39.0 34.0 40.0 26.0 41.0 54-55 34.79674736897708 39.0 34.0 40.0 25.0 41.0 56-57 34.58444147742558 38.0 34.0 40.0 24.0 41.0 58-59 34.390925119667685 38.0 34.0 40.0 24.0 41.0 60-61 34.15990692635744 38.0 33.5 40.0 23.5 41.0 62-63 33.912857726064175 37.5 33.0 40.0 23.0 41.0 64-65 33.553331690538215 37.0 33.0 40.0 21.5 41.0 66-67 30.2235472854425 36.0 28.0 39.0 6.5 41.0 68-69 30.53814739462638 35.0 28.5 39.0 7.0 41.0 70-71 30.683872362567982 35.0 28.0 39.0 11.0 40.5 72-73 31.830922608980792 35.0 30.5 38.5 17.0 40.0 74-75 31.893125909784978 35.0 31.5 38.0 16.5 40.0 76-77 31.59303173319664 35.0 31.5 37.0 13.0 39.0 78-79 28.352167165769004 34.5 26.0 36.0 4.5 39.0 80-81 30.04016782598667 34.0 28.5 36.0 7.0 38.5 82-83 30.416904400559943 34.5 30.5 36.0 4.5 37.0 84-85 28.68467916611616 34.5 28.5 35.5 2.0 37.0 86-87 28.326159730054666 34.0 25.5 35.0 2.0 36.5 88-89 29.471972455801772 34.0 29.5 35.0 2.0 36.0 90-91 29.601738353622242 34.0 30.5 35.0 2.0 36.0 92-93 29.45298183458629 34.0 30.0 35.0 2.0 36.0 94-95 29.235980932727927 34.0 30.0 35.0 2.0 35.5 96-97 29.03363119326981 34.0 30.0 35.0 2.0 35.0 98-99 28.81919754624268 34.0 30.0 35.0 2.0 35.0 100 28.59326459165628 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45461818433709666 1101 2 0.4528867657498026 1101 3 0.4780663106672911 1101 4 0.49604914909294706 1101 5 0.49151771174421555 1101 6 0.5201476324850418 1101 7 -11.824987424725396 1101 8 -6.32080685743896 1101 9 -2.110604027620056 1101 10-11 -0.038888671162176536 1101 12-13 0.4934257922362093 1101 14-15 0.7149343824202319 1101 16-17 0.769532473868594 1101 18-19 0.7261631374039723 1101 20-21 0.7551714617383496 1101 22-23 0.7493648213013486 1101 24-25 0.7863179187185523 1101 26-27 0.7588989547587417 1101 28-29 0.7071594960555672 1101 30-31 0.7973623801033938 1101 32-33 0.8356009339504453 1101 34-35 0.828636986392759 1101 36-37 0.865376368485812 1101 38-39 0.8823814600709952 1101 40-41 0.8597903355674745 1101 42-43 0.8448179334055084 1101 44-45 0.7813392693088801 1101 46-47 0.7915336346464485 1101 48-49 0.7305692079638959 1101 50-51 0.8380318849586104 1101 52-53 0.8057802096295177 1101 54-55 0.6677065179381074 1101 56-57 0.6747387933768607 1101 58-59 0.6747887642627362 1101 60-61 0.7008075544139132 1101 62-63 0.6954173869355245 1101 64-65 0.6201329183713966 1101 66-67 -9.72527681793056 1101 68-69 -7.702517095629311 1101 70-71 -6.259651481227451 1101 72-73 -1.2510563375057053 1101 74-75 0.017249290777058945 1101 76-77 0.2982350997663019 1101 78-79 3.3149627439669054 1101 80-81 1.2756268300319356 1101 82-83 0.6367340043628396 1101 84-85 -4.988272765982472 1101 86-87 -5.46808608928556 1101 88-89 -1.0481860697060945 1101 90-91 0.21614644144247208 1101 92-93 0.5921273763416792 1101 94-95 0.6577400449099606 1101 96-97 0.719866954258233 1101 98-99 0.7784174199217553 1101 100 0.796814171602005 1102 1 0.34845830924907517 1102 2 0.40105781896325965 1102 3 0.377185745356023 1102 4 0.4237974532028801 1102 5 0.414911153865269 1102 6 0.43218309419138734 1102 7 -11.871556548859349 1102 8 -6.36958585061069 1102 9 -2.160998556840781 1102 10-11 -0.13222837547301225 1102 12-13 0.4561126633087156 1102 14-15 0.6506238840115159 1102 16-17 0.6170406246862967 1102 18-19 0.643862111389879 1102 20-21 0.6727513018467377 1102 22-23 0.5845343665710061 1102 24-25 0.6904893781972348 1102 26-27 0.6536081762505574 1102 28-29 0.6754621335236166 1102 30-31 0.6806728731665146 1102 32-33 0.7265151959497302 1102 34-35 0.7446585855129726 1102 36-37 0.8348618889693995 1102 38-39 0.8238086319255657 1102 40-41 0.7397783468554309 1102 42-43 0.7041760051813029 1102 44-45 0.6859470022468344 1102 46-47 0.7128689011413698 1102 48-49 0.6095489671108254 1102 50-51 0.6876473665797249 1102 52-53 0.7137998415707187 1102 54-55 0.6433691895013496 1102 56-57 0.7011485604595649 1102 58-59 0.6395809979877001 1102 60-61 0.5899061121237423 1102 62-63 0.5473104808639491 1102 64-65 0.4469634080910865 1102 66-67 -9.788909470575245 1102 68-69 -6.341578841627033 1102 70-71 -5.8233668691062626 1102 72-73 -0.9478140153597288 1102 74-75 -0.06071014004340469 1102 76-77 0.13487159783775482 1102 78-79 3.1883929492037417 1102 80-81 1.199510487527018 1102 82-83 0.6227555889041589 1102 84-85 -5.112126609635478 1102 86-87 -5.558933822923301 1102 88-89 -1.1557655647806158 1102 90-91 0.12835398271653986 1102 92-93 0.4837366629039721 1102 94-95 0.4778437834734284 1102 96-97 0.6167263685073543 1102 98-99 0.6439666142190745 1102 100 0.7202462477971459 1103 1 0.22072428417001788 1103 2 0.2546777201907915 1103 3 0.2657294320416277 1103 4 0.25755065803640065 1103 5 0.2726090358852886 1103 6 0.31484331598142745 1103 7 -11.829772834658016 1103 8 -6.38864092489046 1103 9 -2.2335533518222803 1103 10-11 -0.2591866313978741 1103 12-13 0.3324599294259514 1103 14-15 0.5057451372017212 1103 16-17 0.4549275334936027 1103 18-19 0.4897027625092676 1103 20-21 0.5034079299965271 1103 22-23 0.4972061127774339 1103 24-25 0.5813512131716152 1103 26-27 0.5141877628423401 1103 28-29 0.5255252183567549 1103 30-31 0.5296142146045213 1103 32-33 0.5534723328099318 1103 34-35 0.5397953839539866 1103 36-37 0.536406580317724 1103 38-39 0.6395422794080332 1103 40-41 0.5899618773586042 1103 42-43 0.5440238467005329 1103 44-45 0.523856963006267 1103 46-47 0.5204859882527231 1103 48-49 0.4430380570882235 1103 50-51 0.47159623616455804 1103 52-53 0.4321451988054008 1103 54-55 0.46213174729791007 1103 56-57 0.42190377079731434 1103 58-59 0.4131432513986866 1103 60-61 0.39809133705457356 1103 62-63 0.28945053722582514 1103 64-65 0.2419768754688647 1103 66-67 -9.890044447787265 1103 68-69 -7.978522492003474 1103 70-71 -6.521421696879873 1103 72-73 -1.57547344132713 1103 74-75 -0.3851987957301404 1103 76-77 -0.02321554711948437 1103 78-79 2.977017532176692 1103 80-81 0.9595816717368386 1103 82-83 0.3435890635827654 1103 84-85 -5.175238405598563 1103 86-87 -5.692212762695707 1103 88-89 -1.3362593202828457 1103 90-91 -0.12613322481474043 1103 92-93 0.2556251426129137 1103 94-95 0.37640046690578544 1103 96-97 0.47984025833009625 1103 98-99 0.50908143844131 1103 100 0.5384493746431644 1104 1 0.24171834185643348 1104 2 0.2580784791750368 1104 3 0.2868913730866751 1104 4 0.3061106608347046 1104 5 0.28886576110780027 1104 6 0.3341830440036375 1104 7 -11.807821811834838 1104 8 -6.318177872369851 1104 9 -2.2284028718344615 1104 10-11 -0.2853081647780371 1104 12-13 0.3297174434153263 1104 14-15 0.5286452933542449 1104 16-17 0.545321894802214 1104 18-19 0.5744552982021958 1104 20-21 0.6499442447453561 1104 22-23 0.5906897865706995 1104 24-25 0.7147577483481484 1104 26-27 0.6805439902595793 1104 28-29 0.6414341723395793 1104 30-31 0.6348072352906513 1104 32-33 0.616015864668654 1104 34-35 0.5903504731460458 1104 36-37 0.6544217656261964 1104 38-39 0.7308156701843629 1104 40-41 0.7558957795283376 1104 42-43 0.7181787397562474 1104 44-45 0.6269212167064353 1104 46-47 0.6916723976037105 1104 48-49 0.6452848410515486 1104 50-51 0.6086232183344649 1104 52-53 0.6102010930165349 1104 54-55 0.6020606859237958 1104 56-57 0.6488760056716103 1104 58-59 0.5882901971381287 1104 60-61 0.558619106397984 1104 62-63 0.4662135608673452 1104 64-65 0.3714849626067007 1104 66-67 -9.753634258879497 1104 68-69 -7.870677840271277 1104 70-71 -6.3437883641076525 1104 72-73 -1.471695938289539 1104 74-75 -0.29252823525428084 1104 76-77 0.011967282469420581 1104 78-79 3.0306318063261664 1104 80-81 1.075345885370698 1104 82-83 0.393295801947648 1104 84-85 -5.107333186139435 1104 86-87 -5.5877993498875895 1104 88-89 -1.2660673142825338 1104 90-91 -0.020239836596022798 1104 92-93 0.36847274467582025 1104 94-95 0.4018439022296967 1104 96-97 0.4635070145870408 1104 98-99 0.5249614758465349 1104 100 0.6293875385683307 1105 1 0.04395116591586756 1105 2 0.08927235891853513 1105 3 0.10097019779268379 1105 4 0.11604269617386365 1105 5 0.14820481342066216 1105 6 0.22440817537439983 1105 7 -11.9341496123048 1105 8 -6.487177704032987 1105 9 -2.4342521912459816 1105 10-11 -0.43523727188451744 1105 12-13 0.08485324167014596 1105 14-15 0.2696822991821648 1105 16-17 0.3954422423885049 1105 18-19 0.32554251504198817 1105 20-21 0.350910620102745 1105 22-23 0.35912409923012234 1105 24-25 0.39934695524578956 1105 26-27 0.3418192867163867 1105 28-29 0.3588874290677708 1105 30-31 0.33464963542733983 1105 32-33 0.3720967146958287 1105 34-35 0.4038672144273079 1105 36-37 0.48866421731898413 1105 38-39 0.37313221476915004 1105 40-41 0.4188915482704374 1105 42-43 0.3619909941229267 1105 44-45 0.274892401751444 1105 46-47 0.2587969397130223 1105 48-49 0.2621479432668252 1105 50-51 0.32266040969522436 1105 52-53 0.3558855636449749 1105 54-55 0.28833674690719846 1105 56-57 0.22128231836970258 1105 58-59 0.24772493513395233 1105 60-61 0.2226425044623923 1105 62-63 0.1568157974675799 1105 64-65 5.823813443583958E-4 1105 66-67 -9.96706802922942 1105 68-69 -8.111170725162658 1105 70-71 -6.589546658827121 1105 72-73 -1.7993630743792757 1105 74-75 -0.6203350217354213 1105 76-77 -0.32502136538989035 1105 78-79 2.7139669039701886 1105 80-81 0.6966356164656666 1105 82-83 0.08991624945932486 1105 84-85 -5.318283317087349 1105 86-87 -5.781515449623104 1105 88-89 -1.51533888078961 1105 90-91 -0.268509112169955 1105 92-93 0.07675817231584148 1105 94-95 0.14545988097946605 1105 96-97 0.2123431064555099 1105 98-99 0.214618158100766 1105 100 0.2963739192058483 1106 1 0.14639145990672375 1106 2 0.22193616639664526 1106 3 0.17085958278800106 1106 4 0.14706486279808928 1106 5 0.1202425141767236 1106 6 0.17855644390284908 1106 7 -11.949954152483937 1106 8 -6.517971915302663 1106 9 -2.53502273977017 1106 10-11 -0.45533283737886165 1106 12-13 0.20513707408255755 1106 14-15 0.36251257454605224 1106 16-17 0.3740252169917966 1106 18-19 0.35649672393462595 1106 20-21 0.38174808675763927 1106 22-23 0.38638025124436837 1106 24-25 0.4454734924368893 1106 26-27 0.4081686257569288 1106 28-29 0.39516992355905955 1106 30-31 0.3422060411329326 1106 32-33 0.3881595853558508 1106 34-35 0.46566598182577934 1106 36-37 0.5190626724727849 1106 38-39 0.4918024759016788 1106 40-41 0.5295475378238379 1106 42-43 0.5418740900059831 1106 44-45 0.40188123233150463 1106 46-47 0.502371517796071 1106 48-49 0.4490390282390848 1106 50-51 0.4935422473703994 1106 52-53 0.47716430846024593 1106 54-55 0.48521599963073214 1106 56-57 0.4429251722664702 1106 58-59 0.39470335639052223 1106 60-61 0.401560467409503 1106 62-63 0.30403456729843725 1106 64-65 0.16368829179593547 1106 66-67 -9.909163676083832 1106 68-69 -7.965673526671523 1106 70-71 -6.5029738451923755 1106 72-73 -1.626581608749472 1106 74-75 -0.3722897919845032 1106 76-77 -0.07780690533009604 1106 78-79 2.955031937399472 1106 80-81 0.8625593953962785 1106 82-83 0.2189984374593159 1106 84-85 -5.3629937300793955 1106 86-87 -5.760891040179995 1106 88-89 -1.4058918906408735 1106 90-91 -0.17845111631651633 1106 92-93 0.16457526240817089 1106 94-95 0.28859445946756423 1106 96-97 0.3925084362814104 1106 98-99 0.4180878181358665 1106 100 0.4818188902794027 1107 1 0.09347608455145462 1107 2 0.10126868810931455 1107 3 0.11596374181345936 1107 4 0.12196328398428591 1107 5 0.16114611927362432 1107 6 0.12048258551460123 1107 7 -11.88468943485287 1107 8 -6.464400015645662 1107 9 -2.4921402348816386 1107 10-11 -0.49765543974356774 1107 12-13 0.10744360629001903 1107 14-15 0.2426993914005564 1107 16-17 0.28402384348862597 1107 18-19 0.2993540394218428 1107 20-21 0.29096689704519463 1107 22-23 0.3108401811191115 1107 24-25 0.40060875186304656 1107 26-27 0.3393865826684177 1107 28-29 0.29719721082903305 1107 30-31 0.33298821799758827 1107 32-33 0.3369026257199863 1107 34-35 0.32112223592011446 1107 36-37 0.3948484583999772 1107 38-39 0.38503901952776687 1107 40-41 0.3575726019589496 1107 42-43 0.3972094690137098 1107 44-45 0.3400200112956995 1107 46-47 0.37818353133639704 1107 48-49 0.3807549353630364 1107 50-51 0.39074721097595955 1107 52-53 0.341405042887871 1107 54-55 0.28279276220377625 1107 56-57 0.3402656820829364 1107 58-59 0.31709974668122953 1107 60-61 0.2789671062123844 1107 62-63 0.24095736195358342 1107 64-65 0.13455349837370534 1107 66-67 -9.88400074065397 1107 68-69 -7.998751125175275 1107 70-71 -6.526158782883503 1107 72-73 -1.748863012827126 1107 74-75 -0.49006741620527094 1107 76-77 -0.22821969664424557 1107 78-79 2.7977379462800975 1107 80-81 0.7899113749002744 1107 82-83 0.14582925165395366 1107 84-85 -5.3224266077285165 1107 86-87 -5.741700426704039 1107 88-89 -1.5228469958091786 1107 90-91 -0.3609587450458456 1107 92-93 0.05720309053543815 1107 94-95 0.16157336317375837 1107 96-97 0.23646327781199616 1107 98-99 0.30647890809997946 1107 100 0.40498059585376467 1108 1 0.05620374489050661 1108 2 0.07275866346708071 1108 3 0.08719515381354626 1108 4 -0.019076733265748658 1108 5 -0.02096088343274971 1108 6 0.049039784472142856 1108 7 -12.005356215067536 1108 8 -6.536674447748091 1108 9 -2.625559989867863 1108 10-11 -0.5767878112064224 1108 12-13 -0.029976510588696215 1108 14-15 0.19732667388402803 1108 16-17 0.20300597277552157 1108 18-19 0.23608679779426467 1108 20-21 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2216 30-31 -1.954309299353966 2216 32-33 -2.912850148727159 2216 34-35 -2.0186637521198847 2216 36-37 -1.7037262395063664 2216 38-39 -1.926951928589851 2216 40-41 -1.8597032633514061 2216 42-43 -1.5184500805704246 2216 44-45 -1.6445950297762977 2216 46-47 -1.8495081157810986 2216 48-49 -1.9359512709328328 2216 50-51 -2.542990398787353 2216 52-53 -2.2480538478110645 2216 54-55 -1.802852844054705 2216 56-57 -1.8684854713808008 2216 58-59 -2.204818417147152 2216 60-61 -2.357443865103658 2216 62-63 -2.1653299317253882 2216 64-65 -1.8343588617398652 2216 66-67 1.3996023070580854 2216 68-69 0.5796600318590279 2216 70-71 0.3096018815846513 2216 72-73 -1.4231945535594726 2216 74-75 -2.2013014463650507 2216 76-77 -1.8140477724299267 2216 78-79 0.6773032841304172 2216 80-81 -0.7270103018640448 2216 82-83 -1.5057054404761772 2216 84-85 0.07205616366884726 2216 86-87 -0.026223087965373537 2216 88-89 -1.102949722128205 2216 90-91 -1.3934461726382636 2216 92-93 -1.4704690451321731 2216 94-95 -1.487510547815532 2216 96-97 -1.4707491454237278 2216 98-99 -1.5279465934448986 2216 100 -2.678718593966469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 176495.0 3 31518.0 4 18459.0 5 16179.0 6 14666.0 7 15862.0 8 17591.0 9 20309.0 10 22401.0 11 25007.0 12 27383.0 13 30738.0 14 34291.0 15 37179.0 16 39131.0 17 39434.0 18 37674.0 19 35616.0 20 34519.0 21 34362.0 22 36237.0 23 42129.0 24 50139.0 25 63996.0 26 79395.0 27 96597.0 28 118925.0 29 147027.0 30 177636.0 31 218118.0 32 286316.0 33 393758.0 34 574853.0 35 737422.0 36 872531.0 37 1363657.0 38 1385490.0 39 389306.0 40 555.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.49418757406493 20.48376042011399 14.953630541193599 23.06842146462748 2 15.178144298793498 20.155637972844655 40.74687267200433 23.919345056357514 3 18.426136814935692 25.05716604921091 30.285781813931536 26.230915321921866 4 12.876619373706772 16.98711991931529 38.47771542108334 31.6585452858946 5 13.368710042505521 37.669763452536756 35.40159263642327 13.55993386853446 6 30.874646778231295 37.59086835436466 17.865559587619597 13.668925279784446 7 27.327080119605508 32.47190074789996 22.229107418319526 17.971911714175008 8 24.93950264894256 36.35437157210198 20.457681171436906 18.24844460751855 9 24.771916882315814 17.53541728093902 21.703389982643458 35.98927585410171 10-11 23.873816803288587 27.22116684689627 28.714586432139583 20.19042991767556 12-13 24.199708868807697 24.62522121876542 28.857620677314618 22.317449235112267 14-15 21.64952774160486 26.955077948174722 26.008610984435936 25.386783325784485 16-17 21.300807128092437 30.231205810237398 26.802793790686547 21.665193270983618 18-19 21.297192098930363 29.285257553983968 28.527053361524317 20.890496985561356 20-21 22.34094962217324 28.375832803224064 28.189274807289593 21.093942767313102 22-23 22.098861912350422 28.170726966546518 28.196034018774103 21.534377102328957 24-25 21.884194309083895 28.326624607495305 28.129450447577725 21.659730635843076 26-27 21.621657748917183 28.39405020721264 28.18038325863148 21.803908785238693 28-29 21.62023639460197 28.44739975365822 28.33572326444572 21.59664058729409 30-31 21.894300340402133 28.636308277737243 27.967760404013948 21.501630977846673 32-33 21.616705522691397 28.43857414931265 28.310069333836847 21.634650994159102 34-35 21.80176512767316 28.280555492415242 28.22321906428809 21.69446031562351 36-37 22.012473752561785 28.401650880481 27.911504974526014 21.674370392431204 38-39 21.80486920983988 28.49149911422065 28.077726443286938 21.625905232652524 40-41 21.770490156079745 28.289364670941808 28.19496206912629 21.745183103852163 42-43 21.696859167550862 28.336012885095563 28.151876438535716 21.815251508817855 44-45 21.80003707917605 28.41957405958408 28.050073806416346 21.730315054823517 46-47 21.81889144817371 28.42163748494333 27.991336244370036 21.768134822512927 48-49 21.74878000427491 28.33203643875547 28.01898007905353 21.90020347791609 50-51 21.723521413139267 28.32895586774653 27.988455708535636 21.959067010578565 52-53 21.75672948672539 28.319240282930807 28.02455674614719 21.899473484196616 54-55 21.823399520010177 28.4915385084799 27.894363493651742 21.790698477858186 56-57 21.746096516936742 28.321969975576657 27.914681730302483 22.01725177718412 58-59 21.73047675332874 28.102698843529893 28.130685874698692 22.036138528442674 60-61 21.858231204096086 28.31381241214423 27.88256683237752 21.94538955138216 62-63 21.66925371530514 28.390063175992513 27.937283922458672 22.003399186243673 64-65 21.778589654185293 28.196086922464993 27.95915809352173 22.06616532982798 66-67 21.641838127939838 28.19568084235301 27.92053358899082 22.241947440716334 68-69 22.04388559079794 28.309899337479056 27.77848069441727 21.867734377305734 70-71 21.81268632451891 28.1102702123256 27.926800799332792 22.1502426638227 72-73 21.687538394488666 28.21948825946341 28.02845943343183 22.064513912616096 74-75 21.679942200958866 28.25802508663334 27.902980476162735 22.159052236245056 76-77 21.74176524606425 28.19279714923752 27.814217390012775 22.251220214685457 78-79 21.84376007326595 28.169803522793295 27.962618142062507 22.023818261878255 80-81 21.64065943326866 28.197562627077684 27.972094492033158 22.189683447620506 82-83 21.6155964717549 28.132374027871453 28.100627364693732 22.151402135679916 84-85 21.706812106449192 28.036412064753897 27.972209535330595 22.28456629346632 86-87 21.786259389167704 28.12374860875807 27.917810899587447 22.172181102486782 88-89 21.683939006618363 27.997903077249635 27.975249698928984 22.34290821720302 90-91 21.589212174533433 27.966360113728445 28.012597489181356 22.431830222556762 92-93 21.69734899848514 28.028163847875287 27.9651812997175 22.309305853922076 94-95 21.671980352814984 27.83873861249182 28.047751493848633 22.441529540844563 96-97 21.642303758318263 27.896173139566628 28.002615632303474 22.458907469811642 98-99 21.63710113690162 27.865930219740022 28.003921993833895 22.493046649524466 100 21.70001743634121 27.78948524046006 27.972579738971515 22.537917584227216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1259.0 1 1073.5 2 977.0 3 1063.0 4 1010.5 5 925.0 6 845.5 7 780.5 8 835.0 9 937.5 10 905.5 11 856.5 12 899.5 13 966.5 14 1069.5 15 1260.5 16 1535.0 17 1951.5 18 2696.0 19 3859.5 20 5556.5 21 8184.0 22 12668.5 23 19315.5 24 27874.5 25 38456.5 26 52814.5 27 70373.5 28 92160.0 29 116392.5 30 138203.5 31 159713.0 32 180800.5 33 200660.0 34 219187.5 35 236508.0 36 251251.5 37 263243.5 38 273848.0 39 279775.0 40 283591.0 41 284246.0 42 281609.0 43 279949.5 44 274929.0 45 268896.5 46 262909.5 47 254308.0 48 246586.5 49 241478.5 50 235162.0 51 224877.5 52 213078.5 53 202959.5 54 194339.0 55 183053.5 56 167444.0 57 150124.5 58 135043.0 59 122784.0 60 107550.0 61 90498.5 62 76634.0 63 63359.0 64 50995.5 65 40901.5 66 32176.0 67 24884.0 68 19273.0 69 14851.5 70 11140.0 71 8441.5 72 6390.5 73 4700.0 74 3385.5 75 2387.5 76 1637.5 77 1103.5 78 796.5 79 627.5 80 452.5 81 263.5 82 170.0 83 130.0 84 96.5 85 71.5 86 68.5 87 56.0 88 46.5 89 37.0 90 35.5 91 37.5 92 29.0 93 22.0 94 16.5 95 15.5 96 19.0 97 25.0 98 26.5 99 27.5 100 52.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.017861522444882094 2 0.0024409455835739087 3 0.0013689959357610281 4 0.002195559519616743 5 0.0018339379516798678 6 0.003151273663449914 7 11.671994773018536 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0664089596392877E-4 18-19 0.0 20-21 6.457527998872774E-6 22-23 0.0 24-25 0.0 26-27 1.226930319785827E-4 28-29 0.0 30-31 0.0 32-33 6.457527998872774E-6 34-35 6.457527998872774E-5 36-37 3.228763999436387E-5 38-39 2.5830111995491096E-5 40-41 0.0 42-43 1.937258399661832E-5 44-45 1.3560808797632827E-4 46-47 8.975963918433157E-4 48-49 5.940925758962951E-4 50-51 3.4224898394025704E-4 52-53 2.3892853595829265E-4 54-55 3.680790959357481E-4 56-57 0.0013496233517644098 58-59 3.745366239346209E-4 60-61 0.001252760431781318 62-63 0.002137441767626888 64-65 8.975963918433157E-4 66-67 5.836262145157222 68-69 5.478424688627686 70-71 0.0024990633355637636 72-73 0.001168812567795972 74-75 0.0027379918715220563 76-77 0.005308088015073421 78-79 5.424200826021151 80-81 0.004436321735225596 82-83 0.004584844879199669 84-85 5.836455870997188 86-87 0.003467692535394679 88-89 0.0016337545837148118 90-91 0.00319001883144315 92-93 0.0036162156793687534 94-95 0.004081157695287593 96-97 0.0028735999594983845 98-99 0.005301630487074547 100 0.005824690254983242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7742901.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.46468923180562 #Duplication Level Percentage of deduplicated Percentage of total 1 91.02053175366277 78.70061991794147 2 6.33030855166916 10.946963233230308 3 1.3946683333234295 3.617686920667519 4 0.533016042053537 1.8434826572690446 5 0.2573460564921825 1.1125673399813627 6 0.1414898034603935 0.7340323131403307 7 0.08818963467392057 0.5337702548882909 8 0.061092533494810974 0.4225877538410005 9 0.04117071689492381 0.3203831917593213 >10 0.13088111191814292 1.6835628376371419 >50 0.001173618041887821 0.06885586125082373 >100 1.3184431479476124E-4 0.015487718393466025 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.5830111995491096E-5 0.0 4 0.0 0.0 0.0 3.874516799323664E-5 0.0 5 0.0 0.0 0.0 3.874516799323664E-5 0.0 6 0.0 0.0 0.0 3.874516799323664E-5 0.0 7 0.0 0.0 0.0 6.457527998872774E-5 0.0 8 0.0 0.0 0.0 6.457527998872774E-5 0.0 9 9.040539198421883E-5 0.0 0.0 1.291505599774555E-4 0.0 10-11 9.040539198421883E-5 0.0 0.0 1.6143819997181934E-4 0.0 12-13 9.040539198421883E-5 0.0 0.0 1.6789572797069213E-4 0.0 14-15 9.040539198421883E-5 0.0 0.0 1.8726831196731045E-4 0.0 16-17 9.040539198421883E-5 0.0 0.0 2.1955595196167433E-4 0.0 18-19 1.0977797598083717E-4 1.2915055997745548E-5 0.0 2.3892853595829263E-4 0.0 20-21 1.1623550397970994E-4 1.2915055997745548E-5 0.0 2.453860639571654E-4 0.0 22-23 1.1623550397970994E-4 1.2915055997745548E-5 0.0 3.3579145594138425E-4 0.0 24-25 1.1623550397970994E-4 1.2915055997745548E-5 0.0 5.618049359019314E-4 0.0 26-27 1.1623550397970994E-4 1.2915055997745548E-5 0.0 0.00100091683982528 0.0 28-29 1.226930319785827E-4 1.2915055997745548E-5 0.0 0.0027831945675141656 0.0 30-31 1.291505599774555E-4 1.2915055997745548E-5 0.0 0.008272093366556023 0.0 32-33 1.291505599774555E-4 1.2915055997745548E-5 0.0 0.02011519971648869 0.0 34-35 1.6789572797069213E-4 1.2915055997745548E-5 0.0 0.039616934273084464 0.0 36-37 1.6789572797069213E-4 1.2915055997745548E-5 0.0 0.07091011495562193 0.0 38-39 1.6789572797069213E-4 1.937258399661832E-5 0.0 0.11330378626822168 0.0 40-41 1.6789572797069213E-4 2.5830111995491096E-5 0.0 0.15798342249242242 0.0 42-43 1.6789572797069213E-4 2.5830111995491096E-5 0.0 0.20434847352432894 0.0 44-45 1.743532559695649E-4 3.228763999436387E-5 6.457527998872774E-6 0.2632798740420419 0.0 46-47 1.8081078396843767E-4 3.874516799323664E-5 1.2915055997745548E-5 0.33353132114177875 0.0 48-49 1.8726831196731045E-4 3.874516799323664E-5 1.2915055997745548E-5 0.4083224104247233 0.0 50-51 1.9372583996618323E-4 3.874516799323664E-5 1.2915055997745548E-5 0.4841531617154862 0.0 52-53 1.9372583996618323E-4 3.874516799323664E-5 1.2915055997745548E-5 0.5604876001901613 0.0 54-55 1.9372583996618323E-4 3.874516799323664E-5 1.2915055997745548E-5 0.6455461589913134 0.0 56-57 1.9372583996618323E-4 3.874516799323664E-5 1.2915055997745548E-5 0.7347052480717498 0.0 58-59 1.9372583996618323E-4 3.874516799323664E-5 1.2915055997745548E-5 0.8356234956381335 0.0 60-61 2.0018336796505599E-4 3.874516799323664E-5 1.2915055997745548E-5 0.9370906330844214 0.0 62-63 2.0664089596392877E-4 3.874516799323664E-5 1.2915055997745548E-5 1.0384609076107263 0.0 64-65 2.1309842396280155E-4 3.874516799323664E-5 1.2915055997745548E-5 1.1495691343593313 0.0 66-67 2.195559519616743E-4 3.874516799323664E-5 1.2915055997745548E-5 1.2730822723937707 0.0 68-69 2.3247100795941987E-4 4.5202695992109416E-5 1.2915055997745548E-5 1.3987845124198284 0.0 70-71 2.3247100795941987E-4 5.166022399098219E-5 1.2915055997745548E-5 1.5484312662657058 6.457527998872774E-6 72-73 2.3247100795941987E-4 5.166022399098219E-5 1.2915055997745548E-5 1.7059432892142106 1.2915055997745548E-5 74-75 2.8413123195040205E-4 5.811775198985497E-5 1.2915055997745548E-5 1.850934165372901 1.2915055997745548E-5 76-77 2.8413123195040205E-4 6.457527998872774E-5 1.2915055997745548E-5 2.0025375502024367 1.2915055997745548E-5 78-79 2.8413123195040205E-4 7.749033598647328E-5 1.2915055997745548E-5 2.182108488795091 1.2915055997745548E-5 80-81 2.8413123195040205E-4 8.394786398534606E-5 1.2915055997745548E-5 2.39711188351756 1.2915055997745548E-5 82-83 2.8413123195040205E-4 9.040539198421883E-5 1.2915055997745548E-5 2.618514688486912 1.2915055997745548E-5 84-85 2.8413123195040205E-4 9.040539198421883E-5 1.2915055997745548E-5 2.8408667500720983 1.2915055997745548E-5 86-87 2.8413123195040205E-4 9.040539198421883E-5 1.2915055997745548E-5 3.104417840290093 1.2915055997745548E-5 88 2.8413123195040205E-4 9.040539198421883E-5 1.2915055997745548E-5 3.3250199117875847 1.2915055997745548E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7320 0.0 43.594654 1 GTATCAA 11395 0.0 38.478283 1 TCAACGC 12180 0.0 34.26462 4 ATCAACG 12350 0.0 33.87589 3 CAACGCA 13625 0.0 30.630684 5 TATCAAC 14165 0.0 29.96909 2 AACGCAG 14880 0.0 28.287113 6 ACGCAGA 17195 0.0 23.912596 7 GTACATG 22810 0.0 23.668547 1 CGCAGAG 17420 0.0 22.8742 8 TACATGG 22945 0.0 22.54078 2 ACATGGG 23235 0.0 21.333801 3 GCAGAGT 20115 0.0 18.932598 9 CATGGGG 16250 0.0 17.268824 4 GAGTACA 17240 0.0 17.173027 1 GTGGTAT 4505 0.0 17.16874 1 AGAGTAC 17645 0.0 16.469093 10-11 CAGAGTA 19295 0.0 16.314835 10-11 GTATAGG 2315 0.0 15.709546 1 CCGATCG 265 1.264809E-5 15.360836 9 >>END_MODULE