FastQCFastQC Report
Wed 25 May 2016
SRR2049501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742901
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173990.22470905930477478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173200.22368876988095288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95670.12355834073043165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134200.055.9996151
GTATCAA231850.039.9689371
ATCAACG292950.031.1803573
TCAACGC295000.030.9636784
TATCAAC305500.030.1767372
CAACGCA307450.029.6716445
GTGGTAT53850.028.5060181
AACGCAG322800.028.3323786
ACGCAGA369950.024.5559627
CGCAGAG374750.024.040788
TGGTATC64300.023.0541232
GCAGAGT405100.022.0076269
GTACATG240300.021.8487071
GAGTACT263900.021.12094912-13
TACATGG243850.020.688122
CAGAGTA396000.020.03297210-11
AGAGTAC377450.019.80351810-11
ACATGGG248050.019.7876453
GTACTTT289750.019.11499814-15
AGTACTT281300.018.62830212-13
Produced by FastQC (version 0.11.5)