##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049501_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7742901
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.729356090178605	31.0	31.0	34.0	30.0	34.0
2	31.769773499622428	31.0	31.0	34.0	30.0	34.0
3	31.845260710423652	33.0	31.0	34.0	30.0	34.0
4	35.55952504106665	37.0	35.0	37.0	33.0	37.0
5	35.712511499242986	37.0	35.0	37.0	33.0	37.0
6	35.80800606904312	37.0	35.0	37.0	35.0	37.0
7	35.78366958327376	37.0	35.0	37.0	33.0	37.0
8	35.77724718422721	37.0	35.0	37.0	33.0	37.0
9	37.50401961745346	39.0	37.0	39.0	35.0	39.0
10-11	37.470851364365885	39.0	37.0	39.0	34.5	39.0
12-13	37.47158616647688	39.0	37.0	39.0	34.5	39.0
14-15	38.75848709676128	40.0	38.0	41.0	35.0	41.0
16-17	38.767263587639825	40.0	38.0	41.0	35.0	41.0
18-19	38.82833604614085	40.0	38.0	41.0	35.0	41.0
20-21	38.857462338728084	40.0	38.0	41.0	35.0	41.0
22-23	38.843419346314775	40.0	38.0	41.0	35.0	41.0
24-25	38.818276121572524	40.0	38.0	41.0	35.0	41.0
26-27	38.73613810895942	40.0	38.0	41.0	34.5	41.0
28-29	38.656540552436354	40.0	38.0	41.0	34.0	41.0
30-31	38.55480478182531	40.0	38.0	41.0	34.0	41.0
32-33	38.437203446098565	40.0	38.0	41.0	34.0	41.0
34-35	38.35136662602299	40.0	38.0	41.0	34.0	41.0
36-37	38.22924418121838	40.0	38.0	41.0	34.0	41.0
38-39	38.14760682075104	40.0	38.0	41.0	33.5	41.0
40-41	38.03529355470256	40.0	38.0	41.0	33.0	41.0
42-43	37.9084463949623	40.0	37.0	41.0	33.0	41.0
44-45	37.74492686914117	40.0	37.0	41.0	33.0	41.0
46-47	37.52769465862988	40.0	37.0	41.0	33.0	41.0
48-49	37.32297261711082	40.0	37.0	41.0	32.0	41.0
50-51	37.1212134185882	40.0	37.0	41.0	32.0	41.0
52-53	37.023004491469024	40.0	36.0	41.0	31.0	41.0
54-55	36.89874370084287	40.0	36.0	41.0	31.0	41.0
56-57	36.73016069816727	39.0	36.0	41.0	31.0	41.0
58-59	36.56202242544494	39.0	35.0	41.0	31.0	41.0
60-61	36.376880642022925	39.0	35.0	41.0	31.0	41.0
62-63	36.17867999345465	39.0	35.0	41.0	31.0	41.0
64-65	35.91268614954524	38.0	35.0	40.0	30.0	41.0
66-67	35.66100167107909	38.0	35.0	40.0	30.0	41.0
68-69	35.35402900024164	37.0	35.0	40.0	29.5	41.0
70-71	35.06339749145701	37.0	34.5	39.5	29.0	41.0
72-73	34.747485922911835	36.0	34.0	39.0	29.0	41.0
74-75	34.40370056391009	36.0	34.0	39.0	29.0	40.5
76-77	34.110475711881115	35.0	34.0	38.5	29.0	40.0
78-79	33.78241042988926	35.0	34.0	37.0	29.0	39.0
80-81	33.47196909788721	35.0	34.0	37.0	29.0	39.0
82-83	33.17719850738115	35.0	34.0	36.0	29.0	39.0
84-85	32.905269291186855	35.0	34.0	36.0	28.0	37.0
86-87	32.63879558837185	35.0	33.0	36.0	27.5	37.0
88-89	32.43080836239544	35.0	33.0	35.0	27.0	37.0
90-91	32.28356561707298	35.0	33.0	35.0	27.0	36.0
92-93	32.13198147567688	35.0	33.0	35.0	27.0	36.0
94-95	31.95892075851157	35.0	33.0	35.0	27.0	36.0
96-97	31.797119387165097	35.0	33.0	35.0	26.0	36.0
98-99	31.62420131679328	35.0	33.0	35.0	26.0	35.0
100	31.517562732624373	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3541526746102903
1101	2	0.427209665979845
1101	3	0.474288586015124
1101	4	0.3654395740493541
1101	5	0.2937295515401388
1101	6	0.2996595265585711
1101	7	0.30712042257376737
1101	8	0.3393400429645297
1101	9	0.35339332901538256
1101	10-11	0.3710097005078552
1101	12-13	0.37567066262305815
1101	14-15	0.5086425390149003
1101	16-17	0.5393580521995247
1101	18-19	0.4731315491628152
1101	20-21	0.4637643617587912
1101	22-23	0.5033242728186167
1101	24-25	0.503413998250771
1101	26-27	0.482938433974212
1101	28-29	0.5417155588610001
1101	30-31	0.5188814420690946
1101	32-33	0.5516553452388138
1101	34-35	0.5688925318711213
1101	36-37	0.5934492409248051
1101	38-39	0.582041472818517
1101	40-41	0.6104145347939749
1101	42-43	0.549961007022219
1101	44-45	0.6293462257518172
1101	46-47	0.7267596336934972
1101	48-49	0.7047750210541963
1101	50-51	0.6883792964966489
1101	52-53	0.7052259423359786
1101	54-55	0.6392086316953609
1101	56-57	0.6048884130067194
1101	58-59	0.6164386288096111
1101	60-61	0.6767343737114757
1101	62-63	0.6457996504319325
1101	64-65	0.65230425705154
1101	66-67	0.6767260804921094
1101	68-69	0.6435229625822458
1101	70-71	0.6522901920639015
1101	72-73	0.5905778732035003
1101	74-75	0.6058941451863262
1101	76-77	0.6069017671750814
1101	78-79	0.6015504597856136
1101	80-81	0.5599945129181734
1101	82-83	0.5474312773857264
1101	84-85	0.5994602411901084
1101	86-87	0.6630907622441882
1101	88-89	0.6332301054601501
1101	90-91	0.6072051045454572
1101	92-93	0.6083597011981965
1101	94-95	0.5805423413723823
1101	96-97	0.627962919436591
1101	98-99	0.6760238437848223
1101	100	0.7320790583859704
1102	1	0.22922203370744398
1102	2	0.3136459958108375
1102	3	0.4114869304357036
1102	4	0.379250842466746
1102	5	0.2955212978440258
1102	6	0.26954569939915984
1102	7	0.28941678334118137
1102	8	0.2988389468745396
1102	9	0.35389673829018164
1102	10-11	0.32836290682013214
1102	12-13	0.30425108151360547
1102	14-15	0.4169580496480165
1102	16-17	0.3719659153756183
1102	18-19	0.39385928876818355
1102	20-21	0.4217428083191166
1102	22-23	0.4142514954563339
1102	24-25	0.41747825910803726
1102	26-27	0.36327437061238754
1102	28-29	0.3894032244833383
1102	30-31	0.4572464383344652
1102	32-33	0.4764155588487853
1102	34-35	0.4740936987659481
1102	36-37	0.46508223277179894
1102	38-39	0.44789803883734436
1102	40-41	0.45947417171143456
1102	42-43	0.4784816767378217
1102	44-45	0.5544048693604964
1102	46-47	0.5051063771812281
1102	48-49	0.5005584155945897
1102	50-51	0.5356266551651885
1102	52-53	0.45042902392579975
1102	54-55	0.44178652066151614
1102	56-57	0.4457240218627163
1102	58-59	0.43248945861606813
1102	60-61	0.41338506190825086
1102	62-63	0.42651130951514915
1102	64-65	0.38947669963377507
1102	66-67	0.4602697386966952
1102	68-69	0.41600906281993133
1102	70-71	0.42939143617828535
1102	72-73	0.47724914194163404
1102	74-75	0.48574917264567574
1102	76-77	0.439612994722971
1102	78-79	0.3742870254545494
1102	80-81	0.3381865986187549
1102	82-83	0.3718773400876074
1102	84-85	0.37433171607382576
1102	86-87	0.3793412743279063
1102	88-89	0.338699451144727
1102	90-91	0.3324274448567337
1102	92-93	0.3640827682674299
1102	94-95	0.34494208692205675
1102	96-97	0.3619346869187581
1102	98-99	0.45299368345098756
1102	100	0.5257093443898881
1103	1	0.27681478525661163
1103	2	0.3364971245727588
1103	3	0.3944500483260889
1103	4	0.35137448951952877
1103	5	0.2615645324197047
1103	6	0.2612583354483746
1103	7	0.26920164062612173
1103	8	0.29917474425568
1103	9	0.29949543359336417
1103	10-11	0.3001998432687145
1103	12-13	0.28694103455110564
1103	14-15	0.42746088478077837
1103	16-17	0.3997946827301817
1103	18-19	0.4014657701099438
1103	20-21	0.4093827664606451
1103	22-23	0.4241335343437953
1103	24-25	0.4132734613854083
1103	26-27	0.39723795180333354
1103	28-29	0.42708385107220437
1103	30-31	0.4205329994440916
1103	32-33	0.3696084561960902
1103	34-35	0.4045953968978253
1103	36-37	0.427429777758384
1103	38-39	0.3716850253865047
1103	40-41	0.39933647918844883
1103	42-43	0.3608141607517652
1103	44-45	0.394498468396435
1103	46-47	0.3916154093587423
1103	48-49	0.38444908211963735
1103	50-51	0.390217927911344
1103	52-53	0.38298927074077227
1103	54-55	0.34741232774303654
1103	56-57	0.4085377567086468
1103	58-59	0.4329220141487724
1103	60-61	0.38280142250878413
1103	62-63	0.41283079475476114
1103	64-65	0.39851686656859187
1103	66-67	0.4588712821948988
1103	68-69	0.45201281975784013
1103	70-71	0.43466764278147707
1103	72-73	0.4603110767506209
1103	74-75	0.4515314935920145
1103	76-77	0.4252306743537133
1103	78-79	0.46281846871497123
1103	80-81	0.39554072503126037
1103	82-83	0.39628679173111436
1103	84-85	0.4002051616364781
1103	86-87	0.4219657882073946
1103	88-89	0.4244327804541044
1103	90-91	0.40610164372536417
1103	92-93	0.4212146921095794
1103	94-95	0.42628252986422766
1103	96-97	0.4793802420836144
1103	98-99	0.42175328956830427
1103	100	0.40715740469991246
1104	1	0.26606833078031045
1104	2	0.3040468159661813
1104	3	0.3616341379024668
1104	4	0.31219693952108685
1104	5	0.2020975999489707
1104	6	0.24347767321938107
1104	7	0.20034603189917277
1104	8	0.22820256051870302
1104	9	0.27800044765594123
1104	10-11	0.29473952513238544
1104	12-13	0.2941525358283599
1104	14-15	0.4041383246502903
1104	16-17	0.41856633665607745
1104	18-19	0.3770903098881604
1104	20-21	0.36467334732319046
1104	22-23	0.3845188802635562
1104	24-25	0.37209699344357006
1104	26-27	0.4121535559241778
1104	28-29	0.3896161057280878
1104	30-31	0.37860238355923315
1104	32-33	0.3369968963358758
1104	34-35	0.34585209868865263
1104	36-37	0.3948649875475425
1104	38-39	0.3956695550737166
1104	40-41	0.43025567131932974
1104	42-43	0.42285711302299234
1104	44-45	0.43427390134748123
1104	46-47	0.4540312253957737
1104	48-49	0.4535210496814912
1104	50-51	0.5010726630299871
1104	52-53	0.43987569952319205
1104	54-55	0.38270020627586376
1104	56-57	0.3861670367360972
1104	58-59	0.38858873546438844
1104	60-61	0.39547347061564864
1104	62-63	0.3818383532924585
1104	64-65	0.3966989687254312
1104	66-67	0.3814112314422431
1104	68-69	0.3766630043376864
1104	70-71	0.3503681672126504
1104	72-73	0.3972277950201075
1104	74-75	0.3848122099763245
1104	76-77	0.3117148680305277
1104	78-79	0.3355059232504658
1104	80-81	0.30964413810594493
1104	82-83	0.3212493975687636
1104	84-85	0.31361699874793203
1104	86-87	0.3491020411490595
1104	88-89	0.31215630048151155
1104	90-91	0.3258866862928613
1104	92-93	0.3342214609431551
1104	94-95	0.3299573346248401
1104	96-97	0.3424657920015264
1104	98-99	0.393279173466869
1104	100	0.4375250673807791
1105	1	0.23376236186355825
1105	2	0.2641823491586024
1105	3	0.3753640955096209
1105	4	0.3017711324469303
1105	5	0.20672479910005137
1105	6	0.24686953912353715
1105	7	0.20490822188399704
1105	8	0.2367643906463357
1105	9	0.25926613649556174
1105	10-11	0.269220838580182
1105	12-13	0.2106863778250343
1105	14-15	0.3508599539758208
1105	16-17	0.32573674356377325
1105	18-19	0.3293262097705991
1105	20-21	0.30185534791787205
1105	22-23	0.3184912792516883
1105	24-25	0.31638837187370683
1105	26-27	0.36589160503930884
1105	28-29	0.3754379862095618
1105	30-31	0.37061411289047186
1105	32-33	0.3426260537814301
1105	34-35	0.3266538216644008
1105	36-37	0.3650686110803676
1105	38-39	0.36047013952853746
1105	40-41	0.4054778831711019
1105	42-43	0.3946165093115752
1105	44-45	0.43019178489258536
1105	46-47	0.4159711787501408
1105	48-49	0.42959505516181906
1105	50-51	0.45353395092115534
1105	52-53	0.43762863129739316
1105	54-55	0.35580561771335084
1105	56-57	0.4063505461147159
1105	58-59	0.36561522285259684
1105	60-61	0.35910608115431586
1105	62-63	0.3673512702992383
1105	64-65	0.3367130079574565
1105	66-67	0.4028481197156566
1105	68-69	0.37951154112995056
1105	70-71	0.3724607079994442
1105	72-73	0.3250170196257045
1105	74-75	0.39139896545712816
1105	76-77	0.31878437261317316
1105	78-79	0.3147002855514387
1105	80-81	0.28273201370046763
1105	82-83	0.31153228441338143
1105	84-85	0.3223244486848529
1105	86-87	0.40327160867199296
1105	88-89	0.3745607649379181
1105	90-91	0.340349584285363
1105	92-93	0.31677454821291207
1105	94-95	0.40116745335435056
1105	96-97	0.433787356834209
1105	98-99	0.4657436979323002
1105	100	0.5063685957482917
1106	1	0.1886124152924573
1106	2	0.20664604345441262
1106	3	0.2909160655848133
1106	4	0.241119801300556
1106	5	0.2094308798261295
1106	6	0.22737123782155066
1106	7	0.19460715424158082
1106	8	0.19754822097394253
1106	9	0.21022160047763094
1106	10-11	0.20799428263982378
1106	12-13	0.19164830050711146
1106	14-15	0.29924689951807437
1106	16-17	0.27968312209292634
1106	18-19	0.29446185771261923
1106	20-21	0.25917483009082076
1106	22-23	0.23011920977776157
1106	24-25	0.24284048178520834
1106	26-27	0.2624863918491229
1106	28-29	0.26812941686749525
1106	30-31	0.26962954393818706
1106	32-33	0.29481156496880345
1106	34-35	0.2718874864144638
1106	36-37	0.3020382337466003
1106	38-39	0.3312266189277082
1106	40-41	0.37672710255067443
1106	42-43	0.358674994797056
1106	44-45	0.4000745020063263
1106	46-47	0.36408291913065227
1106	48-49	0.3636864725540718
1106	50-51	0.3837409366115594
1106	52-53	0.3744782215835656
1106	54-55	0.30812615087477724
1106	56-57	0.33235213428287125
1106	58-59	0.3463195543722364
1106	60-61	0.3497955180350729
1106	62-63	0.3473321734583621
1106	64-65	0.3668456133062179
1106	66-67	0.4000479878717016
1106	68-69	0.39856019315444513
1106	70-71	0.3757703949387263
1106	72-73	0.36939635109892066
1106	74-75	0.3418695416514481
1106	76-77	0.3471678205834863
1106	78-79	0.35194312317618426
1106	80-81	0.3204544867984751
1106	82-83	0.35280372083801126
1106	84-85	0.36814346794284347
1106	86-87	0.42551248710284995
1106	88-89	0.3885613996305395
1106	90-91	0.3438343425347128
1106	92-93	0.38989331474662237
1106	94-95	0.41238867814293556
1106	96-97	0.4215831959009009
1106	98-99	0.4691887046753429
1106	100	0.4963223512039576
1107	1	0.15346275025192568
1107	2	0.15245322505484893
1107	3	0.22305796292881652
1107	4	0.2180309577714965
1107	5	0.1722664371169529
1107	6	0.160844565224032
1107	7	0.16606858373670264
1107	8	0.1849950487858365
1107	9	0.20204546120611155
1107	10-11	0.16763609616098307
1107	12-13	0.14683342813993505
1107	14-15	0.21063341096810717
1107	16-17	0.23669106032851772
1107	18-19	0.22814269131841058
1107	20-21	0.2426929622899081
1107	22-23	0.23112096503652424
1107	24-25	0.255367698678036
1107	26-27	0.2367905984621217
1107	28-29	0.23523176940439328
1107	30-31	0.22564978532413704
1107	32-33	0.2761619109060831
1107	34-35	0.2583353161858497
1107	36-37	0.2871563694858139
1107	38-39	0.29632847346334756
1107	40-41	0.28175420923987105
1107	42-43	0.2670290257054688
1107	44-45	0.2718059686915524
1107	46-47	0.300199704003127
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2216	92-93	-1.3452364563735522
2216	94-95	-1.4554933192334722
2216	96-97	-1.505465419500755
2216	98-99	-1.517135891887989
2216	100	-1.537585946178126
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	385.0
3	47.0
4	71.0
5	126.0
6	332.0
7	875.0
8	1898.0
9	3344.0
10	5084.0
11	7015.0
12	10368.0
13	14455.0
14	18031.0
15	23968.0
16	30503.0
17	43711.0
18	42915.0
19	23642.0
20	17800.0
21	17773.0
22	19076.0
23	21036.0
24	24833.0
25	31086.0
26	40224.0
27	52608.0
28	69725.0
29	93073.0
30	120326.0
31	157593.0
32	203672.0
33	266474.0
34	360551.0
35	524250.0
36	866065.0
37	1574841.0
38	2193023.0
39	859552.0
40	2550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.722722264026984	20.92447837414499	15.341287739135186	23.01151162269284
2	15.513033959288322	20.62950921807093	39.745203631357754	24.112253191282996
3	18.07069702566222	25.281354531247977	30.7308631133038	25.917085329785998
4	12.934230658774403	17.556162685945534	37.81693480011829	31.692671855161773
5	12.999154047334605	37.6188692647961	35.42237577088244	13.959600916986858
6	30.61365556040595	38.03238630052771	17.298257215108787	14.055700923957545
7	26.7082970581698	33.031650540281994	22.06639346157209	18.193658939976114
8	25.0074487585467	36.32934219357835	20.029069208039726	18.634139839835225
9	24.709117347391462	17.054645811601056	21.618233687408296	36.61800315359919
10-11	23.887836096574137	27.23811785789331	28.356219458314136	20.51782658721841
12-13	24.554138336144188	24.613269923848303	28.893597431608477	21.938994308399028
14-15	21.746398523395722	26.660577647872397	26.613528095834372	24.97949573289751
16-17	20.81295498935089	29.98866316384518	27.44183995120175	21.756541895602176
18-19	20.72732171055784	28.337615320149386	30.22655203779565	20.708510931497123
20-21	21.713308745649726	27.41819894119788	30.14746669239346	20.721025620758937
22-23	21.42031778528487	27.26679573973631	30.224989316019922	21.087897158958896
24-25	21.290592505315516	27.38278585765206	30.140660457882646	21.18596117914978
26-27	21.078423965384548	27.482832338938596	30.147363371945474	21.291380323731378
28-29	21.074929109175226	27.460043353310365	30.335984280808777	21.129043256705636
30-31	21.33339274275205	27.64367393011386	30.006832083152524	21.016101243981566
32-33	21.110765992070153	27.501291505766574	30.227085458936575	21.160857043226695
34-35	21.29152884687535	27.373364324301708	30.11141431357575	21.22369251524719
36-37	21.510284065365166	27.47743384553154	29.791702102351557	21.220579986751737
38-39	21.342394794922473	27.581096542497445	29.90302342752413	21.173485235055956
40-41	21.300214222033834	27.462504040798148	29.924133086552445	21.313148650615577
42-43	21.31570583170313	27.51213014346948	29.76477421059626	21.407389814231127
44-45	21.42965537077124	27.61731681704312	29.562337165359597	21.39069064682604
46-47	21.54881613493444	27.727895526495814	29.238362985656153	21.484925352913592
48-49	21.61193201359542	27.75187878548363	28.938733686508456	21.697455514412493
50-51	21.630297223224215	27.825772278374732	28.77573276476091	21.768197733640143
52-53	21.6011673144213	27.94036757024273	28.726862192865436	21.73160292247053
54-55	21.70795545493866	28.096226466023523	28.589433081993427	21.60638499704439
56-57	21.590589883559147	27.902106716849406	28.63950475409669	21.86779864549476
58-59	21.565600003461242	27.654674430439975	28.9062687167812	21.87345684931759
60-61	21.633332261383686	27.825087780406854	28.738608436295387	21.802971521914074
62-63	21.463725288493293	27.858047003313096	28.793633032373783	21.884594675819823
64-65	21.48877403960092	27.752246864579565	28.842400283821267	21.916578811998242
66-67	21.399233956368548	27.720107747729177	28.78315892195961	22.09749937394266
68-69	21.675215787984374	27.85500550762563	28.605105502446694	21.8646732019433
70-71	21.629205900992407	27.695297925157508	28.604343514142826	22.07115265970726
72-73	21.54744068147068	27.776120345591398	28.651548043814586	22.024890929123337
74-75	21.618312051258307	27.820573968335637	28.47085349535013	22.090260485055925
76-77	21.61681390476257	27.778122179271055	28.404696121001678	22.200367794964706
78-79	21.641920629243128	27.826201058403328	28.510751703223967	22.021126609129578
80-81	21.526678200709917	27.84216743235976	28.477093007817768	22.154061359112557
82-83	21.472329607630446	27.764369041541265	28.62373930937058	22.139562041457705
84-85	21.532465674041294	27.689466777374527	28.46280741546353	22.31526013312065
86-87	21.577868553401366	27.76707334886498	28.400847434314347	22.254210663419304
88-89	21.492731174368966	27.65004095044818	28.445060177431063	22.41216769775179
90-91	21.361994271910664	27.642661092180496	28.434335163484047	22.561009472424786
92-93	21.417421976111388	27.727311883617062	28.401049328974732	22.454216811296817
94-95	21.32214108292707	27.569072529101817	28.4959783904069	22.612807997564207
96-97	21.284360990796603	27.65824462949998	28.413052162232216	22.644342217471202
98-99	21.25060692726553	27.610165007371595	28.414792836246743	22.724435229116132
100	21.35469645911589	27.50029317514102	28.370183855684473	22.774826510058617
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	430.0
1	335.5
2	471.5
3	738.0
4	538.5
5	385.5
6	990.0
7	2700.0
8	4798.5
9	5844.0
10	5019.0
11	3771.5
12	3739.5
13	4239.5
14	4829.0
15	5512.5
16	6202.5
17	7035.5
18	7943.5
19	9118.5
20	10868.5
21	13123.5
22	16859.0
23	22977.5
24	30532.5
25	39568.0
26	52268.0
27	68217.0
28	89624.0
29	113223.0
30	133590.0
31	154056.5
32	174640.5
33	194231.0
34	212220.5
35	229473.5
36	244528.0
37	256665.0
38	267711.5
39	275571.5
40	280901.0
41	282383.5
42	280057.5
43	278859.0
44	275496.0
45	269571.5
46	263162.0
47	254147.5
48	244760.5
49	240722.5
50	236323.0
51	225759.5
52	212595.0
53	202785.5
54	194680.0
55	183171.0
56	167971.0
57	149712.5
58	135077.5
59	124792.5
60	110149.5
61	92266.0
62	78191.5
63	64641.5
64	51626.0
65	41118.5
66	32069.5
67	24565.0
68	18807.0
69	14375.5
70	10693.0
71	8040.5
72	6040.5
73	4237.5
74	3018.0
75	2081.0
76	1298.0
77	851.0
78	573.0
79	385.5
80	261.0
81	125.5
82	70.5
83	45.0
84	20.0
85	12.0
86	9.0
87	3.5
88	3.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.5
97	1.5
98	1.5
99	3.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.058737674677746744
2	1.5498067197294656E-4
3	1.2915055997745548E-5
4	0.1194900980911418
5	0.0019501734556595777
6	0.0015627217757272112
7	0.0
8	0.0
9	2.5830111995491096E-5
10-11	0.0
12-13	6.457527998872774E-6
14-15	6.457527998872774E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.1623550397970992E-4
30-31	2.7121617595265654E-4
32-33	1.2915055997745548E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.1309842396280152E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.4852314397407378E-4
80-81	3.228763999436387E-4
82-83	2.8413123195040205E-4
84-85	0.0
86-87	0.0
88-89	8.394786398534606E-5
90-91	6.457527998872774E-5
92-93	6.199226878917862E-4
94-95	5.618049359019313E-4
96-97	0.0
98-99	5.811775198985496E-5
100	0.0011494399837993538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7742901.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.86222561526347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13390626276038	39.57426639897827
2	15.81432549350324	17.352181863555753
3	5.356719071144813	8.816445907161938
4	2.280311726206732	5.004119055851385
5	1.2912228409547633	3.541967941002085
6	0.7342559224677674	2.4169748446662034
7	0.47945517279644995	1.841278450365478
8	0.336216481479347	1.4756467569991991
9	0.24921538298679768	1.2305259511394389
>10	1.2224182553219944	11.832626936006948
>50	0.06991812124973484	2.616726727879255
>100	0.030362902955141997	2.928415276673723
>500	0.0011542640728375497	0.41553979685975545
>1k	4.2390173632271164E-4	0.3097466308946773
>5k	4.710018192607128E-5	0.19496436991441246
>10k+	4.710018192607128E-5	0.4485730920515896
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17399	0.22470905930477478	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17320	0.22368876988095288	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9567	0.12355834073043165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.2915055997745548E-5	0.0	0.0	0.0	0.0
3	1.2915055997745548E-5	0.0	0.0	0.0	0.0
4	1.2915055997745548E-5	0.0	0.0	1.2915055997745548E-5	0.0
5	1.2915055997745548E-5	1.2915055997745548E-5	0.0	1.2915055997745548E-5	0.0
6	1.2915055997745548E-5	1.2915055997745548E-5	0.0	1.2915055997745548E-5	0.0
7	1.2915055997745548E-5	1.2915055997745548E-5	0.0	1.2915055997745548E-5	0.0
8	1.2915055997745548E-5	1.2915055997745548E-5	0.0	1.2915055997745548E-5	0.0
9	7.749033598647328E-5	1.2915055997745548E-5	0.0	6.457527998872774E-5	0.0
10-11	7.749033598647328E-5	1.2915055997745548E-5	0.0	7.749033598647328E-5	0.0
12-13	7.749033598647328E-5	1.2915055997745548E-5	0.0	9.040539198421883E-5	0.0
14-15	1.1623550397970994E-4	1.2915055997745548E-5	0.0	9.68629199830916E-5	0.0
16-17	1.1623550397970994E-4	2.5830111995491096E-5	0.0	1.0332044798196438E-4	0.0
18-19	1.1623550397970994E-4	2.5830111995491096E-5	0.0	1.0977797598083717E-4	0.0
20-21	1.1623550397970994E-4	2.5830111995491096E-5	0.0	1.1623550397970994E-4	0.0
22-23	1.1623550397970994E-4	2.5830111995491096E-5	0.0	2.1309842396280155E-4	0.0
24-25	1.1623550397970994E-4	2.5830111995491096E-5	0.0	4.0682426392898475E-4	0.0
26-27	1.1623550397970994E-4	2.5830111995491096E-5	0.0	8.265635838557151E-4	0.0
28-29	1.1623550397970994E-4	2.5830111995491096E-5	0.0	0.0026411289515389643	0.0
30-31	1.1623550397970994E-4	2.5830111995491096E-5	0.0	0.008265635838557151	0.0
32-33	1.1623550397970994E-4	2.5830111995491096E-5	0.0	0.020244350276466146	0.0
34-35	1.291505599774555E-4	2.5830111995491096E-5	0.0	0.03998501336902022	0.0
36-37	1.291505599774555E-4	2.5830111995491096E-5	0.0	0.07174959359547539	0.0
38-39	1.5498067197294656E-4	2.5830111995491096E-5	0.0	0.1147502725399692	0.0
40-41	1.5498067197294656E-4	2.5830111995491096E-5	0.0	0.16002400134006622	0.0
42-43	1.5498067197294656E-4	2.5830111995491096E-5	0.0	0.2070606352838555	0.0
44-45	1.6143819997181934E-4	2.5830111995491096E-5	0.0	0.2666377886014557	0.0
46-47	1.8081078396843767E-4	2.5830111995491096E-5	1.2915055997745548E-5	0.3375737336690731	0.0
48-49	1.8726831196731045E-4	2.5830111995491096E-5	1.2915055997745548E-5	0.4131268112558846	0.0
50-51	1.9372583996618323E-4	2.5830111995491096E-5	1.2915055997745548E-5	0.4898422438824931	0.0
52-53	1.9372583996618323E-4	2.5830111995491096E-5	1.2915055997745548E-5	0.5672228018929856	0.0
54-55	1.9372583996618323E-4	2.5830111995491096E-5	1.2915055997745548E-5	0.6564400087254119	0.0
56-57	2.0018336796505599E-4	2.5830111995491096E-5	1.2915055997745548E-5	0.7596765088433909	0.0
58-59	2.0664089596392877E-4	3.874516799323664E-5	1.2915055997745548E-5	0.8763123795590309	0.0
60-61	2.195559519616743E-4	3.874516799323664E-5	1.2915055997745548E-5	0.9931290610586394	0.0
62-63	2.3247100795941987E-4	3.874516799323664E-5	1.2915055997745548E-5	1.1105785803021373	0.0
64-65	2.3247100795941987E-4	3.874516799323664E-5	1.2915055997745548E-5	1.2388702890557428	0.0
66-67	2.3247100795941987E-4	3.874516799323664E-5	1.2915055997745548E-5	1.387173877077855	0.0
68-69	2.3247100795941987E-4	3.874516799323664E-5	1.2915055997745548E-5	1.5541203484327126	0.0
70-71	2.3247100795941987E-4	3.874516799323664E-5	1.2915055997745548E-5	1.7311663935778077	0.0
72-73	2.3892853595829263E-4	3.874516799323664E-5	1.2915055997745548E-5	1.9121579883302138	0.0
74-75	2.58301119954911E-4	3.874516799323664E-5	1.2915055997745548E-5	2.0984835012096887	0.0
76-77	2.58301119954911E-4	3.874516799323664E-5	1.2915055997745548E-5	2.3016308745262273	0.0
78-79	2.58301119954911E-4	3.874516799323664E-5	1.2915055997745548E-5	2.5330041027258385	0.0
80-81	2.58301119954911E-4	3.874516799323664E-5	1.2915055997745548E-5	2.7811087859705297	0.0
82-83	2.8413123195040205E-4	3.874516799323664E-5	1.2915055997745548E-5	3.0374855625817765	0.0
84-85	2.8413123195040205E-4	3.874516799323664E-5	1.2915055997745548E-5	3.2940625225609885	0.0
86-87	2.8413123195040205E-4	3.874516799323664E-5	1.2915055997745548E-5	3.569224248120956	0.0
88	2.8413123195040205E-4	3.874516799323664E-5	1.2915055997745548E-5	3.793436077769818	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13420	0.0	55.999615	1
GTATCAA	23185	0.0	39.968937	1
ATCAACG	29295	0.0	31.180357	3
TCAACGC	29500	0.0	30.963678	4
TATCAAC	30550	0.0	30.176737	2
CAACGCA	30745	0.0	29.671644	5
GTGGTAT	5385	0.0	28.506018	1
AACGCAG	32280	0.0	28.332378	6
ACGCAGA	36995	0.0	24.555962	7
CGCAGAG	37475	0.0	24.04078	8
TGGTATC	6430	0.0	23.054123	2
GCAGAGT	40510	0.0	22.007626	9
GTACATG	24030	0.0	21.848707	1
GAGTACT	26390	0.0	21.120949	12-13
TACATGG	24385	0.0	20.68812	2
CAGAGTA	39600	0.0	20.032972	10-11
AGAGTAC	37745	0.0	19.803518	10-11
ACATGGG	24805	0.0	19.787645	3
GTACTTT	28975	0.0	19.114998	14-15
AGTACTT	28130	0.0	18.628302	12-13
>>END_MODULE