FastQCFastQC Report
Wed 25 May 2016
SRR2049500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8584076
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84650.041.345051
GTATCAA125650.035.5912551
TCAACGC137400.031.3509794
ATCAACG138800.031.0718213
CAACGCA149250.028.8618035
TATCAAC158500.027.823172
AACGCAG161500.026.672426
GTACATG236250.024.430461
TACATGG240400.023.1751212
ACGCAGA188850.022.2134957
CGCAGAG195400.021.9286698
ACATGGG248850.021.606963
GTGGTAT52600.019.4553321
TAGACCG4101.0913936E-1118.7168665
CATGGGG175000.018.5050984
GCAGAGT224300.018.1349899
TGGTATC54350.017.884742
GAGTACA179600.016.9513781
AGAGTAC191000.016.54702410-11
CAGAGTA209950.016.15260710-11
Produced by FastQC (version 0.11.5)