##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049500_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8584076 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.75572548518909 31.0 31.0 34.0 27.0 34.0 2 31.000145268984106 31.0 31.0 34.0 28.0 34.0 3 31.059148474454325 31.0 31.0 34.0 27.0 34.0 4 34.662036077033804 37.0 35.0 37.0 32.0 37.0 5 34.68878374329398 37.0 35.0 37.0 32.0 37.0 6 34.6524463436717 37.0 35.0 37.0 32.0 37.0 7 30.742434829328165 37.0 33.0 37.0 2.0 37.0 8 32.4813850669542 37.0 33.0 37.0 17.0 37.0 9 35.25317832693932 39.0 34.0 39.0 28.0 39.0 10-11 35.819889117943504 39.0 36.0 39.0 30.0 39.0 12-13 35.99511665553753 39.0 37.0 39.0 31.0 39.0 14-15 37.177562675353755 40.0 37.0 41.0 31.0 41.0 16-17 37.17975883484722 40.0 37.0 41.0 31.0 41.0 18-19 37.16336708808263 40.0 37.0 41.0 31.0 41.0 20-21 37.242288628385865 40.0 37.0 41.0 31.0 41.0 22-23 37.185788196656226 40.0 37.0 41.0 31.0 41.0 24-25 37.158111368072696 40.0 37.0 41.0 31.0 41.0 26-27 37.184500230426664 40.0 37.0 41.0 31.0 41.0 28-29 37.165077289623255 40.0 37.0 41.0 31.0 41.0 30-31 37.087371022810146 40.0 37.0 41.0 31.0 41.0 32-33 37.04454486423466 40.0 37.0 41.0 30.0 41.0 34-35 36.98672128485349 40.0 37.0 41.0 30.0 41.0 36-37 36.81599428989212 40.0 37.0 41.0 30.0 41.0 38-39 36.70892953417467 40.0 37.0 41.0 30.0 41.0 40-41 36.634090611499715 40.0 36.5 41.0 30.0 41.0 42-43 36.51060527656092 40.0 36.0 41.0 30.0 41.0 44-45 36.38519172011058 40.0 36.0 41.0 30.0 41.0 46-47 36.23112446814311 40.0 36.0 41.0 29.0 41.0 48-49 36.151512113825646 39.5 35.0 41.0 29.0 41.0 50-51 36.00998127230001 39.0 35.0 41.0 29.0 41.0 52-53 35.89249512702357 39.0 35.0 41.0 28.0 41.0 54-55 35.771989961412274 39.0 35.0 41.0 28.0 41.0 56-57 35.570938444627004 39.0 35.0 41.0 28.0 41.0 58-59 35.38775652731873 39.0 35.0 40.0 28.0 41.0 60-61 35.163968434109854 38.5 34.0 40.0 27.5 41.0 62-63 34.92538573749813 38.0 34.0 40.0 27.0 41.0 64-65 34.56193811657772 37.5 34.0 40.0 26.5 41.0 66-67 31.096403386922482 36.0 30.0 40.0 6.5 41.0 68-69 31.44358227956043 36.0 30.0 39.0 11.0 41.0 70-71 31.599646310214403 35.5 30.0 39.0 15.5 41.0 72-73 32.81665895082942 35.0 31.5 39.0 23.5 40.5 74-75 32.89689263002797 35.0 33.0 38.5 24.5 40.0 76-77 32.595668130151694 35.0 33.0 37.5 24.0 39.5 78-79 29.250222854504084 35.0 29.0 37.0 6.5 39.0 80-81 31.006832127301763 35.0 29.5 36.0 18.5 39.0 82-83 31.40074097666423 35.0 32.0 36.0 20.5 37.5 84-85 29.579577813616748 34.5 30.0 35.5 11.0 37.0 86-87 29.210883093299735 34.0 28.0 35.0 10.5 37.0 88-89 30.431574289416822 34.0 30.5 35.0 18.0 36.0 90-91 30.575412892430123 35.0 31.5 35.0 17.5 36.0 92-93 30.42394743476176 35.0 32.0 35.0 11.5 36.0 94-95 30.213754689497158 35.0 31.5 35.0 4.5 36.0 96-97 30.018483876424206 35.0 31.0 35.0 2.0 35.0 98-99 29.822391425704993 34.5 31.0 35.0 2.0 35.0 100 29.60237024928484 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.371415589293111 1101 2 0.3786021996794169 1101 3 0.4055622217031818 1101 4 0.38493811857401283 1101 5 0.38012107595529443 1101 6 0.4274783958442754 1101 7 -12.123357345689143 1101 8 -6.552115102479124 1101 9 -2.1713738469372856 1101 10-11 -0.0916288460785637 1101 12-13 0.42036961726357447 1101 14-15 0.639038432709917 1101 16-17 0.6825646149944546 1101 18-19 0.6272762775604548 1101 20-21 0.6775515358516344 1101 22-23 0.6542337835536145 1101 24-25 0.6672715410595842 1101 26-27 0.6513352131390562 1101 28-29 0.5937510388362313 1101 30-31 0.6707307126210935 1101 32-33 0.70832003250257 1101 34-35 0.7174719794344071 1101 36-37 0.7308715900106435 1101 38-39 0.6865353335309479 1101 40-41 0.6759605376656168 1101 42-43 0.6789022913967031 1101 44-45 0.6295338774378152 1101 46-47 0.5932110583136847 1101 48-49 0.5250138594128586 1101 50-51 0.6368984977476728 1101 52-53 0.6076130478238468 1101 54-55 0.5654583619036728 1101 56-57 0.5440080457701768 1101 58-59 0.5337447014543102 1101 60-61 0.5383521593235869 1101 62-63 0.5485279562185994 1101 64-65 0.41530346274929997 1101 66-67 -10.238478004084161 1101 68-69 -8.128775010362155 1101 70-71 -6.6498008156615 1101 72-73 -1.4694793282702427 1101 74-75 -0.15383974724559835 1101 76-77 0.1827816848899957 1101 78-79 3.2942176758408515 1101 80-81 1.24396880560381 1101 82-83 0.4930955431130535 1101 84-85 -5.274844688887718 1101 86-87 -5.799395074502463 1101 88-89 -1.2337396797341462 1101 90-91 0.07132859893615517 1101 92-93 0.436204905757954 1101 94-95 0.47179185239095744 1101 96-97 0.5937525696880925 1101 98-99 0.6007058192619823 1101 100 0.645899710550573 1102 1 0.2207557026717346 1102 2 0.24024360603486627 1102 3 0.2599463473724306 1102 4 0.2591786661648925 1102 5 0.24625620312690444 1102 6 0.2743831227766407 1102 7 -12.229213813263936 1102 8 -6.633903368647179 1102 9 -2.4256760781195794 1102 10-11 -0.28211757029996676 1102 12-13 0.2765691727297366 1102 14-15 0.4135168095438715 1102 16-17 0.44297669153385044 1102 18-19 0.46158377925645766 1102 20-21 0.4587413955354407 1102 22-23 0.4088918943333226 1102 24-25 0.46160924827419336 1102 26-27 0.440591732167384 1102 28-29 0.4111388627420709 1102 30-31 0.39649322787190044 1102 32-33 0.4475328750225529 1102 34-35 0.4635312242209082 1102 36-37 0.5754747885326239 1102 38-39 0.5597416965004598 1102 40-41 0.4675892289549921 1102 42-43 0.42232530065437146 1102 44-45 0.4310766371337067 1102 46-47 0.43138504613686735 1102 48-49 0.3655804751994083 1102 50-51 0.4294235044302326 1102 52-53 0.38363387283153827 1102 54-55 0.34996121831368754 1102 56-57 0.38347024660966156 1102 58-59 0.2722924993180129 1102 60-61 0.2318117986239372 1102 62-63 0.2579703359787686 1102 64-65 0.1696177550768354 1102 66-67 -10.345992817100296 1102 68-69 -6.790572668509544 1102 70-71 -6.327341383564729 1102 72-73 -1.1995398291802175 1102 74-75 -0.23504803084566106 1102 76-77 -0.0373920816146196 1102 78-79 3.1262486260352382 1102 80-81 1.0022054374990965 1102 82-83 0.34465532074027294 1102 84-85 -5.523242348512774 1102 86-87 -5.986103187950782 1102 88-89 -1.4334819902647205 1102 90-91 -0.10625446555080487 1102 92-93 0.26227285331549055 1102 94-95 0.27566460479998867 1102 96-97 0.37469405248501175 1102 98-99 0.36068223345125006 1102 100 0.46327541817451845 1103 1 0.2791114970913995 1103 2 0.2858888821788277 1103 3 0.29535617742979525 1103 4 0.314286795396697 1103 5 0.33112639470341776 1103 6 0.35641358987580674 1103 7 -12.173144871860242 1103 8 -6.584968132658496 1103 9 -2.2591722101062572 1103 10-11 -0.2558613235898264 1103 12-13 0.33426977759897625 1103 14-15 0.5386866241811816 1103 16-17 0.5029390965924776 1103 18-19 0.5763188282741396 1103 20-21 0.5545763377750177 1103 22-23 0.5564910047463059 1103 24-25 0.6300656467362913 1103 26-27 0.5932845860637457 1103 28-29 0.5313765201555043 1103 30-31 0.5499639317861664 1103 32-33 0.601186521215503 1103 34-35 0.5379410805931855 1103 36-37 0.6527182179742042 1103 38-39 0.6719964461228471 1103 40-41 0.6680262015289031 1103 42-43 0.6395340127017732 1103 44-45 0.5551477248322314 1103 46-47 0.5836698798214783 1103 48-49 0.5496866917110026 1103 50-51 0.5676756745555664 1103 52-53 0.5216215920373202 1103 54-55 0.5246021470121249 1103 56-57 0.5334913820129046 1103 58-59 0.5485043644638807 1103 60-61 0.4816719758274175 1103 62-63 0.40770969913094746 1103 64-65 0.31554115834025254 1103 66-67 -10.267276309281069 1103 68-69 -8.171914998759107 1103 70-71 -6.6854684092065 1103 72-73 -1.5013765919185609 1103 74-75 -0.2898040987972763 1103 76-77 0.031170280068145928 1103 78-79 3.1184726997087147 1103 80-81 1.0441488678390378 1103 82-83 0.3584076430707128 1103 84-85 -5.41628148673211 1103 86-87 -5.954662069466991 1103 88-89 -1.4010356084811875 1103 90-91 -0.09667863127080878 1103 92-93 0.3667530281323437 1103 94-95 0.45049569051416327 1103 96-97 0.5229696488416593 1103 98-99 0.5204170483377908 1103 100 0.5748922896403386 1104 1 0.18426128156567145 1104 2 0.18756836835187585 1104 3 0.18161209019211455 1104 4 0.23060755641706265 1104 5 0.17991514061983338 1104 6 0.26312814931388573 1104 7 -12.239901892419976 1104 8 -6.6497872826027695 1104 9 -2.4215522431788585 1104 10-11 -0.3703305284476244 1104 12-13 0.23641692741826148 1104 14-15 0.41503499375895814 1104 16-17 0.4620876600887982 1104 18-19 0.44826008922476035 1104 20-21 0.4214019398992974 1104 22-23 0.46127861148978155 1104 24-25 0.5398981046035303 1104 26-27 0.4893852814317796 1104 28-29 0.46301537502249346 1104 30-31 0.41836142552948274 1104 32-33 0.4623056424009988 1104 34-35 0.4444039975161189 1104 36-37 0.4926243079764916 1104 38-39 0.5250147685407995 1104 40-41 0.48575998857520375 1104 42-43 0.4620128716945686 1104 44-45 0.46352872165429204 1104 46-47 0.5072486224834805 1104 48-49 0.47922387856865356 1104 50-51 0.49456012738544786 1104 52-53 0.49567585492967225 1104 54-55 0.4096812044455973 1104 56-57 0.4248088308193516 1104 58-59 0.41898615217783686 1104 60-61 0.38703202713593043 1104 62-63 0.3461571098116849 1104 64-65 0.21105078706996494 1104 66-67 -10.415796203778008 1104 68-69 -8.373817444881485 1104 70-71 -6.820499933353332 1104 72-73 -1.6879923387742153 1104 74-75 -0.4190135765433851 1104 76-77 -0.09208268110826623 1104 78-79 3.037031399506919 1104 80-81 0.9762274183100637 1104 82-83 0.29000159727362274 1104 84-85 -5.523309820483359 1104 86-87 -6.0170679047813 1104 88-89 -1.448919719864051 1104 90-91 -0.11482446979634986 1104 92-93 0.24163127013241237 1104 94-95 0.3119204086869978 1104 96-97 0.3545787636478863 1104 98-99 0.35641822549163393 1104 100 0.44132637093064275 1105 1 0.05427122549141927 1105 2 0.07733498805771788 1105 3 0.1126827016005123 1105 4 0.11600834772567481 1105 5 0.10411647247507005 1105 6 0.1276682005714349 1105 7 -12.215337559597 1105 8 -6.707558496025772 1105 9 -2.533545248444959 1105 10-11 -0.4787383780608465 1105 12-13 0.05027868533416324 1105 14-15 0.18314228473236227 1105 16-17 0.24469939661070583 1105 18-19 0.26537972978620417 1105 20-21 0.2714362191810693 1105 22-23 0.2319154603973601 1105 24-25 0.31656770978542426 1105 26-27 0.2842978154726694 1105 28-29 0.29700519612963205 1105 30-31 0.28790443649249653 1105 32-33 0.27341624345226023 1105 34-35 0.30041715689718274 1105 36-37 0.38591753104561377 1105 38-39 0.38416285626055924 1105 40-41 0.3442005098647911 1105 42-43 0.2805885687596117 1105 44-45 0.24599043741792315 1105 46-47 0.21839507985907858 1105 48-49 0.21427882077554727 1105 50-51 0.27317554636286445 1105 52-53 0.26103134721878973 1105 54-55 0.2737166143079648 1105 56-57 0.23558948767102805 1105 58-59 0.2379964283827789 1105 60-61 0.19286369825825744 1105 62-63 0.09959903576941542 1105 64-65 -0.04694748380677538 1105 66-67 -10.402755834422415 1105 68-69 -8.470781081703006 1105 70-71 -6.861010464819124 1105 72-73 -1.8344292739423622 1105 74-75 -0.6046307405367415 1105 76-77 -0.29833033693841315 1105 78-79 2.8352611753419303 1105 80-81 0.7065060670608752 1105 82-83 0.06455718912425112 1105 84-85 -5.600466933532125 1105 86-87 -6.108070479639476 1105 88-89 -1.667391344345031 1105 90-91 -0.3494162260447382 1105 92-93 0.0077631502556592125 1105 94-95 0.05962966267496128 1105 96-97 0.10507830716130329 1105 98-99 0.11657967919724399 1105 100 0.2514226218725888 1106 1 0.11733148702575491 1106 2 0.17167573001163206 1106 3 0.13518699141160795 1106 4 0.10716286351209448 1106 5 0.13756120423744278 1106 6 0.1622042504364316 1106 7 -12.192547479493992 1106 8 -6.649241020449402 1106 9 -2.550844174020348 1106 10-11 -0.40398493302205907 1106 12-13 0.14990042432879136 1106 14-15 0.30246975783017405 1106 16-17 0.2972301081124513 1106 18-19 0.31089553410348003 1106 20-21 0.2958811054377861 1106 22-23 0.29253000892977354 1106 24-25 0.38353762260617685 1106 26-27 0.32176993201100856 1106 28-29 0.3036159408818975 1106 30-31 0.28374501363312277 1106 32-33 0.30390057180876795 1106 34-35 0.39695782889045006 1106 36-37 0.39328753691067675 1106 38-39 0.3948345622600016 1106 40-41 0.3660608771123748 1106 42-43 0.3441403903056397 1106 44-45 0.23678346801636962 1106 46-47 0.30922770739464056 1106 48-49 0.27823210231501605 1106 50-51 0.3068616999311402 1106 52-53 0.31605371909501656 1106 54-55 0.24984248075447368 1106 56-57 0.3070988796746974 1106 58-59 0.2412856452606107 1106 60-61 0.21734543998465483 1106 62-63 0.14301196426252005 1106 64-65 0.07219292967250368 1106 66-67 -10.380879367854128 1106 68-69 -8.343033436216093 1106 70-71 -6.799577118732877 1106 72-73 -1.7563314929189744 1106 74-75 -0.5096246871330266 1106 76-77 -0.2238543353811835 1106 78-79 2.8987216875727064 1106 80-81 0.8027229017358586 1106 82-83 0.15089620797482084 1106 84-85 -5.558362068192643 1106 86-87 -6.024430795008524 1106 88-89 -1.5289152534713217 1106 90-91 -0.2538549900062499 1106 92-93 0.08616332057671272 1106 94-95 0.17586901995955984 1106 96-97 0.23007822585159943 1106 98-99 0.27151861312855274 1106 100 0.35962290089909743 1107 1 0.10014513328641428 1107 2 0.13577946788511142 1107 3 0.11374366400581692 1107 4 0.0915317562353053 1107 5 0.1194992173954148 1107 6 0.08928197167757901 1107 7 -12.19545784447806 1107 8 -6.715762726408052 1107 9 -2.5007206346315343 1107 10-11 -0.47703031100563464 1107 12-13 0.18730483535989606 1107 14-15 0.27078953410943285 1107 16-17 0.3061617711697764 1107 18-19 0.3321284630727277 1107 20-21 0.23080416475430354 1107 22-23 0.34954578441087847 1107 24-25 0.44004720938011843 1107 26-27 0.3139540849298825 1107 28-29 0.31172933889000376 1107 30-31 0.35150842249861114 1107 32-33 0.3002621754145949 1107 34-35 0.31899581798612076 1107 36-37 0.40524884942131223 1107 38-39 0.3869899577914566 1107 40-41 0.3793092294314562 1107 42-43 0.39262241106965234 1107 44-45 0.32637784213237353 1107 46-47 0.2853398553367299 1107 48-49 0.2871358883131023 1107 50-51 0.31400482955653075 1107 52-53 0.3226032140800754 1107 54-55 0.2466100623755736 1107 56-57 0.22677348103274397 1107 58-59 0.20772181266382006 1107 60-61 0.14538653370438936 1107 62-63 0.10191325599741674 1107 64-65 0.017882842872147364 1107 66-67 -10.30548674148302 1107 68-69 -8.31758329507014 1107 70-71 -6.81826663285041 1107 72-73 -1.813419015147261 1107 74-75 -0.548415468336259 1107 76-77 -0.27221707167594644 1107 78-79 2.8888257717086 1107 80-81 0.7695895496221858 1107 82-83 0.09952098862638081 1107 84-85 -5.578794045233682 1107 86-87 -6.027288927186863 1107 88-89 -1.5612850676046968 1107 90-91 -0.2918581709254262 1107 92-93 0.06366374606867353 1107 94-95 0.1317402220770454 1107 96-97 0.19825450863649152 1107 98-99 0.25978090397724785 1107 100 0.36920057812383433 1108 1 0.09811593282665854 1108 2 0.11769647483798806 1108 3 0.14733621602227842 1108 4 0.0390960068474584 1108 5 0.05257144779766776 1108 6 0.08498220756747799 1108 7 -12.319847639750034 1108 8 -6.758608079897453 1108 9 -2.627547951381686 1108 10-11 -0.5569769778158644 1108 12-13 0.08193275471049333 1108 14-15 0.23706498379419116 1108 16-17 0.25942384179676736 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2216 22-23 -1.4272937674820767 2216 24-25 -1.4776692605340145 2216 26-27 -1.928712986195947 2216 28-29 -2.0789944323546337 2216 30-31 -1.9873012773589807 2216 32-33 -2.9440361090044647 2216 34-35 -2.046885787126918 2216 36-37 -1.6950649224131595 2216 38-39 -1.9337146121757627 2216 40-41 -1.9473382570233753 2216 42-43 -1.5687305680248613 2216 44-45 -1.6783126728789597 2216 46-47 -1.9348165882033044 2216 48-49 -1.9880334262971502 2216 50-51 -2.5103543554582544 2216 52-53 -2.2741489472485696 2216 54-55 -1.8745944991654184 2216 56-57 -1.937455897869647 2216 58-59 -2.29448654489493 2216 60-61 -2.406325413429421 2216 62-63 -2.2560600138863904 2216 64-65 -1.9476454397773963 2216 66-67 1.4710938090870158 2216 68-69 0.6615936410674443 2216 70-71 0.250325322625379 2216 72-73 -1.5633160571183282 2216 74-75 -2.3189058540049245 2216 76-77 -1.9660694504013954 2216 78-79 0.6091900798727785 2216 80-81 -0.8484534894671043 2216 82-83 -1.756965209204708 2216 84-85 0.07664898500806672 2216 86-87 0.02017920058852596 2216 88-89 -1.2247049488790829 2216 90-91 -1.467726810173133 2216 92-93 -1.534892155664604 2216 94-95 -1.5477417963426987 2216 96-97 -1.5359721633387018 2216 98-99 -1.5831459057668482 2216 100 -2.795055870050625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126089.0 3 24476.0 4 14410.0 5 13134.0 6 11599.0 7 12617.0 8 14321.0 9 16476.0 10 18778.0 11 21241.0 12 24176.0 13 26815.0 14 30650.0 15 33415.0 16 35803.0 17 36831.0 18 34972.0 19 32898.0 20 32048.0 21 32040.0 22 34323.0 23 39801.0 24 47590.0 25 59535.0 26 75414.0 27 93130.0 28 115643.0 29 146013.0 30 179793.0 31 221444.0 32 292440.0 33 408612.0 34 615142.0 35 834218.0 36 934732.0 37 1561592.0 38 1763631.0 39 567114.0 40 1120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.70592987118034 20.479925168334546 14.806379007984072 23.007765952501046 2 15.213800393785043 20.15590635040997 40.864675156464955 23.76561809934003 3 18.416889727972965 25.04658978609297 30.290857497871322 26.24566298806274 4 12.771295491819226 16.961008193705755 38.638773537361665 31.628922777113356 5 13.305543358965394 37.655889023080505 35.50258151558188 13.535986102372217 6 30.871254043086804 37.65866538176385 17.881371254742078 13.588709320407261 7 27.299908073356384 32.4887383604918 22.297710342952634 17.913643223199184 8 24.79700785500967 36.518514048570864 20.531446832483777 18.153031263935688 9 24.570169229629375 17.593355417636097 21.872359937167378 35.96411541556715 10-11 23.71501603667069 27.30043979107361 28.8561226624741 20.1284215097816 12-13 24.080163083365058 24.6544997970661 29.01924447080851 22.24609264876033 14-15 21.58065702120997 26.995759357209792 26.156565948390952 25.267017673189287 16-17 21.155112589562858 30.30875850632364 26.93059981558832 21.605529088525177 18-19 21.09397097602584 29.359968387977926 28.745918605566867 20.800142030429367 20-21 22.15346311900449 28.503284948973246 28.34161931590653 21.001632616115735 22-23 21.91207883061613 28.28512352406945 28.370426822875288 21.432370822439132 24-25 21.71368240448943 28.440859563685127 28.317351803502206 21.528106228323235 26-27 21.453635734828353 28.47857293676508 28.35454657978084 21.713244748625723 28-29 21.45047411043425 28.520961370798677 28.502333856317208 21.526230662449866 30-31 21.754216761361388 28.786284045015442 28.10311791274914 21.35638128087403 32-33 21.44650413702117 28.575513377970253 28.44254040184877 21.535442083159808 34-35 21.62346066609429 28.407422120276276 28.401201294956824 21.56791591867261 36-37 21.8378417986611 28.5684221734342 28.080949674944556 21.512786352960145 38-39 21.656735195304375 28.67746212157617 28.195068880743186 21.47073380237627 40-41 21.613764836192036 28.441471161252537 28.322215460347742 21.62254854220769 42-43 21.527917773779805 28.45255478619157 28.31186984688914 21.70765759313948 44-45 21.62455335840926 28.52616196710556 28.262254335475596 21.587030339009587 46-47 21.65796560301435 28.580157427625885 28.145442082533005 21.61643488682676 48-49 21.592763023273516 28.480567947920477 28.172478265298924 21.75419076350708 50-51 21.59564461894901 28.4450296643262 28.141804426070312 21.81752129065448 52-53 21.60353655599348 28.47106394615833 28.15304417520502 21.772355322643165 54-55 21.675199361233247 28.630856958861216 28.055341301375424 21.638602378530113 56-57 21.586653664119318 28.482929613247222 28.076945614488608 21.853471108144852 58-59 21.51843664397884 28.25412981244509 28.30874275370845 21.91869078986762 60-61 21.681321417701906 28.499507074966836 28.041763928954722 21.777407578376536 62-63 21.550718042919478 28.54460006933921 28.051804729977835 21.852877157763476 64-65 21.599550046021726 28.350968296907187 28.089475650539626 21.96000600653146 66-67 21.4862545520547 28.346790668138727 28.05224822524964 22.114706554556932 68-69 21.90019676539058 28.442154812301755 27.929032407870196 21.728616014437467 70-71 21.6233083208671 28.29162498472423 28.05921187140553 22.025854823003144 72-73 21.514706111918688 28.35674632122947 28.180085885548223 21.94846168130362 74-75 21.54686210969357 28.388921356195883 28.045107040274537 22.019109493836012 76-77 21.540205655425165 28.36072730017926 27.939073447317618 22.159993597077957 78-79 21.710031393892265 28.350397354727836 28.01775605450998 21.921815196869918 80-81 21.477273223723817 28.30126802081049 28.089621521840495 22.131837233625195 82-83 21.462365538785015 28.28097364051082 28.23447855016402 22.022182270540146 84-85 21.580489069027742 28.168616344903352 28.057049254147387 22.193845331921523 86-87 21.62239240223414 28.32470262395273 27.985062303215486 22.067842670597642 88-89 21.481707182817967 28.171138834449387 28.093243396420505 22.25391058631214 90-91 21.429646542612154 28.095261228496867 28.096187390979317 22.378904837911666 92-93 21.529344606539663 28.194722019158235 28.00568514435665 22.270248229945448 94-95 21.50411106986375 27.999795426560375 28.130688643011137 22.365404860564738 96-97 21.46946231398142 28.055248129620615 28.07287431469905 22.402415241698918 98-99 21.485700245665896 28.06601512254344 28.05306602386013 22.395218607930534 100 21.552702148218376 27.927211705887682 27.962313617934154 22.557772527959784 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1475.0 1 1233.5 2 1121.5 3 1290.0 4 1246.0 5 1107.0 6 1031.5 7 1009.5 8 1057.0 9 1116.5 10 1081.5 11 1022.0 12 1034.0 13 1136.5 14 1283.5 15 1507.0 16 1835.0 17 2353.0 18 3264.5 19 4664.5 20 6803.0 21 10105.0 22 15492.5 23 23495.5 24 33682.0 25 45765.0 26 61917.5 27 82749.5 28 107757.0 29 134197.5 30 158093.0 31 181640.0 32 204535.5 33 225803.0 34 245908.0 35 264489.0 36 280215.0 37 293322.5 38 304581.5 39 310673.0 40 314791.5 41 316554.0 42 313543.5 43 309467.0 44 303993.0 45 297781.0 46 291927.5 47 284276.0 48 275175.5 49 268481.0 50 261011.5 51 249291.5 52 235920.5 53 224677.5 54 215186.5 55 202117.0 56 183036.5 57 162804.0 58 146112.5 59 131127.0 60 113744.5 61 94924.0 62 78948.0 63 64578.0 64 51780.0 65 41217.5 66 32432.5 67 25166.5 68 19159.5 69 14554.5 70 10673.0 71 7852.0 72 5900.0 73 4291.5 74 3116.5 75 2145.5 76 1377.0 77 895.5 78 651.0 79 472.0 80 316.5 81 221.5 82 176.5 83 129.5 84 102.5 85 87.0 86 75.0 87 64.0 88 46.5 89 39.0 90 35.5 91 28.5 92 21.5 93 23.0 94 26.5 95 20.5 96 15.0 97 17.0 98 19.5 99 25.5 100 45.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.01879060716610617 2 0.00238814288223916 3 0.0013163909546001222 4 0.0019804111706373524 5 0.0019221637832656654 6 0.003355049512609161 7 11.55806402459624 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.446390269610847E-4 18-19 0.0 20-21 5.824738737168683E-6 22-23 0.0 24-25 0.0 26-27 1.5726794590355443E-4 28-29 0.0 30-31 0.0 32-33 1.1649477474337366E-5 34-35 6.98968648460242E-5 36-37 2.3298954948674732E-5 38-39 5.824738737168683E-6 40-41 0.0 42-43 1.747421621150605E-5 44-45 1.397937296920484E-4 46-47 7.921644682549409E-4 48-49 5.766491349796996E-4 50-51 3.2618536928144624E-4 52-53 2.1551533327524128E-4 54-55 4.077317116018078E-4 56-57 0.0013629888644974717 58-59 3.49484324230121E-4 60-61 0.001322215693337291 62-63 0.0021901017651754246 64-65 0.001013504540267351 66-67 5.7793931460998245 68-69 5.429035111059129 70-71 0.0025978334767772326 72-73 0.0010950508825877123 74-75 0.0026502561254117506 76-77 0.005766491349796996 78-79 5.383386633575937 80-81 0.004933553710381874 82-83 0.004356904575402175 84-85 5.779509640874568 86-87 0.0033725237288206675 88-89 0.0015493805040868696 90-91 0.0031337094405967515 92-93 0.0035064927197755473 94-95 0.004211286106972957 96-97 0.0029822662334303657 98-99 0.00544030598051555 100 0.005801439782220009 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8584076.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.41862339160754 #Duplication Level Percentage of deduplicated Percentage of total 1 90.82372511095454 78.4886129538647 2 6.54187285571047 11.306792931868467 3 1.407813415959606 3.649838921983972 4 0.5266021696707883 1.8203293831193308 5 0.24627901757732026 1.0641546834634767 6 0.14075406874958035 0.7298263714863853 7 0.08928331953921953 0.540101909648866 8 0.05455090561432636 0.3771371334364479 9 0.04098791983779682 0.3187907647261167 >10 0.12680682450630448 1.6158328447203 >50 0.0011620919101764968 0.06988973713897167 >100 1.6229996987982088E-4 0.018692364542997348 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.3298954948674732E-5 0.0 0.0 0.0 3 0.0 2.3298954948674732E-5 0.0 1.1649477474337366E-5 0.0 4 0.0 2.3298954948674732E-5 0.0 1.1649477474337366E-5 0.0 5 0.0 2.3298954948674732E-5 0.0 1.1649477474337366E-5 0.0 6 0.0 2.3298954948674732E-5 0.0 1.1649477474337366E-5 0.0 7 0.0 2.3298954948674732E-5 0.0 4.6597909897349463E-5 0.0 8 0.0 2.3298954948674732E-5 0.0 4.6597909897349463E-5 0.0 9 0.0 2.3298954948674732E-5 0.0 5.824738737168683E-5 0.0 10-11 5.824738737168683E-6 2.3298954948674732E-5 0.0 5.824738737168683E-5 0.0 12-13 1.1649477474337366E-5 3.4948432423012096E-5 0.0 9.319581979469893E-5 0.0 14-15 1.1649477474337366E-5 6.407212610885551E-5 0.0 1.2231951348054234E-4 0.0 16-17 1.1649477474337366E-5 1.1067003600620498E-4 0.0 1.397937296920484E-4 0.0 18-19 1.7474216211506048E-5 1.2231951348054234E-4 0.0 1.5726794590355443E-4 0.0 20-21 2.3298954948674732E-5 1.2814425221771103E-4 0.0 1.8639163958939785E-4 0.0 22-23 2.3298954948674732E-5 1.2814425221771103E-4 0.0 2.6793798190975944E-4 0.0 24-25 2.3298954948674732E-5 1.2814425221771103E-4 0.0 4.6015436023632596E-4 0.0 26-27 2.3298954948674732E-5 1.3396899095487972E-4 0.0 9.319581979469893E-4 0.0 28-29 2.3298954948674732E-5 1.5144320716638576E-4 0.0 0.0029531425397445223 0.0 30-31 2.3298954948674732E-5 1.5144320716638576E-4 0.0 0.008655561763432663 0.0 32-33 2.3298954948674732E-5 1.5144320716638576E-4 0.0 0.021487461201415273 0.0 34-35 2.3298954948674732E-5 1.8639163958939785E-4 0.0 0.042025489988672046 0.0 36-37 2.3298954948674732E-5 2.038658558009039E-4 0.0 0.07411397569173432 0.0 38-39 2.3298954948674732E-5 2.2716481074957863E-4 0.0 0.1199663190307262 0.0 40-41 2.3298954948674732E-5 2.5628850443542206E-4 0.0 0.1681369083871112 0.0 42-43 2.3298954948674732E-5 2.6793798190975944E-4 0.0 0.21730352806755204 0.0 44-45 2.3298954948674732E-5 2.737627206469281E-4 0.0 0.278731222789733 0.0 46-47 4.6597909897349463E-5 2.7958745938409677E-4 0.0 0.3526529821031408 0.0 48-49 5.242264863451815E-5 2.7958745938409677E-4 0.0 0.4300346362264267 0.0 50-51 5.824738737168683E-5 2.9123693685843415E-4 0.0 0.5084763927998773 0.0 52-53 5.824738737168683E-5 3.145358918071089E-4 0.0 0.5867958298598475 0.0 54-55 5.824738737168683E-5 3.145358918071089E-4 0.0 0.6723612419088554 0.0 56-57 5.824738737168683E-5 3.145358918071089E-4 0.0 0.7665996899375076 0.0 58-59 5.824738737168683E-5 3.145358918071089E-4 0.0 0.86927235965758 0.0 60-61 5.824738737168683E-5 3.145358918071089E-4 0.0 0.9770882736825723 0.0 62-63 8.154634232036156E-5 3.145358918071089E-4 0.0 1.0852070741218973 0.0 64-65 9.319581979469893E-5 3.145358918071089E-4 0.0 1.1985914383796228 0.0 66-67 9.319581979469893E-5 3.203606305442776E-4 0.0 1.3258794540029701 0.0 68-69 9.319581979469893E-5 3.2618536928144624E-4 0.0 1.4560332410850045 0.0 70-71 9.319581979469893E-5 3.2618536928144624E-4 0.0 1.6079366026116264 0.0 72-73 9.319581979469893E-5 3.49484324230121E-4 0.0 1.770318669126415 0.0 74-75 9.319581979469893E-5 3.611338017044583E-4 0.0 1.9180806414109102 0.0 76-77 9.319581979469893E-5 3.611338017044583E-4 0.0 2.0739855984499673 0.0 78-79 9.319581979469893E-5 3.611338017044583E-4 0.0 2.257715332436479 0.0 80-81 9.319581979469893E-5 3.844327566531331E-4 0.0 2.476824529512553 0.0 82-83 9.319581979469893E-5 3.960822341274704E-4 0.0 2.7027719698660637 0.0 84-85 9.319581979469893E-5 4.193811890761452E-4 0.0 2.93173080014669 0.0 86-87 9.319581979469893E-5 4.193811890761452E-4 0.0 3.2036703775688844 0.0 88 1.048452972690363E-4 4.193811890761452E-4 0.0 3.4302701886609577 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8465 0.0 41.34505 1 GTATCAA 12565 0.0 35.591255 1 TCAACGC 13740 0.0 31.350979 4 ATCAACG 13880 0.0 31.071821 3 CAACGCA 14925 0.0 28.861803 5 TATCAAC 15850 0.0 27.82317 2 AACGCAG 16150 0.0 26.67242 6 GTACATG 23625 0.0 24.43046 1 TACATGG 24040 0.0 23.175121 2 ACGCAGA 18885 0.0 22.213495 7 CGCAGAG 19540 0.0 21.928669 8 ACATGGG 24885 0.0 21.60696 3 GTGGTAT 5260 0.0 19.455332 1 TAGACCG 410 1.0913936E-11 18.716866 5 CATGGGG 17500 0.0 18.505098 4 GCAGAGT 22430 0.0 18.134989 9 TGGTATC 5435 0.0 17.88474 2 GAGTACA 17960 0.0 16.951378 1 AGAGTAC 19100 0.0 16.547024 10-11 CAGAGTA 20995 0.0 16.152607 10-11 >>END_MODULE