FastQCFastQC Report
Wed 25 May 2016
SRR2049500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8584076
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221040.2575000500927531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209890.24451088270886698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121220.14121496594391755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152400.055.2329751
GTATCAA267400.041.0037841
ATCAACG332650.032.4440843
TCAACGC335050.032.267854
TATCAAC345400.031.4235252
GTGGTAT62450.031.3600481
CAACGCA348200.031.111525
AACGCAG364900.029.7130876
ACGCAGA411600.026.2505047
TGGTATC72800.025.7852382
CGCAGAG416600.025.7323848
GTACATG242000.023.2470781
GCAGAGT453400.023.1566499
TACATGG247250.021.7490122
GAGTACT306150.021.35339412-13
CAGAGTA437450.020.73494710-11
AGAGTAC417650.020.49139410-11
ACATGGG256700.020.1419473
GTACTTT333000.019.41995614-15
AGTACTT326900.019.17132612-13
Produced by FastQC (version 0.11.5)