##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049500_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8584076
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.858388951821954	33.0	31.0	34.0	30.0	34.0
2	31.94947912856317	33.0	31.0	34.0	30.0	34.0
3	31.97982706583679	33.0	31.0	34.0	30.0	34.0
4	35.625684465049005	37.0	35.0	37.0	33.0	37.0
5	35.74088964263597	37.0	35.0	37.0	33.0	37.0
6	35.80733616524365	37.0	35.0	37.0	35.0	37.0
7	35.79391771461483	37.0	35.0	37.0	33.0	37.0
8	35.770527078278434	37.0	35.0	37.0	33.0	37.0
9	37.48756290135362	39.0	37.0	39.0	34.0	39.0
10-11	37.46197097975367	39.0	37.0	39.0	34.5	39.0
12-13	37.45289859968621	39.0	37.0	39.0	34.0	39.0
14-15	38.73490198595633	40.0	38.0	41.0	34.5	41.0
16-17	38.740458553722036	40.0	38.0	41.0	34.5	41.0
18-19	38.814980202877976	40.0	38.0	41.0	35.0	41.0
20-21	38.84712041226103	40.0	38.0	41.0	35.0	41.0
22-23	38.82739551700148	40.0	38.0	41.0	35.0	41.0
24-25	38.79797155803374	40.0	38.0	41.0	35.0	41.0
26-27	38.71275161123923	40.0	38.0	41.0	34.5	41.0
28-29	38.634491470019604	40.0	38.0	41.0	34.0	41.0
30-31	38.53136109232956	40.0	38.0	41.0	34.0	41.0
32-33	38.40587251324429	40.0	38.0	41.0	34.0	41.0
34-35	38.32215523254919	40.0	38.0	41.0	34.0	41.0
36-37	38.200413067172285	40.0	38.0	41.0	34.0	41.0
38-39	38.12242703815763	40.0	38.0	41.0	33.5	41.0
40-41	38.01176486554872	40.0	38.0	41.0	33.0	41.0
42-43	37.884437591186284	40.0	37.0	41.0	33.0	41.0
44-45	37.712424086180036	40.0	37.0	41.0	33.0	41.0
46-47	37.48984363605355	40.0	37.0	41.0	33.0	41.0
48-49	37.28264707814796	40.0	37.0	41.0	32.0	41.0
50-51	37.078138462427404	40.0	37.0	41.0	31.0	41.0
52-53	36.98303434172764	40.0	36.5	41.0	31.0	41.0
54-55	36.85823675139875	40.0	36.0	41.0	31.0	41.0
56-57	36.689766376718936	39.0	36.0	41.0	31.0	41.0
58-59	36.51981401376223	39.0	35.0	41.0	31.0	41.0
60-61	36.337075592061396	39.0	35.0	41.0	31.0	41.0
62-63	36.14500582240884	39.0	35.0	41.0	30.0	41.0
64-65	35.873719838920344	38.0	35.0	40.0	30.0	41.0
66-67	35.62200707449468	38.0	35.0	40.0	30.0	41.0
68-69	35.31716372268838	37.0	35.0	40.0	29.0	41.0
70-71	35.027367826193526	37.0	34.5	39.5	29.0	41.0
72-73	34.70459930690269	36.0	34.0	39.0	29.0	41.0
74-75	34.36827277624289	36.0	34.0	39.0	29.0	40.5
76-77	34.07090815598558	35.5	34.0	38.5	29.0	40.0
78-79	33.74397226911784	35.0	34.0	37.0	29.0	39.0
80-81	33.43098634028869	35.0	34.0	37.0	28.5	39.0
82-83	33.13364268909082	35.0	34.0	36.5	28.0	39.0
84-85	32.85638797932357	35.0	33.5	36.0	28.0	37.0
86-87	32.57890103722288	35.0	33.0	36.0	27.0	37.0
88-89	32.37283133327338	35.0	33.0	35.0	27.0	37.0
90-91	32.22639157668222	35.0	33.0	35.0	27.0	36.0
92-93	32.071113711015606	35.0	33.0	35.0	27.0	36.0
94-95	31.891891625843016	35.0	33.0	35.0	26.0	36.0
96-97	31.729933600308293	35.0	33.0	35.0	26.0	36.0
98-99	31.55348793510216	35.0	33.0	35.0	25.5	35.0
100	31.443774728928307	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3648182419147723
1101	2	0.391845221812396
1101	3	0.4628410469760915
1101	4	0.38735163081971535
1101	5	0.3177453829660877
1101	6	0.2876393830527988
1101	7	0.2918753136750212
1101	8	0.33517520527608724
1101	9	0.4053090544127471
1101	10-11	0.358072642888672
1101	12-13	0.3212070490145891
1101	14-15	0.4888217915111852
1101	16-17	0.4996377153428
1101	18-19	0.4955341149422807
1101	20-21	0.44471324698631065
1101	22-23	0.4770327050493819
1101	24-25	0.4978497742825425
1101	26-27	0.45930813478553745
1101	28-29	0.466919251509907
1101	30-31	0.516399106786281
1101	32-33	0.5582241092818236
1101	34-35	0.5356486240228477
1101	36-37	0.5419285110908874
1101	38-39	0.5438744330649854
1101	40-41	0.5763323704007277
1101	42-43	0.5031970758996138
1101	44-45	0.5426498560638251
1101	46-47	0.5692630036615043
1101	48-49	0.5362495999429697
1101	50-51	0.5967877774144412
1101	52-53	0.5580769010670039
1101	54-55	0.5292328490615645
1101	56-57	0.5009766494361685
1101	58-59	0.49618387548362364
1101	60-61	0.521356367839914
1101	62-63	0.5098198736362605
1101	64-65	0.5229215292526348
1101	66-67	0.5080212318513162
1101	68-69	0.4794139462069964
1101	70-71	0.4982066319650542
1101	72-73	0.48414642877195035
1101	74-75	0.5076848211651637
1101	76-77	0.44232228720899514
1101	78-79	0.49350974516237045
1101	80-81	0.49101243132741246
1101	82-83	0.4520831033044601
1101	84-85	0.4621766306327757
1101	86-87	0.5282373984344062
1101	88-89	0.5185692046460133
1101	90-91	0.5285579867411911
1101	92-93	0.5197844116222328
1101	94-95	0.45536568199795724
1101	96-97	0.4731205267982084
1101	98-99	0.5769317490693737
1101	100	0.6426200173820753
1102	1	0.26146936224767714
1102	2	0.3356481419824462
1102	3	0.3967978546779314
1102	4	0.3596583040322088
1102	5	0.2899387779025133
1102	6	0.2504240371077273
1102	7	0.2725362390711439
1102	8	0.29967755939109253
1102	9	0.3337616792267184
1102	10-11	0.3034838444092074
1102	12-13	0.2946603167220587
1102	14-15	0.3696187225162575
1102	16-17	0.3722043000322799
1102	18-19	0.40568404145613357
1102	20-21	0.3614281444497607
1102	22-23	0.34447173161819933
1102	24-25	0.3474511665274136
1102	26-27	0.33011295143628416
1102	28-29	0.3382577359309309
1102	30-31	0.38589927969486126
1102	32-33	0.38097664788411123
1102	34-35	0.4154680784654019
1102	36-37	0.4202272274211296
1102	38-39	0.3919431363256365
1102	40-41	0.3978469668400493
1102	42-43	0.38346466736251017
1102	44-45	0.4138456605827443
1102	46-47	0.3959150690368318
1102	48-49	0.36113111676582577
1102	50-51	0.4157396217137901
1102	52-53	0.36798063961953176
1102	54-55	0.33197902158462966
1102	56-57	0.3353222919194536
1102	58-59	0.36541389252305123
1102	60-61	0.3584717592837663
1102	62-63	0.3409846544084729
1102	64-65	0.34985596326816193
1102	66-67	0.3946708921653581
1102	68-69	0.370353142486465
1102	70-71	0.33525157253655635
1102	72-73	0.3746913979042361
1102	74-75	0.3646842166467721
1102	76-77	0.33272501753248207
1102	78-79	0.32218210060946717
1102	80-81	0.26889487687224545
1102	82-83	0.27455150889328195
1102	84-85	0.33090461456286135
1102	86-87	0.3661526850303787
1102	88-89	0.3697876036184411
1102	90-91	0.33719853053866444
1102	92-93	0.3679680610430367
1102	94-95	0.37203839991349597
1102	96-97	0.4265403563244625
1102	98-99	0.44086198861832315
1102	100	0.5050533240733728
1103	1	0.2977838169793152
1103	2	0.29199627979720333
1103	3	0.42680726523908064
1103	4	0.31645437167853885
1103	5	0.23648427435234964
1103	6	0.27248769676739215
1103	7	0.2728609618642537
1103	8	0.2948455935982466
1103	9	0.3212713793711117
1103	10-11	0.2777970054172556
1103	12-13	0.26534148369842114
1103	14-15	0.4060915039567803
1103	16-17	0.38000178113105676
1103	18-19	0.37768757234447037
1103	20-21	0.3841196352974592
1103	22-23	0.3721884358849792
1103	24-25	0.39334657343103174
1103	26-27	0.3882807353735487
1103	28-29	0.41636172648745173
1103	30-31	0.4034506977146535
1103	32-33	0.3743216194031902
1103	34-35	0.37781809242979847
1103	36-37	0.4059204038540116
1103	38-39	0.4201178670807906
1103	40-41	0.41823017908902926
1103	42-43	0.42378113474740786
1103	44-45	0.4252934209971855
1103	46-47	0.4452756863193983
1103	48-49	0.4611985059874115
1103	50-51	0.4407567919929605
1103	52-53	0.41833824213577486
1103	54-55	0.3897465764311363
1103	56-57	0.4712342537626739
1103	58-59	0.4173239553997874
1103	60-61	0.3873043826313278
1103	62-63	0.4675645327572724
1103	64-65	0.48257449964089005
1103	66-67	0.4780851776309234
1103	68-69	0.41420461239672335
1103	70-71	0.4841523666028351
1103	72-73	0.5063694419925966
1103	74-75	0.46917175526549926
1103	76-77	0.47310351774328296
1103	78-79	0.4214410616507678
1103	80-81	0.3944745700626626
1103	82-83	0.47225371849495446
1103	84-85	0.4965390868865356
1103	86-87	0.4655911896725726
1103	88-89	0.48147055054794663
1103	90-91	0.46088001249891875
1103	92-93	0.4731203194226836
1103	94-95	0.4702773799889748
1103	96-97	0.4571162827463944
1103	98-99	0.49306421853255955
1103	100	0.4797097813504827
1104	1	0.251576959280154
1104	2	0.24182730290102228
1104	3	0.30401151661014225
1104	4	0.25043729907353907
1104	5	0.20243253674523487
1104	6	0.24441384157395873
1104	7	0.18467434277660288
1104	8	0.20731494529858452
1104	9	0.24789041367053244
1104	10-11	0.245679257768451
1104	12-13	0.24189017711736938
1104	14-15	0.3426495141285528
1104	16-17	0.3359181986353974
1104	18-19	0.33259755951733894
1104	20-21	0.3380112834119089
1104	22-23	0.36753531201313194
1104	24-25	0.3263511242795829
1104	26-27	0.3323947756292327
1104	28-29	0.3040037864850831
1104	30-31	0.34615147694552917
1104	32-33	0.35156961232222983
1104	34-35	0.3141263464287789
1104	36-37	0.326886140001335
1104	38-39	0.35642520331388994
1104	40-41	0.3916831687701645
1104	42-43	0.41356287541078274
1104	44-45	0.4196424760095354
1104	46-47	0.43850310438799056
1104	48-49	0.4066156848881235
1104	50-51	0.49016851040891396
1104	52-53	0.4402489849888269
1104	54-55	0.3926017048307102
1104	56-57	0.36518491898733885
1104	58-59	0.39485605243577737
1104	60-61	0.37101910644159375
1104	62-63	0.3857388124984169
1104	64-65	0.41227338795646773
1104	66-67	0.4372534778573609
1104	68-69	0.3996595410091359
1104	70-71	0.3788669464921668
1104	72-73	0.43178110604124953
1104	74-75	0.48079974111679036
1104	76-77	0.42064436880505696
1104	78-79	0.3826966600226953
1104	80-81	0.3545973976250636
1104	82-83	0.36334310406561343
1104	84-85	0.37676061588323506
1104	86-87	0.38559453872415617
1104	88-89	0.3710497317051491
1104	90-91	0.35497067354187095
1104	92-93	0.36106244758268957
1104	94-95	0.3431821172869043
1104	96-97	0.403587961951839
1104	98-99	0.4340289594278772
1104	100	0.4786086972816044
1105	1	0.18363052053566875
1105	2	0.20651975270583733
1105	3	0.26537584424965743
1105	4	0.20235976285901813
1105	5	0.14201064485162362
1105	6	0.20596970042155505
1105	7	0.17324364487291888
1105	8	0.208139909711214
1105	9	0.23924139879645168
1105	10-11	0.18171285879966348
1105	12-13	0.2171522840364517
1105	14-15	0.2668817816908202
1105	16-17	0.25064148634615435
1105	18-19	0.2358143045797334
1105	20-21	0.24462741095822338
1105	22-23	0.27570936812259106
1105	24-25	0.264981674308018
1105	26-27	0.27436613751429917
1105	28-29	0.28016453259981233
1105	30-31	0.2615757539632071
1105	32-33	0.2884237767170532
1105	34-35	0.24952469271016042
1105	36-37	0.30307788294877724
1105	38-39	0.2839460814014103
1105	40-41	0.29349009279387417
1105	42-43	0.23363870059524317
1105	44-45	0.2912140282644131
1105	46-47	0.29776207409263833
1105	48-49	0.37583433242620146
1105	50-51	0.4150613876533882
1105	52-53	0.3967479284925588
1105	54-55	0.32898492030558657
1105	56-57	0.36569185870812504
1105	58-59	0.3546518523191011
1105	60-61	0.36385702265246067
1105	62-63	0.32755755578375556
1105	64-65	0.34758989306388344
1105	66-67	0.34804512510643093
1105	68-69	0.32830176359757246
1105	70-71	0.3275591197643948
1105	72-73	0.27650872878528787
1105	74-75	0.3060104413383513
1105	76-77	0.2934149929765013
1105	78-79	0.25209106223064737
1105	80-81	0.2830457601169698
1105	82-83	0.2782331915340208
1105	84-85	0.291389727257652
1105	86-87	0.3532823119253834
1105	88-89	0.2726023667843478
1105	90-91	0.2755901872063973
1105	92-93	0.29208389560606207
1105	94-95	0.29549549202595315
1105	96-97	0.3423769558573575
1105	98-99	0.38518810404976733
1105	100	0.40466189276104103
1106	1	0.16420586371848955
1106	2	0.22736259853494545
1106	3	0.3080655126086178
1106	4	0.25085810751769344
1106	5	0.20442772004483345
1106	6	0.20390571133122393
1106	7	0.18939313512850475
1106	8	0.21014434462020404
1106	9	0.17529570123450355
1106	10-11	0.1743927356966566
1106	12-13	0.12093944979874038
1106	14-15	0.20351967489462197
1106	16-17	0.221592368817781
1106	18-19	0.2321837939300977
1106	20-21	0.22900219382437115
1106	22-23	0.2101815404955616
1106	24-25	0.2358454489583579
1106	26-27	0.22119939492505836
1106	28-29	0.2745198091801129
1106	30-31	0.2623307544476958
1106	32-33	0.2797203656822802
1106	34-35	0.2621815913083978
1106	36-37	0.280258708756584
1106	38-39	0.2765170239967958
1106	40-41	0.306108829053791
1106	42-43	0.33422558869111185
1106	44-45	0.34030291788577927
1106	46-47	0.35857538197029726
1106	48-49	0.36871910976836375
1106	50-51	0.4045543293623055
1106	52-53	0.3989604364033994
1106	54-55	0.31427681450192324
1106	56-57	0.3615048171610411
1106	58-59	0.3560553050565858
1106	60-61	0.33310420966502363
1106	62-63	0.34324002973032464
1106	64-65	0.32198127576366886
1106	66-67	0.3258876876939425
1106	68-69	0.29785145521974243
1106	70-71	0.3387165699144248
1106	72-73	0.3373643827549486
1106	74-75	0.39179765708096426
1106	76-77	0.3579776537619992
1106	78-79	0.33461910724729904
1106	80-81	0.32002523448542064
1106	82-83	0.3220778922768943
1106	84-85	0.31388386666141344
1106	86-87	0.39310787713099415
1106	88-89	0.33265002704183644
1106	90-91	0.33851281359321916
1106	92-93	0.33048581961472934
1106	94-95	0.32462729708463556
1106	96-97	0.36373687520380926
1106	98-99	0.39636777096668396
1106	100	0.3972694040246232
1107	1	0.14484803378615752
1107	2	0.17667095402320143
1107	3	0.2344278204769914
1107	4	0.19610404186868635
1107	5	0.1818936660161583
1107	6	0.16525901050782466
1107	7	0.14210924102611955
1107	8	0.2131902801449712
1107	9	0.17348457271245366
1107	10-11	0.16947404867680405
1107	12-13	0.1582838517917935
1107	14-15	0.1961263853429429
1107	16-17	0.18787345173882386
1107	18-19	0.18308246228698977
1107	20-21	0.2362511095534856
1107	22-23	0.20088351419136075
1107	24-25	0.20235923196332806
1107	26-27	0.1599712490722922
1107	28-29	0.24252105337728835
1107	30-31	0.2535480135752266
1107	32-33	0.2719759719056114
1107	34-35	0.22207427930248258
1107	36-37	0.2572806758792652
1107	38-39	0.2672917714893259
1107	40-41	0.25752146235014806
1107	42-43	0.20444672713151846
1107	44-45	0.25787925701166614
1107	46-47	0.23445086835162243
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2216	90-91	-1.0640844301713628
2216	92-93	-1.087083689385743
2216	94-95	-1.233218826441842
2216	96-97	-1.3096920902131899
2216	98-99	-1.310233879160144
2216	100	-1.308012780257478
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	522.0
3	70.0
4	94.0
5	132.0
6	428.0
7	1121.0
8	2510.0
9	4348.0
10	5979.0
11	8452.0
12	12086.0
13	16597.0
14	20579.0
15	26942.0
16	35048.0
17	50052.0
18	50612.0
19	27854.0
20	21035.0
21	20541.0
22	21958.0
23	23745.0
24	28026.0
25	35036.0
26	45156.0
27	59396.0
28	78259.0
29	103307.0
30	134616.0
31	175179.0
32	225036.0
33	291632.0
34	397135.0
35	572006.0
36	938305.0
37	1728205.0
38	2446534.0
39	972419.0
40	3124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.23352224227009	20.616120014272205	15.207765832337966	22.94259191111974
2	15.39320913255712	20.522484820116546	40.10141823366059	23.98288781366575
3	18.08667584024186	25.21817141413939	30.767772792319175	25.927379953299575
4	12.74532421963023	17.514867462988217	38.01434283877451	31.725465478607042
5	12.859918310116104	37.61901749630443	35.49092390930165	14.030140284277815
6	30.562932377005254	38.04539032407481	17.35732432550818	14.034352973411757
7	26.5985296495511	33.0540176950903	22.145773173490078	18.20167948186852
8	24.877132961078164	36.32477158869516	20.12157161702669	18.676523833199983
9	24.589396596534467	17.080700842047726	21.74353343179474	36.58636912962307
10-11	23.741320556807747	27.27145589111746	28.46338382838176	20.523839723693033
12-13	24.483293130977202	24.633513178008485	28.975944289389865	21.90724940162445
14-15	21.64569275082056	26.694524453712255	26.774725290727307	24.885057504739883
16-17	20.641050941301078	30.05046204157559	27.5551090181401	21.753377998983233
18-19	20.52020508672104	28.379484291611583	30.44819267676568	20.6521179449017
20-21	21.545021269615972	27.484950040050904	30.37805117289269	20.591977517440434
22-23	21.244825884579775	27.280332792952905	30.477986215406293	20.996855107061027
24-25	21.142147390120964	27.428817032840808	30.373170041830942	21.055865535207282
26-27	20.90760263539139	27.497397506732234	30.401297705192732	21.193702152683645
28-29	20.880297285691885	27.48037468491039	30.609002773277837	21.030325256119887
30-31	21.2078587302265	27.72565564412218	30.185739065035495	20.88074656061582
32-33	20.90937240289401	27.558924818215687	30.510710881569754	21.02099189732055
34-35	21.06455604540314	27.431572134263487	30.43278041806713	21.071091402266244
36-37	21.305641981734553	27.571633801937445	30.047019620981917	21.07570459534608
38-39	21.173204897067546	27.690796307022445	30.1167999910532	21.019198804856806
40-41	21.137260434320478	27.508703324621074	30.14964569279209	21.204390548266346
42-43	21.14297450302164	27.555050770752725	30.02490308799689	21.27707163822874
44-45	21.278312307579757	27.68798295821239	29.797237349715918	21.236467384491934
46-47	21.381142245245734	27.796864799426285	29.471745124344196	21.35024783098379
48-49	21.452553542163418	27.85918950391399	29.124468376095457	21.563788577827133
50-51	21.515041339335767	27.904948651433187	28.956313993492135	21.62369601573891
52-53	21.46012570252174	28.007446578991146	28.888461611942855	21.64396610654426
54-55	21.552698275271563	28.2025287287764	28.754679012627566	21.49009398332447
56-57	21.445959937912946	28.001953850361993	28.829975410282948	21.722110801442113
58-59	21.34470044799894	27.735760949028727	29.113611209986228	21.805927392986106
60-61	21.503298666041633	27.965199748930463	28.85093864499802	21.680562940029887
62-63	21.329109854106605	27.985662056114137	28.93865338564104	21.746574704138222
64-65	21.330239853421613	27.800429539533432	29.022081118573507	21.847249488471444
66-67	21.27287200159924	27.81778143623146	28.938001014902476	21.971345547266825
68-69	21.579108897632597	27.95684870199476	28.74679981554216	21.717242584830483
70-71	21.471885850032084	27.800982889713467	28.76913601417322	21.957995246081232
72-73	21.393816876737812	27.87145057895573	28.83807179712761	21.896660747178846
74-75	21.49735743252972	27.911495657773767	28.64417789404474	21.94696901565177
76-77	21.466375647186723	27.913027564061643	28.55263047531266	22.067966313438976
78-79	21.527307955339804	27.929572290608956	28.636585850444156	21.906533903607084
80-81	21.411954647750502	27.878604628432697	28.654287390317663	22.055153333499145
82-83	21.345780378261043	27.88024597493105	28.786601841753985	21.987371805053925
84-85	21.39813883288079	27.805671804396887	28.60321250650623	22.19297685621609
86-87	21.443910878929245	27.901775794026975	28.538180960780224	22.116132366263557
88-89	21.342071922638567	27.78777271225813	28.5755516569907	22.294603708112604
90-91	21.22888516907999	27.735487570993396	28.598131679448674	22.43749558047794
92-93	21.251090398713142	27.842311803705865	28.539986411954498	22.366611385626495
94-95	21.163114379068787	27.66351090062963	28.630213865352754	22.543160854948834
96-97	21.104863237464347	27.725529224112183	28.621205124465348	22.548402413958122
98-99	21.154765342745804	27.718817162901573	28.54700792968559	22.57940956466703
100	21.19032799094407	27.61560566203094	28.49593085357655	22.69813549344844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	609.0
1	494.5
2	641.0
3	930.5
4	656.0
5	465.0
6	1214.0
7	3499.0
8	6125.5
9	7321.0
10	6120.0
11	4414.0
12	4434.5
13	5017.5
14	5760.0
15	6579.0
16	7360.5
17	8331.0
18	9501.0
19	10928.0
20	13010.5
21	15835.0
22	20544.5
23	27837.5
24	36707.5
25	47136.5
26	61473.5
27	80614.5
28	104103.5
29	129412.0
30	152194.5
31	173659.0
32	196118.0
33	217461.5
34	237203.5
35	256236.5
36	271901.5
37	286655.5
38	298899.5
39	305786.5
40	310676.5
41	312559.0
42	310536.0
43	307710.5
44	303747.5
45	298151.5
46	292555.5
47	283785.0
48	273674.5
49	267998.0
50	261201.5
51	249315.5
52	235735.5
53	224994.5
54	215486.5
55	201993.5
56	184041.5
57	163611.0
58	146954.0
59	133981.5
60	116989.0
61	97271.0
62	80588.0
63	65813.5
64	52983.0
65	42106.0
66	33050.5
67	25467.0
68	19156.0
69	14330.0
70	10400.5
71	7661.5
72	5610.0
73	3836.0
74	2658.5
75	1819.0
76	1090.5
77	656.0
78	419.5
79	257.0
80	151.5
81	71.0
82	38.0
83	22.0
84	9.5
85	4.0
86	4.5
87	3.5
88	0.5
89	0.5
90	0.5
91	1.0
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06014625220000382
2	2.0969059453807256E-4
3	0.0
4	0.12022260753516162
5	0.0017357721436762675
6	0.0015377310266125324
7	0.0
8	0.0
9	2.3298954948674732E-5
10-11	0.0
12-13	2.3298954948674732E-5
14-15	1.1649477474337366E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.1649477474337368E-4
30-31	2.038658558009039E-4
32-33	2.3298954948674732E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.8056690085222916E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	5.824738737168683E-6
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.3396899095487972E-4
80-81	2.9706167559560284E-4
82-83	3.553090629672897E-4
84-85	0.0
86-87	5.824738737168683E-6
88-89	6.407212610885551E-5
90-91	3.49484324230121E-5
92-93	6.98968648460242E-4
94-95	6.232470448770491E-4
96-97	0.0
98-99	3.49484324230121E-5
100	0.0010368034952160255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8584076.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.627008328309316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26576311694124	40.19928206757719
2	15.71312063868103	17.48147785263277
3	5.412983730915636	9.033242732419406
4	2.3427393442031303	5.2127832404418175
5	1.2414329031028748	3.4528599219970415
6	0.722985099600418	2.4130498894029593
7	0.4935869830533064	1.921973705193594
8	0.3139593922251746	1.3971697380848571
9	0.23606642224734423	1.1818501952748477
>10	1.1682717255444337	11.355474422726536
>50	0.06101777573893175	2.2992638543160098
>100	0.02637351929489341	2.541816835445283
>500	0.0012379946161650507	0.45650444891735964
>1k	3.774713413127469E-4	0.33113214249196304
>5k	2.0970623509372236E-5	0.07801773029074845
>10k+	6.291187052811671E-5	0.6441012227876309
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22104	0.2575000500927531	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20989	0.24451088270886698	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12122	0.14121496594391755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1649477474337366E-5	0.0	0.0	0.0
3	0.0	1.1649477474337366E-5	0.0	0.0	0.0
4	0.0	1.1649477474337366E-5	0.0	1.1649477474337366E-5	0.0
5	0.0	2.3298954948674732E-5	0.0	1.1649477474337366E-5	0.0
6	0.0	2.3298954948674732E-5	0.0	1.1649477474337366E-5	0.0
7	0.0	2.3298954948674732E-5	0.0	1.1649477474337366E-5	0.0
8	0.0	2.3298954948674732E-5	0.0	1.1649477474337366E-5	0.0
9	2.3298954948674732E-5	2.3298954948674732E-5	0.0	2.3298954948674732E-5	0.0
10-11	2.3298954948674732E-5	2.3298954948674732E-5	0.0	3.4948432423012096E-5	0.0
12-13	2.3298954948674732E-5	2.3298954948674732E-5	0.0	5.8247387371686824E-5	0.0
14-15	2.3298954948674732E-5	4.077317116018078E-5	0.0	8.154634232036156E-5	0.0
16-17	2.3298954948674732E-5	7.572160358319288E-5	0.0	9.319581979469893E-5	0.0
18-19	2.9123693685843412E-5	1.2231951348054234E-4	0.0	9.319581979469893E-5	0.0
20-21	3.4948432423012096E-5	1.3979372969204838E-4	0.0	1.1649477474337365E-4	0.0
22-23	3.4948432423012096E-5	1.3979372969204838E-4	0.0	1.980411170637352E-4	0.0
24-25	3.4948432423012096E-5	1.4561846842921707E-4	0.0	3.902574953903018E-4	0.0
26-27	4.077317116018078E-5	1.5144320716638576E-4	0.0	8.853602880496398E-4	0.0
28-29	5.242264863451815E-5	1.747421621150605E-4	0.0	0.002900719891110004	0.0
30-31	5.824738737168683E-5	1.747421621150605E-4	0.0	0.008591489637323807	0.0
32-33	5.824738737168683E-5	1.747421621150605E-4	0.0	0.021603955976158645	0.0
34-35	6.989686484602419E-5	1.9804111706373523E-4	0.0	0.04247981961017121	0.0
36-37	6.989686484602419E-5	1.9804111706373523E-4	0.0	0.0749760370248353	0.0
38-39	6.989686484602419E-5	2.038658558009039E-4	0.0	0.12126523576911483	0.0
40-41	6.989686484602419E-5	2.2134007201240994E-4	0.0	0.16989597948573615	0.0
42-43	6.989686484602419E-5	2.2134007201240994E-4	0.0	0.21944703192283013	0.0
44-45	6.989686484602419E-5	2.2716481074957863E-4	0.0	0.28123003570797833	0.0
46-47	7.572160358319287E-5	2.3298954948674732E-4	0.0	0.3554838051294047	0.0
48-49	8.154634232036156E-5	2.3298954948674732E-4	0.0	0.43333143835166416	0.0
50-51	8.154634232036156E-5	2.6211324317259075E-4	0.0	0.5123556687988317	0.0
52-53	8.737108105753025E-5	2.6793798190975944E-4	0.0	0.5912983529036788	0.0
54-55	9.319581979469893E-5	2.6793798190975944E-4	0.0	0.6807954636002757	0.0
56-57	1.1067003600620498E-4	2.6793798190975944E-4	0.0	0.7880871511389228	0.0
58-59	1.2814425221771103E-4	2.7958745938409677E-4	0.0	0.906084708476486	0.0
60-61	1.2814425221771103E-4	2.7958745938409677E-4	0.0	1.03008757145207	0.0
62-63	1.2814425221771103E-4	2.7958745938409677E-4	0.0	1.1532691462657134	0.0
64-65	1.2814425221771103E-4	2.7958745938409677E-4	0.0	1.283300613834267	0.0
66-67	1.2814425221771103E-4	2.7958745938409677E-4	0.0	1.4342428934692564	0.0
68-69	1.2814425221771103E-4	2.7958745938409677E-4	0.0	1.6058513461437203	0.0
70-71	1.2814425221771103E-4	2.7958745938409677E-4	0.0	1.7857949999510723	0.0
72-73	1.2814425221771103E-4	3.0288641433277153E-4	0.0	1.9718954026036117	0.0
74-75	1.3979372969204838E-4	3.145358918071089E-4	0.0	2.160552865561768	0.0
76-77	1.3979372969204838E-4	3.145358918071089E-4	0.0	2.368554285866062	0.0
78-79	1.3979372969204838E-4	3.145358918071089E-4	0.0	2.603757236072933	0.0
80-81	1.3979372969204838E-4	3.378348467557836E-4	0.0	2.8555606916807355	0.0
82-83	1.3979372969204838E-4	3.49484324230121E-4	0.0	3.116258523340194	0.0
84-85	1.3979372969204838E-4	3.611338017044583E-4	0.0	3.379758054332231	0.0
86-87	1.3979372969204838E-4	3.611338017044583E-4	0.0	3.6629976249045324	0.0
88	1.3979372969204838E-4	3.611338017044583E-4	0.0	3.892300114770652	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15240	0.0	55.232975	1
GTATCAA	26740	0.0	41.003784	1
ATCAACG	33265	0.0	32.444084	3
TCAACGC	33505	0.0	32.26785	4
TATCAAC	34540	0.0	31.423525	2
GTGGTAT	6245	0.0	31.360048	1
CAACGCA	34820	0.0	31.11152	5
AACGCAG	36490	0.0	29.713087	6
ACGCAGA	41160	0.0	26.250504	7
TGGTATC	7280	0.0	25.785238	2
CGCAGAG	41660	0.0	25.732384	8
GTACATG	24200	0.0	23.247078	1
GCAGAGT	45340	0.0	23.156649	9
TACATGG	24725	0.0	21.749012	2
GAGTACT	30615	0.0	21.353394	12-13
CAGAGTA	43745	0.0	20.734947	10-11
AGAGTAC	41765	0.0	20.491394	10-11
ACATGGG	25670	0.0	20.141947	3
GTACTTT	33300	0.0	19.419956	14-15
AGTACTT	32690	0.0	19.171326	12-13
>>END_MODULE