FastQCFastQC Report
Wed 25 May 2016
SRR2049499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565488
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.042.5387951
GTATCAA51050.031.1303791
TACCTGG26600.030.0602342
TCAACGC51100.029.6906854
ATCAACG51650.029.4737553
CAACGCA54300.027.846565
GTACCTG28950.027.8015821
TATAACG3800.025.6282752
GTACATG76500.025.3307931
ATAACGC4250.025.3267673
TATCAAC61400.024.8769592
TCTGTCG13450.024.8735338
TACATGG77050.024.6803132
AACGCAG62550.024.2553126
GTCTCGC13750.023.8613871
ACATGGG81400.023.0465563
CGCTCTG13100.022.3024675
CTGTCGC15950.021.541759
ACGCAGA73000.020.8526977
CGCAGAG72400.020.6689078
Produced by FastQC (version 0.11.5)