FastQCFastQC Report
Wed 25 May 2016
SRR2049499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565488
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137390.3853329474114062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135950.38129422956969705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86680.24310837674955013No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61760.1732161207666384No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.13308136221465336No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40420.11336456608464256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73050.058.8920861
ATAACGC3800.042.094473
GTATCAA129900.039.4955641
TATAACG4600.034.7736932
GTGGTAT28050.031.7146471
ATCAACG163050.031.1048183
TCAACGC164250.030.791644
TATCAAC168450.029.9680482
CAACGCA170550.029.7069475
TGGTATC28850.028.700972
AACGCAG180350.028.1704926
TACCTGG29900.027.3783822
GTACCTG32450.025.6737631
TAACGCA6850.025.4121194
CGCAGAG199550.025.2708998
ACGCAGA201350.025.1616927
GCAGAGT212400.023.233129
GTACATG75000.022.4673981
GTATAAC10550.021.8612291
GTCTCGC13600.021.8038221
Produced by FastQC (version 0.11.5)