##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049499_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3565488 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.706962693465805 31.0 31.0 34.0 30.0 34.0 2 31.74481417410464 31.0 31.0 34.0 30.0 34.0 3 31.798174892188673 33.0 31.0 34.0 30.0 34.0 4 35.5347346562378 37.0 35.0 37.0 33.0 37.0 5 35.699420668362926 37.0 35.0 37.0 33.0 37.0 6 35.798439933047035 37.0 35.0 37.0 35.0 37.0 7 35.77440591582415 37.0 35.0 37.0 33.0 37.0 8 35.768765173238556 37.0 35.0 37.0 33.0 37.0 9 37.49255810144362 39.0 37.0 39.0 35.0 39.0 10-11 37.462558561408706 39.0 37.0 39.0 34.5 39.0 12-13 37.4651274944692 39.0 37.0 39.0 34.5 39.0 14-15 38.725736420933124 40.0 38.0 41.0 34.5 41.0 16-17 38.73092266752826 40.0 38.0 41.0 34.5 41.0 18-19 38.808684533505655 40.0 38.0 41.0 35.0 41.0 20-21 38.82736304259052 40.0 38.0 41.0 35.0 41.0 22-23 38.77957575512805 40.0 38.0 41.0 35.0 41.0 24-25 38.735424716055704 40.0 38.0 41.0 34.0 41.0 26-27 38.633064674456904 40.0 38.0 41.0 34.0 41.0 28-29 38.531285058314594 40.0 38.0 41.0 34.0 41.0 30-31 38.381138710886134 40.0 38.0 41.0 34.0 41.0 32-33 38.184740910641125 40.0 38.0 41.0 34.0 41.0 34-35 38.0471201978523 40.0 37.5 41.0 33.5 41.0 36-37 37.87701809682153 40.0 37.0 41.0 33.0 41.0 38-39 37.75903144254026 40.0 37.0 41.0 33.0 41.0 40-41 37.62225787886539 40.0 37.0 41.0 33.0 41.0 42-43 37.466997224503345 40.0 37.0 41.0 33.0 41.0 44-45 37.25909959590384 40.0 37.0 41.0 32.0 41.0 46-47 36.96241384068604 40.0 37.0 41.0 31.0 41.0 48-49 36.67996162096185 40.0 36.5 41.0 30.5 41.0 50-51 36.45910475648775 40.0 36.0 41.0 30.0 41.0 52-53 36.341670060311515 40.0 36.0 41.0 30.0 41.0 54-55 36.19752022724519 39.0 35.5 41.0 30.0 41.0 56-57 36.02466408525285 39.0 35.0 41.0 29.5 41.0 58-59 35.85512824611946 39.0 35.0 41.0 29.0 41.0 60-61 35.6430215162693 39.0 35.0 41.0 28.5 41.0 62-63 35.39996123952738 38.5 35.0 40.5 28.0 41.0 64-65 35.100508121188454 38.0 35.0 40.0 28.0 41.0 66-67 34.838476247851624 37.0 34.5 40.0 28.0 41.0 68-69 34.504776204547596 37.0 34.0 40.0 28.0 41.0 70-71 34.18746045422114 36.0 34.0 39.0 27.0 41.0 72-73 33.86362582064503 36.0 34.0 39.0 26.0 41.0 74-75 33.526970782120145 35.0 34.0 39.0 26.0 40.5 76-77 33.25165657548139 35.0 34.0 37.5 26.0 40.0 78-79 32.93457080769869 35.0 33.5 37.0 26.0 39.0 80-81 32.634994284092386 35.0 33.0 36.5 26.0 39.0 82-83 32.36790013036084 35.0 33.0 36.0 26.0 38.5 84-85 32.102039748836624 35.0 33.0 36.0 25.0 37.0 86-87 31.84606020269876 35.0 33.0 35.5 25.0 37.0 88-89 31.641986594822363 35.0 33.0 35.0 24.5 36.5 90-91 31.50062978756344 35.0 33.0 35.0 24.0 36.0 92-93 31.340298018111405 35.0 33.0 35.0 24.0 36.0 94-95 31.15090781402153 35.0 33.0 35.0 23.5 36.0 96-97 30.97706457012336 35.0 33.0 35.0 21.0 35.5 98-99 30.801722933859264 35.0 33.0 35.0 19.5 35.0 100 30.693850042406538 35.0 33.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40633798734602067 1101 2 0.46461749203362857 1101 3 0.5393317538312203 1101 4 0.41913279727059205 1101 5 0.3435149548948502 1101 6 0.32350760071730633 1101 7 0.3503042814733064 1101 8 0.3772350751145481 1101 9 0.3671162806582302 1101 10-11 0.44820072572259306 1101 12-13 0.37238074957154055 1101 14-15 0.5395241983671681 1101 16-17 0.5732087251703604 1101 18-19 0.501851536063775 1101 20-21 0.5015934956029824 1101 22-23 0.547667691587975 1101 24-25 0.5731282482820959 1101 26-27 0.5906519435816691 1101 28-29 0.5912879715796464 1101 30-31 0.5997730940178059 1101 32-33 0.6231035656328245 1101 34-35 0.67501274859994 1101 36-37 0.6287431044548484 1101 38-39 0.6881713762197634 1101 40-41 0.7647858958255327 1101 42-43 0.7156899836538315 1101 44-45 0.7146846185226607 1101 46-47 0.7592343704271585 1101 48-49 0.7362457651030141 1101 50-51 0.7812899403543909 1101 52-53 0.7882811820917652 1101 54-55 0.6800180625870667 1101 56-57 0.7324959391478671 1101 58-59 0.7138225334558115 1101 60-61 0.7427799782707183 1101 62-63 0.7506649587313703 1101 64-65 0.8088840815056955 1101 66-67 0.7188257013730848 1101 68-69 0.6793953261276542 1101 70-71 0.6252180060835997 1101 72-73 0.6316033431415562 1101 74-75 0.6575976440296785 1101 76-77 0.6146488312908076 1101 78-79 0.5391915053993728 1101 80-81 0.5443232264036766 1101 82-83 0.649920980071947 1101 84-85 0.6597332907403555 1101 86-87 0.6927679828172373 1101 88-89 0.6868779591077256 1101 90-91 0.7002940509663667 1101 92-93 0.759918320018869 1101 94-95 0.6971526907069645 1101 96-97 0.7466108106170957 1101 98-99 0.8207136789861167 1101 100 0.8828097013498137 1102 1 0.28293461544584275 1102 2 0.3430529912251892 1102 3 0.4400297660667718 1102 4 0.39640624213081566 1102 5 0.33052817728960804 1102 6 0.2974036505409856 1102 7 0.2884182049585746 1102 8 0.32687550362419415 1102 9 0.43644073188637833 1102 10-11 0.3343768527130493 1102 12-13 0.3521514414293918 1102 14-15 0.45264002958904115 1102 16-17 0.45510224419533074 1102 18-19 0.46577638066325733 1102 20-21 0.43779396466525355 1102 22-23 0.46777628549367023 1102 24-25 0.4379244736031538 1102 26-27 0.3894310424985079 1102 28-29 0.4474933946437005 1102 30-31 0.4724687693569223 1102 32-33 0.48201932322074725 1102 34-35 0.5129499124670005 1102 36-37 0.5954367023928668 1102 38-39 0.5668731134503915 1102 40-41 0.6090801559081811 1102 42-43 0.6512966090126895 1102 44-45 0.6514197213293471 1102 46-47 0.64531912025042 1102 48-49 0.6468127631224903 1102 50-51 0.5965822167505834 1102 52-53 0.6157115899820269 1102 54-55 0.5961270264606142 1102 56-57 0.5951016477143156 1102 58-59 0.6067334381645679 1102 60-61 0.6275353719149948 1102 62-63 0.5816349462406478 1102 64-65 0.6061529938282746 1102 66-67 0.6329639787254067 1102 68-69 0.5700354309154321 1102 70-71 0.6520796387565895 1102 72-73 0.6556557122914981 1102 74-75 0.5981909418435123 1102 76-77 0.5332063148650263 1102 78-79 0.47987945284092604 1102 80-81 0.5005430986396249 1102 82-83 0.5113482432355241 1102 84-85 0.49375202474980284 1102 86-87 0.5405607009955986 1102 88-89 0.5035257893477123 1102 90-91 0.5525378728665551 1102 92-93 0.5912707538772288 1102 94-95 0.5573319417105154 1102 96-97 0.5914514457448661 1102 98-99 0.64002752168836 1102 100 0.6408914741689848 1103 1 0.25086057960916364 1103 2 0.3141433787533039 1103 3 0.3490062232986375 1103 4 0.27773274828502537 1103 5 0.1882023314713095 1103 6 0.25872456280640677 1103 7 0.2554467009715111 1103 8 0.25241376859418807 1103 9 0.3551492151748832 1103 10-11 0.25139504328487305 1103 12-13 0.23111280911862053 1103 14-15 0.33705880720135895 1103 16-17 0.3881946427872691 1103 18-19 0.39807552284842274 1103 20-21 0.37449243851337144 1103 22-23 0.30947272209116505 1103 24-25 0.37537930740779046 1103 26-27 0.393517289816522 1103 28-29 0.36785966169786377 1103 30-31 0.3357096927437979 1103 32-33 0.3990539064570342 1103 34-35 0.4633050304364872 1103 36-37 0.47673275515673197 1103 38-39 0.4503755255731008 1103 40-41 0.4651809425004245 1103 42-43 0.4102641737854782 1103 44-45 0.47337531334221694 1103 46-47 0.5053117395784881 1103 48-49 0.6185883951175768 1103 50-51 0.6289202453069151 1103 52-53 0.6447607560220661 1103 54-55 0.6484986356232909 1103 56-57 0.6860723332082159 1103 58-59 0.585746561071133 1103 60-61 0.5402017678234401 1103 62-63 0.6459960534575089 1103 64-65 0.6435619178082561 1103 66-67 0.604590522114627 1103 68-69 0.692835436885197 1103 70-71 0.7201702585112386 1103 72-73 0.7384374932112934 1103 74-75 0.6594009782030952 1103 76-77 0.6561508146579911 1103 78-79 0.6019342921593065 1103 80-81 0.593755727147574 1103 82-83 0.677088168725426 1103 84-85 0.6577591816938266 1103 86-87 0.7385792165187439 1103 88-89 0.704574529489399 1103 90-91 0.6468841171706607 1103 92-93 0.6791699308031021 1103 94-95 0.6659224268345767 1103 96-97 0.6532790485320561 1103 98-99 0.7284908204645539 1103 100 0.7431322633052879 1104 1 0.25567348395451006 1104 2 0.2653210852831549 1104 3 0.3314234464474737 1104 4 0.3111547506353318 1104 5 0.22577327479238818 1104 6 0.22689722144146884 1104 7 0.16224471833888998 1104 8 0.1704128640287621 1104 9 0.23385842070032936 1104 10-11 0.2706442699199485 1104 12-13 0.22954185636736923 1104 14-15 0.3240322615166349 1104 16-17 0.28766572005324065 1104 18-19 0.26138586506084494 1104 20-21 0.2948504700379502 1104 22-23 0.31584575441979723 1104 24-25 0.2759751976020155 1104 26-27 0.266722798135838 1104 28-29 0.27501773541911234 1104 30-31 0.34913730272236165 1104 32-33 0.3349094527842382 1104 34-35 0.2818468219601584 1104 36-37 0.3491468766442978 1104 38-39 0.35511353314970506 1104 40-41 0.3350626825745735 1104 42-43 0.261859410874294 1104 44-45 0.23477330423207832 1104 46-47 0.33928197990061904 1104 48-49 0.3090980686913838 1104 50-51 0.30544672602642464 1104 52-53 0.3130398261107459 1104 54-55 0.24651938325016687 1104 56-57 0.24829592184525495 1104 58-59 0.30379862813860115 1104 60-61 0.2119897893809366 1104 62-63 0.2775514673539661 1104 64-65 0.2714088857392767 1104 66-67 0.30785080940463416 1104 68-69 0.23459511714147396 1104 70-71 0.2411383155175173 1104 72-73 0.3028380223764202 1104 74-75 0.3127085474818614 1104 76-77 0.23518742624794697 1104 78-79 0.23657790277858481 1104 80-81 0.1755499483750853 1104 82-83 0.21624080655369227 1104 84-85 0.21555004445406212 1104 86-87 0.19663903491698775 1104 88-89 0.1810877382464824 1104 90-91 0.20270699727018737 1104 92-93 0.25775703228188007 1104 94-95 0.24055257755220438 1104 96-97 0.2113082222114393 1104 98-99 0.31222883464891993 1104 100 0.3246460699149658 1105 1 0.24664782402717123 1105 2 0.2558649374173072 1105 3 0.35584349736601695 1105 4 0.3211968601827735 1105 5 0.24252032292592673 1105 6 0.2793747684713921 1105 7 0.2309804784382763 1105 8 0.26777785091488937 1105 9 0.2898311028607736 1105 10-11 0.2556568857069763 1105 12-13 0.30566671067743556 1105 14-15 0.33069397342129747 1105 16-17 0.3760498746816552 1105 18-19 0.39228123727993136 1105 20-21 0.3491095611605104 1105 22-23 0.3448421411673195 1105 24-25 0.2865472342440043 1105 26-27 0.34808199442552734 1105 28-29 0.3611325782162993 1105 30-31 0.34620891741502646 1105 32-33 0.35440041680683265 1105 34-35 0.33624466539347253 1105 36-37 0.30788336252500415 1105 38-39 0.286860915711884 1105 40-41 0.30383932123936574 1105 42-43 0.27541088655848966 1105 44-45 0.416160820224178 1105 46-47 0.4146050948971123 1105 48-49 0.5645202599532624 1105 50-51 0.530797378019976 1105 52-53 0.46174014388911644 1105 54-55 0.43805378457307853 1105 56-57 0.4491480744984102 1105 58-59 0.3983905255728999 1105 60-61 0.4398885030402795 1105 62-63 0.47427378199866865 1105 64-65 0.496853106598806 1105 66-67 0.5816498547887434 1105 68-69 0.576446478550686 1105 70-71 0.534583798667299 1105 72-73 0.5286577639128893 1105 74-75 0.5463439576408078 1105 76-77 0.4127918071209109 1105 78-79 0.4058644383966552 1105 80-81 0.4107738946046453 1105 82-83 0.418228059841411 1105 84-85 0.435690547178595 1105 86-87 0.46858502540213465 1105 88-89 0.4244144636492493 1105 90-91 0.4057192387817459 1105 92-93 0.3708341173733345 1105 94-95 0.4403142793528545 1105 96-97 0.46536334743717944 1105 98-99 0.5342387579295611 1105 100 0.5156503662557448 1106 1 0.17140075528074306 1106 2 0.24319520770857395 1106 3 0.28720111307631413 1106 4 0.24468184739235 1106 5 0.140486895814675 1106 6 0.1918436247692128 1106 7 0.20270421645939507 1106 8 0.24134246612022991 1106 9 0.22047151171030066 1106 10-11 0.21980764064696956 1106 12-13 0.21055009866831398 1106 14-15 0.2923879133701419 1106 16-17 0.30637884650943903 1106 18-19 0.2983930327663771 1106 20-21 0.2701710273144542 1106 22-23 0.29160028292179163 1106 24-25 0.282229426982596 1106 26-27 0.2798044561791144 1106 28-29 0.30522304338806805 1106 30-31 0.25675782343178355 1106 32-33 0.3530173312358684 1106 34-35 0.33627518483435637 1106 36-37 0.3843636941326025 1106 38-39 0.35780404967002966 1106 40-41 0.39948516809835155 1106 42-43 0.3699261268574645 1106 44-45 0.3831776281126196 1106 46-47 0.4428328032200426 1106 48-49 0.44522878264584875 1106 50-51 0.48023082621738666 1106 52-53 0.4212387649652882 1106 54-55 0.41471265490984877 1106 56-57 0.4640360126604719 1106 58-59 0.4224044701828902 1106 60-61 0.3857379978048101 1106 62-63 0.4337599178490237 1106 64-65 0.38968478355884884 1106 66-67 0.4172714525647052 1106 68-69 0.4651254306795565 1106 70-71 0.5263234345426184 1106 72-73 0.4920892311211418 1106 74-75 0.46882943316144576 1106 76-77 0.5340032347093739 1106 78-79 0.5055402386996306 1106 80-81 0.46813657099313843 1106 82-83 0.461560116907215 1106 84-85 0.4515044729220108 1106 86-87 0.5657984617247109 1106 88-89 0.5307962174628003 1106 90-91 0.4494695509123936 1106 92-93 0.4804093145011805 1106 94-95 0.44962548331704966 1106 96-97 0.43220605578785154 1106 98-99 0.47493809704494083 1106 100 0.5451293716549124 1107 1 0.18165516666150694 1107 2 0.16271143299178803 1107 3 0.24871231893551737 1107 4 0.2749377653855234 1107 5 0.2150804049161792 1107 6 0.18704724809844464 1107 7 0.24976413841000067 1107 8 0.22849700038560172 1107 9 0.1827651009027349 1107 10-11 0.16617432458814818 1107 12-13 0.10614314695254734 1107 14-15 0.21064314823481567 1107 16-17 0.14962263455508662 1107 18-19 0.22139170429739607 1107 20-21 0.2540684937518378 1107 22-23 0.26542068392607376 1107 24-25 0.3177948041460823 1107 26-27 0.23703181576971133 1107 28-29 0.2980718486984131 1107 30-31 0.26861607770477747 1107 32-33 0.32684466412948154 1107 34-35 0.23967556487365727 1107 36-37 0.2935363136359115 1107 38-39 0.32572958424752585 1107 40-41 0.33959241730873657 1107 42-43 0.2459870739915644 1107 44-45 0.25099803107990226 1107 46-47 0.18487603710714495 1107 48-49 0.2021869272845649 1107 50-51 0.3046988865250668 1107 52-53 0.2526124593163246 1107 54-55 0.21970431455874717 1107 56-57 0.2944621852171636 1107 58-59 0.24757166980755585 1107 60-61 0.22634177886212115 1107 62-63 0.19859630808421258 1107 64-65 0.2262647562140785 1107 66-67 0.2702501283554497 1107 68-69 0.22492969866222978 1107 70-71 0.23404056585444266 1107 72-73 0.2610151906909266 1107 74-75 0.27825057009926013 1107 76-77 0.22265147457384415 1107 78-79 0.1658442897693746 1107 80-81 0.18013822335926477 1107 82-83 0.19732686238884867 1107 84-85 0.1458555720129482 1107 86-87 0.1745315871749753 1107 88-89 0.14930201865670512 1107 90-91 0.162455470220781 1107 92-93 0.14932633009339114 1107 94-95 0.15886775297220623 1107 96-97 0.18867675978317067 1107 98-99 0.14041254008910542 1107 100 0.10391314283827668 1108 1 0.09621177976601203 1108 2 0.11489910365219913 1108 3 0.19242794283744402 1108 4 0.1661072142738007 1108 5 0.17048625194372136 1108 6 0.17422738258788684 1108 7 0.12371170743453064 1108 8 0.1678568524288977 1108 9 0.1296118086299245 1108 10-11 0.0989792201831321 1108 12-13 0.15517975763255976 1108 14-15 0.16666313174055603 1108 16-17 0.18401646142607575 1108 18-19 0.19309176457230137 1108 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-0.6966560104257127 2215 48-49 -0.7189606515447267 2215 50-51 -0.6802263732707203 2215 52-53 -0.833691058936239 2215 54-55 -0.7272796150001497 2215 56-57 -0.6835707702945655 2215 58-59 -0.6874693229487221 2215 60-61 -0.8832422835202678 2215 62-63 -0.8018936993641077 2215 64-65 -0.6999679551418652 2215 66-67 -0.5319495798940821 2215 68-69 -0.4723316569617779 2215 70-71 -0.6059980919412951 2215 72-73 -0.5316605986569982 2215 74-75 -0.7361156626053571 2215 76-77 -0.6499430928616832 2215 78-79 -0.6359807943922391 2215 80-81 -0.6771135158091468 2215 82-83 -0.7904753300820673 2215 84-85 -0.6677529209138484 2215 86-87 -0.5283299017631862 2215 88-89 -0.5890094552670568 2215 90-91 -0.6354204823474419 2215 92-93 -0.7513676199361221 2215 94-95 -0.7687236147025658 2215 96-97 -0.916283028847392 2215 98-99 -0.9170370420590643 2215 100 -0.9692613743333425 2216 1 -0.8123243309225074 2216 2 -1.2847856252352834 2216 3 -0.9129696170068797 2216 4 -1.2907600835605209 2216 5 -0.7517626242993671 2216 6 -1.0043645471011473 2216 7 -0.7812172841937866 2216 8 -0.7554862865224763 2216 9 -0.7820389129145084 2216 10-11 -1.0162293504552053 2216 12-13 -0.8526729759047669 2216 14-15 -1.1001295810832445 2216 16-17 -1.1258071932964953 2216 18-19 -1.1964911661394595 2216 20-21 -0.9465891019089483 2216 22-23 -1.0157813331223409 2216 24-25 -1.0610748831722674 2216 26-27 -1.2523807103271523 2216 28-29 -1.428652336126909 2216 30-31 -1.5004476394942259 2216 32-33 -1.2699682315613146 2216 34-35 -1.3823278795741416 2216 36-37 -1.3266657764559966 2216 38-39 -1.5569781805844727 2216 40-41 -1.3429561504531335 2216 42-43 -1.8935784581996558 2216 44-45 -1.6573840415801868 2216 46-47 -1.6110861406924428 2216 48-49 -1.5665759285251042 2216 50-51 -1.7609158060974153 2216 52-53 -1.676402725563861 2216 54-55 -1.7517479481253986 2216 56-57 -1.8426670612451517 2216 58-59 -1.6533143139157644 2216 60-61 -1.868361793211463 2216 62-63 -1.9689703872553608 2216 64-65 -1.878162654421672 2216 66-67 -1.6846170033650836 2216 68-69 -1.9124853844193623 2216 70-71 -1.743531208627914 2216 72-73 -1.5719192478388067 2216 74-75 -1.686488529006283 2216 76-77 -1.7362689548602788 2216 78-79 -1.722265708190335 2216 80-81 -1.556083966176594 2216 82-83 -1.7436503124427674 2216 84-85 -1.7479803465568082 2216 86-87 -1.8258159473141902 2216 88-89 -1.6736113220138549 2216 90-91 -1.538299497507552 2216 92-93 -1.6470770943830217 2216 94-95 -1.8113883068642878 2216 96-97 -1.9061384386270035 2216 98-99 -1.9068953948078757 2216 100 -1.979526840679167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 179.0 3 20.0 4 33.0 5 118.0 6 297.0 7 792.0 8 1661.0 9 2689.0 10 3982.0 11 6310.0 12 9448.0 13 13611.0 14 13890.0 15 15881.0 16 20227.0 17 28811.0 18 28991.0 19 15257.0 20 11497.0 21 10699.0 22 11314.0 23 12091.0 24 13818.0 25 16600.0 26 20829.0 27 27067.0 28 34614.0 29 45119.0 30 57811.0 31 74578.0 32 95349.0 33 123882.0 34 168897.0 35 245570.0 36 389052.0 37 730894.0 38 952586.0 39 359619.0 40 1405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4542423195577 20.19914300202982 13.513924603373942 22.83269007503854 2 17.407595839884582 20.893019112724858 38.465024885863826 23.23436016152673 3 18.055508811136093 25.240359804885053 30.848456087918404 25.855675296060454 4 13.19626470532903 18.109221175280698 37.36566650165568 31.32884761773459 5 11.863088565218941 35.99120891202713 34.3558937788412 17.789808743912726 6 29.073438341410878 37.87580214503679 18.030846122778676 15.019913390773649 7 25.47415669327733 32.42765085733005 22.98577361640258 19.11241883299004 8 24.488176653518394 34.001881369394596 21.931948726233266 19.57799325085374 9 23.839667299212504 16.71836602359398 23.423510848170487 36.018455829023026 10-11 23.045372751219467 26.293217085571456 29.012213755872967 21.649196407336106 12-13 24.712735635730038 23.986523581978624 29.158887809871718 22.14185297241962 14-15 22.232706995508046 26.29677901033463 27.52173054572053 23.948783448436792 16-17 20.343863168239523 29.21398136804836 27.794750676485236 22.647404787226883 18-19 19.81341684504337 26.672954725972996 31.72588156235556 21.787746866628073 20-21 21.615147772198366 26.745343133955295 31.046058772319522 20.593450321526817 22-23 20.966891488626523 27.146494392913397 31.09289668062268 20.7937174378374 24-25 20.67745004330403 26.628164223242372 31.577318448414353 21.117067285039244 26-27 20.529633044569643 26.19162176280242 31.783956611823772 21.494788580804165 28-29 20.837979151489314 26.313297008188936 31.606297324415415 21.24242651590633 30-31 21.466590753559128 27.376706642583887 30.726508071844467 20.430194532012518 32-33 20.316228891032274 26.802397540644517 31.70618768207541 21.175185886247796 34-35 20.277925490143282 25.858522031205826 31.905183806536442 21.958368672114446 36-37 21.77097496892431 27.04625565981431 30.464693753000994 20.71807561826039 38-39 21.422831881638643 26.944881037322236 30.839382996100394 20.792904084938723 40-41 20.677786603124172 26.734390916474826 31.125991729603353 21.46183075079765 42-43 20.685611618942485 26.831670727821834 30.785435261596728 21.697282391638957 44-45 21.458689525809653 27.13493917242184 30.34552353001889 21.060847771749618 46-47 21.07663803664463 28.2069943861822 29.45856219401103 21.257805383162136 48-49 21.036349021508414 27.277402139622964 30.05874651660586 21.62750232226276 50-51 21.257048123566815 26.816637722522135 29.911571711922747 22.014742441988304 52-53 21.193410270908217 27.22087411316487 29.53984139057543 22.04587422535148 54-55 21.609187858716673 28.14707271487101 29.062305075770833 21.181434350641485 56-57 20.7921468253434 27.708745619113007 29.195624834524754 22.303482721018835 58-59 20.445465343424527 26.873693200223812 30.040401235736514 22.640440220615147 60-61 21.558213069290936 27.687724653679947 29.047215977167784 21.706846299861336 62-63 21.27574121691056 27.54512145322043 29.246164900849475 21.932972429019536 64-65 21.102062326391227 26.99300909160261 29.876246954133627 22.028681627872537 66-67 21.00385697553883 27.200778687237204 29.201963377804102 22.59340095941986 68-69 21.913875732280648 27.638745052394658 28.847499815943824 21.59987939938087 70-71 22.010100721135508 26.97766757313445 29.14971246572699 21.86251924000305 72-73 21.341580731725923 27.215699505930186 29.781687667999446 21.66103209434445 74-75 21.276961246258576 27.105756631350324 29.50677438824643 22.110507734144665 76-77 21.406550800339254 27.493291240918495 28.83724471937642 22.26291323936583 78-79 21.698165199754815 27.71934016803051 28.91174506907689 21.67074956313779 80-81 21.254952004992322 27.170166667835282 29.260813776565538 22.31406755060686 82-83 20.94049995007679 27.301417819107463 29.408625945035112 22.349456285780636 84-85 21.26965509349632 27.271652576028867 29.086635546101963 22.372056784372855 86-87 21.56291088344709 27.40413093523243 28.962066342671744 22.070891838648734 88-89 20.910761980060506 27.279260869553024 29.182394999617163 22.627582150769307 90-91 20.658274824487485 26.996890887120173 29.120892758954493 23.223941529437848 92-93 21.093419134372077 27.495850462969972 28.60454393236437 22.806186470293575 94-95 20.989485505232285 27.110745068166537 29.018676136789118 22.881093289812053 96-97 20.704433825669 27.08339322462917 29.223058557911084 22.989114391790743 98-99 20.707992846981956 26.97935037223516 29.169176841991895 23.143479938790986 100 21.029106052429935 27.22322942107631 28.700156277871702 23.04750824862205 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 726.0 1 670.0 2 2680.0 3 4474.5 4 2675.5 5 1120.0 6 1667.0 7 3775.0 8 5930.0 9 6662.5 10 5333.0 11 3495.0 12 3217.0 13 3558.0 14 4013.5 15 4373.5 16 4797.0 17 5370.5 18 6014.0 19 6721.5 20 7725.5 21 9089.5 22 11068.5 23 14311.5 24 18225.5 25 22604.5 26 28500.5 27 36103.5 28 45780.0 29 55040.0 30 62417.0 31 70417.5 32 78427.0 33 85300.5 34 91482.5 35 98011.0 36 102982.0 37 106621.0 38 109977.5 39 111647.5 40 113299.5 41 113173.5 42 110754.5 43 110244.5 44 110138.5 45 109345.0 46 110012.0 47 111491.5 48 113774.5 49 116810.0 50 117057.0 51 112934.0 52 106356.5 53 101247.5 54 97929.0 55 93977.5 56 89111.0 57 82573.5 58 75877.5 59 68934.0 60 59580.0 61 48863.0 62 38799.5 63 29709.5 64 22413.0 65 16833.5 66 12359.0 67 8939.5 68 6339.5 69 4472.5 70 3100.0 71 2138.5 72 1499.0 73 1043.0 74 664.5 75 412.5 76 256.5 77 146.0 78 100.5 79 59.5 80 29.5 81 16.0 82 7.0 83 5.0 84 4.5 85 2.5 86 1.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05943085490681781 2 2.804665167853601E-4 3 0.0 4 0.11524369174710446 5 0.0016547524490336245 6 0.0013462392805697284 7 0.0 8 0.0 9 5.609330335707202E-5 10-11 0.0 12-13 1.4023325839268005E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.4023325839268005E-5 28-29 1.5425658423194805E-4 30-31 2.2437321342828808E-4 32-33 2.804665167853601E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.413995503560803E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.4023325839268005E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.2620993255341205E-4 80-81 3.646064718209681E-4 82-83 3.365598201424321E-4 84-85 0.0 86-87 0.0 88-89 2.804665167853601E-5 90-91 4.206997751780401E-5 92-93 8.413995503560803E-4 94-95 8.133528986775443E-4 96-97 1.4023325839268005E-5 98-99 0.0 100 0.0012340526738555844 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3565488.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.22749050871869 #Duplication Level Percentage of deduplicated Percentage of total 1 82.91945669818905 58.23225358260182 2 11.4257652409181 16.048056400228475 3 2.6830861571405804 5.652792229039939 4 1.015707605522772 2.8532238490593547 5 0.5509464056328408 1.93457917361965 6 0.32971153692134764 1.3892888297855395 7 0.210356703367185 1.0340976372415038 8 0.15533543814549713 0.8727054406424448 9 0.11174508913297092 0.7062819467833474 >10 0.5616271000413614 6.785682354967168 >50 0.023736018069669525 1.1380562912485654 >100 0.011528293860846245 1.4401843682856261 >500 5.587193174976709E-4 0.28570472847683576 >1k 2.793596529000615E-4 0.44498076356300487 >5k 7.981704368573175E-5 0.4160291273117869 >10k+ 7.981704368573175E-5 0.7660832771450001 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13739 0.3853329474114062 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13595 0.38129422956969705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8668 0.24310837674955013 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6176 0.1732161207666384 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4745 0.13308136221465336 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4042 0.11336456608464256 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.804665167853601E-5 0.0 4 0.0 0.0 0.0 2.804665167853601E-5 0.0 5 0.0 0.0 0.0 2.804665167853601E-5 0.0 6 0.0 0.0 0.0 2.804665167853601E-5 0.0 7 0.0 0.0 0.0 2.804665167853601E-5 0.0 8 0.0 0.0 0.0 2.804665167853601E-5 0.0 9 0.0 0.0 0.0 5.609330335707202E-5 0.0 10-11 0.0 0.0 0.0 5.609330335707202E-5 0.0 12-13 0.0 0.0 0.0 8.413995503560803E-5 0.0 14-15 0.0 7.011662919634003E-5 0.0 1.2620993255341205E-4 0.0 16-17 0.0 1.4023325839268005E-4 0.0 1.6827991007121605E-4 0.0 18-19 0.0 1.4023325839268005E-4 0.0 1.8230323591048405E-4 0.0 20-21 0.0 1.4023325839268005E-4 0.0 2.664431909460921E-4 0.0 22-23 0.0 1.4023325839268005E-4 0.0 4.6276975269584416E-4 0.0 24-25 0.0 1.4023325839268005E-4 0.0 9.255395053916883E-4 0.0 26-27 0.0 1.4023325839268005E-4 2.804665167853601E-5 0.0014163559097660687 0.0 28-29 2.804665167853601E-5 1.4023325839268005E-4 2.804665167853601E-5 0.0034918081339777333 0.0 30-31 2.804665167853601E-5 1.4023325839268005E-4 2.804665167853601E-5 0.009101138469684935 0.0 32-33 2.804665167853601E-5 1.4023325839268005E-4 2.804665167853601E-5 0.02346102412909537 0.0 34-35 2.804665167853601E-5 1.4023325839268005E-4 2.804665167853601E-5 0.04669767504476245 0.0 36-37 2.804665167853601E-5 1.5425658423194805E-4 2.804665167853601E-5 0.0802554937781308 0.0 38-39 2.804665167853601E-5 1.6827991007121605E-4 2.804665167853601E-5 0.12625200253092983 0.0 40-41 2.804665167853601E-5 1.6827991007121605E-4 2.804665167853601E-5 0.18801072952706616 0.0 42-43 2.804665167853601E-5 1.6827991007121605E-4 2.804665167853601E-5 0.25279849490448436 0.0 44-45 2.804665167853601E-5 1.9632656174975205E-4 2.804665167853601E-5 0.3246960864824114 0.0 46-47 2.804665167853601E-5 2.2437321342828808E-4 5.609330335707202E-5 0.41127610021405203 1.4023325839268005E-5 48-49 2.804665167853601E-5 2.2437321342828808E-4 5.609330335707202E-5 0.5103088272909627 2.804665167853601E-5 50-51 2.804665167853601E-5 2.2437321342828808E-4 5.609330335707202E-5 0.6168019637143639 2.804665167853601E-5 52-53 5.609330335707202E-5 2.2437321342828808E-4 5.609330335707202E-5 0.7281611942039912 2.804665167853601E-5 54-55 5.609330335707202E-5 2.2437321342828808E-4 5.609330335707202E-5 0.856404509004097 2.804665167853601E-5 56-57 5.609330335707202E-5 2.2437321342828808E-4 5.609330335707202E-5 1.004532899844285 2.804665167853601E-5 58-59 5.609330335707202E-5 2.2437321342828808E-4 5.609330335707202E-5 1.168002809152632 4.206997751780401E-5 60-61 7.011662919634003E-5 2.2437321342828808E-4 5.609330335707202E-5 1.3345157801681005 5.609330335707202E-5 62-63 8.413995503560803E-5 2.2437321342828808E-4 5.609330335707202E-5 1.5078440875414532 5.609330335707202E-5 64-65 8.413995503560803E-5 2.383965392675561E-4 5.609330335707202E-5 1.7131596011541759 5.609330335707202E-5 66-67 8.413995503560803E-5 2.524198651068241E-4 5.609330335707202E-5 1.9544589688704603 5.609330335707202E-5 68-69 8.413995503560803E-5 2.524198651068241E-4 5.609330335707202E-5 2.207594023595087 5.609330335707202E-5 70-71 8.413995503560803E-5 2.524198651068241E-4 5.609330335707202E-5 2.479520334944333 5.609330335707202E-5 72-73 8.413995503560803E-5 2.524198651068241E-4 5.609330335707202E-5 2.800752659944445 5.609330335707202E-5 74-75 1.1218660671414404E-4 2.524198651068241E-4 5.609330335707202E-5 3.1273418954151575 5.609330335707202E-5 76-77 1.1218660671414404E-4 2.524198651068241E-4 5.609330335707202E-5 3.4678562934442634 5.609330335707202E-5 78-79 1.1218660671414404E-4 2.524198651068241E-4 5.609330335707202E-5 3.849178569665639 5.609330335707202E-5 80-81 1.1218660671414404E-4 2.804665167853601E-4 5.609330335707202E-5 4.2686442921698236 5.609330335707202E-5 82-83 1.1218660671414404E-4 2.804665167853601E-4 5.609330335707202E-5 4.714501913903511 5.609330335707202E-5 84-85 1.1218660671414404E-4 2.804665167853601E-4 5.609330335707202E-5 5.161775891546964 5.609330335707202E-5 86-87 1.1218660671414404E-4 2.804665167853601E-4 5.609330335707202E-5 5.63945243960995 5.609330335707202E-5 88 1.1218660671414404E-4 2.804665167853601E-4 5.609330335707202E-5 6.023943987471 5.609330335707202E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7305 0.0 58.892086 1 ATAACGC 380 0.0 42.09447 3 GTATCAA 12990 0.0 39.495564 1 TATAACG 460 0.0 34.773693 2 GTGGTAT 2805 0.0 31.714647 1 ATCAACG 16305 0.0 31.104818 3 TCAACGC 16425 0.0 30.79164 4 TATCAAC 16845 0.0 29.968048 2 CAACGCA 17055 0.0 29.706947 5 TGGTATC 2885 0.0 28.70097 2 AACGCAG 18035 0.0 28.170492 6 TACCTGG 2990 0.0 27.378382 2 GTACCTG 3245 0.0 25.673763 1 TAACGCA 685 0.0 25.412119 4 CGCAGAG 19955 0.0 25.270899 8 ACGCAGA 20135 0.0 25.161692 7 GCAGAGT 21240 0.0 23.23312 9 GTACATG 7500 0.0 22.467398 1 GTATAAC 1055 0.0 21.861229 1 GTCTCGC 1360 0.0 21.803822 1 >>END_MODULE