FastQCFastQC Report
Wed 25 May 2016
SRR2049498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6033543
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.041.113521
GTATCAA84800.034.025951
ATCAACG88050.031.5411783
TCAACGC88050.031.1920154
CAACGCA95200.028.7949735
TATCAAC105150.026.8991012
AACGCAG103100.026.8862536
GTACATG147050.023.6996331
ACGCAGA122400.022.1861537
TACATGG151050.022.1852842
CGCAGAG124450.021.7265688
ACATGGG155350.020.812593
CATGGGG101550.017.3070164
GTGGTAT35000.017.1322941
GCAGAGT145200.017.1269869
GAGTACA113400.017.038291
AGAGTAC118750.016.12518310-11
GTACTAG14400.015.6598611
CAGAGTA135900.015.08838310-11
TGGTATC37300.014.6988362
Produced by FastQC (version 0.11.5)