##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049498_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6033543
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.73040649581846	31.0	31.0	34.0	27.0	34.0
2	30.861990044655354	31.0	31.0	34.0	27.0	34.0
3	31.072108875332454	31.0	31.0	34.0	28.0	34.0
4	34.67785611207213	37.0	35.0	37.0	32.0	37.0
5	34.676951005404284	37.0	35.0	37.0	32.0	37.0
6	34.61978674884724	37.0	35.0	37.0	32.0	37.0
7	30.73172064904485	37.0	33.0	37.0	2.0	37.0
8	32.508002677697	37.0	33.0	37.0	17.0	37.0
9	35.29204548637509	39.0	34.0	39.0	28.0	39.0
10-11	35.850661046088504	39.0	36.0	39.0	30.5	39.0
12-13	35.9993009082723	39.0	37.0	39.0	31.0	39.0
14-15	37.19233798449767	40.0	37.0	41.0	31.0	41.0
16-17	37.20492279577688	40.0	37.0	41.0	31.0	41.0
18-19	37.202162808817306	40.0	37.0	41.0	31.0	41.0
20-21	37.27318509207608	40.0	37.5	41.0	31.0	41.0
22-23	37.232438800883656	40.0	37.0	41.0	31.0	41.0
24-25	37.217429046250274	40.0	37.0	41.0	31.0	41.0
26-27	37.247195387519405	40.0	37.0	41.0	31.0	41.0
28-29	37.235198622103134	40.0	37.0	41.0	31.0	41.0
30-31	37.15875324001171	40.0	37.0	41.0	31.0	41.0
32-33	37.12701392863198	40.0	37.0	41.0	31.0	41.0
34-35	37.07101780827617	40.0	37.0	41.0	30.5	41.0
36-37	36.91606855209286	40.0	37.0	41.0	30.0	41.0
38-39	36.799652045241075	40.0	37.0	41.0	30.0	41.0
40-41	36.727593306287865	40.0	37.0	41.0	30.0	41.0
42-43	36.6080062742571	40.0	36.5	41.0	30.0	41.0
44-45	36.479575184928656	40.0	36.0	41.0	30.0	41.0
46-47	36.336071442600144	40.0	36.0	41.0	30.0	41.0
48-49	36.26034487530792	40.0	36.0	41.0	29.5	41.0
50-51	36.143179223219256	39.5	35.5	41.0	29.0	41.0
52-53	36.026917103267515	39.0	35.0	41.0	29.0	41.0
54-55	35.90839379117709	39.0	35.0	41.0	29.0	41.0
56-57	35.714674611583945	39.0	35.0	41.0	28.0	41.0
58-59	35.5413397236085	39.0	35.0	41.0	28.0	41.0
60-61	35.329495704265305	39.0	35.0	40.0	28.0	41.0
62-63	35.090822755386014	38.0	34.0	40.0	28.0	41.0
64-65	34.74620840524382	38.0	34.0	40.0	26.5	41.0
66-67	31.246493726820212	36.5	30.5	40.0	6.5	41.0
68-69	31.5980875581727	36.0	30.5	39.5	11.0	41.0
70-71	31.75986804104984	36.0	30.0	39.0	15.5	41.0
72-73	32.998814543958666	35.0	32.0	39.0	24.0	41.0
74-75	33.08058648127643	35.0	33.0	39.0	25.0	40.0
76-77	32.77383802518686	35.0	33.0	37.5	24.5	40.0
78-79	29.377520554672437	35.0	29.0	37.0	6.5	39.0
80-81	31.149041864788234	35.0	30.0	36.0	19.5	39.0
82-83	31.55386354253214	35.0	32.0	36.0	22.0	38.5
84-85	29.71297478115263	35.0	30.5	36.0	11.0	37.0
86-87	29.333495344277814	34.0	28.5	35.0	12.0	37.0
88-89	30.55028488236514	34.0	30.5	35.0	19.0	36.5
90-91	30.702754086612128	35.0	31.5	35.0	18.5	36.0
92-93	30.54665036778556	35.0	32.0	35.0	15.5	36.0
94-95	30.33196838076732	35.0	32.0	35.0	8.0	36.0
96-97	30.141211639661805	35.0	32.0	35.0	2.0	35.5
98-99	29.944147327697838	35.0	31.5	35.0	2.0	35.0
100	29.736480373140623	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.18652963102312725
1101	2	0.16625887021082875
1101	3	0.18096694742018116
1101	4	0.1365722208128588
1101	5	0.1439565923927688
1101	6	0.11423367397655682
1101	7	-12.143841133462107
1101	8	-6.650849896894947
1101	9	-2.4288686421992765
1101	10-11	-0.35031913090318767
1101	12-13	0.12712302965733357
1101	14-15	0.3204375598029898
1101	16-17	0.33270927965145347
1101	18-19	0.3132831665025435
1101	20-21	0.2733382566679836
1101	22-23	0.2260001177932338
1101	24-25	0.3120376116169794
1101	26-27	0.2322560304134882
1101	28-29	0.19528416765285073
1101	30-31	0.21297023383840497
1101	32-33	0.3079045742457964
1101	34-35	0.2471083513037513
1101	36-37	0.3305145178020794
1101	38-39	0.32444466191526544
1101	40-41	0.3050748286479177
1101	42-43	0.3234526768264203
1101	44-45	0.2332139395455357
1101	46-47	0.25331350245139816
1101	48-49	0.1597701871830992
1101	50-51	0.23410194803889084
1101	52-53	0.16004632802194152
1101	54-55	0.10856138784105696
1101	56-57	0.052030721163696114
1101	58-59	0.07765934252878282
1101	60-61	0.07443208412362878
1101	62-63	0.10317739017671812
1101	64-65	-0.019142362371198374
1101	66-67	-10.47582615343524
1101	68-69	-8.419494714567556
1101	70-71	-6.942767646795598
1101	72-73	-1.8798331550098055
1101	74-75	-0.6553206566917353
1101	76-77	-0.3675402727793724
1101	78-79	2.749481037996251
1101	80-81	0.6719677497222065
1101	82-83	0.02493439944557707
1101	84-85	-5.626651224274472
1101	86-87	-6.148113346292774
1101	88-89	-1.652093409880159
1101	90-91	-0.36262933649835105
1101	92-93	-0.018003898018445597
1101	94-95	0.02483938185871537
1101	96-97	0.12773866070400786
1101	98-99	0.08244544572838919
1101	100	0.14559168747344842
1102	1	0.1508419586915437
1102	2	0.12899465286513134
1102	3	0.16284132716947752
1102	4	0.1314321835763721
1102	5	0.15152964890879161
1102	6	0.15462945231551828
1102	7	-12.21367830404801
1102	8	-6.650941256268403
1102	9	-2.40655232776669
1102	10-11	-0.37676151269131
1102	12-13	0.2363700520567491
1102	14-15	0.35542200328776374
1102	16-17	0.3463669498825652
1102	18-19	0.33547797296844095
1102	20-21	0.269822787698871
1102	22-23	0.2840275402227732
1102	24-25	0.2857615436100147
1102	26-27	0.26304977971637555
1102	28-29	0.2631348676880876
1102	30-31	0.2548123899450445
1102	32-33	0.29924851193965907
1102	34-35	0.3120770603211227
1102	36-37	0.3867398170079994
1102	38-39	0.3516764681329789
1102	40-41	0.29188293233556806
1102	42-43	0.23200627363700477
1102	44-45	0.19027995948317056
1102	46-47	0.23852309515383752
1102	48-49	0.11314520899018277
1102	50-51	0.17850718739567384
1102	52-53	0.21671150275031437
1102	54-55	0.16267997961654856
1102	56-57	0.1951618229768073
1102	58-59	0.12741556394323084
1102	60-61	0.11496815868304111
1102	62-63	0.08323586335345112
1102	64-65	-0.049473992130273814
1102	66-67	-10.497144148244754
1102	68-69	-7.016161312913301
1102	70-71	-6.52392654759775
1102	72-73	-1.4103990619795894
1102	74-75	-0.5368670721789144
1102	76-77	-0.3733007196045577
1102	78-79	2.7818655128768732
1102	80-81	0.6644865689587078
1102	82-83	-0.027919381386620756
1102	84-85	-5.7096933595352795
1102	86-87	-6.196420886094479
1102	88-89	-1.7668094824249003
1102	90-91	-0.46085369160444856
1102	92-93	-0.05374066608289496
1102	94-95	-0.035540464994376464
1102	96-97	0.09076881914817037
1102	98-99	0.1264008633074596
1102	100	0.18402848251671955
1103	1	0.0156294218593942
1103	2	0.05174600990297051
1103	3	0.10498907806617908
1103	4	0.06637907466166126
1103	5	0.12172757833594261
1103	6	0.13301521265533012
1103	7	-12.2248013502393
1103	8	-6.711739571489687
1103	9	-2.5126660498241336
1103	10-11	-0.5280231997282172
1103	12-13	0.01909327481572376
1103	14-15	0.19198644594211345
1103	16-17	0.1477787452558701
1103	18-19	0.1701273436501083
1103	20-21	0.19880556172581265
1103	22-23	0.22912144576412175
1103	24-25	0.28658250192781765
1103	26-27	0.2622850484752277
1103	28-29	0.21782846527806043
1103	30-31	0.19144424691671702
1103	32-33	0.2840636348834593
1103	34-35	0.2894776580329079
1103	36-37	0.3231560862658398
1103	38-39	0.32705120907866814
1103	40-41	0.2889444795104623
1103	42-43	0.31972005793628
1103	44-45	0.30393760745826626
1103	46-47	0.33935745387884
1103	48-49	0.2114696218712453
1103	50-51	0.224670123194727
1103	52-53	0.16091298366477247
1103	54-55	0.11642311702976116
1103	56-57	0.12840634636341974
1103	58-59	0.13533290796532782
1103	60-61	0.03214372718608871
1103	62-63	-0.04845147858851817
1103	64-65	-0.0801426881315308
1103	66-67	-10.541396387817528
1103	68-69	-8.56598705197434
1103	70-71	-7.104516452341038
1103	72-73	-1.997580497523881
1103	74-75	-0.7715478875307085
1103	76-77	-0.46698785302830004
1103	78-79	2.672917837394774
1103	80-81	0.6047034016103368
1103	82-83	-0.04548895170754719
1103	84-85	-5.7056782454542585
1103	86-87	-6.227920189819638
1103	88-89	-1.766451518546205
1103	90-91	-0.5194326181311659
1103	92-93	-0.11287150575675753
1103	94-95	0.010575703434124506
1103	96-97	0.1093248926437127
1103	98-99	0.07171336646912607
1103	100	0.1993579868443085
1104	1	-0.0475426727278041
1104	2	-0.037149318877943927
1104	3	0.008113039406477895
1104	4	0.025777218906846144
1104	5	0.009233162267399564
1104	6	0.03340726278425876
1104	7	-12.31621869012703
1104	8	-6.774882414028152
1104	9	-2.6377274609574997
1104	10-11	-0.6525404743045371
1104	12-13	-0.010996206171775214
1104	14-15	0.12701049318533109
1104	16-17	0.09159404454975117
1104	18-19	0.15725378953708713
1104	20-21	0.16354162951401463
1104	22-23	0.18180029751400895
1104	24-25	0.23751535157882842
1104	26-27	0.22427873366650175
1104	28-29	0.24803822487587723
1104	30-31	0.16531670459269776
1104	32-33	0.18107780619896374
1104	34-35	0.13940518199414953
1104	36-37	0.2465557569471173
1104	38-39	0.2631651101367325
1104	40-41	0.2513287310310872
1104	42-43	0.13579913997186566
1104	44-45	0.13169198157827822
1104	46-47	0.027765133444027867
1104	48-49	0.004630226399051196
1104	50-51	0.08497291278637675
1104	52-53	0.0246673393735648
1104	54-55	0.03525102559185456
1104	56-57	-0.003938013357078773
1104	58-59	-0.0817615411465269
1104	60-61	-0.10884688405697318
1104	62-63	-0.17385430291924564
1104	64-65	-0.24648578958999678
1104	66-67	-10.652663992342426
1104	68-69	-8.678840365849911
1104	70-71	-7.131842838100827
1104	72-73	-2.1510975385683224
1104	74-75	-0.8735846658953861
1104	76-77	-0.58394487201803
1104	78-79	2.6244592386732855
1104	80-81	0.5471342311136524
1104	82-83	-0.0957218476773285
1104	84-85	-5.8089705847363575
1104	86-87	-6.299102209996406
1104	88-89	-1.8397344111728984
1104	90-91	-0.5571096566174134
1104	92-93	-0.19741000532894049
1104	94-95	-0.15256843515784269
1104	96-97	-0.043242540899022686
1104	98-99	-5.959174407195178E-4
1104	100	0.11879834125074495
1105	1	-0.11263265226822128
1105	2	-0.11834412891003154
1105	3	-0.08987756570573424
1105	4	-0.07318030172940837
1105	5	-0.025583196909330752
1105	6	-0.08171474677547508
1105	7	-12.180755855909606
1105	8	-6.74111335669275
1105	9	-2.6417785174921917
1105	10-11	-0.6120748369124129
1105	12-13	-0.11932302916036264
1105	14-15	0.030864792634531568
1105	16-17	0.09123214829544679
1105	18-19	0.0917993709814624
1105	20-21	0.0884775563231841
1105	22-23	0.1406118321087959
1105	24-25	0.13240754114674047
1105	26-27	0.10020278613294664
1105	28-29	0.07990512168871788
1105	30-31	0.08887235063546939
1105	32-33	0.09728126074131893
1105	34-35	0.12004488101543132
1105	36-37	0.18842619082805356
1105	38-39	0.18655410889043367
1105	40-41	0.09756398060658
1105	42-43	0.042201654113561915
1105	44-45	0.007191708626045568
1105	46-47	0.03024290489120318
1105	48-49	0.022880884053478212
1105	50-51	0.11907051236746469
1105	52-53	0.10703343276163224
1105	54-55	0.09224808107472171
1105	56-57	0.06543397555368102
1105	58-59	0.07438251450100353
1105	60-61	0.005375373939273231
1105	62-63	-0.08184711234212472
1105	64-65	-0.2676764926248083
1105	66-67	-10.435529641799512
1105	68-69	-8.597248429172723
1105	70-71	-7.030520444601738
1105	72-73	-2.111623262277533
1105	74-75	-0.9575308484484353
1105	76-77	-0.6520606142858867
1105	78-79	2.5229199915093865
1105	80-81	0.48019317424050456
1105	82-83	-0.22072425453205113
1105	84-85	-5.752968590510356
1105	86-87	-6.274573387976869
1105	88-89	-1.9366511294400723
1105	90-91	-0.6796853401422354
1105	92-93	-0.3111867014688343
1105	94-95	-0.28908095615096485
1105	96-97	-0.19970468716810075
1105	98-99	-0.18313279668044657
1105	100	-0.1029853687005513
1106	1	0.0026173442334211927
1106	2	0.06767313692517973
1106	3	0.03795578228493923
1106	4	0.08324599171951519
1106	5	0.03651050240602416
1106	6	-0.001311936529390323
1106	7	-12.44293561985392
1106	8	-6.873685264221383
1106	9	-2.7655785946500586
1106	10-11	-0.5789003237684582
1106	12-13	-0.00836957382281156
1106	14-15	0.13273748991287704
1106	16-17	0.109712704134445
1106	18-19	0.14503217232997656
1106	20-21	0.09407502574118354
1106	22-23	0.12465611790669584
1106	24-25	0.19982762570580093
1106	26-27	0.16025313996725288
1106	28-29	0.12746017021267875
1106	30-31	0.1350991990613153
1106	32-33	0.17925307023431714
1106	34-35	0.25246173738484146
1106	36-37	0.25960269103740075
1106	38-39	0.2521853341102087
1106	40-41	0.2074170119231269
1106	42-43	0.19449039001455048
1106	44-45	0.06623959236669208
1106	46-47	0.19367428305802292
1106	48-49	0.13547417393367311
1106	50-51	0.12060667304541539
1106	52-53	0.13694829660107644
1106	54-55	0.08046363119429856
1106	56-57	0.10542027017519473
1106	58-59	0.062345672384452655
1106	60-61	0.05265867436764182
1106	62-63	-0.05491887293986508
1106	64-65	-0.1568068445837838
1106	66-67	-10.659254981847234
1106	68-69	-8.620255140804229
1106	70-71	-7.133766335414055
1106	72-73	-2.1093608227671474
1106	74-75	-0.7945434293485292
1106	76-77	-0.5347533299558762
1106	78-79	2.6169137814959633
1106	80-81	0.48361946779156995
1106	82-83	-0.13985981469828346
1106	84-85	-5.900994492020999
1106	86-87	-6.375487638250888
1106	88-89	-1.922829769008132
1106	90-91	-0.6139625448537913
1106	92-93	-0.2315936709265145
1106	94-95	-0.1611377547204107
1106	96-97	-0.11007117479051587
1106	98-99	-0.16263371580287966
1106	100	-0.07847850135397039
1107	1	0.018121805413979786
1107	2	0.07587348711008346
1107	3	0.0649189800245118
1107	4	-0.009803389940671536
1107	5	0.0947667450308316
1107	6	0.010650048863581674
1107	7	-12.373261168110893
1107	8	-6.84238864338549
1107	9	-2.7093056802148467
1107	10-11	-0.6403717268082616
1107	12-13	0.03091783986723584
1107	14-15	0.07387780561435164
1107	16-17	0.1200657931714133
1107	18-19	0.13787348332778748
1107	20-21	0.11972118098664453
1107	22-23	0.18509872325295618
1107	24-25	0.22481820655243467
1107	26-27	0.15427151816833629
1107	28-29	0.11401044034760588
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2216	80-81	0.8573196350732672
2216	82-83	-2.228613626478193E-4
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2216	88-89	0.5044279479223732
2216	90-91	0.1500604370849885
2216	92-93	0.023492023460583766
2216	94-95	0.06760613381254998
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2216	98-99	0.021176180107495668
2216	100	-1.0334175672442854
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89307.0
3	16419.0
4	9806.0
5	8523.0
6	7988.0
7	8874.0
8	9739.0
9	11348.0
10	12794.0
11	14423.0
12	16292.0
13	18083.0
14	20801.0
15	22859.0
16	24287.0
17	24928.0
18	23861.0
19	22626.0
20	22507.0
21	22154.0
22	23672.0
23	27400.0
24	32584.0
25	41147.0
26	51074.0
27	62665.0
28	77088.0
29	98372.0
30	121065.0
31	149863.0
32	200013.0
33	282443.0
34	431230.0
35	583219.0
36	647300.0
37	1098699.0
38	1267427.0
39	429666.0
40	997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66485840255987	20.999043678322497	14.434224413836477	22.901873505281152
2	15.295139999608843	20.681628554768857	40.73435527119651	23.288876174425784
3	18.066824232941737	25.543885411970617	30.761605319684953	25.627685035402685
4	12.607911612172884	17.350122070272036	39.26275522465736	30.77921109289771
5	12.716301988678037	37.903687884370434	35.723907146959306	13.656102979992227
6	30.07659631456583	38.37832874409591	18.10670047448179	13.438374466856468
7	26.335540216931964	32.816453021552334	23.01845330328215	17.829553458233555
8	23.98478373068423	36.97120199047259	21.126744456241457	17.917269822601718
9	24.10886936581044	17.651950106264263	22.368581776909522	35.870598751015784
10-11	23.081869143884447	27.626827222413098	29.43597982147471	19.85532381222774
12-13	23.57524426361095	25.167666825279937	29.404737813255	21.852351097854115
14-15	21.190293994755653	27.612871906274638	26.500440951527153	24.696393147442556
16-17	20.603714975289737	30.84362278375862	27.21416133783131	21.33850090312033
18-19	20.520836596341486	29.818217919388328	29.13250141749218	20.52844406677801
20-21	21.735297154590594	29.18683102117611	28.50477737541607	20.57309444881722
22-23	21.370445192816227	29.108419381448016	28.54088385547265	20.98025157026311
24-25	21.221826048144514	29.13818630280749	28.560656649003747	21.079331000044252
26-27	20.96638420082963	29.03574556329526	28.618983043431278	21.37888719244383
28-29	20.988679454178083	29.104913978403737	28.775406092241322	21.131000475176855
30-31	21.37177940059431	29.540570109469677	28.24749073637165	20.840159753564365
32-33	20.87117318483902	29.31632033057862	28.73724919790067	21.075257286681687
34-35	21.002333372530877	28.97500580712008	28.78805921861978	21.234601601729267
36-37	21.43522826227115	29.262769696473477	28.26880959807688	21.033192443178482
38-39	21.239391491713448	29.374845602702827	28.437999473443583	20.947763432140146
40-41	21.031042622883437	29.14361429097298	28.67163621772481	21.153706868418773
42-43	20.969406020346266	29.100387169058294	28.61598663471196	21.314220175883477
44-45	21.193131884182275	29.211378243176778	28.50395684451853	21.091533028122413
46-47	21.095949056291367	29.422118202703146	28.358379397107775	21.12355334389771
48-49	21.01265392696862	29.13609466474904	28.5781268840114	21.273124524270937
50-51	21.01352028872011	29.037246054338052	28.551021169217	21.398212487724837
52-53	21.02508813248629	29.105449048898407	28.52195978142149	21.347503037193817
54-55	21.1452819776719	29.43580101726712	28.34243402087853	21.076482984182444
56-57	21.021218642412624	29.1873678620654	28.32443132208541	21.466982173436573
58-59	20.853565772377323	28.845254544006576	28.726750011539693	21.574429672076413
60-61	21.14929078837019	29.201933882051094	28.33557361779145	21.313201711787265
62-63	20.985506093374422	29.26745668588884	28.36181438401829	21.385222836718448
64-65	20.973276235344816	28.96910783357055	28.55000528301418	21.507610648070457
66-67	20.90887599805067	28.99090622142649	28.380469220837025	21.71974855968582
68-69	21.470109750081924	29.122320364144894	28.174163788016255	21.233406097756934
70-71	21.20090181585537	28.897736883083414	28.355462296647033	21.54589900441419
72-73	20.958189976762124	29.02954925022825	28.60452016870227	21.407740604307357
74-75	20.993810006403557	29.014216192568444	28.484296453729442	21.50767734729856
76-77	21.072380273278903	29.044092357973256	28.246618655477867	21.63690871326997
78-79	21.24188820642171	29.068010389198612	28.34263241024164	21.347468994138037
80-81	21.01376964545303	28.878721687346975	28.48766279513916	21.619845872060832
82-83	20.930653673788637	28.90213970769339	28.602052888872244	21.56515372964573
84-85	21.034317172215562	28.820108271406475	28.426544543930078	21.719030012447885
86-87	21.142704987938245	28.89544562040381	28.39219902725272	21.56965036440523
88-89	20.961948420155526	28.7151844668433	28.485721748879	21.837145364122172
90-91	20.900454939628677	28.63210818307906	28.480384292052413	21.987052585239855
92-93	21.088125811071404	28.835353054269387	28.282065512897958	21.79445562176126
94-95	20.976803500450252	28.579855637421826	28.497553818560746	21.945787043567176
96-97	20.925613848696763	28.568568278223655	28.543441265400343	21.962376607679236
98-99	21.017547171734645	28.546178976991378	28.42733646329486	22.00893738797912
100	21.100545784359486	28.529879572071117	28.30371255357067	22.065862089998728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3603.0
1	2678.5
2	2017.0
3	2222.5
4	1970.5
5	1677.0
6	1500.5
7	1405.0
8	1422.5
9	1401.5
10	1270.0
11	1149.5
12	1183.0
13	1285.0
14	1340.0
15	1533.0
16	1900.5
17	2435.5
18	3269.0
19	4546.5
20	6511.0
21	9231.5
22	13395.0
23	19880.5
24	28214.0
25	38894.0
26	53058.5
27	70108.5
28	90411.0
29	111071.0
30	128974.5
31	145711.5
32	160943.5
33	174715.5
34	187962.0
35	199809.5
36	208701.0
37	215719.0
38	220993.0
39	222787.0
40	223015.0
41	220824.0
42	215937.0
43	211061.5
44	205801.5
45	200762.5
46	196065.5
47	190431.5
48	184433.0
49	179206.5
50	173283.5
51	165020.0
52	155026.0
53	146027.5
54	138994.0
55	131078.5
56	120439.0
57	108417.5
58	96756.5
59	85583.5
60	73060.0
61	59635.0
62	48397.0
63	38920.5
64	30506.0
65	23543.5
66	17978.5
67	13443.0
68	9781.0
69	7124.5
70	5223.0
71	3763.0
72	2725.0
73	1919.5
74	1279.5
75	863.5
76	569.5
77	369.5
78	247.0
79	170.5
80	132.5
81	97.0
82	70.0
83	61.0
84	47.0
85	35.5
86	33.5
87	27.5
88	23.0
89	24.0
90	15.0
91	11.5
92	14.0
93	13.5
94	12.5
95	12.0
96	13.0
97	16.5
98	17.5
99	25.0
100	38.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.017469006187575027
2	0.002801007633491632
3	0.0017071229955599888
4	0.002204343285528917
5	0.0022872133338570724
6	0.0037125781651013347
7	11.755829037764379
8	1.6574009665630957E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	2.8175816431572624E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.8231410632194053E-4
28-29	0.0
30-31	0.0
32-33	1.6574009665630957E-5
34-35	2.4861014498446434E-5
36-37	1.6574009665630957E-5
38-39	2.4861014498446434E-5
40-41	0.0
42-43	4.972202899689287E-5
44-45	1.7402710148912504E-4
46-47	9.944405799378574E-4
48-49	6.712473914580537E-4
50-51	2.983321739813572E-4
52-53	2.1546212565320245E-4
54-55	4.060632368079584E-4
56-57	0.0012430507249223217
58-59	3.066191788141727E-4
60-61	0.0013756428022473695
62-63	0.002411518406349304
64-65	9.198575364425181E-4
66-67	5.878146555017508
68-69	5.518399388220155
70-71	0.0026601285513337685
72-73	0.0012181897104238755
74-75	0.002925312705983864
76-77	0.00606608753762093
78-79	5.490546764977062
80-81	0.005113081981847151
82-83	0.004789888793367347
84-85	5.8782377120706695
86-87	0.0036214211119403638
88-89	0.0016739749762287267
90-91	0.0035634120781106557
92-93	0.004019197343915507
94-95	0.004342390532395311
96-97	0.003240218889630852
98-99	0.005776042368472388
100	0.006480437779261704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6033543.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.9508419514625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.6108346031744	85.1398422293781
2	4.801936048732214	8.734802532585476
3	0.8966900086504423	2.4466413376866574
4	0.30724533439892915	1.11776887396965
5	0.1540043291652395	0.7003411700874352
6	0.07663111901155545	0.4181798876270216
7	0.046304694560942064	0.29480156696360904
8	0.03344491897242227	0.24334748316321977
9	0.017597391319151563	0.14404478009635727
>10	0.05477236435255758	0.7175757320950222
>50	5.203917818518166E-4	0.032380304500995306
>100	0.0	0.0
>500	1.8795880307363773E-5	0.01027410184643363
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6574009665630957E-5	0.0
5	1.6574009665630957E-5	0.0	0.0	1.6574009665630957E-5	0.0
6	1.6574009665630957E-5	0.0	0.0	1.6574009665630957E-5	0.0
7	1.6574009665630957E-5	0.0	0.0	3.3148019331261914E-5	0.0
8	1.6574009665630957E-5	0.0	0.0	3.3148019331261914E-5	0.0
9	1.6574009665630957E-5	0.0	0.0	3.3148019331261914E-5	0.0
10-11	3.3148019331261914E-5	1.6574009665630957E-5	0.0	4.1435024164077395E-5	0.0
12-13	3.3148019331261914E-5	2.4861014498446434E-5	0.0	4.972202899689287E-5	0.0
14-15	3.3148019331261914E-5	4.1435024164077395E-5	0.0	4.972202899689287E-5	0.0
16-17	3.3148019331261914E-5	7.45830434953393E-5	0.0	4.972202899689287E-5	0.0
18-19	3.3148019331261914E-5	1.0773106282660122E-4	0.0	6.629603866252383E-5	0.0
20-21	3.3148019331261914E-5	1.160180676594167E-4	0.0	9.115705316097026E-5	0.0
22-23	3.3148019331261914E-5	1.2430507249223217E-4	0.0	2.4861014498446434E-4	0.0
24-25	3.3148019331261914E-5	1.4087908215786314E-4	0.0	5.71803333464268E-4	0.0
26-27	3.3148019331261914E-5	1.491660869906786E-4	0.0	0.0010110145896034884	0.0
28-29	3.3148019331261914E-5	1.6574009665630958E-4	0.0	0.002701563575497846	0.0
30-31	4.972202899689287E-5	1.7402710148912504E-4	0.0	0.007847793576676258	0.0
32-33	6.629603866252383E-5	1.8231410632194053E-4	0.0	0.0196484884586055	0.0
34-35	6.629603866252383E-5	1.8231410632194053E-4	0.0	0.03902350575772809	0.0
36-37	6.629603866252383E-5	1.9888811598757147E-4	0.0	0.06974343267297506	0.0
38-39	6.629603866252383E-5	2.320361353188334E-4	0.0	0.11144364099170255	1.6574009665630957E-5
40-41	6.629603866252383E-5	2.817581643157263E-4	0.0	0.15994748027817154	1.6574009665630957E-5
42-43	6.629603866252383E-5	2.983321739813572E-4	0.0	0.21118603115946966	1.6574009665630957E-5
44-45	6.629603866252383E-5	2.983321739813572E-4	0.0	0.2728579211252824	1.6574009665630957E-5
46-47	6.629603866252383E-5	3.149061836469882E-4	0.0	0.3515264580031998	1.6574009665630957E-5
48-49	6.629603866252383E-5	3.149061836469882E-4	0.0	0.43481085657299534	1.6574009665630957E-5
50-51	6.629603866252383E-5	3.81202222309512E-4	0.0	0.5199101092011775	1.6574009665630957E-5
52-53	6.629603866252383E-5	3.9777623197514295E-4	0.0	0.6068242158877462	1.6574009665630957E-5
54-55	6.629603866252383E-5	3.9777623197514295E-4	0.0	0.7067157721425039	1.6574009665630957E-5
56-57	6.629603866252383E-5	3.9777623197514295E-4	0.0	0.8172064075784328	1.6574009665630957E-5
58-59	6.629603866252383E-5	3.9777623197514295E-4	0.0	0.939398293838297	1.6574009665630957E-5
60-61	6.629603866252383E-5	4.3921125613922035E-4	0.0	1.066131458746544	1.6574009665630957E-5
62-63	6.629603866252383E-5	4.474982609720358E-4	0.0	1.1969252560228707	1.6574009665630957E-5
64-65	6.629603866252383E-5	4.474982609720358E-4	0.0	1.3439284347521845	1.6574009665630957E-5
66-67	6.629603866252383E-5	4.474982609720358E-4	0.0	1.5101326036791318	1.6574009665630957E-5
68-69	6.629603866252383E-5	4.474982609720358E-4	0.0	1.6783753757949516	1.6574009665630957E-5
70-71	6.629603866252383E-5	4.474982609720358E-4	0.0	1.8850284153108712	1.6574009665630957E-5
72-73	6.629603866252383E-5	5.055072948017442E-4	0.0	2.111860311594697	1.6574009665630957E-5
74-75	6.629603866252383E-5	5.220813044673751E-4	0.0	2.320137604057848	1.6574009665630957E-5
76-77	7.45830434953393E-5	5.303683093001906E-4	0.0	2.5422459075869686	1.6574009665630957E-5
78-79	8.287004832815479E-5	5.303683093001906E-4	0.0	2.8040323902556095	1.6574009665630957E-5
80-81	8.287004832815479E-5	5.303683093001906E-4	0.0	3.123181520376999	1.6574009665630957E-5
82-83	9.115705316097026E-5	5.303683093001906E-4	0.0	3.4563688366851784	1.6574009665630957E-5
84-85	9.944405799378574E-5	5.552293237986371E-4	0.0	3.7917770702885516	1.6574009665630957E-5
86-87	9.944405799378574E-5	5.966643479627144E-4	0.0	4.194409487095725	1.6574009665630957E-5
88	9.944405799378574E-5	6.298123672939764E-4	0.0	4.528649253017671	1.6574009665630957E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5435	0.0	41.11352	1
GTATCAA	8480	0.0	34.02595	1
ATCAACG	8805	0.0	31.541178	3
TCAACGC	8805	0.0	31.192015	4
CAACGCA	9520	0.0	28.794973	5
TATCAAC	10515	0.0	26.899101	2
AACGCAG	10310	0.0	26.886253	6
GTACATG	14705	0.0	23.699633	1
ACGCAGA	12240	0.0	22.186153	7
TACATGG	15105	0.0	22.185284	2
CGCAGAG	12445	0.0	21.726568	8
ACATGGG	15535	0.0	20.81259	3
CATGGGG	10155	0.0	17.307016	4
GTGGTAT	3500	0.0	17.132294	1
GCAGAGT	14520	0.0	17.126986	9
GAGTACA	11340	0.0	17.03829	1
AGAGTAC	11875	0.0	16.125183	10-11
GTACTAG	1440	0.0	15.659861	1
CAGAGTA	13590	0.0	15.088383	10-11
TGGTATC	3730	0.0	14.698836	2
>>END_MODULE